| host range and complete genome sequence of cucurbit chlorotic yellows virus, a new member of the genus crinivirus. | cucurbit chlorotic yellows virus (ccyv) causes chlorotic yellows on cucumber (cucumis sativus) and melon (cucumis melo) and is transmitted by bemisia tabaci biotype b and q whiteflies. to characterize the host range of ccyv, 21 cucurbitaceous and 12 other plant species were inoculated using whitefly vectors. all tested cucumis spp. except cucumis anguria and cucumis zeyheri were systemically infected with ccyv, although infection rates varied among species. citrullus lanatus, cucurbita pepo, and ... | 2010 | 20465411 |
| infectious clones of the crinivirus cucurbit chlorotic yellows virus are competent for plant systemic infection and vector transmission. | cucurbit chlorotic yellows virus (ccyv), a recently identified bipartite crinivirus, causes economic losses in cucurbit plants. ccyv is naturally transmitted only by whitefly bemisia tabaci. here we constructed full-length cdna clones of ccyv (rna1 and rna2) fused to the t7 rna polymerase promoter and the cauliflower mosaic virus 35s promoter. ccyv replicated and accumulated efficiently in cucumis sativus protoplasts transfected with in vitro transcripts. without rna2, rna1 replicated efficientl ... | 2016 | 26982585 |
| whitefly-transmitted criniviruses of cucurbits: current status and future prospects. | in the past decade, crinviruses have gained interest due to their rapid widespread and destructive nature for cucurbit cultivation. several members of the genus crinivirus are considered emerging viruses. currently, four criniviruses: beet pseudo-yellows virus, cucurbit chlorotic yellows virus, cucurbit yellow stunting disorder virus and lettuce infectious yellows virus have been reported to infect field- or greenhouse- grown cucurbits. apart from their cucurbit hosts, criniviruses infect other ... | 2014 | 24426308 |
| detection and quantitation of two cucurbit criniviruses in mixed infection by real-time rt-pcr. | cucurbit chlorotic yellows virus (ccyv) and cucurbit yellow stunting disorder virus (cysdv) are whitefly-transmitted criniviruses infecting cucurbit crops inducing similar symptoms. single and multiplex rt-pcr protocols were developed and evaluated on cucurbit samples collected from commercial greenhouses. primers and probes were designed from the highly conserved heat shock protein 70 homolog (hsp70h) gene. conventional rt-pcr and multiplex rt-pcr assays showed high specificity and suitability ... | 2013 | 23810855 |
| two proteins of cucurbit chlorotic yellows virus, p59 and p9, are self-interacting. | the yeast two-hybrid (y2h) assay, a powerful tool for identifying protein-protein interactions, has been widely used to study viral protein interactions and to elucidate the functions of viral proteins. in this study, cucurbit chlorotic yellows virus-encoded proteins were investigated by y2h assays in all possible pairwise combinations, and the self-interactions of p59 and p9 were detected. the interacting domains of p59 and p9 were identified using vectors carrying an activation domain fused to ... | 2015 | 26001989 |
| one-step reverse transcription loop mediated isothermal amplification assay for sensitive and rapid detection of cucurbit chlorotic yellows virus. | a reverse transcription loop-mediated isothermal amplification (rt-lamp) assay was developed for the detection of cucurbit chlorotic yellows virus (ccyv). in this procedure, a set of four primers matching a total of six sequences in the coat protein gene region of ccyv was synthesized for the rt-lamp assay using total rna extracted from ccyv-infected melon leaf tissues, and the optimum reaction temperature and assay time were determined. the sensitivity assay showed that the virus was detectable ... | 2014 | 24056260 |
| a semipersistent plant virus differentially manipulates feeding behaviors of different sexes and biotypes of its whitefly vector. | it is known that plant viruses can change the performance of their vectors. however, there have been no reports on whether or how a semipersistent plant virus manipulates the feeding behaviors of its whitefly vectors. cucurbit chlorotic yellows virus (ccyv) (genus crinivirus, family closteroviridae) is an emergent plant virus in many asian countries and is transmitted specifically by b and q biotypes of tobacco whitefly, bemisia tabaci (gennadius), in a semipersistent manner. in the present stud ... | 2017 | 28098749 |
| direct evidence for the semipersistent transmission of cucurbit chlorotic yellows virus by a whitefly vector. | cucurbit chlorotic yellows virus (ccyv) (genus crinivirus, family closteroviridae) is an emerging plant virus, and is now spreading and causing severe economic losses to cucurbit crops in many asian countries. ccyv is believed to be transmitted specifically by the sweetpotato whitefly, bemisia tabaci, in a semipersistent manner. in the present study, we provide direct evidence for the semipersistent transmission of ccyv by mediterranean (med) cryptic species of b. tabaci complex. we investigated ... | 2016 | 27812032 |
| detection of cucurbit chlorotic yellows virus from bemisia tabaci captured on sticky traps using reverse transcription loop-mediated isothermal amplification (rt-lamp) and simple template preparation. | cucurbit chlorotic yellows virus (ccyv) of the genus crinivirus within the family closteroviridae is an emerging infectious agent of cucurbits leading to severe disease and significant economic losses. effective detection and identification methods for this virus are urgently required. in this study, a reverse transcription loop-mediated isothermal amplification (rt-lamp) assay was developed to detect ccyv from its vector bemisia tabaci. lamp primer sets to detect ccyv were evaluated for their s ... | 2015 | 25912723 |
| induction of antiviral responses by acibenzolar-s-methyl against cucurbit chlorotic yellows virus in melon. | cucurbit chlorotic yellows virus (ccyv) (family closteroviridae, genus crinivirus) is an emerging virus which causes severe diseases on melon (cucumis melo) plants. ccyv-infected melon plants display yellowing, mottling, chlorosis, or chlorotic spots on leaves. to develop a new control strategy, the potential for 1,2,3-benzothiadiazole-7-thiocarboxylic acid-s-methyl-ester (asm) to suppress ccyv infection was evaluated. asm treatment on melon plants greatly increased the expression levels of path ... | 2013 | 23927428 |
| transcriptome analysis of cucumis sativus infected by cucurbit chlorotic yellows virus. | cucurbit chlorotic yellows virus (ccyv) is a recently reported bipartite crinivirus that causes chlorotic leaf spots and yellowing symptoms on the leaves of cucurbit plants. the virus-host interaction of ccyv remains to be elucidated, and the influence of criniviruses on the host gene transcriptome requires analysis. | 2017 | 28148297 |
| detection and epidemic dynamic of tocv and ccyv with bemisia tabaci and weed in hainan of china. | in recent years, two of the crinivirus, tomato chlorosis virus (tocv) and cucurbit chlorotic yellows virus (ccyv) have gained increasing attention due to their rapid spread and devastating impacts on vegetable production worldwide. both of these viruses are transmitted by the sweet potato whitefly, bemisia tabaci (gennadius), in a semi-persistent manner. up to now, there is still lack of report in hainan, the south of china. | 2017 | 28870255 |