| preliminary studies on developing a nested pcr assay for molecular diagnosis and identification of nematode (heterakis isolonche) and trematode (glaphyrostomum sp.) in okinawa rail (gallirallus okinawae). | to investigate and detect internal parasites in okinawa rail (gallirallus okinawae), a novel nested polymerase chain reaction with high level of sensitivity and specificity was developed. specific pcr primers that target partial dna sequences of mitochondrial cytochrome c oxidase subunit 1 (cox1) gene were designed to differentiate between nematode (heterakis isolonche) and trematode (glaphyrostomum sp.) found in the intestine of okinawa rail. nested pcr assay was established based on primers de ... | 2009 | 19394146 |
| t-cell lymphoma in a wild okinawa rail (gallirallus okinawae). | the okinawa rail (gallirallus okinawae) is an endangered species that inhabits the northern part of okinawa main island in southern japan. a wild okinawa rail was rescued from a road in kunigami village in okinawa in october 2009. the bird subsequently died and underwent necropsy. tumors were found in the liver, spleen and part of the small intestine. microscopically, lymphoid neoplasm was confirmed in these tissues. the tumor cells were mainly positive for cd3 and cd8α by immunohistochemistry. ... | 2010 | 21060244 |
| reproductive cycle observation of the okinawa rail (gallirallus okinawae) in the wild. | the captive breeding program of the okinawa rail started in 2008. for successful captive breeding, information related to reproduction, such as age at sexual maturity, testicular cycles and ovulatory cycles, is essential to predict when reproduction is possible and when certain reproductive behaviors are most likely to occur. we made gross and histological observations of the reproductive organs of okinawa rails to gain understanding of sexual maturity, the testicular cycle and the ovulatory cyc ... | 2011 | 21606633 |
| genetic diversity and phylogeny of the endangered okinawa rail, gallirallus okinawae. | genetic diversity of the wild population of the endangered okinawa rail, gallirallus okinawae, was revealed by analyzing haplotypes in the mitochondrial control region for 177 individuals. we found 6 haplotypes with nucleotide differences at 6 sites. the four major haplotypes, type 1 to type 4, were present in 121 (68.4%), 21 (11.9%), 8 (4.5%) and 25 individuals (14.1%), respectively. type 5 and type 6 were each found in one individual. the gene diversity (h) and nucleotide diversity (pi) of oki ... | 2010 | 20410665 |
| extended proliferation of chicken- and okinawa rail-derived fibroblasts by expression of cell cycle regulators. | although immortalized cultured cells are useful for various functional assays or transcriptome analysis, highly efficient and reproducible immortalization methods have not been developed in avian-derived cells. we introduced the simian virus 40 t antigen (sv40t) and human papillomavirus (hpv)-e6e7 to chick and okinawa rail (endangered species)derived fibroblast. as a result, neither the sv40t nor e6e7 genes could induce avian cell immortality. accordingly, we attempted to use a recently develope ... | 2019 | 30417340 |
| prevalence of antimicrobial-resistant escherichia coli in endangered okinawa rail (gallirallus okinawae) inhabiting areas around a livestock farm. | antimicrobial resistance (amr) is an important issue for public, animal and environmental health. it has been suggested that livestock farms could be a source origin of amr, and some wild animals that inhabit this area may play an important role in the spread of amr in the natural environment. the prevalence of amr in escherichia coli was examined from okinawa rails (gallirallus okinawae), an endemic bird in okinawa main island, japan. forty-eight faecal samples of wild okinawa rails were collec ... | 2019 | 31452343 |