| physical and chemical characterization of an avian reovirus. | the avian viral agent s1133 has previously been classified serologically as a member of the avian reovirus group. this viral agent grows in chicken embryo fibroblast cells, bands at a density of 1.37 g/ml in cscl equilibrium density gradients, has a particle diameter of 75 nm, and has a morphology similar to that of human reovirus type 3. its nucleic acid is comprised of double-stranded rna and adenosine-rich oligonucleotides. the dsrna is distributed among 10 segments with molecular weights of ... | 1976 | 987252 |
| reovirus type 2 in domestic cats: isolation and experimental transmission. | five reovirus isolates were recovered in ma104 cell cultures from the faeces of three cats with nictitating membrane protrusion and diarrhoea, one cat with diarrhoea only and from one healthy cat. four of these isolates were characterised as reovirus type 2 and one as reovirus type 3 by haemagglutination-inhibition and serum neutralization tests. reovirus type 2 has not been reported previously in cats. mild clinical signs of diarrhoea were noted in kittens infected experimentally with one of th ... | 1992 | 1316666 |
| investigation of genetic interaction between simian rotavirus sa11 and reovirus. | rotavirus and reovirus are physically and genetically very similar. their genetic interaction would have important implications for understanding both the epidemiology of rotavirus and the biological basis of rotavirus pathogenicity. a permissive line of rhesus macaque kidney cells, ma104, was infected with the simian rotavirus sa11 and various temperature sensitive mutants of reovirus (type 3 groups a, b, c, d, g, type 1 m79, and type 2 122). the mixed cell infections were carried out at permis ... | 1982 | 6289051 |
| isolation and characteristics of an equine reovirus type 3 and an antibody prevalence survey to reoviruses in horses located in new york state. | reoviruses have been isolated from a number of species including human, bovine, feline, canine and equine. in most species they seem to produce mild to inapparent disease. we have isolated a reovirus type 3 from a foal with diarrhea. the virus designated the ralph strain has been propagated in both the ma-104 and a-72 cell lines. the strain produced cytoplasmic inclusion bodies in these cell cultures. tissue-cultured virus fixed complement in the presence of reovirus antibodies, but failed to do ... | 1984 | 6326376 |
| an investigation into the health status and diseases of wild dogs (lycaon pictus) in the kruger national park. | many factors have been cited as possible reasons for the decline in the number of wild dogs (lycaon pictus), but few studies have provided supportive data. between 1990 and 1993, the dynamics of 10 wild dog packs in the southern district of the kruger national park in south africa were monitored. casual observations of the causes of disease and mortality in the entire population were also recorded. during the same period, 46 wild dogs were immobilised, weighed, and subjected to physical examinat ... | 1995 | 7629782 |
| diseases and management of backyard chicken flocks in chitungwiza, zimbabwe. | to gather information on backyard chicken flocks in chitungwiza, an urban center in zimbabwe, 85 flock owners were interviewed. the mean flock size was 53 birds (range 1-650), and most birds were kept for meat, for either domestic consumption or local sale. mean age at slaughter was 12.4 weeks (range 8-24). none of the owners vaccinated their birds, and reported mortality rates were high (mean 25%), most commonly being associated with diseases causing eye and respiratory problems. most owners co ... | 1994 | 7832719 |
| murine viruses in an island population of introduced house mice and endemic short-tailed mice in western australia. | house mice (mus domesticus) were recently introduced to thevenard island, off the northwest coast of western australia. this island is also habitat for an endangered native rodent, the short-tailed mouse (leggadina lakedownensis). concerns have been raised that house mice may pose a threat to l. lakedownensis both through competition and as a source of infection. to assess the threat to l. lakedownensis posed by viral pathogens from m. domesticus, a serological survey was conducted from 1994 to ... | 1999 | 10231757 |
| serological evidence of murine pathogens in wild grey squirrels (sciurus carolinensis) in north wales. | wild grey squirrels in north wales were examined for their serological response to a range of laboratory mouse pathogens. nineteen squirrels were tested against 14 organisms and a high seroprevalence was found to several, including murine cytomegalovirus, mouse adenovirus, reovirus 3, rotavirus and sendai virus. four of the squirrels were seropositive for lymphocytic choriomeningitis virus, a zoonotic pathogen. none of the infections has previously been reported in squirrels. | 2002 | 12019534 |
| viral susceptibility of newly established cell lines from the hawaiian monk seal monachus schauinslandi. | ten of 11 cell lines, recently established from the snout (ms-sn), periorbital soft tissue (ms-ey), liver (ms-lv), kidney (ms-kd), lung (ms-lg), spleen (ms-sp), heart (ms-ht), thyroid (ms-ty), brain (ms-br) and urinary bladder (ms-ub) of a juvenile hawaiian monk seal monachus schauinslandi, were evaluated in vitro for their susceptibility to 5 mammalian viruses: herpes simplex virus type 1 (hsv-1), vesicular stomatitis virus (vsv), reovirus type 3 (reo-3), poliovirus type 1 (polio-1) and vaccini ... | 2003 | 14960030 |
| a chimeric adenovirus vector encoding reovirus attachment protein sigma1 targets cells expressing junctional adhesion molecule 1. | the utility of adenovirus (ad) vectors for gene transduction can be limited by receptor specificity. we developed a gene-delivery vehicle in which the potent ad5 vector was genetically reengineered to display the mucosal-targeting sigma1 protein of reovirus type 3 dearing (t3d). a sigma1 construct containing all but a small virion-anchoring domain was fused to the n-terminal 44 aa of ad5 fiber. this chimeric attachment protein fibtail-t3dsigma1 forms trimers and assembles onto ad virions. fibtai ... | 2004 | 15079060 |
| efficacy and safety evaluation of human reovirus type 3 in immunocompetent animals: racine and nonhuman primates. | human reovirus type 3 has been proposed to kill cancer cells with an activated ras signaling pathway. the purpose of this study was to investigate the efficacy of reovirus in immunocompetent glioma animal models and safety/toxicity in immunocompetent animals, including nonhuman primates. | 2004 | 15623640 |
| hereditary hydrocephalus in laboratory-reared golden hamsters (mesocricetus auratus). | a colony of golden hamsters had an ongoing problem with hydrocephalus. in an attempt to clear the colony of the problem, new breeders from another supplier had been purchased. at termination of a behavioral study, the brain was collected from 35 animals (four of which had died with hydrocephalus during the study) and was examined macroscopically and by light microscopy. although no animals manifested obvious behavioral changes, 31 of 35 (88.6%, 13/15 males and 18/20 females in control and manipu ... | 2006 | 16846994 |
| microbiological monitoring of guinea pigs reared conventionally at two breeding facilities in korea. | in this study, microbiological monitoring of guinea pigs reared conventionally in two facilities was performed twice in 2004, with a three-month-interval between surveys. this study was based on the recommendations of the felasa working group, with some modifications. in serological tests in the first survey, some animals from facility a showed positive results for encephalitozoon cuniculi, sendai virus, pneumonia virus of mice (pvm), and reovirus-3 (reo-3); facility b showed a positive result o ... | 2006 | 17090958 |
| cation exchange chromatography performed in overloaded mode is effective in removing viruses during the manufacturing of monoclonal antibodies. | viral safety is a critical concern with regard to monoclonal antibody (mab) products produced in mammalian cells such as chinese hamster ovary cells. manufacturers are required to ensure the safety of such products by validating the clearance of viruses in downstream purification steps. cation exchange (cex) chromatography is widely used in bind/elute mode as a polishing step in mab purification. however, bind/elute modes require a large volume of expensive resin. to reduce the production cost, ... | 2019 | 31148380 |
| prevalence of naturally occurring viral infections, mycoplasma pulmonis and clostridium piliforme in laboratory rodents in western europe screened from 2000 to 2003. | in this report prevalence rates of rodent viruses in laboratory animals are presented based on routine serological screening of mouse and rat colonies from european institutes. the prevalences found during the period 2000-2003 are compared with those reported for 1981-1984 and 1990-1993. it is shown that some infections were eliminated from laboratory animal colonies (e.g. k-virus and polyomavirus) by taking preventative measures whereas other infections such as mouse hepatitis virus and parvovi ... | 2006 | 16600073 |
| a serological indication of the existence of a guineapig poliovirus. | attempts were made to clarify whether laboratory guineapigs may harbour a poliovirus which, in 1911, was described as the cause of a disease called guineapig lameness. by the use of elisa for antibodies against the poliovirus, theiler's murine encephalomyelitis virus (tmev), it was shown that two pet shop guineapigs suffering from lameness had extremely high titres against poliovirus, while healthy guineapigs from the same pet shop were negative. clearly positive results were also found in 35 ou ... | 1997 | 9230501 |
| infection of reovirus type 3 in mongolian gerbils (meriones unguiculatus)--lesions in pancreas and brain. | gerbils were inoculated with reovirus type 3 (abney strain), and the pancreas, brain and other organs histopathologically evaluated. male mongolian gerbils (meriones unguiculatus) aged 5-10 days (newborn, 9 animals), 4 weeks (juvenile, 5 animals), and 12 weeks (adult, 5 animals) were used. gerbils inoculated intraperitoneally with the virus were sacrificed 3, 5, 7, or 10 days later. tissues were observed by light and electron microscopy. the pancreas of newborns showed inflammatory edema with in ... | 1993 | 8393111 |
| extrahepatic biliary atresia in a rhesus monkey (macaca mulatta). | extrahepatic biliary atresia was observed in a 6-week-old female rhesus monkey. jaundice and conjugated hyperbilirubinemia were detected at the age of 6 days and persisted throughout life. at 6 weeks of age, the diagnosis of extrahepatic biliary atresia was established at exploratory laparotomy, and bile duct remnants were biopsied. histological examination of these specimens showed inflammatory and fibrosing lesions similar to those observed in humans with extrahepatic biliary atresia. because ... | 1983 | 6862370 |
| differential interaction of reovirus type 3 with sialylated receptor components on animal cells. | in this report we study the interaction of reovirus type 3 dearing (rv3) with vertebrate erythrocytes whose membrane glycoconjugates differ in the degree and position of o-acetylation of their sialic acid (neuac) residues. binding to erythrocytes required the presence of neuac on cellular glycoconjugates, since pretreatment with sialidase (neuraminidase) abolished hemagglutination by rv3. furthermore, we found that rv3 binds efficiently to and hemagglutinates all erythrocyte preparations possess ... | 1987 | 3672931 |
| serologic study on the prevalence of murine viruses in a population of wild meadow voles (microtus pennsylvanicus). | the demography and serology of a population of wild meadow voles (microtus pennsylvanicus) were monitored from 1982 to 1984 near pinawa, manitoba, canada. serologic tests were performed on 486 samples to detect the presence of viral antibodies to 11 common murine viruses. meadow voles showed evidence of infection with theiler's encephalomyelitis, reovirus-type-3, ectromelia, lymphocytic choriomeningitis, adenovirus, and mouse hepatitis viruses. at times of good survival and breeding performance ... | 1986 | 3525873 |
| seromonitoring in small laboratory animal colonies. a five year survey: 1984-1988. | from 1984 to 1988 one thousand serologic investigations of laboratory animal colonies originating from 10 different european countries were performed. the most prevalent infections in mouse stocks were caused by mouse hepatitis virus (mhv), minute virus of mice (mvm), theiler's encephalomyelitis virus (temv), reovirus type 3 (reo3), sendaivirus, and pneumonia virus of mice (pvm). in mice no infections with lymphocytic choriomeningitis virus (lcm), polyomavirus, mouse adenovirus, and k-virus were ... | 1990 | 2321435 |
| [serological study of the incidence of murine viruses in a population of small wild rodents (microtus pennsylvanicus ord, 1815)]. | the results of a serological survey of a free-living population of meadow voles (microtus pennsylvanicus) in pinawa, manitoba (canada) showed that these animals possessed antibodies to six of the eleven viruses tested for, namely: reovirus type 3, murine encephalomyelitis agent, ectromelia virus, murine adenovirus, murine hepatitis virus and lymphocytic choriomeningitis virus. the significant increase in the number of individuals possessing specific antibodies suggests that these viruses, or rel ... | 1992 | 1339064 |
| seroprevalence of rodent pathogens in wild rats from the island of st. kitts, west indies. | a pilot seroprevalence study was conducted to document exposure to selected pathogens in wild rats inhabiting the caribbean island of st. kitts. serum samples collected from 22 captured wild rats (rattus norvegicus and rattus rattus) were tested for the presence of antibodies to various rodent pathogens using a rat mfi2 serology panel. the samples were positive for cilia-associated respiratory bacillus (13/22; 59.1%), clostridium piliforme (4/22; 18.2%), mycoplasma pulmonis (4/22; 18.2%), pneumo ... | 2019 | 31083284 |
| isolation of reovirus t3d mutants capable of infecting human tumor cells independent of junction adhesion molecule-a. | mammalian reovirus is a double-stranded rna virus with a distinctive preference to replicate in and lyse transformed cells. on that account, reovirus type 3 dearing (t3d) is clinically evaluated as oncolytic agent. the therapeutic efficacy of this approach depends in part on the accessibility of the reovirus receptor junction adhesion molecule-a (jam-a) on the target cells. here, we describe the isolation and characterization of reovirus t3d mutants that can infect human tumor cells independent ... | 2012 | 23110175 |
| a novel pathogenic mammalian orthoreovirus from diarrheic pigs and swine blood meal in the united states. | since may 2013, outbreaks of porcine epidemic diarrhea have devastated the u.s. swine industry, causing immense economic losses. two different swine enteric coronaviruses (porcine epidemic diarrhea virus and delta coronavirus) have been isolated from the affected swine population. the disease has been reported from at least 32 states of the united states and other countries, including mexico, peru, dominican republic, canada, columbia, ecuador, and ukraine, with repeated outbreaks in previously ... | 2015 | 25991685 |