| in vitro cultivation of microphallus turgidus (trematoda: microphallidae) from metacercaria to ovigerous adult with continuation of the life cycle in the laboratory. | in vitro cultivation of trematodes would aid studies on the basic biology of the parasites and the development of chemotherapies and vaccines. our goal was to measure the in vitro survival and maturation of metacercariae of microphallus turgidus under different culture conditions. metacercariae of m. turgidus from grass shrimp (palaemonetes pugio) were excysted and cultured in humidified air at 37 degrees c in rpmi-1640 medium supplemented with 20% calf, chicken, or horse serum. deposition of eg ... | 2009 | 20049996 |
| optimization of culture conditions for in vitro fertilization and reproduction of microphallus turgidus (trematoda: microphallidae). | most attempts to culture adult digeneans in vitro are unsuccessful. even progenetic digeneans typically fail to produce infective eggs in axenic culture. however, metacercariae of microphallus turgidus grown in vitro mature into adults and release eggs infective to the hydrobiid snail spurwinkia salsa. the objectives of the present study were to verify the reproducibility of the m. turgidus culture protocol, to define optimal culture conditions for m. turgidus further, and to investigate why the ... | 2010 | 21348599 |
| prevalence, geographic distribution, and fitness effects of microphallus turgidus (trematoda: microphallidae) in grass shrimp (palaemonetes spp.) from coastal georgia. | grass shrimp, palaemonetes pugio holthuis and p. vilgaris (say), were collected at 11 localities along the coast of georgia and surveyed for digenetic trematode metacercariae. the effect of trematode infection on grass shrimp fitness was also examined. microphallus turgidas (leigh) was the only trematode observed. the prevalence of metacercarial cysts of this parasite in p. pugio (75%) was higher than in p. vulgaris (24%), as were the mean intensity, abundance, and population density (no. cysts/ ... | 2002 | 12053986 |
| in vitro excystation of metacercariae of microphallus turgidus under different physicochemical conditions. | this study examined the excystation of metacercarial cysts of microphallus turgidus under varying conditions of temperature, nacl concentration, and ph. metacercarial cysts were dissected from abdominal muscle tissue of grass shrimp palaemonetes pugio, and immediately incubated for 24 h under one of the following abiotic conditions: temperature (22, 26, 30, 34, 38, and 42 degrees c), nacl concentrations (0.5, 0.6, 0.7, 0.8, and 0.9%), ph (2, 3, 4, 5, 6, 7, and 8), and percent excystation was rec ... | 2003 | 12733062 |
| effects of the trematode microphallus turgidus on locomotion and prey capture in the grass shrimp palaemonetes pugio. | the grass shrimp, palaemonetes pugio, is common in estuaries and marshes along the east coast of the usa and is frequently infected with metacercariae of the trematode, microphallus turgidus. to test whether or not m. turgidus has an effect on intermediate host behaviour, the length of time spent swimming and walking over 1 min and 3 min intervals and prey (artemia) capture rates of uninfected grass shrimp and those infected with 1-10, 11-20 or 21-30 metacercarial cysts were compared. uninfected ... | 2003 | 14627449 |
| effects of microphallus turgidus (trematoda: microphallidae) on the predation, behavior, and swimming stamina of the grass shrimp palaemonetes pugio. | the effect of the trematode microphallus turgidus on its second intermediate host, the grass shrimp, palaemonetes pugio, was tested. to do so, we measured the susceptibility of infected and uninfected shrimp to predation by the mummichog, fundulus heteroclitus. shrimp behavior was compared in the presence and absence of a fish predator, and the swimming stamina and backthrust escape responses of infected and uninfected shrimp were measured. infected shrimp were more likely to be eaten by a preda ... | 2004 | 15270083 |
| influence of a trematode parasite (microphallus turgidus) on grass shrimp ( palaemonetes pugio ) response to refuge and predator presence. | the grass shrimp ( palaemonetes pugio ) is a staple prey species in the diet of many ecologically and economically important species such as blue crab and striped bass and is commonly infected with a trematode parasite, microphallus turgidus. to complete its life cycle, m. turgidus must be consumed as a metacercaria by a bird or mammal definitive host. previous research has found that infected grass shrimp behave more conspicuously than uninfected shrimp around predators, which include mummichog ... | 2016 | 27560362 |
| behavioral analysis of microphallus turgidus cercariae in relation to microhabitat of two host grass shrimp species (palaemonetes spp.). | the behavior of microphallus turgidus cercariae was examined and compared to microhabitat selection of the second intermediate hosts of the parasite, palaemonetes spp. grass shrimp. cercariae were tested for photokinetic and geotactic responses, and a behavioral ethogram was established for cercariae in control and grass shrimp-conditioned brackish water. photokinesis trials were performed using a half-covered petri dish, and geotaxis trials used a graduated cylinder. both photokinesis and geota ... | 2017 | 28117302 |