genetic and epidemiological characterization of stretch lagoon orbivirus, a novel orbivirus isolated from culex and aedes mosquitoes in northern australia. | stretch lagoon orbivirus (slov) was isolated in 2002 from pooled culex annulirostris mosquitoes collected at stretch lagoon, near the wolfe creek national park in the kimberley region of western australia. conventional serological tests were unable to identify the isolate, and electron microscopy indicated a virus of the genus orbivirus, family reoviridae. here, a cdna subtraction method was used to obtain approximately one-third of the viral genome, and further sequencing was performed to compl ... | 2009 | 19282430 |
Umatilla virus genome sequencing and phylogenetic analysis: identification of stretch lagoon orbivirus as a new member of the Umatilla virus species. | The genus Orbivirus, family Reoviridae, includes 22 species of viruses with genomes composed of ten segments of linear dsRNA that are transmitted between their vertebrate hosts by insects or ticks, or with no identified vectors. Full-genome sequence data are available for representative isolates of the insect borne mammalian orbiviruses (including bluetongue virus), as well as a tick borne avian orbivirus (Great Island virus). However, no sequence data are as yet available for the mosquito borne ... | 2011 | 21897849 |
enhanced arbovirus surveillance with deep sequencing: identification of novel rhabdoviruses and bunyaviruses in australian mosquitoes. | viral metagenomics characterizes known and identifies unknown viruses based on sequence similarities to any previously sequenced viral genomes. a metagenomics approach was used to identify virus sequences in australian mosquitoes causing cytopathic effects in inoculated mammalian cell cultures. sequence comparisons revealed strains of liao ning virus (reovirus, seadornavirus), previously detected only in china, livestock-infecting stretch lagoon virus (reovirus, orbivirus), two novel dimarhabdov ... | 2013 | 24314645 |
first isolation and characterization of a mosquito-borne orbivirus belonging to the species umatilla virus in east asia. | an orbivirus was isolated from a sample from the ornithophilic mosquito culex sasai in japan. the virus, designated koyama hill virus (khv), replicated to high titer in a mosquito cell line and to a low titer in an avian cell line, but the release of progeny viruses was not observed in mammalian cell lines inoculated with khv. electron microscopic examination of khv-infected mosquito cells showed approximately 70-nm virus particles and viral tubules typical of members of the genus orbivirus, fam ... | 2014 | 24906523 |