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n-alkanes as a substratum for riboflavin production. i. investigations of the dynamics of the flavinogenesis in chosen yeasts of the genus candida.for the first time the flavinogenic abilities of candida flareri on a simple, fully defined medium with hydrocarbons were demonstrated. c flareri and c. guilliermondi on a synthetic medium with biotin and n-alkanes (n-decane and hexadecane) overproduce riboflavin with a considerable but varying efficiency. among the two hydrocarbons examined n-hexadecane proved to be a more favourable source of carbon for the biosynthesis of this vitamin than n-decane. the quantities of riboflavin accumulated by ...1978643742
the metabolism of aromatic acids by micro-organisms. a reassessment of the role of o-benzoquinone as a product of protocatechuate metabolism by fungi.1. the addition of aniline to cultures of several yeasts, fusarium oxysporum and neurospora crassa growing with protocatechuate as sole carbon source resulted in the precipitation of dianilino-o-benzoquinone (anil). this product was also formed, however, if the medium was uninoculated. 2. the physical presence of yeast cells (living or dead) increased the anil yields in debaryomyces subglobosus cultures. 3. no anil was formed if p-hydroxybenzoate was the growth substrate. 4. o-benzoquinone was a ...19685691755
studies on the biosynthesis of riboflavin. 5. general factors controlling flavinogenesis in the yeast candida flareri. 195913651123
studies on the biosynthesis of riboflavin. 7. the incorporation of adenine and guanine into riboflavin and into nucleic acid purines in eremothecium ashbyii and candida flareri. 196213863203
the production of riboflavin and d-arabitol by debaryomyces subglobosus. 196314072830
construction and fed-batch cultivation of candida famata with enhanced riboflavin production.riboflavin (vitamin b2) is an essential nutrition component serving as a precursor of coenzymes fmn and fad that are involved mostly in reactions of oxidative metabolism. riboflavin is produced in commercial scale and is used in feed and food industries, and in medicine. the yeast candida famata (candida flareri) belongs to the group of so called "flavinogenic yeasts" which overproduce riboflavin under iron limitation. three genes sef1, rib1 and rib7 coding for a putative transcription factor, g ...201424361297
influence of storage on the fungal concentration determination of impinger and filter samples.the effects of storage on concentrations of airborne fungal samples were evaluated in a laboratory test chamber. spores of penicillium citrinum and cells of candida famata var. flareri were aerosolized by a pitt-3 generator and a collison nebulizer, respectively. the evaluated sampling methods were agi-30 impingers, nuclepore filters, and gelatin filters. the effect of storage time was determined as the ratio, c(t)/c(0), where c(t) and c(0) were the total counts or colony concentrations of the s ...200312570402
[ascospore formation in yeasts related to torulopsis candida].ascospore formation was found in the type strains torulopsis famata (harrison) lodder et kreger van rij, t. minor (pollaci et nannizi) lodder and candida flareri (ciferri et redaelli) langeron et guerra. the names of these species should be considered as a synonym of debaryomyces hansenii (zopf) lodder et kreger van rij.19836686647
candida famata (candida flareri).candida famata (candida flareri) belongs to the group of so-called 'flavinogenic yeasts', capable of riboflavin oversynthesis under condition of iron starvation. some strains of c. famata belong to the most flavinogenic organisms known and were used for industrial production of riboflavin for a long time in the usa. c. famata is characterized by high salt tolerance, growing at nacl concentrations of up to 2.5  m. development of basic tools for the metabolic engineering of c. famata, such as a tr ...201223108915
polyphasic re-examination of debaryomyces hansenii strains and reinstatement of d. hansenii, d. fabryi and d. subglobosus.the type species of the genus debaryomyces, debaryomyces hansenii, is a highly heterogeneous species. it has been isolated from a large diversity of natural sources including fruit, air, water, soil, but most frequently from processed food products. the species delineation of this yeast species urgently needs clarification. the heterogeneity in taxonomic characteristics has resulted in the description of varieties linked to d. hansenii. the aim of this study was to re-examine and classify d. han ...200820396575
delimitation of the species of the debaryomyces hansenii complex by intron sequence analysis.the delineation of species among strains assigned to debaryomyces hansenii was examined using a gene genealogies-based approach in order to compare spliceosomal intron sequences found in four housekeeping genes (act1, tub2, rpl31 and rpl33). this revealed four distinct groups of strains containing, respectively, d. hansenii var. hansenii cbs 767(t), d. hansenii var. fabryi cbs 789(t), candida famata var. flareri cbs 1796(t) (the anamorph of d. hansenii var. fabryi cbs 789(t)) and debaryomyces ty ...200919406826
differentiation of debaryomyces hansenii and candida famata by rrna gene intergenic spacer fingerprinting and reassessment of phylogenetic relationships among d. hansenii, c. famata, d. fabryi, c. flareri (=d. subglobosus) and d. prosopidis: description of d. vietnamensis sp. nov. closely related to d. nepalensis.the intergenic spacer rdna amplification and alui fingerprinting (igsaf) method detected four distinct groups among 170 debaryomyces hansenii strains: d. hansenii var. hansenii; candida famata var. famata; d. hansenii var. fabryi and c. famata var. flareri. igs sequence comparison of representative strains showed that d. hansenii var. hansenii and c. famata var. famata belonged to one species, whereas d. hansenii var. fabryi and c. famata var. flareri belonged to two different ones. this confirm ...200919385997
development of species-specific primers for rapid identification of debaryomyces hansenii.in this work, we developed a specific pcr assay for debaryomyces hansenii strains that uses a putative homologous pad1 region (729 bp) present in this yeast species as a target. the amplification of this sequence with the d. hansenii specific primer pair (dhpadf/dhpadr) was found to be a rapid, specific and an affordable method enabling identification of d. hansenii from other yeast strains. primers were tested in almost 100 strains, 49 strains from type culture collection belonging to the genus ...201525462930
metabolic engineering and classic selection of the yeast candida famata (candida flareri) for construction of strains with enhanced riboflavin production.currently, the mutant of the flavinogenic yeast candida famata dep8 isolated by classic mutagenesis and selection is used for industrial riboflavin production. here we report on construction of a riboflavin overproducing strain of c. famata using a combination of random mutagenesis based on the selection of mutants resistant to different antimetabolites as well as rational approaches of metabolic engineering. the conventional mutagenesis involved consecutive selection for resistance to riboflavi ...201021040798
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