| characterization of tomato leaf curl purple vein virus, a new monopartite new world begomovirus infecting tomato in northeast brazil. | a new begomovirus species was identified from tomato plants with upward leaf curling and purple vein symptoms, which was first identified in the piaui state of northeast (ne) brazil in 2014. tomato leaf samples were collected in 2014 and 2016, and pcr with degenerate primers revealed begomovirus infection. rolling circle amplification and restriction enzyme digestion indicated a single genomic dna of ~ 2.6 kb. cloning and sequencing revealed a genome organization similar to dna-a components of n ... | 2017 | 29224131 |