Publications

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prevalence of horse tapeworm in north london and hertfordshire. 19873590566
enzyme-linked immunosorbent assay (elisa) for the detection of antibodies to anoplocephala perfoliata in horse sera.a scolex antigen of the horse tapeworm anoplocephala perfoliata containing at least 14 different proteins was employed in an enzyme-linked immunosorbent assay (elisa) for detection of antibodies to a. perfoliata in equine sera. the assay was applied to sera from 426 slaughtered horses with different numbers of worms and with varying degrees of intestinal lesions. as measured by the elisa, there was a very strong effect on the antibody levels both from the number of tapeworms present and from the ...19957483241
anoplocephala perfoliata in horses in sweden: prevalence, infection levels and intestinal lesions.distal ileum, caecum and proximal colon of 470 horses were examined for helminths during 1 year at an abattoir in central sweden. the infection levels of the horse tapeworm anoplocephala perfoliata, their stage of development, site of attachment and gross pathological lesions caused by the worm were recorded. faecal samples from 395 of the horses were examined specifically for tapeworm segments and eggs in order to correlate these findings with the numbers in the alimentary canal. in total 65% o ...19957502948
observations on the echinococcus granulosus horse strain in spain.the spanish sheep and horse strains of echinococcus granulosus possess several differential characteristics in their metacestode stage. cysts from sheep vary widely in size and fertility, but they usually have a thin cyst wall and, when fertile, a whitish hydatid sand formed by brood capsules and protoscoleces. two types of infections have been observed in horses: one resembling that of sheep, caused by small, non-fertile cysts with a thin wall, and a second type caused by medium to large, alway ...19989653991
changing behaviour in the common horse tapeworm. 200314667092
a pcr system for detection of species and genotypes of the echinococcus granulosus-complex, with reference to the epidemiological situation in eastern africa.we describe the development of a specific and sensitive pcr/semi-nested pcr system for the rapid diagnosis of echinococcus granulosus genotype g1, e. granulosus genotype g6/7, and echinococcus ortleppi (g5). diagnosis of g1 and the group g5/6/7 is performed by a simple pcr, while discrimination between e. ortleppi (g5) and g6/7 involves a subsequent semi-nested pcr step. the target sequence for amplification is part of the mitochondrial 12s rrna gene. specificity of the pcrs was 100% when evalua ...200415064129
the present situation of echinococcosis in europe.the taxonomy of echinococcus is presently undergoing major changes, the paraphyletic echinococcus granulosus being split into several distinct species. in this review, an attempt is made to assess the present epidemiological situation in europe separately for each species (echinococcus multilocularis, echinococcus granulosus sensu stricto, echinococcus equinus, echinococcus ortleppi, and echinococcus sp.). for e. multilocularis, an increasing density of infected host animals is apparent in centr ...200616352465
epidemiology and control of echinococcosis in sub-saharan africa.cystic echinococcosis (ce) is highly endemic among the nomadic pastoral tribes of east africa, but is rare amongst the agriculturally based communities. echinococcus granulosus infections are common in dogs from all countries in sub-saharan africa where they have been examined. transmission of e. granulosus to humans is affected by such factors as prevalence of the parasite in domestic dogs, behaviors of humans towards dogs, and heterogenicity of the parasite and susceptibility of humans to infe ...200616406684
cystic echinococcosis in equids in italy.between march 2003 and february 2007, the livers and the lungs of 2,231 horses from various italian regions were examined for cystic echinococcosis presence at the time of slaughter. hydatid cysts were found in six horses, namely four from sardinia, one from sicily, and one from tuscany. the location, number, morphology, and fertility of the cysts found were determined. dna was extracted from the germinal layers and protoscoleces of the fertile cysts and polymerase chain reactions (pcr) were per ...200818180956
cystic echinococcosis due to echinococcus equinus in a horse from southern germany.in europe, cystic echinococcosis is rare in horses and is mostly diagnosed at slaughter or postmortem examination. equine cystic echinococcosis can be caused by various echinococcus taxa, but only echinococcus equinus (the "horse strain") is known to produce fertile cysts. in europe, e. equinus appears to be endemic in great britain, ireland, spain, and italy and has sporadically been reported in belgium and switzerland. the present report describes the first case of a molecularly confirmed e. e ...201020453228
ecology and life cycle patterns of echinococcus species.the genus echinococcus is composed of eight generally recognized species and one genotypic cluster (echinococcus canadensis cluster) that may in future be resolved into one to three species. for each species, we review existing information on transmission routes and life cycles in different geographical contexts and - where available - include basic biological information of parasites and hosts (e.g., susceptibility of host species). while some echinococcus spp. are transmitted in life cycles th ...201728131364
first study about the development of adult echinococcus canadensis g6 genotype of goat origin in experimentally infected dogs.echinococcus granulosus sensu lato (e. granulosus sl) must be considered as a species complex, comprising echinococcus granulosus sensu stricto (e. granulosus ss, genotypes g1-g3), echinococcus equinus (g4), echinococcus ortleppi (g5) and echinococcus canadensis (g6-g10) although the species status of e. canadensis is still controversial. these genotypes closely match the intermediate hosts associated strains described in earlier times among which e. canadensis g6 corresponds to the camel strain ...201627692331
a novel pcr-rflp assay for molecular characterization of echinococcus granulosus sensu lato and closely related species in developing countries.cystic echinococcosis, due to echinococcus granulosus sensu lato (s. l.), currently affects three million people, especially in low-income countries and results in high livestock production loss. dna-based methods demonstrated genetic variability of e. granulosus s. l., and five species were recognized to belong to the complex, including e. granulosus sensu stricto (s.s) (genotypes g1-g3), echinococcus equinus (genotype g4), echinococcus ortleppi (genotype g5), echinococcus canadensis (genotypes ...201627225001
taxonomy and molecular epidemiology of echinococcus granulosus sensu lato.echinococcus granulosus, formerly regarded as a single species with a high genotypic and phenotypic diversity, is now recognised as an assemblage of cryptic species, which differ considerably in morphology, development, host specificity (including infectivity/pathogenicity for humans) and other aspects. this diversity is reflected in the mitochondrial and nuclear genomes and has led to the construction of phylogenetic trees and hypotheses on the origin and geographic dispersal of various taxa. b ...201526264250
the first report of human-derived g10 genotype of echinococcus canadensis in china and possible sources and routes of transmission.cystic echinococcosis (ce) is one of the most important parasitic zoonoses. 10 distinct genotypes, designated g1-g10 genotypes of echinococcus granulosus sensu lato (s.l.), have been split into 4 species: echinococcus granulosus sensu stricto (s.s.) (g1-g3), echinococcus equinus (g4), echinococcus ortleppi (g5) and echinococcus canadensis (g6-g10); echinococcus felidis has also been suggested as a sister taxon of e. granulosus s.s. recently. four genotypes belonging to two species (g1 and g3 gen ...201525967082
echinococcus granulosus sensu lato genotypes infecting humans--review of current knowledge.genetic variability in the species group echinococcus granulosus sensu lato is well recognised as affecting intermediate host susceptibility and other biological features of the parasites. molecular methods have allowed discrimination of different genotypes (g1-10 and the 'lion strain'), some of which are now considered separate species. an accumulation of genotypic analyses undertaken on parasite isolates from human cases of cystic echinococcosis provides the basis upon which an assessment is m ...201424269720
reprint of "echinococcus granulosus sensu stricto (s.s.) from the critically endangered antelope addax nasomaculatus in tunisia".echinococcus granulosus sensu lato (s.l.) is a zoonotic disease highly endemic in tunisia. canids including stray and semi-stray dogs, jackals and foxes are known as definitive hosts and a wide range of ungulates have been shown to harbour the metacestode hydatid stage and may serve as intermediate hosts. fertile hydatid cysts of echinococcus equinus and e. granulosus sensu stricto (s.s.) were recently molecularly identified for the first time from tunisian donkeys. e. granulosus (s.s.) was also ...201727887695
fatal echinococcosis in three lemurs in the united kingdom--a case series.tapeworms of the genus echinococcus reside in the small intestine of a number of carnivorous species, predominantly canids. in enzootic areas, hydatidosis caused by taeniid metacestodes can present a significant problem in accidental intermediate hosts, including humans. whereas the united kingdom is currently considered free of echinococcus multilocularis, echinococcus granulosus sensu stricto (s.s.) and echinococcus equinus are endemic in the uk and have been reported in a variety of captive m ...201626872922
first report of echinococcus equinus in a donkey in turkey.a 2-year-old female donkey (equus asinus) was euthanized in the pathology department of firat university, elazig, turkey. necropsy disclosed the presence of 7 hydatid cysts distributed throughout the lung parenchyma. one of those cysts represented the parasite material of the present study and was molecularly identified through sequencing of a fragment of cytochrome c oxidase subunit 1 (co1) and nicotinamide adenine dinucleotide dehydrogenase subunit 1 (nadh1) gene, as echinococcus equinus. the ...201526797441
echinococcus granulosus sensu stricto (s.s.) from the critically endangered antelope addax nasomaculatus in tunisia.echinococcus granulosus sensu lato (s.l.) is a zoonotic disease highly endemic in tunisia. canids including stray and semi-stray dogs, jackals and foxes are known as definitive hosts and a wide range of ungulates have been shown to harbour the metacestode hydatid stage and may serve as intermediate hosts. fertile hydatid cysts of echinococcus equinus and e. granulosus sensu stricto (s.s.) were recently molecularly identified for the first time from tunisian donkeys. e. granulosus (s.s.) was also ...201526314229
a sylvatic lifecycle of echinococcus equinus in the etosha national park, namibia.various species of echinococcus have been described in the past from wild mammals of sub-saharan africa. however, it is only recently, that a few isolates have become available for molecular identification; therefore, the involvement of wildlife in the lifecycles of the various cryptic species within echinococcus granulosus sensu lato is still only partially known. a preliminary survey was undertaken in etosha national park, namibia, from august to october 2012. faecal samples were obtained from ...201525830103
echinococcus equinus and echinococcus granulosus sensu stricto from the united kingdom: genetic diversity and haplotypic variation.cystic echinococcosis is endemic in europe including the united kingdom. however, information on the molecular epidemiology of echinococcus spp. from the united kingdom is limited. echinococcus isolates from intermediate and definitive animal hosts as well as from human cystic echinococcosis cases were analysed to determine species and genotypes within these hosts. echinococcus equinus was identified from horse hydatid isolates, cysts retrieved from captive uk mammals and copro-dna of foxhounds ...201525479251
first detection and molecular characterization of echinococcus equinus in a mule in turkey.cystic echinococcosis is a zoonotic disease with a cosmopolital distribution. it is caused by the larval stages (metacestodes) of the parasite echinococcus granulosus which infects different animal species. in this report, we present a case of e. granulosus infection in a mule and molecular characterization of the cyst. for this purpose parasite material was collected from the liver of a necropsied mule. dna was isolated and pcr amplification of mitochondrial 12s rrna as well as partial sequenci ...201425236292
genetic variability and haplotypes of echinococcus isolates from tunisia.the species/genotypes of echinococcus infecting a range of intermediate, canid and human hosts were examined as well as the intraspecific variation and population structure of echinococcus granulosus sensu lato (s.l.) within these hosts.201425213876
modelling the transmission dynamics of cystic echinococcosis in donkeys of different ages from tunisia.during the period from march 2006 to july 2009 a total of 2040 slaughtered donkeys were examined for cystic echinococcosis (ce). ce prevalence in donkeys was 8.48% and the infection pressure (0.0088 infections per year) and infection rate (0.0448 cysts per year) appeared to be lower than those previously reported for cattle, sheep, dromedaries and goats in tunisia. however, the number of cysts per infection was relatively high (5.07 cysts per infection). among the 901 collected hydatid cysts the ...201424986435
molecular characterization of echinococcus granulosus in egyptian donkeys.the present study was carried out during the period from august 2009 to july 2010. the purpose of this study was to identify the genotype of donkeys' echinococcosis in one of the egyptian governorates. on post-mortem inspection taking place at the zoo of beni-suef, egypt, a total number of 145 donkeys were examined for the presence of hydatid cysts. ten of these donkeys were found to be infected by hydatid cysts; and location, number and fertility of cysts found were determined. the liver was th ...201323246076
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