| cloning and heterologous expression of ss10, a subtilisin-like protease displaying antifungal activity from trichoderma harzianum. | trichoderma harzianum parasitizes a large variety of phytopathogenic fungi. trichoderma harzianum mycoparasitic activity depends on the secretion of complex mixtures of hydrolytic enzymes able to degrade the host cell wall. a gene (ss10) encoding a subtilisin-like protease was cloned from t. harzianum t88, a biocontrol agent effective against soil-borne fungal pathogens. the full-length cdna was isolated by 5' and 3' rapid amplification of the cdna ends. the coding region of the gene is 1302 bp ... | 2009 | 19025577 |
| the chitinase gene (bbchit1) from beauveria bassiana enhances resistance to cytospora chrysosperma in populus tomentosa carr. | the chinese white poplar (populus tomentosa carr.) is susceptible to infection by plant diseases which severely affect its growth and substantially decrease its economic value. a chitinase gene (bbchit1) from beauveria bassiana was introduced into chinese white poplar (populus tomentosa carr.) by agrobacterium-mediated transformation. the t-dna of plant transformation vector contained the beta-glucuronidase reporter gene (gus) under the control of camv 35s promoter and the neomycin phosphotransf ... | 2010 | 20464449 |
| endophytic fungi from flowers, capsules and seeds of eucalyptus globulus. | the main goal of this work was to detect whether cytospora chrysosperma and fusicoccum eucalypti are present as endophytes of symptomless hypocotyls, cotyledons, flowers, capsules, peduncles of flowers in order to interpret an earlier finding of their presence in seeds, seedling stems and twigs of e. globulus. segments from these organs as well as from bark and the xylem from flower peduncles were surface-sterilized and plated on 2% malt-agar. all plates were incubated at 24 degrees c for six we ... | 2001 | 15482013 |