| lytic activity of the recombinant staphylococcal bacteriophage phih5 endolysin active against staphylococcus aureus in milk. | the endolysin gene (lysh5) from the genome of the staphylococcus aureus bacteriophage phih5 was cloned in escherichia coli and characterized. the lysh5 gene encoded a protein (lysh5) whose calculated molecular mass and pi were 53.7 kda and 8.7, respectively. comparative analysis revealed that lysh5 significantly resembled other murein hydrolases encoded by staphylococcal phages. the modular organization of lysh5 comprised three putative domains, namely, chap (cysteine, histidine-dependent amidoh ... | 2008 | 18809219 |