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toxicity of fenitrothion, an organophosphorus pesticide, against summer population of sunn pest, eurygaster integriceps put. (hemiptera: scutelleridae).the sunn pest are a group of insect representing several genera of the shield bug (scutelleridae) and stink bug (pentatomidae) families, with the species eurygaster integriceps being the most economically important. the sunn pest is a major pest of wheat and barley in central and western asia and it accounts for annual losses of 20-30% for barley and up to 100% for wheat. in the recent decades problems have intensified largely due to change in farming practices. thus this pest threatens food sec ...200516628916
a amylase activity of nymphal stages of sunn pest, eurygaster integriceps puton (hemiptera: scutelleridae).wheat production in iran has changed substantially over the past one or two decades with development of higher-yielding cultivars and improved methods of planting. sunn pest, eurygaster integriceps (heteroptera: pentatomidae), is the most important cereal pest in iran. sunn pest like other insect pests of wheat lives on a polysaccharide-rich diet and depends to a large extent on effectiveness of their alpha-amylases for survival. alpha-amylase (1-4-alpha-d-glucan glucanohydrolase) hydrolyses sta ...200516628929
effect of plant a-amylase inhibitors on sunn pest, eurygaster integriceps puton (hemiptera: scutelleridae), alpha-amylase activity.plant-insect interaction is a dynamic system, subjected to continual variation and change. in order to reduce insect attack, plants developed different defence mechanisms including chemical and physical barriers such as the induction of defensive proteins, volatiles that attract predators of the insect herbivores and secondary metabolites. proteinaceous inhibitors of alpha-amylase and proteases are widely distributed in cereals, legumes and some other plants. because of the possible importance o ...200516628930
oil formulation of entomopathogenic fungus, beauveria bassian, against sunn pest, eurygaster integriceps puton (heteroptera: scutelleridae).entomopathogenic fungi with approximately 1000 known species are key regulatory factors in insect pest population. also, these agents infect insects by direct penetration of the cuticle and thus provide the only practical means of microbial control of insects that feed on plant or animal juices as well as for the many coleopterans and orthopteran pest which have very few known viral or bacterial diseases. beauveria bassiana is a widespread entomopathogenic fungi that infect many insect species. ...200617385512
comparison of pathogenicity of two isolates of the entomopathogenic fungus metarhizium anisopliaeon adult sunn pest (eurygaster integriceps puton.).sunn pest, eurygaster integriceps puton (heteroptera: scutelleridae), is one of the major wheat pest in iran and neighboring countries. in order to control this pest, every year hundred of thousands hectares of wheat crop are sprayed with synthetic pesticides in iran. biocontrol, the use of living organisms as pest control agents, became an important alternative to the use of chemical pesticides and, therefore, a potential means of reducing pesticide use and its undesirable effects on human heal ...200617385522
molecular characterization of isolates of beauveria bassiana obtained from overwintering and summer populations of sunn pest (eurygaster integriceps).to examine whether isolates of the entomopathogenic fungus beauveria bassiana are more closely associated to their summer hosts compared with overwintering hosts, with recently developed molecular tools based on mitochondrial regions.200818290810
effects of artemisia annua l. (asteracea) on the digestive enzymatic profiles and the cellular immune reactions of the sunn pest, eurygaster integriceps (heteroptera: scutellaridae), against beauveria bassiana.plant extracts are currently studied more and more because of the possibility of their usage in plant protection. many of the natural plant compounds which are used in the control of pests are known to affect the digestion and immune functions of insects. in this study, effects of artemisia annua extract on the digestive enzymatic profiles and the cellular immune reactions of eurygaster integriceps were investigated to reach a better understanding of its role in the control of this pest as the m ...201019519976
beauveria bassiana characterization and efficacy vs. sunn pest, eurygaster integriceps puton (hemiptera:scutelleridae).the objectives of this study were to evaluate the efficacy of sunn pest entomopathogenic fungi collected from wheat fields and overwintering sites in syria and characterize them under different temperature regimes. eleven isolates of beauveria bassiana (bals.) vuil. were included in this study: five were isolated from overwintered adults of sunn pest, eurygaster integriceps puton and six from nymphs and new adults in wheat fields. growth rate and conidial production were tested at 15, 20, 25, 30 ...201021313877
hexamermis eurygasteri n. sp. (nematoda: mermithidae) parasitising the sunn pest eurygaster integriceps puton (hemiptera: scutelleridae) in turkey.a new species of mermithid nematode, hexamermis eurygasteri n. sp. (nematoda: mermithidae) is described as a parasite of the sunn pest eurygaster integriceps puton (hemiptera: scutelleridae) in turkey. the combination of the following characters separate h. eurygasteri from other members of hexamermis steiner, 1924, as defined by artyukhovsky (1990) and kaiser (1991): amphidial pouch integrated into lateral cephalic papillae; amphidial openings minute; well-developed cuticular vulval cone; small ...201121643896
cellular immune reactions of the sunn pest, eurygaster integriceps, to the entomopathogenic fungus, beauveria bassiana and its secondary metabolites.in this study, five morphological types of circulating hemocytes were recognized in the hemolymph of the adult sunn pest, eurygaster integriceps puton (hemiptera: scutelleridae), namely prohemocytes, plasmatocytes, granulocytes, adipohemocytes, and oenocytoids. the effects of the secondary metabolites of the entomopathogenic fungus beauveria bassiana on cellular immune defenses of eurygaster integriceps were investigated. the results showed that the fungal secondary metabolites inhibited phagocy ...201122233481
[the sensory neuropile of the nerve chain of eurygaster integriceps p].structure of the sensory neuropil of the abdominal nervous chain has been studied in the eurygaster integriceps p. by means of methylene blue and paraldehyde fuchsin with phloxin staining. the sensory neuropil consists of three parts: 1) main sensory neuropil, including terminal, t-shaped, antero- and posterconnective fibers; 2) lateral sensory neuropils of the extremities, that in the insect studied, as in the dragon-fly larva, have the form of the overturned eight; 3) system of thick t-shaped ...20082423058
cloning, expression, sequence analysis and homology modeling of the prolyl endoprotease from eurygaster integriceps puton.eurygaster integriceps puton, commonly known as sunn pest, is a major pest of wheat in northern africa, the middle east and eastern europe. this insect injects a prolyl endoprotease into the wheat, destroying the gluten. the purpose of this study was to clone the full length cdna of the sunn pest prolyl endoprotease (sppep) for expression in e. coli and to compare the amino acid sequence of the enzyme to other known peps in both phylogeny and potential tertiary structure. sequence analysis shows ...201426462938
role of symbiotic bacteria in the growth and development of the sunn pest, eurygaster integriceps.the sunn pest, eurygaster integriceps puton (hemiptera: scutelleridae), is the most important pest of wheat and barley in wide areas of the world. different aspects of the insect's life history have been studied, but to date nothing is known about their microbial symbionts. here, the contribution of symbiotic bacteria to the fitness of the bug was investigated by combining two different approaches to manipulate the host's microbial community: the supplementation of antibiotics into the insects' ...201324205987
pathogenicity of the entomopathogenic fungus metarhizium anisopliae var. major on different stages of the sunn pest eurygaster integriceps.the sunn pest, eurygaster integriceps puton (hemiptera: scutelleridae), is the most important insect pest of wheat and barley. the population management of this pest is of major concern to wheat producers. one of the potential control strategies is to use entomopathogenic fungi. this study evaluates the pathogenicity of the fungus metarhizium anisopliae var. major (metchnikoff) sorokin (hypocreales: clavicipitaceae) on the sunn pest, e. integriceps. five concentrations of the fungus were utilize ...201324766585
[production and properties of recombinant glutenin-cleaving proteinases from eurygaster integriceps put].cdnas coding for a mature form of glutenin-cleaving trypsin-like proteinase (referred to as glutenin-hydrolyzing proteinase 3 or ghp3) from the insect pest-eurygaster integriceps put. and a zymogen of this proteinase containing a signal peptide required for protein secretion were cloned into vectors ppic9 and ppic3.5, respectively. the constructs were used for protein expression in cells of the methylotrophic yeast pichia pastoris. the recombinant protein corresponding to the mature form of the ...201625707103
molecular identification of cysteine and trypsin protease, effect of different hosts on protease expression, and rnai mediated silencing of cysteine protease gene in the sunn pest.sunn pest, eurygaster integriceps, is a serious pest of cereals in the wide area of the globe from near and middle east to east and south europe and north africa. this study described for the first time, identification of e. integriceps trypsin serine protease and cathepsin-l cysteine, transcripts involved in digestion, which might serve as targets for pest control management. a total of 478 and 500 base pair long putative trypsin and cysteine gene sequences were characterized and named tryp and ...026609789
qtl mapping identifies a major locus for resistance in wheat to sunn pest (eurygaster integriceps) feeding at the vegetative growth stage.this research provides the first report of a major locus controlling wheat resistance to sunn pest. it developed and validated snp markers that will be useful for marker-assisted selection. sunn pest (eurygaster integriceps puton) is the most destructive insect pest of bread wheat and durum wheat in west and central asia and east europe. breeding for resistance at the vegetative stage of growth is vital in reducing the damage caused by overwintered adult populations that feed on shoot and leaves ...201727744491
analysis of the spatio-temporal distribution of eurygaster integriceps (hemiptera: scutelleridae) by using spatial analysis by distance indices and geostatistics.eurygaster integriceps puton (hemiptera: scutelleridae) is the most serious insect pest of wheat (triticum aestivum l.) and barley (hordeum vulgare l.) in iran. in this study, spatio-temporal distribution of this pest was determined in wheat by using spatial analysis by distance indices (sadie) and geostatistics. global positioning and geographic information systems were used for spatial sampling and mapping the distribution of this insect. the study was conducted for three growing seasons in gh ...201122251736
[receptors of the head, thoracic and abdominal segments in the shield bug eurygaster integriceps]. 201711190147
dna barcoding and morphological analysis for rapid identification of most economically important crop-infesting sunn pests belonging to eurygaster laporte, 1833 (hemiptera, scutelleridae).the genus eurygaster laporte, 1833 includes ten species five of which inhabit the european part of russia. the harmful species of the genus is e. integriceps. eurygaster species identification based on the morphological traits is very difficult, while that of the species at the egg or larval stages is extremely difficult or impossible. eurygaster integriceps, e. maura, and e. testudinaria differ only slightly between each other morphologically, e. maura and e. testudinaria being almost indiscern ...201729118620
modelling the current and potential future distributions of the sunn pest eurygaster integriceps (hemiptera: scutelleridae) using climex.the sunn pest, eurygaster integriceps (hemiptera: scutelleridae), is an economically significant pest throughout western asia and eastern europe. this study was conducted to examine the possible risk posed by the influence of climate change on its spread. climex software was used to model its current global distribution. future invasion potential was investigated using two global climate models (gcms), csiro-mk3.0 (cs) and miroc-h (mr), under a1b and a2 emission scenarios for 2030, 2070 and 2100 ...201626833543
reciprocal crosses between two populations of trissolcus vassilievi (mayr) (hymenoptera: scelionidae) reveal maternal effects on thermal phenotypes.the egg parasitoid trissolcus vassilievi (mayr) (hymenoptera: scelionidae) is a significant natural enemy of the sunn pest, eurygaster integriceps puton (hemiptera: scutelleridae), the most important pest of wheat in iran. this study examined the developmental time and egg-to-adult survival of two geographically separate populations of t. vassilievi on two corresponding host populations at five constant temperatures ranging from 15.0 to 35.0 ± 1°c. no wasps of either population emerged at 15.0°c ...201525809416
salivary digestive enzymes of the wheat bug, eurygaster integriceps (insecta: hemiptera: scutelleridae).the digestive enzymes from salivary gland complexes (sgc) of eurygaster integriceps, and their response to starvation and feeding were studied. moreover, digestive amylases were partially purified and characterized by ammonium sulfate precipitation and gel filtration chromatography. the sgc are composed of two sections, the principal glands and accessory glands. the principal glands are further divided into the anterior lobes and posterior lobes. the sgc main enzyme was α-amylase, which hydrolyz ...201424961557
proteome analysis of gut and salivary gland proteins of fifth-instar nymph and adults of the sunn pest, eurygaster integriceps.in the digestive system of the sunn pest, eurygaster integriceps puton (hemiptera: scutelleridae), the salivary gland has a key role in extra oral digestion and the gut is the main site for digestion of food. in this study, proteomics was used to study the role of proteins involved in digestion. the amount of feeding on wheat grain by adult insects increased by comparison to fifth-instar nymphs. proteins of the gut and salivary gland in adults and fifth-instar nymphs were analyzed 1 day after fe ...201222951809
the sunn pest, eurygaster integriceps puton (hemiptera: scutelleridae) digestive tract: histology, ultrastructure and its physiological significance.the sunn pest, eurygaster integriceps put. (hemiptera: scutelleridae), is a key pest of wheat in the middle east and some other areas which causes severe qualitative and quantitative damage. the objective of the current work is to describe the morphology of the midgut of e. integriceps adult. microscopic studies revealed that foregut consists of oral cavity, pharynx and oesophagus likely other phytophagous hemiptera. in the midgut, four anatomical regions could be identified: the first ventricul ...201222227010
secretion and formation of perimicrovillar membrane in the digestive system of the sunn pest, eurygaster integriceps (hemiptera: scutelleridae) in response to feeding.in this study, development of perimicrovillar membrane (pmm) from midgut cells of starved and fed eurygaster integriceps (hemiptera: scutelleridae) was studied. three different approaches, including scanning electron microscopy (sem), transmission electron microscopy (tem), marker enzymes of the pmms (α-glucosidase), perimicrovillar space (aminopeptidase), and microvillar membranes (β-glucosidase) were used. activities of these enzymes were remarkably low in the starved insects. moreover, micros ...201122105665
effects of serine protease inhibitors on growth and development and digestive serine proteinases of the sunn pest, eurygaster integriceps.in the current study the effects of serine proteinase inhibitors (tlck, tpck, sbti, and a combination of sbti and tpck) with concentrations of 1% and 4% of dietary protein in artificial diets were tested against growth of the sunn pest, eurygaster integriceps puton (hemiptera: scutelleridae), development, and its gut serine proteinase targets. analysis of variance indicated that protease inhibitors affected nymphal development time, adult weight, and survival. mean development time of third inst ...201121867440
characterization of a glutenin-specific serine proteinase of sunn bug eurygaster integricepts put.glutenin hydrolyzing proteinases (ghps) have been purified, by affinity chromatography, from wheat seeds damaged by the sunn bug eurygaster integriceps (hemiptera, scutelleridae). a 28 kda protein was partially sequenced by mass spectrometry and edman degradation which showed homology to serine proteases from various insects. three full length clones were obtained from cdna isolated from sunn bug salivary glands using degenerate pcr based on the sequences obtained. the cleavage site of the prote ...201121323348
inhibition of sunn pest, eurygaster integriceps, α-amylases by α-amylase inhibitors (t-αai) from triticale.the effect of triticale α-amylases inhibitors on starch hydrolysis catalyzed by the sunn pest, eurygaster integriceps puton (hemiptera: scutelleridae) midgut amylases was examined. biochemical studgawies showed that inhibitors from triticale (a hybrid of wheat and rye) had inhibitiory effects on e. integriceps α-amylases. the effects of the triticale α-amylase inhibitor (t-αai) on α-amylase of e. integriceps showed a dose dependent manner of inhibition, e.g. less inhibition of enzyme activity (a ...201021062146
purification and characterization of phenoloxidase from the hemocytes of eurygaster integriceps (hemiptera: scutelleridae).insects protect themselves from microbial invaders by two main immune activities, namely the cellular and humoral reactions. phenoloxidases are oxidative enzymes that have an important role in both cell-mediated and humoral immunity. in this study, the purification and biochemical characterization of a phenoloxidase from the hemocytes of the sunn pest eurygaster integriceps puton (hemiptera: scutelleridae) were carried out. after the final purification step, the enzyme was purified 7.31-fold wit ...201120970518
life history parameters of the sunn pest, eurygaster integriceps, held at four constant temperatures.the sunn-pest, eurygaster integriceps puton (heteroptera: scutelleridae), is the most important insect pest of wheat and barley in iran. a demographic study was carried out in order to determine the effect of temperature on life history parameters of the pest. life tables were constructed at four constant temperatures: 22, 25, 27, and 30 ± 1° c using mahdavi wheat kernels as food. finite and intrinsic rates of population increase, gross and net reproductive rates, intrinsic rates of birth and de ...201020874393
life tables for sunn pest, eurygaster integriceps (heteroptera: scutelleridae) in northern iran.life table studies of sunn pest were carried out in varamin, iran, from 1998-2001 in order to determine stage-specific mortalities and the impact of specific natural enemies on population dynamics. populations were sampled 2-3 times weekly in agricultural fields during the growing season and monthly during the period of dormancy at resting sites in nearby mountains some 30 km away from cereal fields. adults spend a period of 9-10 months in diapause and suffered overcompensatory, density-dependen ...201120569519
characterization of a prolyl endoprotease from eurygaster integriceps puton (sunn pest) infested wheat.sunn pest, eurygaster integriceps, puton, infested and uninfested wheat seeds were obtained from the international center for agriculture research in the dry areas (icarda), aleppo, syria, with the primary objective to identify the type of enzyme deposited by the sunn pest on the wheat responsible for the gluten degradation. enzyme levels were extremely low due to the enzyme being secreted by the insect in localized areas on the seed. only extract from the infested wheat contained glutenase acti ...201020568295
digestive proteolytic activity in the sunn pest, eurygaster integriceps.the sunn pest, eurygaster integriceps puton (heteroptera: scutelleridae), is one of the most important pests of wheat and causes considerable damage to this valuable crop annually. digestive proteinase activity of adult insects was investigated using general and specific substrates and inhibitors. proteolytic activity was low when the common conventional substrates, azoalbumin, azocasein and hemoglobin were used to assay salivary glands and midguts. using the fluorescent casein substrate (bodipy ...200920053125
subcellular fractionation of midgut cells of the sunn pest eurygaster integriceps (hemiptera: scutelleridae): enzyme markers of microvillar and perimicrovillar membranes.the subcellular distributions of six digestive and non-digestive enzymes (alpha-glucosidase, beta-glucosidase, alkaline phosphatase, acid phosphatase, aminopeptidase and lactate dehydrogenase) of eurygaster integriceps have been studied. the subcellular distributions of acid phosphatase and alpha-glucosidase are similar and the gradient ultracentrifugation profiles of these two enzymes overlap. two partially membrane-bound enzymes, alkaline phosphatase and beta-glucosidase have similar distribut ...201020035764
strategy of freeze tolerance in adult of sunn pest bug (eurygaster integriceps put.).to investigate cold hardiness of eurygaster integriceps put, super cooling points of whole bodies during december to march 2006-2007 were measured on samples collected from altitude of ghara-aghaj varamin-iran. in addition, the lower lethal temperature (llt) was determined for adult insect. the super cooling points were observed about -5 in cold months. since the minimum temperature under natural condition is reached to-7 degreesc or lower in january and llt99 (-21.65 degrees c), --11 degreesc l ...200718399476
esterase activity in summer population of sunn pest, eurygaster integriceps put. (hemiptera: scutelleridae).a key constraint on increasing wheat production in iran and some neighbouring countries is sunn pest which cause severe damage to vegetative growth stage of plant in the early season. it also feeds on wheat grain in late growth stage of plants thus damaged wheat grains loose their bakery properties. because of injecting protease enzymes into the grain during feeding, enzymes degrade gluten proteins and cause rapid relaxation of dough which results in the production of bread with poor volume and ...200516628931
lethal and sublethal effects of fenitrothion and deltamethrin residues on the egg parasitoid trissolcus grandis (hymenoptera: scelionidae).effects of fenitrothion and deltamethrin, the most commonly used insecticides in iran for controlling eurygaster integriceps puton (heteroptera: scutelleridae), in wheat and barley were assessed on adults and preimaginal stages of egg parasitoid trissolcus grandis thompson (hymenoptera: scelionidae). adult parasitoids exposed to field recommended concentrations of the insecticides suffered 100% mortality within 24 h. lc50 values of fenitrothion and deltamethrin for t. grandis were 8.1 and 3.9 mi ...200515765663
phospholipid and triacylglycerol fatty acid composition of major life stages of sunn pest, eurygaster integriceps (heteroptera: scutelleridae).phospholipid and triacylglycerol fatty acid compositions of whole animals from all life stages of eurygaster integriceps, including eggs, nymphs, pre-diapausing adults and diapausing adults, were determined. the fatty acid composition of total lipids of their food, wheat, was also determined. the major components of the insects and their food were the expected c16 and c18 saturated and unsaturated fatty acids. since fatty acid compositions of third-stadium nymphs were not similar to the profiles ...200212031463
[distribution of sensory fibers of the extremities in the neuropile of the abdominal chain of eurygaster integriceps].sensory neuropile of the extremities has been studied in series sections and total preparations from e. integriceps. extremity nuclei are fairly well distinguished from the rest neuropile. only terminal sensitive fibers enter the nucleus through the sensory root of the nerve. the main bulk of fibers is divided into two branches. with respect to the branching pattern, terminal fibers of the insect studied are similar to typical fibers of the sensory neuropile in the dragonfly, locust, and cricket ...20067405448
[effect of juvenile hormone analogs on morphogenetic processes in late embryogenesis of eurygaster integriceps]. 2017988684
[activation of diapausing shield bugs (eurygaster integriceps put.) using juvenile hormone analogs]. 20175043297
[wing removal as a factor stimulating egg maturation in insects (using eurygaster integriceps put. as an example)]. 20174325645
[role of metabolic water in the cleidoic eggs of insects (based on the example of the shellbug, eurygaster integriceps put.)]. 20175538516
[structure of the wall of the aorta, a specific neurohemal organ, in eurygaster integriceps put. (heteroptera, scutelleridae)]. 19705515438
[formation and activity of the neurosecretory system of the protocerebrum in the course of embryogenesis in eurygaster integriceps put]. 19675623433
[the differentiation of hemocytes in the embryogenesis of the turtle (eurygaster integriceps put.)]. 20035623202
[telenomus sokolovi mayr (hymenoptera, scelionidae) as a secondary parasite of the eggs of eurygaster integriceps put]. 19665998870
[application of the labelling method in the study of resistance of eurygaster integriceps to two organic phosphorus insecticides and studies on their penetration in plants]. 195413151058
[nature of migration of eurygaster integriceps pub. from mountain hibernation places in the krasnodarsk region]. 195114822831
[destructive turtle eurygaster integriceps put. in forrested agricultural regions]. 200615443889
comparative data on the development of reproductive organs in eurygaster integriceps put. in the plains of the south of the ussr and in uzbekistan. 194521026740
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