| isolation and structure of the cytotoxic cycloheptapeptide phakellistatin 13. | a new cyclic heptapeptide phakellistatin 13 (1) had been isolated from the sponge phakellia fusca thiele, collected at yongxing island of china. its structure was elucidated as cyclo-(pro1-trp-leu-thr-pro2-gly-phe) on the basis of ms, uv, ir, and high-field nmr (600 mhz) analysis. the compound was significantly cytotoxic against the human hepatoma bel-7404 cell line with an ed(50) < 10(-2) microg/ml. | 2003 | 12542366 |
| 5-fluorouracil derivatives from the sponge phakellia fusca. | 5-fluorouracil derivatives were isolated from the marine sponge phakellia fusca collected around the yongxing island of the xisha islands in the south sea of china. their structures were determined on the basis of spectral analysis and x-ray diffraction. | 2003 | 12608868 |
| quantitative determination of phakellistatin 13, a new cyclic heptapeptide, in rat plasma by liquid chromatography/tandem mass spectrometry: application to a pharmacokinetic study. | a sensitive liquid chromatography-tandem mass spectrometry (lc-ms/ms) method, followed by a 96-well protein precipitation, has been developed and fully validated for the determination of phakellistatin 13 (pk13), a new cyclic heptapeptide isolated from the sponge phakellia fusca thiele, in rat plasma. after protein precipitation of the plasma samples (50 microl) in a 96-well plate by methanol (200 microl) containing the internal standard pseudostellarin b (20 ng/ml), the plate was vortex mixed f ... | 2009 | 19760191 |
| modification of artificial sea water for the mass production of (+)-terrein by aspergillus terreus strain pf26 derived from marine sponge phakellia fusca. | (+)-terrein shows multiple bioactivities, however, its mass production is a big challenge. aspergillus terreus strain pf26 derived from south china sea sponge phakellia fusca has been cultured to produce (+)-terrein successfully, but artificial sea water (asw) of high salinity used in the fermentation medium may cause the corrosion risk of metal bioreactor, which limits the fermentation on a large scale. in this study, we modified the components of asw by removing nacl and cacl2 from the origina ... | 2015 | 26394071 |
| enhanced production of (+)-terrein in fed-batch cultivation of aspergillus terreus strain pf26 with sodium citrate. | (+)-terrein is a fungal metabolite with multiple biological activities, especially with great value in medicine. however, the mass production of single configuration terrein is still a big challenge. in this study, the effects of acetic acid, sodium acetate, citric acid and sodium citrate on the (+)-terrein production by aspergillus terreus strain pf26 derived from marine sponge phakellia fusca were investigated. sodium citrate was selected for fed-batch cultivation because it showed the best ef ... | 2013 | 23085955 |
| operating conditions optimization for (+)-terrein production in a stirred bioreactor by aspergillus terreus strain pf-26 from marine sponge phakellia fusca. | terrein is a fungal metabolite with application values in the fields of medicine, cosmetology, and agriculture. however, mass production of single configuration terrein is still a big challenge. in this study, operating factors such as inoculation, agitation speed, aeration rate, ph control, and nutrient feeding were preliminarily optimized to improve the (+)-terrein production in the 5-l stirred bioreactor from the marine sponge-derived fungus aspergillus terreus pf-26. spore inoculation, low a ... | 2012 | 22527032 |
| new metabolites and bioactive chlorinated benzophenone derivatives produced by a marine-derived fungus pestalotiopsis heterocornis. | four new compounds, including two isocoumarins, pestaloisocoumarins a and b (1, 2), one sesquiterpenoid degradation, isopolisin b (4), and one furan derivative, pestalotiol a (5), together with one known isocoumarin, gamahorin (3), and three chlorinated benzophenone derivatives, pestalachloride b (6), pestalachloride e (7) and a mixture of pestalalactone atropisomers (8a/8b), were isolated from a culture of the fungus pestalotiopsis heterocornis associated with sponge phakellia fusca. these new ... | 2017 | 28335391 |
| quinone/hydroquinone meroterpenoids with antitubercular and cytotoxic activities produced by the sponge-derived fungus gliomastix sp. zsds1-f7. | fifteen compounds, including six quinone/hydroquinone meroterpenoids, purpurogemutantin (1), macrophorin a (2), 4'-oxomacrophorin (3), 7-deacetoxyyanuthone a (4), 2,3-hydro-deacetoxyyanuthone a (5), 22-deacetylyanuthone a (6), anicequol (7), three roquefortine derivatives, roquefortine c (8), (16s)-hydroxyroquefortine c (9), (16r)-hydroxyroquefortine c (10), dihydroresorcylide (11), nectriapyrone (12), together with three fatty acid derivatives, methyl linoleate (13), phospholipase a2 (14), meth ... | 2017 | 27417331 |
| new furan and cyclopentenone derivatives from the sponge-associated fungus hypocrea koningii pf04. | two new furan derivatives, hypofurans a and b (1 and 2), and three new cyclopentenone derivatives, hypocrenones a-c (3-5), along with seven known compounds (6-12), were isolated from a marine fungus hypocrea koningii pf04 associated with the sponge phakellia fusca. among them, compounds 10 and 11 were obtained for the first time as natural products. the planar structures of compounds 1-5 were elucidated by analysis of their spectroscopic data. meanwhile, the absolute configuration of 1 was deter ... | 2015 | 26343687 |