| cell cycle characteristics of crenarchaeota: unity among diversity. | the hyperthermophilic archaea acidianus hospitalis, aeropyrum pernix, pyrobaculum aerophilum, pyrobaculum calidifontis, and sulfolobus tokodaii representing three different orders in the phylum crenarchaeota were analyzed by flow cytometry and combined phase-contrast and epifluorescence microscopy. the overall organization of the cell cycle was found to be similar in all species, with a short prereplicative period and a dominant postreplicative period that accounted for 64 to 77% of the generati ... | 2008 | 18502873 |
| acidianus filamentous virus 1 coat proteins display a helical fold spanning the filamentous archaeal viruses lineage. | acidianus filamentous virus 1 (afv1), a member of the lipothrixviridae family, infects the hyperthermophilic, acidophilic crenarchaeaon acidianus hospitalis. the virion, covered with a lipidic outer shell, is 9,100-a long and contains a 20.8-kb linear dsdna genome. we have identified the two major coat proteins of the virion (mcps; 132 and 140 amino acids). they bind dna and form filaments when incubated with linear dsdna. a c-terminal domain is identified in their crystal structure with a four- ... | 2009 | 19934032 |
| genomic analysis of acidianus hospitalis w1 a host for studying crenarchaeal virus and plasmid life cycles. | the acidianus hospitalis w1 genome consists of a minimally sized chromosome of about 2.13 mb and a conjugative plasmid pah1 and it is a host for the model filamentous lipothrixvirus afv1. the chromosome carries three putative replication origins in conserved genomic regions and two large regions where non-essential genes are clustered. within these variable regions, a few orphan orfb and other elements of the is200/607/605 family are concentrated with a novel class of mite-like repeat elements. ... | 2011 | 21607549 |
| unique genome replication mechanism of the archaeal virus afv1. | the exceptional genomic content and genome organization of the acidianus filamentous virus 1 (afv1) that infects the hyperthermophilic archaeon acidianus hospitalis suggest that this virus might exploit an unusual mechanism of genome replication. an analysis of replicative intermediates of the viral genome by two-dimensional (2d) agarose gel electrophoresis revealed that viral genome replication starts by the formation of a d-loop and proceeds via strand displacement replication. characterizatio ... | 2014 | 24779456 |
| acidianus tailed spindle virus: a new archaeal large tailed spindle virus discovered by culture-independent methods. | the field of viral metagenomics has expanded our understanding of viral diversity from all three domains of life (archaea, bacteria, and eukarya). traditionally, viral metagenomic studies provide information about viral gene content but rarely provide knowledge about virion morphology and/or cellular host identity. here we describe a new virus, acidianus tailed spindle virus (atsv), initially identified by bioinformatic analysis of viral metagenomic data sets from a high-temperature (80°c) acidi ... | 2016 | 26763997 |
| [adaptation of acidianus hospitalis w1 to oligotrophic and acidic hot spring environments]. | to study the adaptation of a. hospitalis w1 to oligotrophic and acidic hot spring environments at the whole genome level. | 2014 | 25803897 |
| quartet analysis of putative horizontal gene transfer in crenarchaeota. | horizontal gene transfers (hgt) between four crenarchaeota species (metallosphaera cuprina ar-4t, acidianus hospitalis w1t, vulcanisaeta moutnovskia 768-28t, and pyrobaculum islandicum dsm 4184t) were investigated with quartet analysis. strong support was found for individual genes that disagree with the phylogeny of the majority, implying genomic mosaicism. one such gene, a ferredoxin-related gene, was investigated further and incorporated into a larger phylogeny, which provided evidence for hg ... | 2014 | 24346234 |
| physiologic versatility and growth flexibility as the main characteristics of a novel thermoacidophilic acidianus strain isolated from copahue geothermal area in argentina. | a novel thermoacidophilic archaeal strain has been isolated from three geothermal acidic hot springs in copahue, argentina. one of the most striking characteristic of ale1 isolate is its metabolic versatility. it grows on sulphur, tetrathionate, iron (ii) and sucrose under aerobic conditions, but it can also develop under anaerobic conditions using iron (iii) or sulphur as electron acceptors and sulphur or hydrogen as electron donors autotrophically. a temperature of 75 °c and a ph between 2.5 a ... | 2013 | 23052926 |
| archaeal tetrathionate hydrolase goes viral: secretion of a sulfur metabolism enzyme in the form of virus-like particles. | in the course of screening for virus-host systems in extreme thermal environments, we have isolated a strain of the hyperthermophilic archaeaon acidianus hospitalis producing unusual filamentous particles with a zipper-like appearance. the particles were shown to represent a secreted form of a genuine cellular enzyme, tetrathionate hydrolase, involved in sulfur metabolism. | 2012 | 22636008 |