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bacillus safensis sp. nov., isolated from spacecraft and assembly-facility surfaces.thirteen strains of a novel spore-forming, gram-positive, mesophilic heterotrophic bacterium were isolated from spacecraft surfaces (mars odyssey orbiter) and assembly-facility surfaces at the jet propulsion laboratory in california and the kennedy space center in florida. phylogenetic analysis of 16s rrna gene sequences has placed these novel isolates within the genus bacillus, the greatest sequence similarity (99.9 %) being found with bacillus pumilus. however, these isolates share a mere 91.2 ...200616902000
carotenoids found in bacillus.to identify the diversity of pigmented aerobic spore formers found in the environment and to characterize the chemical nature of this pigmentation.201019878522
bacillus safensis lau 13: a new source of keratinase and its multi-functional biocatalytic applications.a newly isolated bacterium identified as bacillus safensis based on biochemical tests and 16s rrna analysis and its mutant variant created by exposure to ultraviolet radiation at 254 nm were investigated for keratinolytic activity. the wild-type strain produced 35.4-50.4 u/ml keratinase over a period of 120 h, while the mutant one yielded 64.4-108.5 u/ml keratinase for the same period of 120 h. the optimal conditions for the enzyme activities were ph 7.5 and 40 °c. the mutant and wild-type strai ...201426740788
effect of antibiotic supplementation on the quality of cryopreserved fish sperm of silver barb (barbodes gonionotus): sperm motility and viability, bacterial quality and fertilization.disease prevention is a key aspect in developing cryopreservation procedures for fish sperm and in improving the reproductive biotechnology for commercially important aquatic species. the purpose of this study was to evaluate the effects of an antibiotic supplementation (0.25% penicillin-streptomycin, ps and 0.25% penicillin-gentamicin, pg) on sperm motility and viability, bacterial profile and fertilization capacity of cryopreserved silver barb (barbodes gonionotus) semen. the experimental prot ...201626786020
optimization of fermentation media and growth conditions for microbial xylanase production.efficiency of cellulase-free xylanases is one of the determining factors in paper and pulp industries. use of microbes which can produce cellulase-free xylanases may help to overcome the current challenges in kraft pulp processing. isolation and screening of microorganisms from local samples offers a possibility for obtaining the potential microbes for this purpose. this research was therefore aimed to collect, screen, characterize and identify potential cellulase-free xylanase producers. a tota ...201628330199
biomedical and catalytic applications of gold and silver-gold alloy nanoparticles biosynthesized using cell-free extract of bacillus safensis lau 13: antifungal, dye degradation, anti-coagulant and thrombolytic activities.this study investigated the green biosynthesis of gold (au) and silver-gold alloy (ag-au) nanoparticles using cell-free extract of bacillus safensis lau 13 strain (genbank accession no: kj461434). the biosynthesized aunps and ag-aunps were characterized using uv-vis spectroscopy, fourier-transform infrared spectroscopy, and transmission electron microscopy. evaluation of the antifungal activities, degradation of malachite green, anti-coagulation of blood and thrombolysis of human blood clot by t ...201628113560
biomedical and catalytic applications of gold and silver-gold alloy nanoparticles biosynthesized using cell-free extract of bacillus safensis lau 13: antifungal, dye degradation, anti-coagulant and thrombolytic activities.this study investigated the green biosynthesis of gold (au) and silver-gold alloy (ag-au) nanoparticles using cell-free extract of bacillus safensis lau 13 strain (genbank accession no: kj461434). the biosynthesized aunps and ag-aunps were characterized using uv-vis spectroscopy, fourier-transform infrared spectroscopy, and transmission electron microscopy. evaluation of the antifungal activities, degradation of malachite green, anti-coagulation of blood, and thrombolysis of human blood clot by ...201627164598
dynamics of bacterial class bacilli in the deepest valley lake of kashmir-the manasbal lake.in recognition of the importance of bacteria as ecological indicators of the aquatic systems a comprehensive and systematic analysis was carried out on manasbal lake, the deepest spring fed valley lake of kashmir. the main objective envisaged was to analyze bacterial community composition (bcc) and for this purpose systematic and regular sampling of waters from ten different sampling stations, predetermined in the lake according to differences in degree of human interference and also as zones of ...201728087491
bacillus zhangzhouensis sp. nov. and bacillus australimaris sp. nov.two strains, designated dw5-4t and nh7i_1t, were isolated from aquaculture water of shrimp farm of zhangzhou city in china and surface sediment of the south china sea, respectively. cells of the strains were gram-stain-positive, oxidase- and catalase-positive, rod-shaped and motile. the phylogenetic analyses based on 16s rrna gene sequences indicated that two strains belonged to the bacillus pumilus clade. the similarities of 16s rrna gene sequences between two strains dw5-4t and nh7i_1t was 99. ...201526705002
plant growth promoting bacteria from cow dung based biodynamic preparations.indigenous formulations based on cow dung fermentation are commonly used in organic farming. three biodynamic preparations viz., panchagavya (pg), bd500 and 'cow pat pit' (cpp) showed high counts of lactobacilli (10(9) ml(-1)) and yeasts (10(4) ml(-1)). actinomycetes were present only in cpp (10(4) ml(-1)) and absent in the other two. seven bacterial isolates from these ferments were identified by a polyphasic approach: bacillus safensis (pg1), bacillus cereus (pg2, pg4 pg5), bacillus subtilis ( ...201425320439
two-stage phyto-microremediation of tannery effluent by spirodela polyrrhiza (l.) schleid. and chromium resistant bacteria.two-stage sequential treatment of tannery effluent was conducted employing a wetland plant, spirodela polyrrhiza (l.) schleid., and chromium (cr) resistant bacterial strains. the bacterial strains were isolated from cr-enriched environmental matrices and rhizosphere of spirodela polyrrhiza. the phyto-rhizoremediation of tannery effluent by spirodela and its rhizospheric bacteria (cellulomonas biazotea apbr1-6, bacillus safensis apbr2-12, staphylococcus warneri apbr3-5, microbacterium oleivorans ...201627318662
differentiation of bacillus pumilus and bacillus safensis using maldi-tof-ms.matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof-ms) despite being increasingly used as a method for microbial identification, still present limitations in which concerns the differentiation of closely related species. bacillus pumillus and bacillus safensis, are species of biotechnological and pharmaceutical significance, difficult to differentiate by conventional methodologies. in this study, using a well-characterized collection of b. pumillus and b. saf ...201425314655
survival of bacteria of laboratory animal origin on cage bedding and inactivation by hydrogen peroxide vapour.this study aims to determine the ability of laboratory animal bacteria to resist desiccation and inactivation by hydrogen peroxide vapour (hpv) on paper bedding pieces. bedding pieces were saturated with bacterial suspensions in water or 2% (w/v) bovine serum albumin (bsa) in water, and held in a mouse facility. viable counts showed variable survival rates over time for the bacterial species used ([pasteurella] pneumotropica, muribacter muris, pseudomonas aeruginosa, acinetobacter redioresistens ...201627932683
inhibition of staphylococcus aureus by antimicrobial biofilms formed by competitive exclusion microorganisms on stainless steel.the goal of this study was to develop a desiccation resistant antimicrobial surface using biofilm of competitive exclusion (ce) microorganism inhibitory to staphylococcus aureus. we isolated 161 microorganisms from soils, foods, and food-contact surfaces that are inhibitory to s. aureus. among them, three ce microorganisms (streptomyces spororaveus strain gaeunsan-18, bacillus safensis strain chamnamu-sup 5-25, and pseudomonas azotoformans strain lettuce-9) exhibiting strong antibacterial activi ...201627648758
characterization of biosurfactants from indigenous soil bacteria recovered from oil contaminated sites.three bacterial isolates (g1, g2 and g3) characterized as pseudomonas plecoglossicida, lysinibacillus fusiformis and bacillus safensis were recovered from contaminated soil of oil refinery. these bacterial isolates produced biosurfactants in msm medium in stationary phase. biosurfactants were characterized on the basis of their emulsifying properties with petrol, diesel, mobil oil and petrol engine oil. reduction in surface tension (below 40 mn m(-1)) and blood hemolysis were also included in bi ...201526521551
whole-genome sequencing and annotation of bacillus safensis rit372 and pseudomonas oryzihabitans rit370 from capsicum annuum (bird's eye chili) and capsicum chinense (yellow lantern chili), respectively.here, we report the genome sequences of bacillus safensis rit372 and pseudomonas oryzihabitans rit370 from capsicum spp. annotation revealed gene clusters for the synthesis of bacilysin, lichensin, and bacillibactin and sporulation killing factor (skfa) in bacillus safensis rit372 and turnerbactin and carotenoid in pseudomonas oryzihabitans rit370.201525883290
highly effective bacterial agents against cimbex quadrimaculatus (hymenoptera: cimbicidae): isolation of bacteria and their insecticidal activities.cimbex quadrimaculatus (hymenoptera: cimbicidae) is one of the serious pests of almonds in turkey and worldwide. since there is no effective control application against this pest, it has been a serious problem up to now. therefore, we aimed to find an effective bacterium that can be utilized as a biocontrol agent against c. quadrimaculatus in pest management. we isolated seven bacteria from dead and live c. quadrimaculatus larvae, and evaluated the larvicidal potency of all isolates on the respe ...201525358421
genome sequence of bacillus safensis cfa06, isolated from biodegraded petroleum in brazil.bacillus safensis is a microorganism recognized for its biotechnological and industrial potential due to its interesting enzymatic portfolio. here, as a means of gathering information about the importance of this species in oil biodegradation, we report a draft genome sequence of a strain isolated from petroleum.201425059859
genome sequence of salt-tolerant bacillus safensis strain vk, isolated from saline desert area of gujarat, india.bacillus safensis strain vk was isolated from the rhizosphere of a cumin plant growing in the saline desert of radhanpar, gujarat, india. here, we provide the 3.68-mb draft genome sequence of b. safensis vk, which might provide information about the salt tolerance and genes encoding enzymes for the strain's plant growth-promoting potential.201324009116
water stress amelioration and plant growth promotion in wheat plants by osmotic stress tolerant bacteria.soil microorganisms with potential for alleviation of abiotic stresses in combination with plant growth promotion would be extremely useful tools in sustainable agriculture. to this end, the present study was initiated where forty-five salt tolerant bacterial isolates with ability to grow in high salt medium were obtained from the rhizosphere of triticum aestivum and imperata cylindrica. these bacteria were tested for plant-growth-promoting rhizobacteria traits in vitro such as phosphate solubil ...201323239372
biological control of heterodera glycines by spore-forming plant growth-promoting rhizobacteria (pgpr) on soybean.heterodera glycines, the soybean cyst nematode, is the most economically important plant-parasitic nematode on soybean production in the u.s. the objectives of this study were to evaluate the potential of plant growth-promoting rhizobacteria (pgpr) strains for mortality of h. glycines j2 in vitro and for reducing nematode population density on soybean in greenhouse, microplot, and field trials. the major group causing mortality to h. glycines in vitro was the genus bacillus that consisted of 92. ...201728704498
evaluation of the biotechnological potential of a novel purified protease bs1 from bacillus safensis s406 on the chitin extraction and detergent formulation.an extracellular alkaline stable protease bs1 from a new bacteria strain, bacillus safensis s406, isolated from the sfax solar saltern, was purified and characterized. the enzyme was purified to homogeneity by ammonium sulfate precipitation, sephadex g-75 gel filtration, mono-q anion-exchange chromatography and ultrafiltration, with a 12.70-fold increase in specific activity and 20.29% recovery. the enzyme has a molecular weight of 29kda and appeared as a single band on native-page. the optimum ...201728634057
production of melanin by soil microbial isolate on fruit waste extract: two step optimization of key parameters.in this study, optimization of production parameters influencing melanin production in an economical fruit waste extract was attempted using a garden soil isolate (bacillus safensis). taguchi approach was adopted for screening of critical parameters and further optimization was done using a central composite design of response surface methodology (rsm). at optimum conditions (ph 6.84 and temp 30.7 °c), a significant yield of ∼6.96 mg/ml was observed. statistical analysis revealed that the experi ...201428626673
biodegradation of chlorpyrifos, malathion, and dimethoate by three strains of bacteria isolated from pesticide-polluted soils in sudan.this study was done to identify pesticide-biodegrading microorganisms and to characterize degradation rates. bacillus safensis strain fo-36b(t), bacillus subtilis subsp. inaquosorum strain kctc13429(t), and bacillus cereus strain atcc14579(t) were isolated from pesticide-polluted soil in sudan, separately incubated with each pesticide with periodic samples drawn for gc and gc-ms. pesticide biodegradation followed a biphasic model. α and β half-lives (days) of chlorpyrifos, malathion, and dimetho ...201627771954
metagenomic alkaline protease from mangrove sediment.functional screening of metagenomic libraries is an important tool for the discovery of new molecules. the metabolic diversity of microorganisms enables survival in harsh environments and is related to the production of enzymes. in this study, we identified a protease-producing clone from a metagenomic library derived from mangrove sediment. the protease was purified by ammonium sulphate precipitation and gel filtration chromatography, with a yield of 77.27% and a specific activity of 8.57 u μg( ...201728804942
root-induced changes of zn and pb dynamics in the rhizosphere of sunflower with different plant growth promoting treatments in a heavily contaminated soil.root induced changes are deemed to have an important role in the success of remediation techniques in contaminated soils. here, the effects of two nano-particles [sio2 and zeolite] with an application rate of 200mgkg(-1), and two bacteria [bacillus safensis fo-036b(t) and pseudomonas fluorescens p.f.169] in the rhizosphere of sunflower on zn and pb dynamics were studied in greenhouse conditions. the treatments reduced the exchangeable zn (from 13.68% to 30.82%) and pb (from 10.34% to 25.92%) in ...201728843530
isolation and genetic identification of spore-forming bacteria associated with concentrated-milk processing in nebraska.spore-forming bacteria are heat-resistant microorganisms capable of surviving and germinating in milk after pasteurization. they have been reported to affect the quality of dairy products by the production of enzymes (lipolytic and proteolytic) under low-temperature conditions in fluid milk, and have become a limiting factor for milk powder in reaching some selective markets. the objective of this research was to isolate and identify the population of spore-forming bacteria (psychrotrophic and t ...201727988120
molecular identification and safety of bacillus species involved in the fermentation of african oil beans (pentaclethra macrophylla benth) for production of ugba.molecular identification of bacillus spp. involved in the fermentation of african oil bean seeds for production of ugba, as well as ability of the bacillus spp. isolated to produce toxins, were investigated. forty-nine bacteria were isolated from ugba produced in different areas of south eastern nigeria and identified by phenotyping and sequencing of 16s rrna, gyrb and rpob genes. genotypic diversities at interspecies and intraspecies level of the isolates were screened by pcr amplification of t ...201323376783
phylogenetic and clonality analysis of bacillus pumilus isolates uncovered a highly heterogeneous population of different closely related species and clones.bacillus pumilus is a gram-positive bacterium with a wide range of attributed applications, namely as a plant growth promoting rhizobacteria (pgpr), animal, and human probiotic. however, a rare putative role in human diseases has been reported, namely in food poisoning or as anthrax-like cutaneous infectious agent. this species is difficult to distinguish from its closely related species on the basis of phenotypic or biochemical characteristics and 16s rrna gene sequences. in this study, the phy ...201425230950
microbial growth under supercritical co2.growth of microorganisms in environments containing co2 above its critical point is unexpected due to a combination of deleterious effects, including cytoplasmic acidification and membrane destabilization. thus, supercritical co2 (scco2) is generally regarded as a sterilizing agent. we report isolation of bacteria from three sites targeted for geologic carbon dioxide sequestration (gcs) that are capable of growth in pressurized bioreactors containing scco2. analysis of 16s rrna genes from scco2 ...201525681188
biodiversity of aerobic endospore-forming bacterial species occurring in yanyanku and ikpiru, fermented seeds of hibiscus sabdariffa used to produce food condiments in benin.yanyanku and ikpiru made by the fermentation of malcavene bean (hibiscus sabdariffa) are used as functional additives for parkia biglobosa seed fermentations in benin. a total of 355 aerobic endospore-forming bacteria (aefb) isolated from yanyanku and ikpiru produced in northern and southern benin were identified using phenotypic and genotypic methods, including gtg5-pcr, m13-pcr, 16s rrna, gyra and gyrb gene sequencing. generally, the same 5-6 species of the genus bacillus predominated: bacillu ...201323571124
bacillus invictae sp. nov., isolated from a health product.a gram-positive, rod-shaped, endospore-forming bacillus isolate, bi.(ffup1) (t), recovered in portugal from a health product was subjected to a polyphasic study and compared with the type strains of bacillus pumilus, bacillus safensis, bacillus altitudinis and bacillus xiamenensis, the phenotypically and genotypically most closely related species. acid production from cellobiose, d-glucose and d-mannose and absence of acid production from d-arabinose, erythritol, inositol, maltose, mannitol, raf ...201425171924
xylanases of bacillus spp. isolated from ruminant dung as potential accessory enzymes for agro-waste saccharification.polysaccharide hydrolase producing bacteria were isolated for biomass saccharification step in two-step bioethanol production. xylanolytic bacteria were found to be common in ruminant dung. seven bacillus dung isolates exhibited high xylanolytic activity; three of which were identified as bacillus safensis and four as bacillus altitudinis, based on 16s rdna and gyrb gene sequencing. interestingly, comparison of activity profiles for b. safensis m35 and b. altitudinis r31 and j208 crude xylanases ...201525645626
bacillus xiamenensis sp. nov., isolated from intestinal tract contents of a flathead mullet (mugil cephalus).a taxonomic study was carried out on strain hyc-10(t), which was isolated from the intestinal tract contents of a flathead mullet, mugil cephalus, captured from the sea off xiamen island, china. the bacterium was observed to be gram positive, oxidase and catalase positive, rod shaped, and motile by subpolar flagella. the bacterium was found to grow at salinities of 0-12 % and at temperatures of 8-45 °c. the isolate was found to hydrolyze aesculin and gelatin, but was unable to reduce nitrate to ...201424158533
application of a statistically enhanced, novel, organic solvent stable lipase from bacillus safensis dvl-43.this paper presents the molecular identification of a newly isolated bacterial strain producing a novel and organic solvent stable lipase, statistical optimization of fermentation medium, and its application in the synthesis of ethyl laurate. on the basis of nucleotide homology and phylogenetic analysis of 16s rdna sequence, the strain was identified as bacillus safensis dvl-43 (gen-bank accession number kc156603). optimization of fermentation medium using plackett-burman design and response sur ...201424534493
quantification of encapsulated bioburden in spacecraft polymer materials by cultivation-dependent and molecular methods.bioburden encapsulated in spacecraft polymers (such as adhesives and coatings) poses a potential risk to jeopardize scientific exploration of other celestial bodies. this is particularly critical for spacecraft components intended for hard landing. so far, it remained unclear if polymers are indeed a source of microbial contamination. in addition, data with respect to survival of microbes during the embedding/polymerization process are sparse. in this study we developed testing strategies to qua ...201424736730
genome sequence of salt-tolerant bacillus safensis strain vk, isolated from saline desert area of gujarat, india. 201424744343
identification of bacillus species occurring in kantong, an acid fermented seed condiment produced in ghana.kantong is a condiment produced in ghana by the spontaneous fermentation of kapok tree (ceiba pentandra) seeds with cassava flour as an additive. fermentation is over a 48h period followed by a drying and a kneading process. although lactic acid bacteria (lab) have previously been identified other micro-organisms may also be involved in the fermentation process. in this study we examined the occurrence of aerobic endospore-forming bacteria (aeb) in raw materials, during fermentation and in the f ...201424747716
draft genome sequence of bacillus safensis jpl-merta-8-2, isolated from a mars-bound spacecraft.here, we present the draft genome of bacillus safensis jpl-merta-8-2, a strain found in a spacecraft assembly cleanroom before launch of the mars exploration rovers. the assembly contains 3,671,133 bp in 14 contigs.201526586895
growth of 48 built environment bacterial isolates on board the international space station (iss).background. while significant attention has been paid to the potential risk of pathogenic microbes aboard crewed spacecraft, the non-pathogenic microbes in these habitats have received less consideration. preliminary work has demonstrated that the interior of the international space station (iss) has a microbial community resembling those of built environments on earth. here we report the results of sending 48 bacterial strains, collected from built environments on earth, for a growth experiment ...201627019789
genome mining of lipolytic exoenzymes from bacillus safensis s9 and pseudomonas alcaliphila ed1 isolated from a dairy wastewater lagoon.dairy production plants produce highly polluted wastewaters rich in organic molecules such as lactose, proteins and fats. fats generally lead to low overall performance of the treatment system. in this study, a wastewater dairy lagoon was used as microbial source and different screening strategies were conducted to select 58 lipolytic microorganisms. exoenzymes and rapd analyses revealed genetic and phenotypic diversity among isolates. bacillus safensis, pseudomonas alcaliphila and the potential ...201627270463
biochemical, molecular, and transcriptional highlights of the biosynthesis of an effective biosurfactant produced by bacillus safensis pha3, a petroleum-dwelling bacteria.petroleum crude oil (pco)-dwelling microorganisms have exceptional biological capabilities to tolerate the toxicity of petroleum contaminants and are therefore promising emulsifier and/or degraders of pco. this study describes a set of pco-inhabiting bacterial species, one of which, identified as bacillus safensis pha3, produces an efficient biosurfactant which was characterized as a glycolipid. fourier transform infrared spectrometer, nuclear magnetic resonance, thin layer chromatography, hplc, ...201728179901
genomic and chemical insights into biosurfactant production by the mangrove-derived strain bacillus safensis ccma-560.many bacillus species can produce biosurfactant, although most of the studies on lipopeptide production by this genus have been focused on bacillus subtilis. surfactants are broadly used in pharmaceutical, food and petroleum industry, and biological surfactant shows some advantages over the chemical surfactants, such as less toxicity, production from renewable, cheaper feedstocks and development of novel recombinant hyperproducer strains. this study is aimed to unveil the biosurfactant metabolic ...201525586584
identification of oxidoreductases from the petroleum bacillus safensis strain.a gram-positive bacterium, denominated cfa-06, was isolated from brazilian petroleum in the campos basin and is responsible for the degradation of aromatic compounds and petroleum aromatic fractions. the cfa-06 strain was identified as bacillus safensis using the 16s rrna and gyrase b sequence. enzymatic assays revealed the presence of two oxidoreductases: a catalase and a new oxidoreductase. the oxidoreductases were enzymatically digested and analyzed via esi-ltq-orbitrap mass spectrometry. the ...201528352585
bacteria present in comadia redtenbacheri larvae (lepidoptera: cossidae).the external and internal culturable bacterial community present in the larvae of comadia redtenbacheri hammerschmidt, an edible insect, was studied. characterization of the isolates determined the existence of 18 morphotypes and phylogenetic analysis of the 16s rrna gene revealed the existence of paenibacillus sp., bacillus safensis, pseudomonas sp., bacillus pseudomycoides, corynebacterium variabile, enterococcus sp., gordonia sp., acinetobacter calcoaceticus, arthrobacter sp., micrococcus sp. ...201526336239
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