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action of (2-->1)fructo-oligopolysaccharide fraction of chlorophytum borivilianum against streptozotocin-induced oxidative stress.a fructo-oligosaccharide was isolated from chlorophytum borivilianum and identified as o-beta-d-fructofuranosyl-(2-->1)-(beta-d-fructofuranosyl) (n)-(2-->1)-alpha-d-glucopyranoside (n = 5 - 30) using high-pressure anion exchange chromatography, maldi-ms, nmr, gc, hptlc and chemical analysis. the extract and the fructo-oligosaccharide were found to have significant antidiabetic activity with the blood sugar levels being 118.32 +/- 3.56 and 110.21 +/- 4.22, respectively, as compared to the control ...200617051465
ameliorative effect of chlorophytum borivilianum root on lipid metabolism in hyperlipaemic rats.1. the present study examined the efficacy of chlorophytum borivilianum root (powder) in modulating the hyperlipaemic/hypercholesteraemic conditions in male albino rats. 2. administration of c. borivilianum (0.75 and 1.5 g root powder/rat per day for 4 weeks) to hypercholesteraemic rats significantly increased high-density lipoprotein-cholesterol levels and decreased plasma and hepatic lipid profiles. 3. in addition, there were significant increases in faecal cholesterol, neutral sterol and bile ...200717250646
anti-stress and anti-oxidant effects of roots of chlorophytum borivilianum (santa pau & fernandes).the aqueous extract of c. borivilianum (250 mg/kg for 7 days) significantly reverted the elevated levels of plasma glucose, triglycerides, cholesterol and serum corticosterone and also reduced the ulcer index, adrenal gland weight more as effectively as the standard drug (diazepam) in rats. at 125 mg/kg po, it showed a mild anti-stress activity. under in vitro 1,1-diphenyl-2-picrylhydrazyl (dpph*) free radical scavenging assay and lipid peroxidation assay the extract considerably inhibited, in a ...200718072542
immunomodulatory activity of chlorophytum borivilianum sant. f.chlorophytum borivilianum santapau & fernandes (liliaceae) is a very popular herb in traditional indian medicine and constitute a group of herbs used as 'rasayan' or adaptogen. ethanolic extract of the roots and its sapogenin were evaluated for their immunomodulatory activity. effect of azathioprine-induced myelosuppresion and administration of extracts on hematological and serological parameters was determined. administration of extracts greatly improved survival against candida albicans infect ...200718227908
effects of chlorophytum borivilianum on sexual behaviour and sperm count in male rats.this study was designed to evaluate the aphrodisiac and spermatogenic potential of the aqueous extract of dried roots of chlorophytum borivilianum (cb) in rats. male wistar albino rats were divided into four groups. rats were orally treated with (1) control group: distilled water; (2) cb 125 mg/kg/day; (3) cb 250 mg/kg/day; and (4) viagra((r)) group: 4 mg/kg/day sildenafil citrate and their sexual behaviour was monitored 3 h later using a receptive female. their sexual behaviour was evaluated on ...200818412148
cytotoxic spirostane-type saponins from the roots of chlorophytum borivilianum.four new spirostane-type saponins named borivilianosides e-h (1-4) were isolated from an ethanol extract of the roots of chlorophytum borivilianum together with two known steroid saponins (5 and 6). the structures of 1-4 were elucidated using mainly 2d nmr spectroscopic techniques and mass spectrometry. the cytotoxicity of borivilianosides f (2), g (3), and h (4) and three known compounds was evaluated using two human colon cancer cell lines (ht-29 and hct 116).200919128156
a comparative study on aphrodisiac activity of some ayurvedic herbs in male albino rats.the roots of asparagus racemosus, chlorophytum borivilianum, and rhizomes of curculigo orchioides are popular for their aphrodisiac and immunostimulatory properties. the herbs have been traditionally used as vajikaran rasayana herbs because of their putative positive influence on sexual performance in humans. lyophilized aqueous extracts obtained from the roots of a. racemosus, c. borivilianum, and rhizomes of c. orchioides were studied for sexual behavior effects in male albino rats and compare ...200919139984
effect of chlorophytum borivilianum santapau and fernandes on sexual dysfunction in hyperglycemic male rats.to investigate the effects of chlorophytum borivilianum (cb) on sexual dysfunction, loss of body weight, and lack of libido in hyperglycemic rats induced with streptozotocin or alloxan.201020082251
antimicrobial activity of some indian medicinal plants.the antimicrobial potential of seventy-seven extracts from twenty-four plants was screened against eight bacteria and four pathogenic fungi, using microbroth dilution assay. lowest concentration of the extract, which inhibits any visual microbial growth after treatment with p-iodonitrotetrazolium violet, was considered to be minimum inhibitory concentration (mic). water extracts of acacia nilotica, justicia zelanica, lantana camara and saraca asoca exhibited good activity against all the bacteri ...200720161895
chlorophytum borivilianum as potential terminator of free radicals in various in vitro oxidation systems.chlorophytum borivilianum is a very popular herb in traditional indian medicine and used as a potent "rasayana" drug in "ayurveda" as a rejuvenator. currently, a large body of evidence supports the key role of free radicals in diverse pathological conditions such as aging and atherosclerosis. the present investigation essentially focuses on the comprehensive account of in vitro antioxidant activity exerted by c.borivilianum root extracts (i.e., aqueous and ethanolic), to clarify the pharmacologi ...201020307144
anti-tumour, anti-mutagenic and chemomodulatory potential of chlorophytum borivilianum.in the present investigation anti-tumour, anti-mutagenic and chemomodulatory potential of chlorophytum borivilianum were evaluated. chlorophytum borivilianum root extract had no toxic effect up to a dose of 800 mg/kg body weight/day. significant increase (p<0.05 to p<0.001) in the activity of reduced glutathione (gsh), catalase (cat) and superoxide dismutase (sod) and a significant decrease in the hepatic mda level were observed at 100, 400, and 800 mg/kg body weight of chlorophytum borivilianum ...201020843110
improvement of penile erection, sperm count and seminal fructose levels in vivo and nitric oxide release in vitro by ayurvedic herbs.in the present study, the effect of four vajikaran rasayana herbs on penile erection, sperm count, seminal fructose content in vivo and nitric oxide (no) release in vitro was assessed. lyophilised aqueous extracts of asparagus racemosus willd. (ar), chlorophytum borivilianum sant. f. (cb), curculigo orchioides gaertn. (co), and dactylorhiza hatagirea (d. don) soo (dh) were orally administered at 100 mg/kg body weight to wistar strain male albino rats. penile erection index and sperm count were d ...201121486409
immunomodulatory polysaccharide from chlorophytum borivilianum roots.chlorophytum borivilianum santapau & fernandes (liliaceae) is an ayurvedic rasayana herb with immunostimulating properties. the polysaccharide fraction (cbp) derived from hot water extraction of c. borivilianum (cb), comprising of ~31% inulin-type fructans and ~25% acetylated mannans (of hot water-soluble extract), was evaluated for its effect on natural killer (nk) cell activity (in vitro). human peripheral blood mononuclear cells (pbmcs), isolated from whole blood on a ficoll-hypaque density g ...201121792363
ameliorative effects of herbal combinations in hyperlipidemia.the roots of glycyrrhiza glabra, withania somnifera, asparagus racemosus, and chlorophytum borivilianum and seeds of sesamum indicum are ayurvedic medicinal plants used in india to treat several ailments. our previous studies indicated that these plants possess hypolipidemic and antioxidant potential. the present study was aimed at investigating the composite effects of these plants on hypercholesterolemic rats. three different combinations (5 gm%, given for four weeks) used in this study effect ...201121941605
plant regeneration in chlorophytum borivilianum sant. et fernand. from embryogenic callus and cell suspension culture and assessment of genetic fidelity of plants derived through somatic embryogenesis.efficient in vitro propagation of medicinally important endangered plant c. borivilianum has been achieved through somatic embryogenesis. solid embryogenic medium [murashige and skoog medium containing 1.79 mm nh4no3, 10.72 mm kno3, 1.13 μm 2,4-dichlorophenoxyacetic acid, 7.38 μm 2-isopentenyladenine and 0.76 mm proline] supplemented with polyethylene glycol and sucrose (3 % each), exhibited 1.88-fold increase in embryo maturation compared to embryogenic medium containing 3 % sucrose. liquid emb ...201223814440
lack of genetic diversity of a critically endangered important medicinal plant chlorophytum borivilianum in central india revealed by aflp markers.chlorophytum borivilianum is a traditional medicinal plant distributed throughout the tropics and subtropics. in the present investigation, aflp analysis was used to assess the genetic similarity among 34 accessions. nine primer sets of aflp amplified 612 fragments, of which 246 fragments were found to be polymorphic. the average number of polymorphic bands per aflp primer pair was 27.33. the amplified fragments ranged from 50 base pairs to 600 base pairs. significant correlation was observed be ...201223573053
chlorophytum borivilianum polysaccharide fraction provokes the immune function and disease resistance of labeo rohita against aeromonas hydrophila.the present study aimed to investigate the effects of chlorophytum borivilianum polysaccharide (cbp), as a dietary supplement administered at varying concentrations with feed (basal diet), on various cytokine-related responses in labeo rohita fingerlings. immune parameters and immune-related gene expressions were measured at 3rd, 4th, and 5th week after feeding. the results revealed that dietary administration of cbp at 0.2% and 0.4% for 4 weeks significantly upregulated serum lysozyme and phago ...201526649325
characterization of squalene synthase gene from chlorophytum borivilianum (sant. and fernand.).saponins are important group of secondary metabolites known for their pharmacological properties. chlorophytum borivilianum contains high amount of saponins and is thus, recognized as an important medicinal plant with aphrodisiac properties. though the plant is well known for its pharmaceutical properties, there is meager information available about the genes and enzymes responsible for biosynthesis of saponins from this plant. squalene synthase (sqs) is the key enzyme of saponin biosynthesis pa ...201323338982
an improved method for thin layer chromatographic analysis of saponins.analysis of saponins by thin layer chromatography (tlc) is reported. the solvent system was n-butanol:water:acetic acid (84:14:7). detection of saponins on the tlc plates after development and air-drying was done by immersion in a suspension of sheep erythrocytes, followed by washing off the excess blood on the plate surface. saponins appeared as white spots against a pink background. the protocol provided specific detection of saponins in the saponins enriched extracts from aesculusindica (wall ...201226434348
micropropagation: a tool for the production of high quality plant-based medicines.medicinal plants are the most important source of life saving drugs for the majority of the world's population. the biotechnological tools are important to select, multiply and conserve the critical genotypes of medicinal plants. plant tissue culture techniques offer an integrated approach for the production of standardized quality phytopharmaceutical through mass-production of consistent plant material for physiological characterization and analysis of active ingredients. micropropagation proto ...200616472132
inventorization of some ayurvedic plants and their ethnomedicinal use in kakrajhore forest area of west bengal.medicinal plant resources of forest origin are extensively used in india for various systems of medicine like ayurveda, unani, homeopathy, allopathy, siddha and ethnic etc. the tribal communities around the kakrajhore forest in west medinipur district of west bengal have their own traditional knowledge based system of curing many diseases using the forest based plant resources similar to ayurveda. the forest comprises of one of the unique treasure and rich source of diversified ethno-botanical w ...201727590732
induction, subculture cycle, and regeneration of callus in safed musli (chlorophytum borivilianum) using different types of phytohormones.chlorophytum borivilianum is an industrially valued medicinal crop. propagation through seeds is not feasible because of low germination percentage and long dormancy period. therefore, callus culture and plant regeneration can be an alternative to improve this crop production. also, callus can serve as an alternative source of bioactive compounds.201627761075
an evidence-based approach to medicinal plants for the treatment of sperm abnormalities in traditional persian medicine.infertility is defined as inability of a sexually active couple to conceive after 1 year of regular intercourse without contraception. male factors account for 20%-50% of cases of infertility. the aim of this study was to review medicinal plants that proposed to improve sperm abnormalities in traditional persian medicine. for this purpose, pubmed, scopus, googlescholar and cochrane library were explored for medicinal plants used in traditional persian medicine for sperm abnormalities to obtain s ...201627681644
standardised extract of safed musli (chlorophytum borivilianum) increases aphrodisiac potential besides being safe in male wistar rats.the standardised extract of root of safed musli (chlorophytum borivilianum) was evaluated for its aphrodisiac potential and safety profile on reproductive system. wistar albino rats were trained to provide sexual experience under a dim red light (10 w) in a glass tank. male and female rats were placed periodically in the glass tank in a particular order, that is male followed by introduction of the receptive female. dosing of extract was carried out for 54 days at 125 and 250 mg kg(-1) p.o to ma ...201626952773
retraction note to: plant regeneration in chlorophytum borivilianum sant. et fernand. from embryogenic callus and cell suspension culture and assessment of genetic fidelity of plants derived through somatic embryogenesis.[this retracts the article doi: 10.1007/s12298-012-0113-y.].201526600689
analysis of genetic variability in endemic medicinal plants of genus chlorophytum from the indian subcontinent using amplified fragment length polymorphism marker.the genus chlorophytum consists of medicinally important species like chlorophytum borivilianum, c. tuberosum and c. attenuatum. uncontrolled harvest of this plant from wild habitat due to its high commercial value made the species of this genus be listed in the red data book of indian plants as an endangered species. in india, approximately nineteen species of chlorophytum are found; out of these, only c. borivilianum is cultivated commercially. the objective of this study was to measure geneti ...201526573876
rna-seq mediated root transcriptome analysis of chlorophytum borivilianum for identification of genes involved in saponin biosynthesis.chlorophytum borivilianum is an important species of liliaceae family, owing to its vital medicinal properties. plant roots are used for aphrodisiac, adaptogen, anti-aging, health-restorative and health-promoting purposes. saponins, are considered to be the principal bioactive components responsible for the wide variety of pharmacological properties of this plant. in the present study, we have performed de novo root transcriptome sequencing of c. borivilianum using illumina hiseq 2000 platform, ...201626458557
saponins isolated from roots of chlorophytum borivilianum reduce acute and chronic inflammation and histone deacetylase.the roots of chlorophytum borivilanum are used in traditional medicine for the treatment of arthritis and inflammation. the aim of the work was to evaluate the anti-inflammatory activity of isolated saponins from chlorophytum borivilianum (iscb).201525609369
chlorophytum borivilianum root extract maintains near normal blood glucose, insulin and lipid profile levels and prevents oxidative stress in the pancreas of streptozotocin-induced adult male diabetic rats.the effect of c. borivilianum root on blood glucose, glycated hemoglobin (hbaic), insulin and lipid profile levels in diabetes mellitus are not fully understood. this study therefore investigated the effect of c. borivilianum root on the above parameters and oxidative stress of the pancreas in diabetes.201425249786
chlorophytum borivilianum (safed musli) root extract prevents impairment in characteristics and elevation of oxidative stress in sperm of streptozotocin-induced adult male diabetic wistar rats.we hypothesized that c. borivilianum root, known to improve male reproductive performance, prevents impairment in characteristics, morphology and elevation of oxidative stress in sperm of diabetics. we therefore investigated the effect of aqueous root extract of c. borivilianum on these parameters in diabetic rat model.201425104050
potential regulatory role of gibberellic and humic acids in sprouting of chlorophytum borivilianum tubers.tubers of safed musli (chlorophytum borivilianum) were immersed in three different concentrations of gibberellic acid (ga3) or humic acid (ha) prior to planting. the highest concentration of ga3 (20 mg l(-1)) and all concentrations of ha (5, 10, and 15%) appeared to hasten tuber sprouting and promote uniform sprouting pattern. the use of 20 mg l(-1) ga3 or 15% ha successfully improved sprouting and mean sprouting time. safed musli growth and development was improved through the increase in the n ...201424688363
clinical evaluation of root tubers of shweta musali (chlorophytum borivilianum l.) and its effect on semen and testosterone.shweta musali (chlorophytum borivilianum (cb)) is a traditionally used herb for its benefits in male sexual and general health. in the recent past, the herb has attained much commercial significance, both in domestic and international markets. however, limited clinical data is available to establish its traditional claims. the present study was aimed at evaluating the effect of the water soluble extract of cb root tubers on semen and testosterone in healthy adult males. the research was designed ...201324501522
de novo transcriptome sequencing reveals important molecular networks and metabolic pathways of the plant, chlorophytum borivilianum.chlorophytum borivilianum, an endangered medicinal plant species is highly recognized for its aphrodisiac properties provided by saponins present in the plant. the transcriptome information of this species is limited and only few hundred expressed sequence tags (ests) are available in the public databases. to gain molecular insight of this plant, high throughput transcriptome sequencing of leaf rna was carried out using illumina's hiseq 2000 sequencing platform. a total of 22,161,444 single end ...201324376689
assessment of antioxidant and cytotoxicity activities of saponin and crude extracts of chlorophytum borivilianum.the present paper focused on antioxidant and cytotoxicity assessment of crude and total saponin fraction of chlorophytum borivilianum as an important medicinal plant. in this study, three different antioxidant activities (2,2-diphenyl-1-picrylhydrazyl radical scavenging (dpph), ferrous ion chelating (fic), and β -carotene bleaching (bcb) activity) of crude extract and total saponin fraction of c. borivilianum tubers were performed. crude extract was found to possess higher free radical scavengin ...201324223502
safed musli (chlorophytum borivilianum): a review of its botany, ethnopharmacology and phytochemistry.safed musli (chlorophytum borivilianum) is an eminent medicinal plant of india and considered as a 'white gold' or 'divya aushad' in indian systems of medicine. in ayurveda, chlorophytum borivilianum belongs to the group of "vajikaran rasayana" corroborated to its rejuvenating, aphrodisiac, natural sex tonic properties and effective in alleviating sexual disorders. it is largely used as ethnic medicine by local healers of indigenous communities of india.201324045177
bioefficacy of hydromethanolic extract of tuber of chlorophytum borivilianum (safed musli) for the management of male infertility in cyproterone acetate-treated albino rats.increase in male sexual dysfunction, and its treatment with conventional aphrodisiac drugs with side effects lead to investigate the spermatogenesis and androgenesis augmentative efficacy of hydromethanolic (40 : 60) extract of root of chlorophytum borivilianum (family - liliaceae) against cyproterone acetate-induced subfertility in wistar strain male albino rat. for this purpose, experimental rats were divided into three treatment groups: vehicle (received distilled water), cyproterone acetate ...201423957231
in vitro tuberization of chlorophytum borivilianum sant & fern (safed musli) as influenced by sucrose, ccc and culture systems.this study focuses on the establishment of in vitro tuberization of chlorophytum borivilianum using solid and liquid culture systems. a high in vitro tuberization rate on solid and stationary liquid murashige and skoog media was observed in the presence of 60 g l⁻¹ sucrose with 950, 1,265 and 1,580 µm 2-chloroethyl-trimethylammonium chloride (ccc). application of a higher sucrose concentration of 90 g l⁻¹ showed a negative interaction with ccc on in vitro tuber number and days to in vitro tuber ...201323749812
screening for rho-kinase 2 inhibitory potential of indian medicinal plants used in management of erectile dysfunction.activation of rho-kinase 2 (rock-ii) results in contraction of corpus cavernosum smooth muscle and rock-ii inhibitors relax corpus cavernosum in vitro and in vivo hence, plant extracts capable of inhibiting rock-ii enzyme may be useful in management of erectile dysfunction (ed). the aim of the study was to screen selected indian medicinal plants, having similar ethnopharmacological use for rock-ii inhibition.201223043981
differentially expressed transcripts from leaf and root tissue of chlorophytum borivilianum: a plant with high medicinal value.chlorophytum borivilianum is one of the important medicinal plants used for treating different health problems such as diabetes, arthritis, physical weakness, etc. saponins present in c. borivilianum are the primary source of its significant medicinal properties and are synthesized by mevalonate and non-mevalonate pathways in plants. however, the biosynthesis of these compounds at molecular level is not studied in c. borivilianum. cloning and sequencing of genes involved in metabolic processes a ...201223000016
a dietary supplement containing chlorophytum borivilianum and velvet bean improves sleep quality in men and women.impaired sleep quality is commonplace within industrialized societies, as evidenced by the increasing number of prescription sleep aids available. certain herbal preparations have been suggested to provide a natural benefit to sleep; however, limited controlled data are available documenting this benefit. in the present study we tested the effect of an experimental dietary supplement, containing the active ingredients chlorophytum borivilianum and velvet bean, on sleep quality using the pittsbur ...201222778558
antioxidant and modulatory role of chlorophytum borivilianum against arsenic induced testicular impairment.arsenic has a suppressive influence on spermatogenesis and induces impairment in male reproductive system due to oxidative stress. the present study was aimed to test the arsenic induced toxicity and protection by chlorophytum borivilianum. the effect of sodium arsenite (4 mg/(kg body weight (bw) x day)) via double distilled water without or with c. borivilianum (800 mg/(kg bw x day)) was evaluated in swiss albino mice for 30 days. the radical scavenging activity of the aqueous c. borivilianum r ...201223534213
isolation and characterization of phytoconstituents from chlorophytum borivilianum.the present communication deals with the identification and characterization of bioactive principles from the roots of chlorophytum borivilianum.201021713136
a blend of chlorophytum borivilianum and velvet bean increases serum growth hormone in exercise-trained men.several isolated ingredients have been proposed to increase growth hormone (gh) release, including chlorophytum borivilianum and velvet bean. a combination of these two ingredients has been packaged within an investigational dietary supplement. it was the purpose of the present investigation to determine the impact of acute ingestion of this supplement on circulating gh in healthy, exercise-trained men.201123946662
in vitro propagation of an endangered medicinal herb chlorophytum borivilianum sant. et fernand. through somatic embryogenesis.tuberous roots of chlorophytum borivilianum sant. et fernand. which are a source of steroidal saponins, possess immunomodulatory, adaptogenic, aphrodisiac, antipyretic, diuretic, hemostatic and anti-tumour properties. poor seed setting and germination and slow growth in conventional vegetative propagation are major constraints in the large-scale cultivation of this commercially important medicinal plant. in the present study, a procedure for in vitro propagation of this endangered herb through s ...201023572975
chlorophytum borivilianum: a white gold for biopharmaceuticals and neutraceuticals.chlorophytum borivilianum santapau & fernandes (liliaceae) also known as 'safed musli' is a traditional rare indian medicinal herb which has many therapeutic applications in ayurvedic, unani, homeopathic and allopathic system of medicine. its roots (tubers) are widely used for various therapeutic applications. it is used to cure physical illness and weakness, as an aphrodisiac agent and revitalizer, as general sex tonic, remedy for diabetes, arthritis and increasing body immunity, curative for n ...200919751181
reduction of vitrification in in vitro raised shoots of chlorophytum borivilianum sant. & fernand., a rare potent medicinal herb.reduction of vitrification in in vitro raised shoots derived from shoot bases and immature floral buds along with inflorescence axis used as explants of c. borivilianum, a rare medicinal herb is described. shoot multiplication was obtained on ms medium with 2 mg l(-1) benzylaminopurine (bap) + 0.1 mg l(-1) indole-3-butyric acid (iba) and ms medium with 2 mg l(-1) kinetin (kin) + 0.1 mg l(-1) 2,4-dichlorophenoxy acetic acid (2,4-d) from shoot bases and inflorescence axis respectively. best multip ...200616784122
in vitro clonal propagation of chlorophytum borivilianum sant. et fernand., a rare medicinal herb from immature floral buds along with inflorescence axis.a novel method of shoot regeneration from immature floral buds along with inflorescence axis in c. borivilianum, a rare medicinal herb is described. using this explant, axenic cultures were established with very less contamination (10%). ms medium with 2 mg l(-1) kinetin and 0.1 mg l(-1) 2, 4-dichlorophenoxyacetic acid proved to be the best for multiple shoot induction. maximum number (35) of shoot production was achieved in ms medium with 2 mg l(-1) benzylaminopurine. rooting of shoots (86.7%) ...200616430096
pharmacognostical evaluation of chlorophytum borivilianum root.chlorophytum borivilianum santapan and fernandes is an important medicinal plant in ayurveda and it is used mainly for its aphrodisiac activity. it is very closely related to other species also. the present work attempts to identify the chlorophytum borivilianum from other allied species in general by pharmacognostical, preliminary phytochemical and fluorescence analysis methods.200422557148
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