| merozoite proteins from babesia sp. bq1 (lintan) as potential antigens for serodiagnosis by elisa. | babesia sp. bq1 (lintan) is a babesia isolated from sheep infested with haemaphysalis qinghaiensis in china, and is closely related to b. motasi based on the 18s rrna gene sequence. in the present study, an elisa was developed with merozoite antigens of babesia sp. bq1 (lintan) (bqma) purified from in vitro culture. when the positive threshold was chosen as 30% of the antibodies rate, evaluated with 198 negative sera, the specificity was 95.5%. except for babesia sp. tianzhu, there was no cross- ... | 2010 | 20109252 |
| novel detection of coxiella spp., theileria luwenshuni, and t. ovis endosymbionts in deer keds (lipoptena fortisetosa). | we describe for the first time the detection of coxiella-like bacteria (clb), theileria luwenshuni, and t. ovis endosymbionts in blood-sucking deer keds. eight deer keds attached to a korean water deer were identified as lipoptena fortisetosa (diptera: hippoboscidae) by morphological and genetic analyses. among the endosymbionts assessed, clb, theileria luwenshuni, and t. ovis were identified in l. fortisetosa by pcr and nucleotide sequencing. based on phylogeny, clb 16s rrna sequences were clas ... | 2016 | 27244561 |
| assessment of exposure to piroplasms in sheep grazing in communal mountain pastures by using a multiplex dna bead-based suspension array. | piroplasms are tick-borne hemoprotozoans with a major impact on extensive management systems. detection of sub-clinical low-level carriers, which can act as source of infection for vector ticks, is key to protect livestock trade and facilitate preventive control programs. the purpose of this study was to develop a method for the detection of ovine piroplasms and to use it in a field study aimed at investigating piroplasms infection in semi-extensive production systems in the basque country (nort ... | 2013 | 24499621 |
| first report of theileria and anaplasma in the mongolian gazelle, procapra gutturosa. | theileria and anaplasma are especially important emerging tick-borne pathogens of animals and humans. molecular surveys and identification of the infectious agents in mongolian gazelle, procapra gutturosa are not only crucial for the species' preservation, but also provide valuable information on parasite and bacterial epidemiology. | 2014 | 25528582 |
| a rapid, simple and sensitive loop-mediated isothermal amplification method to detect anaplasma bovis in sheep and goats samples. | a loop-mediated isothermal amplification (lamp) technique has been widely used in detecting the nucleic acid of various pathogenic bacteria. in this study, a set of four lamp primers was designed to specifically test anaplasma bovis. the lamp assay was performed at 62°c for 60min in a water bath. the specificity was confirmed by amplifying a. bovis isolate, while no cross reaction was observed with other five pathogens (anaplasma bovis, anaplasma phagocytophilum, theileria luwenshuni, babesia mo ... | 2017 | 28351721 |
| development of a real-time pcr assay for detection and quantification of anaplasma ovis infection. | anaplasma ovis is a tick-borne intra-erythrocytic rickettsial pathogen of small ruminants. real-time pcr possesses merits of rapidity, accuracy, reliability, automation and ease of standardization, but has not been used for detection of a. ovis, to the best of our knowledge. in this study, a real-time pcr assay was developed for detection and quantification of a. ovis. species-specific primers and taqman probe were designed based on the glta gene. no cross-reactions were observed with anaplasma ... | 2013 | 24589111 |
| molecular epidemiological survey of bacterial and parasitic pathogens in hard ticks from eastern china. | ticks are able to transmit various pathogens-viruses, bacteria, and parasites-to their host during feeding. several molecular epidemiological surveys have been performed to evaluate the risk of tick-borne pathogens in china, but little is known about pathogens circulating in ticks from eastern china. therefore, this study aimed to investigate the presence of bacteria and parasites in ticks collected from xuzhou, a 11258km(2) region in eastern china. in the present study, ticks were collected fro ... | 2017 | 27986546 |
| detection of theileria luwenshuni in sheep from great britain. | theileria spp. are tick-borne protozoan parasites of the phylum apicomplexa, order piroplasmida that infect a wide range of wild and domestic animals. in great britain, theileria spp. have been reported from livestock associated with transmission by the tick haemaphysalis punctata. however, these reports have not been associated with disease. this study has investigated the cause of a disease outbreak accompanied by mortality in a flock of sheep grazing reclaimed marshland in north kent. | 2016 | 27075661 |
| seroprevalence of bovine theileriosis in northern china. | bovine theileriosis is a common disease transmitted by ticks, and can cause loss of beef and dairy cattle worldwide. here, an indirect enzyme-linked immunosorbent assay (ielisa) based on theileria luwenshuni surface protein (tlsp) was developed and used to carry out a seroepidemiological survey of bovine theileriosis in northern china. | 2016 | 27863521 |
| a study on ovine tick-borne hemoprotozoan parasites (theileria and babesia) in the east black sea region of turkey. | in this study, the frequency of theileria and babesia species was assessed via reverse line blotting and blood smear-based diagnostic methods in small ruminants. a total of 201 apparently healthy animals from 26 randomly selected herds located in 4 locations (artvin, giresun, gumushane, and tokat) of east black sea region of turkey were investigated for the blood protozoans. in a polymerase chain reaction (pcr), the hypervariable v4 region of the 18s ribosomal rna gene was amplified with a set o ... | 2012 | 22231266 |
| prevalence of theileria infections in goats and sheep in southeastern china. | theileriosis is an important tick-borne hemoprotozoan disease, which can cause severe economic loss in animal husbandry. in this paper, one hundred peripheral blood samples of goats and sheep from southeastern china were examined for the theileria infection. a region of theileria 18s rrna gene was amplified by nested pcr in 26 samples. all the nested pcr amplicons were cloned and sequenced. alignment analysis has shown that these sequences are highly homologus to each other with identities from ... | 2011 | 22178410 |
| [morphological comparison of two species of theileria in sheep]. | two splenectomized sheep were infected respectively with theileria luwenshuni and t. uilenbergi, 2 species identified by pcr. when piroplasms were found in blood smears from ears of the sheep, morphological observation on the theileria spp. was carried out by optical microscopy. by giemsa staining, the cytoplasm exhibited in slight blue and nucleus in purple. the two theileria species displayed various shapes, but pyriform-shaped, round-shaped and needle-shaped parasites appeared in every stage ... | 2009 | 19856516 |
| molecular phylogenetic studies on theileria spp. isolates (china) based on small subunit ribosomal rna gene sequences. | six theileria spp. from cattle, buffalo and black goat were identified in the hubei province of china. in order to study the taxonomic status of these parasites, phylogenetic analysis of 18s rrna genes were carried out. the 18s rrna genes from each isolate were amplified by the polymerase chain reaction and the approximate 1.75 kb products were cloned and sequenced. phylogenetic analysis of these gene sequences revealed that the five parasites from buffalo and cattle belonged to the theileria se ... | 2010 | 19557541 |
| detection and differentiation of ovine theileria and babesia by reverse line blotting in china. | a reverse line blot (rlb) assay was developed for detection and specific identification of the different ovine theileria and babesia parasites. in a polymerase chain reaction (pcr), the hypervariable region 4 (v4 region) of the 18s ribosomal dna gene was amplified with a set of general primers specific for members of the genera theileria and babesia. meanwhile, specific oligonucleotide probes were designed and bound on membrane. after one single-pcr amplification, the amplified fragment was hybr ... | 2009 | 19205742 |
| development of loop-mediated isothermal amplification (lamp) assay for rapid diagnosis of ovine theileriosis in china. | loop-mediated isothermal amplification (lamp) is a novel nucleic acid detection method in which the target deoxyribonucleic acid (dna) can be efficiently amplified with high specificity and sensitivity under isothermal conditions using a set of either four or six specific primers. in this study, we have identified a conserved sequence for theileria luwenshuni (utrlu8) and for t. uilenbergi (utru6) suitable for designing a set of six primers for the simultaneous detection by lamp of these pathoge ... | 2008 | 18751728 |
| detection and differentiation of theileria luwenshuni and t. uilenbergi infection in small ruminants by pcr. | theileria luwenshuni and t. uilenbergi are newly identified theileria species highly pathogenic for sheep and goats in china. as they are morphologically indistinguishable and poorly characterized, there is no easily applicable method available to differentiate between these species. in the present study, pcr assays are described for the differentiation and detection of t. luwenshuni and t. uilenbergi using species-specific primers, which were designed based on the hypervariable region of the sm ... | 2008 | 18666967 |
| multiplex pcr for diagnosis of theileria uilenbergi, theileria luwenshuni, and theileria ovis in small ruminants. | infections with theileria sp. may cause significant economic losses to the sheep industry. species identification based on microscopic examination is difficult, and more suitable methods are required for the rapid detection and identification of theileria sp, in clinical specimens. in this study, a multiplex polymerase chain reaction (mpcr) assay was developed to simultaneously identify three individual theileria species in small ruminants. three pairs of specific, sensitive primers were designe ... | 2014 | 24241125 |
| validation of a recombinant protein indirect elisa for the detection of specific antibodies against theileria uilenbergi and theileria luwenshuni in small ruminants. | an enzyme-linked immunosorbent assay (elisa) based on a recombinant theileria uilenbergi immunodominant protein (rtuip) was validated for detection of antibodies in 188 positive and 198 negative reference serum samples, respectively. the cut-off value was determined at 32.7% with 95% and 90% accuracy levels by two-graphic receiver-operating characteristic (tg-roc). the equal diagnostic sensitivity (se) and specificity (sp) were calculated to be 98.4%. further validation of the repeatability with ... | 2014 | 24912957 |
| report of theileria luwenshuni and theileria sp. rsr from cervids in gansu, china. | theileria parasites are important tick-borne pathogens of animals and cause huge economic losses worldwide. here, we undertook to assess the prevalence of theileria spp. in cervids in gansu, china, based on pcr analysis and sequencing of the 18s rrna genes. molecular survey showed that theileria luwenshuni and theileria sp. rsr were frequently found in gansu cervids, and the prevalence of t. luwenshuni was 66.7% (n = 9, 6/9) in roe deer in zhengning county, 58.3% (n = 12, 7/12) in sika deer in n ... | 2015 | 25820647 |
| molecular detection and characterization of theileria infection in cattle and yaks from tibet plateau region, china. | theileriosis continues to threaten the livestock industry worldwide, but comprehensive epidemiological surveys for this disease have not been conducted in the tibet plateau region, china. in this study, we screened 154 cattle blood samples from the tibet plateau region (lhasa, lhoka, and tianzhu), china, for detection of theileria pathogens by polymerase chain reaction (pcr) with species-specific primers. the results revealed that the prevalence was 6.9 % (2/29) for theileria orientalis and 27.6 ... | 2016 | 27000088 |
| comparative proteomic and bioinformatic analysis of theileria luwenshuni and theileria uilenbergi. | theileria is an obligatory intraerythrocytic protozoan parasite that causes economic losses to the cattle, sheep and goats industry. however, very little information is available on the genomes, transcriptomes, and proteomes of the ovine parasites, theileria luwenshuni and theileria uilenbergi. differences in protein expression between these species were investigated to better understand their biology. parasites were digested with trypsin, and the resulting peptides labeled with isobaric tags fo ... | 2016 | 27018062 |
| an indirect elisa for detection of theileria spp. antibodies using a recombinant protein (rtlsp) from theileria luwenshuni. | theileria is a tick-borne, intracellular protozoan parasite of worldwide economic and veterinary importance in small ruminants. here, an enzyme-linked immunosorbent assay (elisa) was developed based on theileria luwenshuni recombinant surface protein (rtlsp) and was used in the standardization and validation of an elisa for the detection of circulating antibodies against ovine and caprine theileriosis. a total of 233 sera samples were used for the calculation of the cut-off value which served as ... | 2016 | 27048941 |
| identification and sequence characterization of novel theileria genotypes from the waterbuck (kobus defassa) in a theileria parva-endemic area in kenya. | waterbuck (kobus defassa), an ungulate species endemic to the eastern african savannah, is suspected of being a wildlife reservoir for tick-transmitted parasites infective to livestock. waterbuck is infested by large numbers of rhipicephalus appendiculatus, the tick vector for theileria parva, and previous data suggests that the species may be a source of t. parva transmission to cattle. in the present study, a total of 86 cattle and 26 waterbuck blood samples were obtained from marula, a site i ... | 2014 | 24690249 |
| genetic variability, phylogenetic evaluation and first global report of theileria luwenshuni, t. buffeli, and t. ovis in sheepdogs in iran. | theileriosis, caused by tick-borne hematozoan organisms, is a protozoan disease of domestic and wild mammals. the most phylo-molecular explorations of genus theileria have been globally focused on the livestock and here are few investigations about canine theileriosis. twenty three and 52 blood samples were collected from sheepdogs and their owners, respectively, at different geographical foci of mazandaran province (east, central, and west regions), northern iran. to primary identify, the taken ... | 2016 | 26997339 |
| identification of 12 piroplasms infecting ten tick species in china using reverse line blot hybridization. | piroplasmosis, a disease of domestic and wild animals is caused by tick-borne protozoa in the genera of theileria and babesia. there was limited information available about the prevalence of piroplasmosis in ticks in china. to assess potential threat of piroplasmosis in china, we investigated the infections of ovine and bovine babesia and theileria species in ticks collected from cattle, yaks, sheep, horses and camels in several regions of china where tick-borne diseases have been reported. a to ... | 2017 | 28355109 |
| evaluating an indirect rmpsp enzyme-linked immunosorbent assay for the detection of bovine theileria infection in china. | bovine theileriosis, a tick-borne protozoan disease caused by theileria annulata, theileria orientalis and theileria sinensis, is widespread in china and is a serious economic problem for the chinese livestock industry. in this study, recombinant major piroplasma surface proteins (mpsp) of t. annulata, t. orientalis and t. sinensis based on mpsp genes were expressed in escherichia coli bl21(de3). the immunogenicity and specificity of the three purified recombinant mpsp proteins were evaluated wi ... | 2017 | 27942962 |
| a loop-mediated isothermal amplification (lamp) assay targeting 16s rrna gene for rapid detection of anaplasma phagocytophilum infection in sheep and goats. | anaplasma phagocytophilum is a zoonotic pathogen and the causative agent of human granulocytic anaplasmosis (hga) in humans and tick-borne fever in various kinds of animals. in the present study, a loop-mediated isothermal amplification (lamp) assay for rapid detection of a. phagocytophilum was developed using primers specific to 16s rrna gene of this organism. the lamp assay was performed at 65 c for 60 min and terminated at 80 c for 10 min. the optimal reaction conditions, under which no cross ... | 2017 | 28118097 |