| molecular characterization of a closterovirus from carrot and its identification as a german isolate of carrot yellow leaf virus. | a high-molecular-weight dsrna (approximately 15 kbp) was isolated from chlorotic leaves of a carrot plant and used for determining the entire nucleotide sequence of a closterovirus. the complete genome of this carrot closterovirus (ccv) was 16.4 kb in length and contained ten open reading frames (orfs). the genome organization of ccv resembled that of beet yellow stunt virus, but orf2 and orf3 were in a reversed order. based on hsp70h sequences, ccv is most closely related to carnation necrotic ... | 2009 | 19575278 |
| screening of the closterovirus genome by degenerate primer-mediated polymerase chain reaction. | the genome of beet yellows virus (byv), the type representative of the closterovirus group, encodes a homologue of the cellular heat-shock protein (hsp) 70 family. a pair of degenerate primers targeted to motifs a and e, which are highly conserved in hsp70s, was synthesized. genomes of several definite and possible members of the closterovirus group were screened for the presence of the hsp70 gene with pcr using these degenerate primers. byv, citrus tristeza virus (ctv), beet yellow stunt virus ... | 1994 | 8207405 |