| Growth of Esteya vermicola in media amended with nitrogen sources yields conidia with increased predacity and resistance to environmental stress. | Esteya vermicola , an endoparasitic fungus of pinewood nematode, exhibits great potential as a biological agent against nematodes. In this study to enhance the sporulation, predacity, and environmental resistance of E. vermicola, various nitrogen sources, such as glycine, L-leucine, and ammonium nitrate, were tested. The supplement of glycine and L-leucine had a significant influence on the growth rate of the colony, enhancing colony dry mass by 5-fold more than did ammonium nitrate or the contr ... | 2011 | 21942397 |
| the effects of fluctuating culture temperature on stress tolerance and antioxidase expression in esteya vermicola. | the endoparasitic nematophagous fungus, esteya vermicola, has shown great potential as a biological control agent against the pine wood nematode, bursaphelenchus xylophilus. fluctuating culture temperatures can affect fungal yields and fungal tolerance to desiccation, uv radiation, h2o2, and heat stress, as well as antioxidase expression. to explore these effects, e. vermicola cultured under five temperature ranges, 26°c, 15-26°c, 26-35°c, 20-30°c, and 15-35°c, were compared. the cultures grown ... | 2015 | 25626367 |
| optimization of storage condition for maintaining long-term viability of nematophagous fungus esteya vermicola as biocontrol agent against pinewood nematode. | the fungus, esteya vermicola has been proposed as biocontrol agent against pine wilting disease caused by bursaphelenchus xylophilus. in this study, we reported the effects of temperature and different additives on the viability and biocontrol efficacy of e. vermicola formulated by alginate-clay. the viability of the e. vermicola formulation was determined for six consecutive months at temperature ranged from -70 to 25 °c. the fresh conidia without any treatment were used as control. under the o ... | 2014 | 25070159 |
| optimization of promoting conidial production of a pinewood nematode biocontrol fungus, esteya vermicola using response surface methodology. | hydrogen peroxide was applied for promoting sporulation of esteya vermicola and response surface methodology was used to optimize the effect of processing parameters on sporulation. three variables were concentration (x 1), treatment time (x 2), and carbon-to-nitrogen ratio (x 3). the results indicated that x 1 and x 2 and the quadratic term of x 1 had significant effect on the sporulation, followed by the significant interaction effects between x 1 and x 2. the optimal conditions of promoting s ... | 2014 | 25002361 |
| a staining method for assessing the viability of esteya vermicola conidia. | the viability of conidia of esteya vermicola, a potentially important biocontrol agent against the pinewood nematode bursaphelenchus xylophilus, is usually determined by cultivation for 18-48 h in culture medium. as an alternative to this labor-intensive method, we have developed a rapid, simple, and low-cost staining method for assessing e vermicola conidia survival rates. a mixture of neutral red and methylene blue was found to be the most optimal among several stains that also included safran ... | 2014 | 24585076 |
| host deception: predaceous fungus, esteya vermicola, entices pine wood nematode by mimicking the scent of pine tree for nutrient. | a nematophagous fungus, esteya vermicola, is recorded as the first endoparasitic fungus of pine wood nematode (pwn), bursaphelenchus xylophilus, in last century. e. vermicola exhibited high infectivity toward pwn in the laboratory conditions and conidia spraying of this fungus on japanese red pine, pinus densiflora, seedlings in the field protected the pine trees from pine wilt disease to some extent, indicating that it is a potential bio-control agent against pwn. previous research had demonstr ... | 2013 | 23990972 |
| comparison between conidia and blastospores of esteya vermicola, an endoparasitic fungus of the pinewood nematode, bursaphelenchus xylophilus. | esteya vermicola, an endoparasitic fungus of bursaphelenchus xylophilus, the pinewood nematode (pwn), exhibits great potential as a biological control agent against this nematode. e. vermicola produces blastospores in liquid media and aerial conidia on solid media. the agent was mass-produced using two kinds of culture media: s (50 % wheat bran and 50 % pine wood powder), l (0.5 g wheat bran and 0.5 g pinewood powder in 200 ml of potato dextrose broth), and two controls: sc (potato dextrose agar ... | 2013 | 23918632 |
| attraction of pinewood nematode to endoparasitic nematophagous fungus esteya vermicola. | the investigations on attraction of nematodes to nematophagous fungi have mostly dealt with the nematode-trapping species. esteya vermicola is the endoparasitic fungus of pinewood nematode (pwn) with high infection activity. in the present study, the attraction of pwns to e. vermicola was investigated. it was confirmed that the living mycelia and exudative substances of e. vermicola were attractive to pwn. compared with the nematode-trapping fungus a. brochopaga as well as nematode-feeding fungu ... | 2010 | 20012046 |
| effect of nutrition and environmental factors on the endoparasitic fungus esteya vermicola, a biocontrol agent against pine wilt disease. | the nematophagous fungus esteya vermicola has tremendous potential for biological control. this species exhibits strong infectious activity against pinewood nematodes, whereas the study on the effect of nutrition and environmental factors is still of paucity. carbon (c), nitrogen (n), ph value, temperature, and water activity have great impact on the fungal growth, sporulation, and germination. in nutrition study, the greatest number of conidia (2.36 × 10(9) per colony) was obtained at the c:n r ... | 2013 | 23595506 |
| microbial transformation of ginsenoside rg3 to ginsenoside rh2 by esteya vermicola cnu 120806. | in the present investigation, we successfully employed a cell-free extract of esteya vermicola cnu 120806 to convert ginsenoside rg3 to rh2. three important factors including ph, temperature and substrate concentration were optimized for the preparation of rh(2). the optimal condition was obtained as follows: 50°c, ph 5.0 and substrate concentration of 3 mg ml(-1). the yield of conversion was up to 90.7%. in order to identify the specificity of the β-glucosidase activity of esteya vermicola cnu ... | 2012 | 22805964 |
| highly selective microbial transformation of major ginsenoside rb1 to gypenoside lxxv by esteya vermicola cnu120806. | this study examined the biotransformation pathway of ginsenoside rb(1) by the fungus esteya vermicola cnu 120806. | 2012 | 22805203 |
| high infectivity of an endoparasitic fungus strain, esteya vermicola, against nematodes. | esteya vermicola, as the first recorded endoparasitic fungus of pinewood nematodes, exhibits great potential as a biological agent against nematodes. however, only two strains of this species have been described so far. in this study, we identified a novel endoparasitic fungal strain, cnu 120806, isolated from infected nematodes in forest soil samples during a survey of nematophagous fungi in korea. this strain showed similar morphological characteristics and infection mode with the two previous ... | 2008 | 18758727 |