| detection and differentiation of ovine theileria and babesia by reverse line blotting in china. | a reverse line blot (rlb) assay was developed for detection and specific identification of the different ovine theileria and babesia parasites. in a polymerase chain reaction (pcr), the hypervariable region 4 (v4 region) of the 18s ribosomal dna gene was amplified with a set of general primers specific for members of the genera theileria and babesia. meanwhile, specific oligonucleotide probes were designed and bound on membrane. after one single-pcr amplification, the amplified fragment was hybr ... | 2009 | 19205742 |
| experimental transmission of theileria uilenbergi infective for small ruminants by haemaphysalis longicornis and haemaphysalis qinghaiensis. | the experimental transmission of a recently designated theileria uilenbergi pathogenic for sheep and goats in northern china is described. haemaphysalis qinghaiensis nymphs and adults developed from larvae and nymphs engorged on sheep infected with t. uilenbergi were able to respectively transmit the latter to sheep. however, when h. longicornis ticks picked up t. uilenbergi either at larval or nymphal, only the subsequent adult could transmit the parasites to sheep, the subsequent nymph could n ... | 2009 | 19198881 |
| establishment of optimal conditions for long-term culture of erythrocytic stages of theileria uilenbergi. | to establish optimal conditions for long-term culture of the erythrocytic stage of theileria uilenbergi. | 2006 | 17078754 |
| phylogenetic analysis of theileria species transmitted by haemaphysalis qinghaiensis. | the phylogenetic relationships between six isolates of theileria spp. infective to small ruminants, and two isolates of theileria spp. infective to yak, all transmitted by haemaphysalis qinghaiensis, together with the theileria orientalis/sergenti/buffeli group and t. sinensis, were analyzed using the 18s ssrrna gene sequence. the target dna segment was amplified by polymerase chain reaction (pcr). the pcr product was used either for direct sequencing or was ligated to the pcr ii vector for sequ ... | 2004 | 14598167 |
| identification of theileria uilenbergi immunodominant protein for development of an indirect elisa for diagnosis of ovine theileriosis. | theileriosis of small ruminants in the northwest of china is a protozoan disease that restricts the development of the livestock industry. the disease is caused by infection with theileria uilenbergi and theilerialuwenshuni, both of which are transmitted by ixodid heamaphysalis ticks. the development of serological tools as a means of integrated control of the disease is an urgent and important requirement. here we describe the identification and partial recombinant expression of a t.uilenbergi ... | 2010 | 19900458 |
| phylogenetic position of small-ruminant infecting piroplasms. | theileria and babesia are tick-transmitted protozoa that cause great economical losses in livestock. recently, interest has risen in sheep-infecting piroplasms and a number of previously unidentified pathogens were described, particularly in china. to address the phylogenetic relationship of theileria and babesia species infecting sheep, the complete sequences of the 18 s small subunit ribosomal rna genes of a panel of piroplasm isolates, including t. lestoquardi, t. ovis, t. separata, b. ovis, ... | 2006 | 17135558 |
| a study on ovine tick-borne hemoprotozoan parasites (theileria and babesia) in the east black sea region of turkey. | in this study, the frequency of theileria and babesia species was assessed via reverse line blotting and blood smear-based diagnostic methods in small ruminants. a total of 201 apparently healthy animals from 26 randomly selected herds located in 4 locations (artvin, giresun, gumushane, and tokat) of east black sea region of turkey were investigated for the blood protozoans. in a polymerase chain reaction (pcr), the hypervariable v4 region of the 18s ribosomal rna gene was amplified with a set o ... | 2012 | 22231266 |
| small-scale expressed sequence tag analysis of theileria uilenbergi: identification of a gene family encoding potential antigenic proteins. | recently, theileria sp. (china) has been designated as t. luwenshuni[formerly theileria sp. (china 1)] and t. uilenbergi[formerly theileria sp. (china 2)]. a cdna library of t. uilenbergi merozoites was constructed and subjected to random sequencing. among the obtained sequences were three highly identical cdna clones, indicating a gene family. bioinformatic analyses indicated these genes contain signal peptides and encode potential immunogenic proteins. the presence of tandemly arranged and add ... | 2008 | 19120214 |
| investigations into the natural infection rate of haemaphysalis qinghaiensis with piroplasma using a nested pcr. | here we describe the natural infection rate in china of haemaphysalis qinghaiensis with four piroplasma species, namely theileria uilenbergi, t. luwenshuni, t. sinensis and babesia motasi. specifically, a nested pcr was designed based on 18s ribosomal rna genes and its specificity and sensitivity were established. the result showed that 62 flat adult field h. qinghaiensis ticks (27 females and 35 males) out of 136 (55 females and 81 males) were infected by one or more parasites. all 62 (45.6%) w ... | 2008 | 18273685 |
| validation of a recombinant protein indirect elisa for the detection of specific antibodies against theileria uilenbergi and theileria luwenshuni in small ruminants. | an enzyme-linked immunosorbent assay (elisa) based on a recombinant theileria uilenbergi immunodominant protein (rtuip) was validated for detection of antibodies in 188 positive and 198 negative reference serum samples, respectively. the cut-off value was determined at 32.7% with 95% and 90% accuracy levels by two-graphic receiver-operating characteristic (tg-roc). the equal diagnostic sensitivity (se) and specificity (sp) were calculated to be 98.4%. further validation of the repeatability with ... | 2014 | 24912957 |
| an epidemiological survey of theileria infections in small ruminants in central china. | here, we conducted an epidemiological study in five regions in central china to assess the impact of theileriosis on small ruminants. pcr analysis and microscopic evaluations of blood smears to detect ovine and caprine theileriosis was conducted, in which 256 blood samples and 250 ticks were collected from sheep and goats, and tested for theileria uilenbergi, t. luwenshuni, and t. ovis. the 18s rrna gene sequences were deduced from positive samples and used for phylogenetic analysis. the results ... | 2014 | 24365241 |
| identification and characterization of tu88, an antigenic gene from theileria uilenbergi. | theileria uilenbergi is a pathogen that causes ovine theileriosis. prevention and control of theileriosis relies on its diagnosis at early stages of occurrence and requires understanding of proteins with antigenic properties from the pathogen. despite its prevalence in china, only a few molecules with antigenic properties have been characterized from t. uilenbergi. in this study, we identified a cdna named tu88 by immunoscreening a t. uilenbergi merozoite cdna library with t. uilenbergi-positive ... | 2015 | 25770598 |
| molecular evidence for piroplasms in wild reeves' muntjac (muntiacus reevesi) in china. | dna from liver samples of 17 free-ranging wild reeves' muntjac (muntiacus reevesi) was used for pcr amplification of piropalsm 18s rrna gene. of 17 samples, 14 (82.4%) showed a specific pcr product which were cloned and sequenced. blast analysis of the sequences obtained showed similarities to babesia sp., theileria capreoli, theileria uilenbergi and theileria sp. bo302-se. phylogenetic analysis showed that the babesia sp. detected in the present study was distantly separated from known babesia ... | 2014 | 24970769 |
| multiplex pcr for diagnosis of theileria uilenbergi, theileria luwenshuni, and theileria ovis in small ruminants. | infections with theileria sp. may cause significant economic losses to the sheep industry. species identification based on microscopic examination is difficult, and more suitable methods are required for the rapid detection and identification of theileria sp, in clinical specimens. in this study, a multiplex polymerase chain reaction (mpcr) assay was developed to simultaneously identify three individual theileria species in small ruminants. three pairs of specific, sensitive primers were designe ... | 2014 | 24241125 |
| an indirect elisa for detection of theileria spp. antibodies using a recombinant protein (rtlsp) from theileria luwenshuni. | theileria is a tick-borne, intracellular protozoan parasite of worldwide economic and veterinary importance in small ruminants. here, an enzyme-linked immunosorbent assay (elisa) was developed based on theileria luwenshuni recombinant surface protein (rtlsp) and was used in the standardization and validation of an elisa for the detection of circulating antibodies against ovine and caprine theileriosis. a total of 233 sera samples were used for the calculation of the cut-off value which served as ... | 2016 | 27048941 |
| comparative proteomic and bioinformatic analysis of theileria luwenshuni and theileria uilenbergi. | theileria is an obligatory intraerythrocytic protozoan parasite that causes economic losses to the cattle, sheep and goats industry. however, very little information is available on the genomes, transcriptomes, and proteomes of the ovine parasites, theileria luwenshuni and theileria uilenbergi. differences in protein expression between these species were investigated to better understand their biology. parasites were digested with trypsin, and the resulting peptides labeled with isobaric tags fo ... | 2016 | 27018062 |
| development of an immunochromatographic strip for the serodiagnosis of theileria infection in sheep. | theileria uilenbergi and t. luwenshuni are tick-borne protozoan parasites, transmitted by haemaphysalis qinghaiensis and h. longicornis, respectively. they are the main causative agents of theileriosis in small ruminants in china. the disease has resulted in severe economic losses and hindered the development of sheep and goat husbandry industry in the endemic regions. | 2015 | 26631226 |
| evaluating an indirect rmpsp enzyme-linked immunosorbent assay for the detection of bovine theileria infection in china. | bovine theileriosis, a tick-borne protozoan disease caused by theileria annulata, theileria orientalis and theileria sinensis, is widespread in china and is a serious economic problem for the chinese livestock industry. in this study, recombinant major piroplasma surface proteins (mpsp) of t. annulata, t. orientalis and t. sinensis based on mpsp genes were expressed in escherichia coli bl21(de3). the immunogenicity and specificity of the three purified recombinant mpsp proteins were evaluated wi ... | 2017 | 27942962 |
| molecular identification of theileria parasites of northwestern chinese cervidae. | theileria and babesia protozoan parasites are transmitted mainly by tick vectors. these parasites cause heavy economic losses to the live-stock industry, as well as affecting the health of wild animals in parasite-endemic areas. identification of infectious agents in wild animals is not only crucial for species preservation, but also provides valuable information on parasite epidemiology. here, we conducted a molecular surveillance study in northwestern china to assess the prevalence of blood pa ... | 2014 | 24885179 |
| seroprevalence of bovine theileriosis in northern china. | bovine theileriosis is a common disease transmitted by ticks, and can cause loss of beef and dairy cattle worldwide. here, an indirect enzyme-linked immunosorbent assay (ielisa) based on theileria luwenshuni surface protein (tlsp) was developed and used to carry out a seroepidemiological survey of bovine theileriosis in northern china. | 2016 | 27863521 |
| identification of clone-9 antigenic protein of theileria uilenbergi and evaluation of its application for serodiagnosis. | the pathogenic protozoan parasite theileria uilenbergi is one of the causative agents of theileriosis in small ruminants in china. the infection results in great economical losses in the northwest part of china. efforts are underway to establish an enzyme-linked immunosorbent assay (elisa) based on a t. uilenbergi immunodominant recombinantly expressed protein using different approaches in order to perform epidemiological studies in the area. in this study, we describe the possible use of the cl ... | 2010 | 20422216 |