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spontaneous cryptosporidiosis in an adult female rabbit.an asymptomatic adult female rabbit had intestinal cryptosporidiosis. the ileum had blunted villi, a decrease in villus-crypt ratio and a mild edema in the lamina propria. transmission electron microscopy showed the parasite to be a cryptosporidium similar to those reported in mouse, guinea pig, lamb, calf, horse, monkey and man. this organism is referred to as cryptosporidium cuniculus. scanning electron microscopy on ileal mucosa showed altered intestinal microvilli in the attachment of the cr ...1979462721
cryptosporidium cuniculus in the rabbit (oryctolagus cuniculus).cryptosporidium cuniculus was identified by light and electron microscopy in two apparently healthy rabbits. organisms were firmly attached at the brush borders of intestinal epithelial cells. cellular alterations were minimal, consisting of elongation and shortening of microvilli adjacent to the attachment sites of organisms. various developmental stages of cryptosporidium were identified by electron microscopy.1979513637
re-description of cryptosporidium cuniculus inman and takeuchi, 1979 (apicomplexa: cryptosporidiidae): morphology, biology and phylogeny.to provide re-description of cryptosporidium cuniculus inman and takeuchi, 1979 (synonymous with rabbit genotype), a species closely related to cryptosporidium hominis, the morphology, natural and experimental host specificity, and genetic characterisation were investigated. the morphology and diagnostic characteristics are typical of other intestinal species of cryptosporidium, albeit with slightly larger oocysts (5.55-6.40×5.02-5.92 μm; mean 5.98×5.38 μm; length:width=1.1; n=50). natural hosts ...201020600069
molecular detection of cryptosporidium cuniculus in rabbits in australia.in the united kingdom, rabbits have been reported to harbour genotypes of cryptosporidium (now recognized as c. cuniculus) identical to those from human patients exhibiting symptoms of cryptosporidiosis. the high density of rabbits in many regions of australia, including both rural and urban as well as natural water catchments areas, and the absence of any information on cryptosporidium from lagomorphs in this country stimulated the present study. we undertook an epidemiological study that genet ...201020691809
sporadic human cryptosporidiosis caused by cryptosporidium cuniculus, united kingdom, 2007-2008.to investigate sporadic human cryptosporidiosis trends in the united kingdom, we tested 3,030 cryptosporidium spp.-positive fecal samples, submitted for routine typing in 2007-2008, for c. cuniculus. c. cuniculus prevalence was 1.2%; cases were mostly indigenous and occurred across all age groups. most occurred during august-october and may be linked to exposure opportunities.201121392453
molecular-based investigation of cryptosporidium and giardia from animals in water catchments in southeastern australia.there has been no large-scale systematic molecular epidemiological investigation of the waterborne protozoans, cryptosporidium or giardia, in southeastern australia. here, we explored, for the first time, the genetic composition of these genera in faecal samples from animals in nine melbourne water reservoir areas, collected over a period of two-years. we employed pcr-based single-strand conformation polymorphism (sscp) and phylogenetic analyses of loci (pssu and pgp60) in the small subunit (ssu ...201223357792
subtyping of cryptosporidium cuniculus and genotyping of enterocytozoon bieneusi in rabbits in two farms in heilongjiang province, china.cryptosporidium spp. and enterocytozoon bieneusi are two prevalent opportunistic pathogens in humans and animals. currently, few data are available on genetic characterization of both pathogens in rabbits in china. the aim of the present study was to understand prevalence and genetic characterization of cryptosporidium spp. and e. bieneusi in rabbits. we collected 215 fecal samples from 150 rex rabbits and 65 new zealand white rabbits on two different farms in heilongjiang province, china. crypt ...201627882867
occurrence of giardia intestinalis and cryptosporidium sp. in wastewater samples from são paulo state, brazil, and lima, peru.the objectives of the study were to detect and genotype cryptosporidium spp. and giardia intestinalis in wastewater samples obtained from five cities with high transit of people in the state of são paulo, brazil, and at the entrance of a wastewater treatment plant (wwtp) in lima, peru. samples were collected and concentrated by centrifugation. the genomic dna was extracted for molecular characterization by nested pcr for cryptosporidium and double nested pcr for giardia, followed by sequencing a ...201627638793
autochthonous cryptosporidium cuniculus infection in spain: first report in a symptomatic paediatric patient from madrid. 201626774255
cryptosporidium cuniculus--new records in human and kangaroo in australia.to date, cryptosporidium cuniculus has been found exclusively in rabbits and humans. the present study provides the first published molecular evidence for c. cuniculus in an australian human patient as well as a kangaroo.201425359081
an outbreak of massive mortality among farm rabbits associated with cryptosporidium infection.cryptosporidium in farm rabbits is not often recognised due to a low prevalence and asymptomatic course of infection. nonetheless, incidences of fatal diarrhoeic diseases are frequently noticed in the rabbitries. in this article, we report an outbreak where there was massive mortality among farm rabbits associated with cryptosporidium infection. the disease was characterised by profuse diarrhoea resulting in the death of rabbits. a pooled faecal sample was screened for a presence of parasites us ...201424856456
the first recorded outbreak of cryptosporidiosis due to cryptosporidium cuniculus (formerly rabbit genotype), following a water quality incident.we report the first identified outbreak of cryptosporidiosis with cryptosporidium cuniculus following a water quality incident in northamptonshire, uk. a standardised, enhanced cryptosporidium exposure questionnaire was administered to all cases of cryptosporidiosis after the incident. stool samples, water testing, microscopy slides and rabbit gut contents positive for cryptosporidium were typed at the cryptosporidium reference unit, singleton hospital, swansea. twenty-three people were microbio ...201424642431
new subtype of cryptosporidium cuniculus isolated from rabbits by sequencing the gp60 gene.given the paucity of literature available on rabbits infected with cryptosporidium in sichuan province (china), 290 fecal samples were collected from rabbits in the animal house of sichuan agricultural university, china and examined for cryptosporidium oocysts using the sheather's sucrose flotation technique and a modified acid-fast staining method. three samples tested positive (prevalence = 1.03%). the positive isolates were genotyped by sequence analysis of the 18s rrna, hsp70, cowp, and cp13 ...201424628387
detection and characterization of cryptosporidium cuniculus by real-time pcr.cryptosporidium cuniculus was originally detected in rabbits and has been identified as an emerging human pathogen, but the occurrence, prevalence, and epidemiology in human and rabbit populations are poorly understood. as identification of c. cuniculus can be time-consuming and costly using existing molecular assays, a real-time polymerase chain reaction (pcr)-based method targeting specific markers for this species was developed. the assay is based on amplification of the c. cuniculus-specific ...201222392139
cryptosporidium cuniculus and giardia duodenalis in rabbits: genetic diversity and possible zoonotic transmission.cryptosporidium and giardia are the two important zoonotic pathogens causing diarrhea of humans and animals worldwide. considering the human cryptosporidiosis outbreak and sporadic cases caused by c. cuniculus, the important public health significance of g. duodenalis and little obtained information regarding rabbit infected with cryptosporidium and giardia in china, the aim of this study is to determine the prevalence and molecularly characterize cryptosporidium and giardia in rabbits in heilon ...201222363600
next generation sequencing uncovers within-host differences in the genetic diversity of cryptosporidium gp60 subtypes.the extent of within-host genetic diversity of parasites has implications for our understanding of the epidemiology, disease severity and evolution of parasite virulence. as with many other species, our understanding of the within-host diversity of the enteric parasite cryptosporidium is changing. the present study compared sanger and next generation sequencing of glycoprotein 60 (gp60) amplicons from cryptosporidium hominis (n=11), cryptosporidium parvum (n=22) and cryptosporidium cuniculus (n= ...201728495122
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