| genetic characterization and phylogenetic position of echinococcus felidis (cestoda: taeniidae) from the african lion. | echinococcus felidis had been described in 1937 from african lions, but was later included in echinococcus granulosus as a subspecies or a strain. in the absence of any genetic characterization, most previous records of this taxon from a variety of large african mammals remained unconfirmed due to the lack of diagnostic criteria and the possible confusion with the sympatric e. granulosus sensu stricto, echinococcus ortleppi and echinococcus canadensis. in this study, we obtained taeniid eggs fro ... | 2008 | 18062973 |
| an evaluation of antigen b family of echinococcus granulosus, its conformational propensity and elucidation of the agretope. | the present communication evaluates the antigen b (agb) family of bubaline isolates of echinococcus granulosus with respect to their conformational propensity and also discusses the stretches of agretope. agb, which is abundantly present in hydatid cyst fluid, is encoded by a gene family, agb1-agb5. hydatidosis is of zoonotic and economic importance in india. buffaloes serve as the intermediate host. however, to date the agb family has not been fully analysed. during the present study two differ ... | 2009 | 19102812 |
| a survey of echinococcus species in wild carnivores and livestock in east africa. | we examined 71 faecal samples of carnivores from queen elizabeth national park (qenp), uganda, for eggs of echinococcus species. thirty-nine faecal samples contained taeniid eggs. for species diagnosis, dna was isolated from a total of 1984 individual taeniid eggs. to differentiate eggs of echinococcus felidis from other taeniid taxa (including the closely related echinococcus granulosus sensu stricto), a restriction fragment length polymorphism (rflp)-pcr of the mitochondrial nad1 gene was deve ... | 2009 | 19275902 |
| a molecular survey of cystic echinococcosis in sudan. | a survey of cystic echinococcosis in livestock was conducted from may 2001 to july 2003 in central, western and southern sudan. hydatid cysts were present in 59% (466/779) of camels, 6% (299/4893) of cattle, 11% (1180/10,422) of sheep and 2% (106/5565) of goats, with little variation among different geographical areas. 532 of these cysts were examined by pcr and could be overwhelmingly (98.7%) allocated to echinococcus canadensis g6/7 (all of 215 cysts from camels, 112 of 114 cysts from cattle, ... | 2010 | 20138433 |
| echinococcus canadensis g7 (pig strain): an underestimated cause of cystic echinococcosis in austria. | anamnesis data of 104 patients with cystic echinococcosis were correlated retrospectively with the detected species/strain of echinococcus. ninety-two percent (n = 23) of autochthonous austrian and 33% (n = 9) of patients with former yugoslavian (yu) origin were infected with e. canadensis g7, the pig strain. all patients originating from turkey harbored e. granulosus g1, the sheep strain. all e. canadensis g7-infected patients showed small liver cysts (ø 5.9 cm), only one of them an additional ... | 2010 | 20439969 |
| genetic classification of echinococcus granulosus cysts from humans, cattle and camels in libya using mutation scanning-based analysis of mitochondrial loci. | we genetically classified echinococcus granulosus from humans, cattle and camels in libya utilizing dna regions (designated pcox1 and pnad1) within the cytochrome c oxidase subunit 1 (cox1) and nadh dehydrogenase 1 (nad1) mitochondrial genes, respectively. polymerase chain reaction (pcr)-based single-strand conformation polymorphism (sscp) analysis of pcox1 and pnad1 amplicons derived from genomic dna samples from individual cysts (n = 176) revealed four distinct electrophoretic profiles for eac ... | 2010 | 20659552 |
| molecular characterization of echinococcus isolates indicates goats as reservoir for echinococcus canadensis g6 genotype in neuquén, patagonia argentina. | human cystic echinococcosis is a highly endemic zoonotic disease in the province of neuquén, patagonia argentina, although a hydatid control programme has been carried out since 1970. human infection due to echinococcus canadensis (g6 genotype) is frequent in neuquén. however, the reservoir for this species remains undetermined in a region where camels are absent. we investigated the fertility, viability and molecular epidemiology of hydatid cysts obtained from local goats, pigs and sheep in ord ... | 2010 | 20667482 |
| a first insight into the genotypes of echinococcus granulosus from humans in mongolia. | polymerase chain reaction (pcr)-based single-strand conformation polymorphism (sscp) and targeted sequencing were employed to genetically classify echinococcus granulosus cysts from humans from 12 provinces in mongolia using two dna loci, designated pcox-1 and pnad-1, within the mitochondrial cytochrome c oxidase subunit 1 (cox-1) and nadh dehydrogenase subunit 1 (nad-1) genes, respectively. sscp analysis of pcox-1 and pnad-1 amplicons produced from genomic dna samples from individual e. granulo ... | 2010 | 21075201 |
| a real-time multiplex-nested pcr system for coprological diagnosis of echinococcus multilocularis and host species. | a hybridization probe-based real-time multiplex-nested pcr system was developed for the simultaneous detection of echinococcus multilocularis and host species directly from faecal samples. species identification was determined by melting curve analysis. specificity was assessed by using dna extracted from various cestodes (e. multilocularis, echinococcus granulosus (g1), echinococcus ortleppi, echinococcus canadensis (g6, g7), taenia crassiceps, taenia hydatigena, taenia mustelae, taenia pisifor ... | 2011 | 21327991 |
| A molecular survey on cystic echinococcosis in Sinnar area, Blue Nile state (Sudan). | Cystic echinococcosis (CE) is a zoonosis caused by the cestodes of the Echinococcus species. Its life cycle involves dogs and other canids as definitive hosts for the intestinal tapeworm, as well as domestic and wild ungulates as intermediate hosts for the tissue-invading metacestode (larval) stage. The disease has a special impact on disadvantaged pastoralist communities and is listed now among the three top priority neglected tropical disease (NTD). Therefore, CE is a neglected disease even in ... | 2011 | 22040487 |
| characterisation of antigen b protein species present in the hydatid cyst fluid of echinococcus canadensis g7 genotype. | the larva of cestodes belonging to the echinococcus granulosus sensu lato (s.l.) complex causes cystic echinococcosis (ce). it is a globally distributed zoonosis with significant economic and public health impact. the most immunogenic and specific echinococcus-genus antigen for human ce diagnosis is antigen b (agb), an abundant lipoprotein of the hydatid cyst fluid (hf). the agb protein moiety (apolipoprotein) is encoded by five genes (agb1-agb5), which generate mature 8 kda proteins (agb8/1-agb ... | 2017 | 28045899 |
| neglected zoonotic helminths: hymenolepis nana, echinococcus canadensis and ancylostoma ceylanicum. | the majority of helminth parasites that are considered by who to be the cause of 'neglected diseases' are zoonotic. in terms of their impact on human health, the role of animal reservoirs and polyparasitism are both emerging issues in understanding the epidemiology of a number of these zoonoses. as such, hymenolepis (rodentolepis) nana, echinococcus canadensis and ancylostoma ceylanicum all qualify for consideration. they have been neglected and there is increasing evidence that all three parasi ... | 2015 | 25743998 |
| the echinococcus canadensis (g7) genome: a key knowledge of parasitic platyhelminth human diseases. | the parasite echinococcus canadensis (g7) (phylum platyhelminthes, class cestoda) is one of the causative agents of echinococcosis. echinococcosis is a worldwide chronic zoonosis affecting humans as well as domestic and wild mammals, which has been reported as a prioritized neglected disease by the world health organisation. no genomic data, comparative genomic analyses or efficient therapeutic and diagnostic tools are available for this severe disease. the information presented in this study wi ... | 2017 | 28241794 |
| global distribution of alveolar and cystic echinococcosis. | alveolar echinococcosis (ae) and cystic echinococcosis (ce) are severe helminthic zoonoses. echinococcus multilocularis (causative agent of ae) is widely distributed in the northern hemisphere where it is typically maintained in a wild animal cycle including canids as definitive hosts and rodents as intermediate hosts. the species echinococcus granulosus, echinococcus ortleppi, echinococcus canadensis and echinococcus intermedius are the causative agents of ce with a worldwide distribution and a ... | 2017 | 28131365 |
| ecology and life cycle patterns of echinococcus species. | the genus echinococcus is composed of eight generally recognized species and one genotypic cluster (echinococcus canadensis cluster) that may in future be resolved into one to three species. for each species, we review existing information on transmission routes and life cycles in different geographical contexts and - where available - include basic biological information of parasites and hosts (e.g., susceptibility of host species). while some echinococcus spp. are transmitted in life cycles th ... | 2017 | 28131364 |
| phylogenetic pattern, evolutionary processes and species delimitation in the genus echinococcus. | an accurate and stable alpha taxonomy requires a clear conception of what constitutes a species and agreed criteria for delimiting different species. an evolutionary or general lineage concept defines a species as a single lineage of organisms with a common evolutionary trajectory, distinguishable from other such lineages. delimiting evolutionary species is a two-step process. in the first step, phylogenetic reconstruction identifies putative species as groups of organisms that are monophyletic ... | 2017 | 28131362 |
| echinococcus granulosus sensu stricto, echinococcus canadensis (g7), and echinococcus ortleppi in fertile hydatid cysts isolated from cattle in southern brazil. | echinococcosis is a cosmopolitan zoonotic infection that affects humans and animals. the aim of this study was to identify and characterize the fertile hydatid cysts from bovine viscera in order to verify different species and/or genotypes present in southern brazil. firstly, cysts were collected from a slaughterhouse, which received animals from different regions of rio grande do sul state (rs), considered an important area of occurrence of cystic echinococcosis. in total, 2396 cysts were analy ... | 2016 | 27554941 |
| first study about the development of adult echinococcus canadensis g6 genotype of goat origin in experimentally infected dogs. | echinococcus granulosus sensu lato (e. granulosus sl) must be considered as a species complex, comprising echinococcus granulosus sensu stricto (e. granulosus ss, genotypes g1-g3), echinococcus equinus (g4), echinococcus ortleppi (g5) and echinococcus canadensis (g6-g10) although the species status of e. canadensis is still controversial. these genotypes closely match the intermediate hosts associated strains described in earlier times among which e. canadensis g6 corresponds to the camel strain ... | 2016 | 27692331 |
| identification of <i>echinococcus granulosus</i> strains using polymerase chain reaction-restriction fragment length polymorphism amongst livestock in moroto district, uganda. | a descriptive study was conducted to identify the different strains of echinococcus granulosus occurring in livestock in moroto district, uganda. echinococcus cysts from 104 domestic animals, including cattle, sheep, goats and camels, were taken and examined by microscopy, polymerase chain reaction with restriction fragment length polymorphism and sanger dna sequencing. echinococcus granulosus genotypes or strains were identified through use of bioinformatics tools: bioedit, blast and mega6. the ... | 2016 | 27543147 |
| molecular identification of echinococcus granulosus isolates from ruminants in greece. | cystic echinococcosis is a parasitic disease caused by echinococcus granulosus, a cestode with worldwide distribution. data on the circulating echinococcus granulosus genotypes in greek livestock is scant. the aim of the present study was to conduct a genetic analysis of 82 echinococcus granulosus isolates from ruminants in greece, including areas which until today have not been the subject of studies. the analysis relied on a pcr assay targeting cytochrome c oxidase, subunit 1 gene (co1), follo ... | 2016 | 27514899 |
| a dual pcr-based sequencing approach for the identification and discrimination of echinococcus and taenia taxa. | reliable and rapid molecular tools for the genetic identification and differentiation of echinococcus species and/or genotypes are crucial for studying spatial and temporal transmission dynamics. here, we describe a novel dual pcr targeting regions in the small (rrns) and large (rrnl) subunits of mitochondrial ribosomal rna (rrna) genes, which enables (i) the specific identification of species and genotypes of echinococcus (rrns + l-pcr) and/or (ii) the identification of a range of taeniid cesto ... | 2016 | 27242008 |
| cystic echinococcosis: future perspectives of molecular epidemiology. | human cystic echinococcosis (ce) has been considered to be caused predominantly by echinococcus granulosus sensu stricto (the dog-sheep strain). molecular approaches on ce, however, have revealed that human cases are also commonly caused by another species, echinococcus canadensis. all indices for classification and standardization of ce pathology including available images, epidemiology, diagnostics and treatment are currently based largely on a mixture of infections which include at least e. g ... | 2017 | 27237060 |
| a novel pcr-rflp assay for molecular characterization of echinococcus granulosus sensu lato and closely related species in developing countries. | cystic echinococcosis, due to echinococcus granulosus sensu lato (s. l.), currently affects three million people, especially in low-income countries and results in high livestock production loss. dna-based methods demonstrated genetic variability of e. granulosus s. l., and five species were recognized to belong to the complex, including e. granulosus sensu stricto (s.s) (genotypes g1-g3), echinococcus equinus (genotype g4), echinococcus ortleppi (genotype g5), echinococcus canadensis (genotypes ... | 2016 | 27225001 |
| first insights into the genetic diversity of echinococcus granulosus sensu stricto (s.s.) in serbia. | cystic echinococcosis (ce) caused by echinococcus granulosus sensu stricto (s.s.) is a cosmopolitan zoonotic infection which is endemic in serbia where it is subject to mandatory reporting. however, information on the incidence of the disease in humans and prevalence of hydatid infection in livestock remains limited. we used sequenced data of the cytochrome c oxidase subunit 1 (cox 1) mitochondrial gene to examine the genetic diversity and population structure of e. granulosus (s.s.) from interm ... | 2016 | 27198778 |
| a novel zoonotic genotype related to echinococcus granulosus sensu stricto from southern ethiopia. | complete mitochondrial and two nuclear gene sequences of a novel genotype (gomo) related to echinococcus granulosus sensu stricto are described from a metacestode isolate retrieved from a human patient in southwestern ethiopia. phylogenetically, the genotype is positioned within the e. granulosus sensu stricto/echinococcus felidis cluster, but cannot easily be allocated to either species. based on different mitochondrial dna markers, it is closest to the haplotype cluster that currently defines ... | 2016 | 27181929 |
| molecular characterization of echinococcus granulosus sensu stricto and echinococcus canadensis in humans and livestock from algeria. | in algeria, previous studies investigated genotypes of echinococcus granulosus sensu lato in animals and identified e. granulosus sensu stricto (s.s.) genotypes g1 and g3 whereas echinococcus canadensis genotype g6 was only reported from dromedary cysts. molecular data on human cystic echinococcosis (ce) were limited. we implemented a large genotyping study of hydatid cysts from humans and livestock animals to specify ce's molecular epidemiology and the genetic diversity in algeria. fifty-four h ... | 2016 | 27021186 |
| first report of echinococcus granulosus (genotype g6) in a dog in bamako, mali. | cystic echinococcosis is one of the most widespread and important helminthic zoonoses, caused by the larval stage of echinococcus granulosus sensu lato. however, to date there is little information about the disease in west africa. faecal and fur samples from 193 dogs, the main final hosts, were collected in 2010 and 2011 in bamako, mali. taeniid eggs were found microscopically in 28/118 (24%) and 80/223 (36%) faecal and fur samples, respectively. one faecal and one fur sample from the same dog ... | 2016 | 26827862 |
| first molecular evidence of the simultaneous human infection with two species of echinococcus granulosus sensu lato: echinococcus granulosus sensu stricto and echinococcus canadensis. | cystic echinococcosis is a widespread zoonotic parasitic disease especially in tunisia which is one of the most endemic countries in the mediterranean area. the etiological agent, echinococcus granulosus sensu lato, implies dogs and other canids as definitive hosts and different herbivore species as intermediate hosts. human contamination occurs during the consumption of parasite eggs passed in the environment through canid feces. hydatid cysts coming from a child operated for multiple echinococ ... | 2016 | 26612498 |
| an autochthonous case of cystic echinococcosis in finland, 2015. | we report a case of pulmonary cystic echinococcosis in a child from eastern finland with no history of travelling abroad. the cyst was surgically removed and the organism molecularly identified as echinococcus canadensis genotype g10. this parasite is maintained in eastern finland in a sylvatic life cycle involving wolves and moose; in the present case, the infection was presumably transmitted by hunting dogs. | 2015 | 26538367 |
| cases of echinococcus granulosus sensu stricto isolated from polish patients: imported or indigenous? | the cases of nine polish patients with diagnosed cystic echinococcosis (ce) were examined. a total of nine isolates obtained postoperatively were investigated using pcr and sequencing. the mitochondrial region of nad1 gene was amplified. this pcr and sequencing analysis revealed the presence of echinococcus canadensis g7 in seven patients and e. granulosus g1 in two patients. these data demonstrate that e. canadensis is the predominant causative agent of human cystic echinococcosis in poland. e. ... | 2015 | 26491683 |
| taxonomy and molecular epidemiology of echinococcus granulosus sensu lato. | echinococcus granulosus, formerly regarded as a single species with a high genotypic and phenotypic diversity, is now recognised as an assemblage of cryptic species, which differ considerably in morphology, development, host specificity (including infectivity/pathogenicity for humans) and other aspects. this diversity is reflected in the mitochondrial and nuclear genomes and has led to the construction of phylogenetic trees and hypotheses on the origin and geographic dispersal of various taxa. b ... | 2015 | 26264250 |
| echinococcus canadensis transmission in the north. | the echinococcus granulosus complex (eg) is the causative agent of cystic echinococcosis (ce). northern cervid echinococcus was previously suggested to be the ancestor of the entire eg. during the last century, it was regarded to have three (or four) different, but often overlapping, transmission cycles in the circumpolar north: the original wolf-wild cervid (reindeer or elk)-cycle; the semi-synanthropic cycle involving sled and hunting dogs and wild cervids; and the synanthropic cycle involving ... | 2015 | 26264249 |
| the present situation of echinococcoses in mongolia. | this review presents the historical and current situation of echinococcoses in mongolia. since the collapse of the soviet union in 1991, mongolia's health surveillance infrastructure has been very poor, especially as it pertains to chronic diseases, including neglected zoonotic diseases (nzds). although there is anecdotal evidence of people dying from hepatic disease due to infection with the larval stage of echinococcus spp., there are very few published reports. all confirmed cases of echinoco ... | 2015 | 26234999 |
| the first report of human-derived g10 genotype of echinococcus canadensis in china and possible sources and routes of transmission. | cystic echinococcosis (ce) is one of the most important parasitic zoonoses. 10 distinct genotypes, designated g1-g10 genotypes of echinococcus granulosus sensu lato (s.l.), have been split into 4 species: echinococcus granulosus sensu stricto (s.s.) (g1-g3), echinococcus equinus (g4), echinococcus ortleppi (g5) and echinococcus canadensis (g6-g10); echinococcus felidis has also been suggested as a sister taxon of e. granulosus s.s. recently. four genotypes belonging to two species (g1 and g3 gen ... | 2015 | 25967082 |
| microrna profiling in the zoonotic parasite echinococcus canadensis using a high-throughput approach. | micrornas (mirnas), a class of small non-coding rnas, are key regulators of gene expression at post-transcriptional level and play essential roles in fundamental biological processes such as development and metabolism. the particular developmental and metabolic characteristics of cestode parasites highlight the importance of studying mirna gene regulation in these organisms. here, we perform a comprehensive analysis of mirnas in the parasitic cestode echinococcus canadensis g7, one of the causat ... | 2015 | 25656283 |
| surveillance of echinococcus isolates from qinghai, china. | echinococcosis is highly endemic over large parts of the qinghai-tibet plateau (qtp), china. based on a large number of samples, we present data on the current presence, host distribution, and genetic diversity of echinococcus in the qinghai province, located in the northeastern corner of the qtp and constituting >25% of the area of the plateau. we used 521 samples (including 451 newly collected samples and 70 samples from our previous study) from humans, yaks, sheep, goats, dogs, fox, plateau p ... | 2015 | 25480467 |
| echinococcus canadensis, e. borealis, and e. intermedius. what's in a name? | the phylogenetic relationships of the g6, g7, g8, and g10 genotypes of echinococcus granulosus are well defined, but their taxonomic status is currently unresolved. we apply an evolutionary species concept to infer that the g6 and g7 genotypes represent a single species that is different to both the g8 and g10 genotypes, and that the g8 and g10 genotypes are also on different evolutionary trajectories and, therefore, should be regarded as separate species. the names echinococcus intermedius, ech ... | 2015 | 25440521 |
| short report: genetic variation of echinococcus canadensis (g7) in mexico. | we evaluated the genetic variation of echinococcus g7 strain in larval and adult stages using a fragment of the mitochondrial cox1 gen. viscera of pigs, bovines, and sheep and fecal samples of dogs were inspected for cystic and canine echinococcosis, respectively; only pigs had hydatid cysts. bayesian inferences grouped the sequences in an e. canadensis g7 cluster, suggesting that, in mexico, this strain might be mainly present. additionally, the population genetic and network analysis showed th ... | 2014 | 25266350 |
| echinococcus canadensis (g7) and echinococcus granulosus sensu stricto (g1) in swine of southern brazil. | the cystic echinococcosis (ce) is an important zoonotic disease caused by the parasite echinococcus spp. in brazil, this parasite is present in rio grande do sul (rs) state, border with argentina and uruguay, causing several damages to human and animal health. this study aimed to identify echinococcus spp. in hydatid cysts of swine and evaluate the similarity of the genotypes through the phylogenetic analysis. a total of 3,101,992 swine were slaughtered in the central/northern region of rs/brazi ... | 2014 | 24566128 |
| pigs and wild boar in corsica harbor echinococcus canadensis g6/7 at levels of concern for public health and local economy. | cystic echinococcosis (ce) is a parasitic zoonosis widespread in the mediterranean area. the parasite is commonly maintained in a domestic cycle involving dogs and livestock species. as no new data have been made available for the last 15 years concerning the french mediterranean island of corsica, a cross-sectional survey at the slaughterhouse was conducted in 2009-2010 to describe the current presence of echinococcus granulosus sensu lato in intermediate hosts. only pig infections with the g6/ ... | 2014 | 24556138 |
| surveillance for echinococcus canadensis genotypes in canadian ungulates. | the geographic and host distribution, prevalence and genotypes of echinococcus canadensis in wild ungulates in canada are described to better understand the significance for wildlife and public health. we observed e. canadensis in 10.5% (11/105) of wild elk (wapiti; cervus canadensis) in riding mountain national park, manitoba, examined at necropsy, over two consecutive years (2010-2011). molecular characterization of hydatid cyst material from these elk, as well as three other intermediate wild ... | 2013 | 24533321 |
| rapid detection of echinococcus species by a high-resolution melting (hrm) approach. | high-resolution melting (hrm) provides a low-cost, fast and sensitive scanning method that allows the detection of dna sequence variations in a single step, which makes it appropriate for application in parasite identification and genotyping. the aim of this work was to implement an hrm-pcr assay targeting part of the mitochondrial cox1 gene to achieve an accurate and fast method for echinococcus spp. differentiation. | 2013 | 24517106 |
| balancing selection and heterozygote advantage in major histocompatibility complex loci of the bottlenecked finnish wolf population. | maintaining effective immune response is an essential factor in the survival of small populations. one of the most important immune gene regions is the highly polymorphic major histocompatibility complex (mhc). we investigated how a population bottleneck and recovery have influenced the diversity and selection in three mhc class ii loci, dla-drb1, dla-dqa1 and dla-dqb1, in the finnish wolf population. we studied the larger russian karelian wolf population for comparison and used 17 microsatellit ... | 2014 | 24382313 |
| echinococcus granulosus sensu lato genotypes infecting humans--review of current knowledge. | genetic variability in the species group echinococcus granulosus sensu lato is well recognised as affecting intermediate host susceptibility and other biological features of the parasites. molecular methods have allowed discrimination of different genotypes (g1-10 and the 'lion strain'), some of which are now considered separate species. an accumulation of genotypic analyses undertaken on parasite isolates from human cases of cystic echinococcosis provides the basis upon which an assessment is m ... | 2014 | 24269720 |
| echinococcus multilocularis and echinococcus canadensis in wolves from western canada. | echinococcus species are important parasites of wildlife, domestic animals and people worldwide; however, little is known about the prevalence, intensity and genetic diversity of echinococcus tapeworms in canadian wildlife. echinococcus tapeworms were harvested from the intestines of 42% of 93 wolves (canis lupus) from five sampling regions in the northwest territories, manitoba and saskatchewan, and visually identified to genus level by microscopic examination. genetic characterization was succ ... | 2014 | 24135428 |
| echinococcus species from red foxes, corsac foxes, and wolves in mongolia. | the small intestines of 420 wild canids (111 corsac foxes, 191 red foxes and 118 wolves) from mongolia, were examined for adult worms of the genus echinococcus. the mongolian genotype of echinococcus multilocularis was found in fifteen red foxes and four wolves, whereas two genotypes (g6/7 and g10) of echinococcus canadensis were found in two and three wolves, respectively. no adult echinococcus worms were found in the corsac foxes examined. the genotypes of e. multilocularis and e. canadensis a ... | 2013 | 23953219 |
| first report on circulation of echinococcus ortleppi in the one humped camel (camelus dromedaries), sudan. | echinococcus granulosus (eg) complex, the cause of cystic echinococcosis (ce), infects humans and several other animal species worldwide and hence the disease is of public health importance. ten genetic variants, or genotypes designated as (g1-g10), are distributed worldwide based on genetic diversity. the objective of this study was to provide some sequence data and phylogeny of eg isolates recovered from the sudanese one-humped camel (camelus dromedaries). fifty samples of hydatid cysts were c ... | 2013 | 23800362 |
| mitochondrial phylogeny of the genus echinococcus (cestoda: taeniidae) with emphasis on relationships among echinococcus canadensis genotypes. | the mitochondrial genomes of the genus echinococcus have already been sequenced for most species and genotypes to reconstruct their phylogeny. however, two important taxa, e. felidis and e. canadensis g10 genotype (fennoscandian cervid strain), were lacking in the published phylogeny. in this study, the phylogeny based on mitochondrial genome sequences was completed with these taxa. the present phylogeny highly supports the previous one, with an additional topology showing sister relationships b ... | 2013 | 23731519 |
| molecular diagnosis of cystic echinococcosis in humans from central poland. | the identity of the causative agent of cystic echinococcosis (ce) in humans from central poland receiving treatment between 2000 and 2010 was determined. a total of 47 samples obtained after hepatectomy were examined and protoscoleces were identified in wet preparations in 27 cases. using dna extracted from the samples, two mitochondrial regions (nad1 and cox1 genes) were amplified and the nad1 fragment was sequenced. this pcr analysis confirmed the presence of echinococcus species in 30 cases a ... | 2013 | 23535071 |
| molecular characterization of echinococcus granulosus in south-eastern romania: evidence of g1-g3 and g6-g10 complexes in humans. | clin microbiol infect | 2013 | 22967309 |
| prevalence and diversity of cystic echinococcosis in livestock in maasailand, kenya. | cystic echinococcosis (ce) is a zoonotic disease caused by several members of the echinococcus granulosus species complex. in east africa, several species/strains are known to occur in livestock and humans, but host preferences, relative frequencies and spatial distribution of these taxa are poorly known. here, we contribute livestock data for maasailand of southern kenya. total ce prevalence was 25.8 % in cattle (151/587), 16.5 % in sheep (71/430) and 10.8 % in goats (21/194), which is a signif ... | 2012 | 22915272 |
| molecular identification of human echinococcosis in the altai region of russia. | mitochondrial haplotypes were determined for echinococcus species infecting individuals diagnosed with alveolar echinococcosis (ae) and cystic echinococcosis (ce) at altai state medical university hospital in barnaul, russia during 2008 to 2011. the nucleotide sequence of the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene was determined for 31 of 34 ae and 8 of 12 ce cases. all of the ae cases were confirmed to be caused by asian type echinococcus multilocularis, while ce cases were ca ... | 2012 | 22609955 |
| molecular identification of unilocular hydatid cysts from domestic ungulates in ethiopia: implications for human infections. | to identify the etiologic agents of cystic echinococcosis in ethiopia, unilocular hydatid cysts were collected from 11 sheep, 16 cattle and 16 camels slaughtered in abattoirs of aweday, jijiga, haramaya and addis ababa during june 2010 to february 2011. a pcr-based dna sequencing of the mitochondrial cytochrome oxidase c subunit 1 gene (cox1) was conducted for 40 cysts. the majority of cysts (87.5%) were identified as echinococcus granulosus sensu stricto and the rest as echinococcus canadensis. ... | 2012 | 22329916 |
| seasonal and biogeographical patterns of gastrointestinal parasites in large carnivores: wolves in a coastal archipelago. | parasites are increasingly recognized for their profound influences on individual, population and ecosystem health. we provide the first report of gastrointestinal parasites in gray wolves from the central and north coasts of british columbia, canada. across 60 000 km(2), wolf feces were collected from 34 packs in 2005-2008. at a smaller spatial scale (3300 km(2)), 8 packs were sampled in spring and autumn. parasite eggs, larvae, and cysts were identified using standard flotation techniques and ... | 2012 | 22309976 |
| specific status of echinococcus canadensis (cestoda: taeniidae) inferred from nuclear and mitochondrial gene sequences. | the specific status of echinococcus canadensis has long been controversial, mainly because it consists of the mitochondrial lineages g6, g7, g8 and g10 with different host affinity: g6 (camel strain) and g7 (pig strain) with domestic cycles and g8 (cervid strain) and g10 (fennoscandian cervid strain) with sylvatic or semi-domestic cycles. there is an argument whether the mitochondrial lineages should be recognised as separate species which correspond to the biological or epidemiological aggregat ... | 2017 | 28797792 |
| genetic differentiation of the g6/7 cluster of echinococcus canadensis based on mitochondrial marker genes. | among the genotype/species causing cystic echinococcosis, the taxonomic status of echinococcus canadensis is only partially resolved. within e. canadensis, four genotypes (g6, g7, g8 and g10) have been described based on short mitochondrial sequences, of which g6 and g7 (the 'camel' and the 'pig' strain, respectively) are closely related and variously regarded as microvariants of a single strain g6/7. globally, this g6/7 cluster is the second most important agent of human cystic echinococcosis a ... | 2017 | 28780151 |
| genetic characterization of human hydatid cysts shows coinfection by echinococcus canadensis g7 and echinococcus granulosus sensu stricto g1 in argentina. | human cystic echinococcosis caused by the larval stage of echinococcus granulosus sensu lato (s.l.) is a highly endemic disease in the province of neuquén, patagonia, argentina. human infections with e. granulosus sensu stricto (s.s.) g1 and echinococcus canadensis g6 were reported in neuquén in previous studies, whereas four genotypes were identified in livestock: g1, g3, g6, and g7. the aim of this study was to identify the genotypes of e. granulosus s.l. isolates from humans of neuquén provin ... | 2017 | 28721526 |
| genome-wide identification of microrna targets in the neglected disease pathogens of the genus echinococcus. | micrornas (mirnas), a class of small non-coding rnas, are key regulators of gene expression at post-transcriptional level and play essential roles in biological processes such as development. mirnas silence target mrnas by binding to complementary sequences in the 3'untranslated regions (3'utrs). the parasitic helminths of the genus echinococcus are the causative agents of echinococcosis, a zoonotic neglected disease. in previous work, we performed a comprehensive identification and characteriza ... | 2017 | 28385564 |