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propionibacterium acidifaciens sp. nov., isolated from the human mouth.three strains of anaerobic, pleomorphic, gram-positive-staining bacilli, which were isolated from human carious dentine, were subjected to a comprehensive range of phenotypic and genotypic tests and were found to comprise a homogeneous group. the strains were saccharolytic and produced acetic and propionic acids in large amounts, and succinic acid in moderate amounts, as the end products of fermentation. 16s rrna gene and rpob protein sequence analyses revealed that the strains constituted a nov ...200919625418
extensive description and comparison of human supra-gingival microbiome in root caries and health.knowledge of the polymicrobial etiology of root caries is limited. to conduct a comprehensive research study on root caries, we utilized 454-pyrosequencing of 16s rrna gene libraries and quantitative pcr to compare supra-gingival bacterial communities from healthy sites and carious sites of 21 patients with root caries (patient-controls and patient-cases) and the sites of 21 healthy individuals (healthy-controls) from two nursing homes. healthy-controls and patient-cases showed no significant di ...201525658087
antibiotic resistance and capacity for biofilm formation of different bacteria isolated from endodontic infections associated with root-filled teeth.to date, a variety of microbial species have been isolated from endodontic infections. however, endodontic clinical bacterial isolates have not been sufficiently characterized with regard to their capacity for antibiotic resistance and biofilm formation. in this study, antibiotic resistance and biofilm formation of 47 different aerobic and anaerobic bacterial isolates, belonging to 32 different species previously isolated from infected filled root canals, were studied.201424461408
bacterial biofilm composition in caries and caries-free subjects.certain major pathogens such as streptococcus mutans, lactobacillus spp. and others have been reported to be involved in caries initiation and progression. yet, in addition to those leading pathogens, microbial communities seem to be much more diverse and individually differing. the aim of this study, therefore, was to analyze the bacterial composition of carious dentin and the plaque of caries-free patients by using a custom-made, real-time quantitative polymerase chain reaction assay (rq-pcr).201323147531
detection of signature volatiles for cariogenic microorganisms.the development of a breath test by the identification of volatile organic compounds (vocs) emitted by cariogenic bacteria is a promising approach for caries risk assessment and early caries detection. the aim of the present study was to investigate the volatile profiles of three major cariogenic bacteria and to assess whether the obtained signatures were species-specific. therefore, the headspaces above cultures of streptococcus mutans, lactobacillus salivarius and propionibacterium acidifacien ...201626610336
comparison of the in vivo antimicrobial effectiveness of sodium hypochlorite and chlorhexidine used as root canal irrigants: a molecular microbiology study.the purpose of this clinical study was to compare the antimicrobial effects of 2.5% sodium hypochlorite (naocl) and 0.12% chlorhexidine digluconate (chx) when used as irrigants during treatment of teeth with apical periodontitis.201121238793
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