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host switch during evolution of a genetically distinct hantavirus in the american shrew mole (neurotrichus gibbsii).a genetically distinct hantavirus, designated oxbow virus (oxbv), was detected in tissues of an american shrew mole (neurotrichus gibbsii), captured in gresham, oregon, in september 2003. pairwise analysis of full-length s- and m- and partial l-segment nucleotide and amino acid sequences of oxbv indicated low sequence similarity with rodent-borne hantaviruses. phylogenetic analyses using maximum-likelihood and bayesian methods, and host-parasite evolutionary comparisons, showed that oxbv and asa ...200919394994
novel serological tools for detection of thottapalayam virus, a soricomorpha-borne hantavirus.we developed serological tools for the detection of hantavirus-specific antibodies and hantavirus antigens in shrews. the work was focussed to generate thottapalayam virus (tpmv)-specific monoclonal antibodies (mabs) and anti-shrew immunoglobulin g (igg) antibodies. the mabs against tpmv nucleocapsid (n) protein were produced after immunization of balb/c mice with recombinant tpmv n proteins expressed in escherichia coli, baculovirus and saccharomyces cerevisiae-mediated expression systems. in t ...201222828778
molecular phylogeny of a newfound hantavirus in the japanese shrew mole (urotrichus talpoides).recent molecular evidence of genetically distinct hantaviruses in shrews, captured in widely separated geographical regions, corroborates decades-old reports of hantavirus antigens in shrew tissues. apart from challenging the conventional view that rodents are the principal reservoir hosts, the recently identified soricid-borne hantaviruses raise the possibility that other soricomorphs, notably talpids, similarly harbor hantaviruses. in analyzing rna extracts from lung tissues of the japanese sh ...200818854415
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