| analysis of transcripts from predicted open reading frames of musca domestica salivary gland hypertrophy virus. | the musca domestica salivary gland hypertrophy virus (mdsghv) is a large dsdna virus that infects and sterilizes adult houseflies. the transcriptome of this newly described virus was analysed by rapid amplification of cdna 3'-ends (3'-race) and rt-pcr. direct sequencing of 3'-race products revealed 78 poly(a) transcripts containing 95 of the 108 putative orfs. an additional six orfs not amplified by 3'-race were detected by rt-pcr. only seven of the 108 putative orfs were not amplified by either ... | 2009 | 19264592 |
| musca domestica salivary gland hypertrophy virus, a globally distributed insect virus that infects and sterilizes female houseflies. | the housefly, musca domestica, is a cosmopolitan pest of livestock and poultry and is of economic, veterinary, and public health importance. populations of m. domestica are naturally infected with m. domestica salivary gland hypertrophy virus (mdsghv), a nonoccluded double-stranded dna virus that inhibits egg production in infected females and is characterized by salivary gland hypertrophy (sgh) symptoms. mdsghv has been detected in housefly samples from north america, europe, asia, the caribbea ... | 2010 | 20023109 |
| tissue tropism of the musca domestica salivary gland hypertrophy virus. | the tissue tropism of musca domestica salivary gland hypertrophy virus (mdsghv) infecting adult house flies was examined by transmission electron microscopy (tem) and quantitative real-time pcr. tem demonstrated that characteristic mdsghv-induced nuclear and cellular hypertrophy was restricted to the salivary glands. both nucleocapsids and enveloped virions were present in salivary gland cells. in contrast, thin sections of midguts, ovaries, abdominal fat body, crops, air sacs and brains showed ... | 2011 | 20600389 |
| Disease Dynamics and Persistence of Musca domestica Salivary Gland Hypertrophy Virus Infections in Laboratory House Fly (Musca domestica) Populations. | Past surveys of feral house fly populations have shown that Musca domestica salivary gland hypertrophy virus (MdSGHV) has a worldwide distribution, with an average prevalence varying between 0.5% and 10%. How this adult-specific virus persists in nature is unknown. In the present study, experiments were conducted to examine short-term transmission efficiency and long-term persistence of symptomatic MdSGHV infections in confined house fly populations. Average rates of disease transmission from vi ... | 2012 | 22057863 |
| salivary gland hypertrophy virus of house flies in denmark: prevalence, host range, and comparison with a florida isolate. | house flies (musca domestica) infected with musca domestica salivary gland hypertrophy virus (mdsghv) were found in fly populations collected from 12 out of 18 danish livestock farms that were surveyed in 2007 and 2008. infection rates ranged from 0.5% to 5% and averaged 1.2%. none of the stable flies (stomoxys calcitrans), rat-tail maggot flies (eristalis tenax) or yellow dung flies (scathophaga stercoraria) collected from mdsghv-positive farms displayed characteristic salivary gland hypertroph ... | 2011 | 22129394 |
| impact of house fly salivary gland hypertrophy virus (mdsghv) on a heterologous host, stomoxys calcitrans. | the effect of musca domestica salivary gland hypertrophy virus (mdsghv) on selected fitness parameters of stable flies, stomoxys calcitrans (l.), was examined in the laboratory. virus-injected stable flies of both genders suffered substantially higher mortality than control flies. by day 9, female mortality was 59.3 +/- 10.1% in the virus group compared with 23.7 +/- 3.7% in the controls; mortality in virus-injected males was 78.1 +/- 3.1% compared with 33.3 +/- 9.3% for controls. fecundity of c ... | 2011 | 22238871 |