| lasiodiplodia theobromae as a cause of keratomycoses. | four cases of human keratitis caused by the tropical fungus lasiodiplodia theobromae have been encountered in miami, florida bringing to 8 the number of cases reported in the world literature. two of the ulcers were mild. three patients recovered without severe impairment of vision after topical polyene treatment, but 1 patient with a severe ulcer required therapeutic keratoplasty after 11 days of topical natamycin. histopathology revealed fungus deep in the cornea, invading descemet's membrane. ... | 1976 | 1085494 |
| intrahyphal hyphae in corneal tissue from a case of keratitis due to lasiodiplodia theobromae. | lasiodiplodia theobromae, a recognized plant pathogen, was isolated in culture from a case of human mycotic keratitis. chemotherapy with a variety of azoles was unsuccessful and the lesion was removed surgically. electron microscopy of thin sections of the excised corneal tissue revealed several examples of intrahyphal hyphae, a unique process described previously in in vitro cultures of various zoopathogenic fungi. we believe this to be the first report of the presence of intrahyphal hyphae in ... | 1991 | 1941432 |
| microbial transformations of some monoterpenoids and sesquiterpenoids. | the absolute configurations of fragrances, flavours and drugs are often important for their special properties. the growing interest of organic chemists in chiral synthons has stimulated work on biotransformations, for which readily available and inexpensive compounds can be used as substrates. microbial transformations of 1-menthenes like gamma-terpinene, alpha-terpinene, limonene and alpha-phellandrene give the corresponding 1,2-trans-diols with high stereospecificity. because of the volatilit ... | 1985 | 3848377 |
| lasiodiplodia theobromae panophthalmitis. | lasiodiplodia theobromae is a rare ocular pathogen. we report a case of panophthalmitis caused by this fungus. the patient was a healthy 62-year-old janitor with no history of ocular trauma in whom keratitis developed. cultures of corneal scrapings identified the fungus. despite treatment with appropriate antimycotics the keratitis progressed to infectious scleritis and then spontaneous perforation of the globe, which necessitated enucleation. the ultrasonographic, microbiologic and histologic f ... | 1985 | 3904960 |
| mycotoxicological investigations on maize and groundnuts from the endemic area of mseleni joint disease in kwazulu. | samples of home-grown maize and groundnuts from the endemic area of mseleni joint disease (mjd) during four seasons (1980-1983) were examined mycologically. the mycoflora of these dietary staples included fusarium poae and f. oxysporum, which have been implicated in the aetiology of kashin-beck or urov disease, another osteo-arthrosis endemic in siberia and china. two other species of fusarium, i.e. f. moniliforme and f. equiseti, implicated in syndromes of abnormal bone development in animals, ... | 1986 | 3961620 |
| the normal mycoflora of commodities from thailand. 2. beans, rice, small grains and other commodities. | part of a comprehensive study of fungi occurring in commodities in thailand, this paper reports results from 276 samples of mung beans, rice, sorghum and soybeans as well as other minor crops. samples for major commodities were taken from farmers' stocks, middlemen and retail outlets, while those for minor crops were mostly from retail. each sample was divided into two portions, one being examined in bangkok and the second at north ryde. mycological examinations were carried out by direct platin ... | 1994 | 7811571 |
| survey of mycotic and bacterial keratitis in sri lanka. | over a two-year period (1976-1977 and 1980-1981), 66 cases of bacterial and mycotic cases of keratitis were diagnosed in the eye clinic of the general hospital in kandy, sri lanka. the clinical and microbiologic aspects of these cases are described. noteworthy was the first known human case caused by paecilomyces farinosus, a geophilic species, commonly encountered as an insect parasite throughout the world. the bacterial and the other fungal etiologic agents isolated and identified were: pseudo ... | 1994 | 7984216 |
| the normal mycoflora of commodities from thailand. 1. nuts and oilseeds. | a comprehensive study was carried out of the fungi occurring in commodities normally traded in thailand. samples of major commodities were obtained from farmers' stocks and middlemen in major producing areas throughout the country. retail samples were obtained from outlets in and around bankok. samples were divided into two portions, one being examined in bangkok, and the second in sydney. after surface disinfection, fungi were enumerated by direct plating on dichloran rose bengal chloramphenico ... | 1993 | 8110599 |
| lasiodiplodia theobromae isolated from a subcutaneous abscess in a cambodian immigrant to australia. | a patient from cambodia presented at a hospital in melbourne with a persistent subcutaneous abscess of the right buttock. septate fungal hyphae were seen in biopsy tissue. lasiodiplodia theobromae was isolated and identified by the formation of pycnidia that produced typical conidia. the abscess was managed by drainage and debridement only. we briefly review the history and importance of the fungus as a wound parasite in tropical horticulture and its relatively rare occurrence in human infection ... | 1996 | 8873888 |
| endophthalmitis after lasiodiplodia theobromae corneal abscess. | lasiodiplodia theobromae is an exceptional cause of human keratomycosis. | 1997 | 9143896 |
| potato micro-tuber inducing hydroxylasiodiplodins from lasiodiplodia theobromae. | three hydroxylasiodiplodins were isolated from the mycelium extracts of lasiodiplodia theobromae ifo 31059; and their structures were identified as (3r),(4s)-4-hydroxylasiodiplodin, (3r),(6r)-6-hydroxy-de-o-methyllasiodiplodin and (3r),(5r)-5-hydroxy-de-o-methyllasiodiplodin. all showed potato micro-tuber inducing activity. | 2000 | 10939352 |
| fungicidal activity of essential oils of cinnamomum zeylanicum (l.) and syzygium aromaticum (l.) merr et l.m. perry against crown rot and anthracnose pathogens isolated from banana. | to develop a post-harvest treatment system against post-harvest fungal pathogens of banana using natural products. | 2002 | 12180942 |
| current perspectives on ophthalmic mycoses. | fungi may infect the cornea, orbit and other ocular structures. species of fusarium, aspergillus, candida, dematiaceous fungi, and scedosporium predominate. diagnosis is aided by recognition of typical clinical features and by direct microscopic detection of fungi in scrapes, biopsy specimens, and other samples. culture confirms the diagnosis. histopathological, immunohistochemical, or dna-based tests may also be needed. pathogenesis involves agent (invasiveness, toxigenicity) and host factors. ... | 2003 | 14557297 |
| fungal pathogens associated with banana fruit in sri lanka, and their treatment with essential oils. | the crown rot pathogens isolated from banana samples collected from 12 localities in sri lanka were lasiodiplodia theobromae, fusarium proliferatum and colletotrichum musae. fungal pathogens isolated were able to cause crown rot disease alone or in combination. disease severity was higher when combinations of virulent pathogens were used. cymbopogon nardus and ocimum basilicum oils displayed fungicidal activity against c. musae and f. proliferatum between 0.2-0.6% (v/v) in a poisoned food bioass ... | 2004 | 15008351 |
| a new antifungal metabolite from penicillium expansum. | a new antifungal compound, (3s)-4,6-dihydro-8-methoxy-3,5-dimethyl-6-oxo-3h-2-benzopyran (4), was isolated from penicillium expansum. during the isolation procedure 4 was determined to be unstable and readily reacted with methanol, ethanol, and water, forming three new isochromans, (1s,3s)-6-hydroxy-1,8-dimethoxy-3,5-dimethylisochroman (1), 1-ethoxy-6-hydroxy-8-methoxy-3,5-dimethylisochroman (2), and 1,6-dihydroxy-8-methoxy-3,5-dimethylisochroman (3), respectively. (3s)-6-hydroxy-8-methoxy-3,5-d ... | 2004 | 15270557 |
| culture filtrate of lasiodiplodia theobromae restricts the development of natural resistance in brassica nigra plants. | culture filtrate of lasiodiplodia theobromae increased respiration rate, phenylalanine ammonia lyase activity, and levels of hydrogen peroxide, lipid peroxides and salicylic acid in b. nigra plants. salicylic acid (sa) level increased for 1 hr of interaction and reduced later. development of systemic acquired resistance (sar) was found restricted in plants infected with l. theobromae due to deficiency of sa, which is a major signal for development of sar. exogenously supplied sa did develop resi ... | 2004 | 15274491 |
| isolation of an alpha-methylene-gamma-butyrolactone derivative, a toxin from the plant pathogen lasiodiplodia theobromae. | lasiodiplodia theobromae is known as a multi-infectious microorganism that causes considerable crop damage, particularly to tropical fruits. when the fruits are infected by l. theobromae, the typical symptom is the appearance of black spots on the surface of the infected fruit. when injected in to the peel of banana, the culture filtrate of l. theobromae induced formation of black spots. the structure of the isolated compound responsible for this effect was determined to be (3s,4r)-3-carboxy-2-m ... | 2004 | 15474567 |
| subcutaneous phaeohyphomycosis caused by lasiodiplodia theobromae and successfully treated surgically. | while visiting jamaica, a 50-year-old woman stumbled on an outdoor wooden staircase and sustained an injury to the right leg. the wound was cleaned topically and the patient was given antibacterial therapy. five weeks later, in canada, she presented with an ulcer at the injury site. an excisional biopsy showed copious broad, septate, melanized fungal filaments penetrating into tissue. culture yielded a nonsporulating melanized mycelium. the isolate was strongly inhibited by cycloheximide and ben ... | 2004 | 15682643 |
| antimicrobial and insecticidal protein isolated from seeds of clitoria ternatea, a tropical forage legume. | the tropical forage legume clitoria ternatea (l.) has important agronomic traits such as adaptation to a wide range of soil conditions and resistance to drought. it is resistant to a number of pathogens and pests. these important traits gave us reasons to look more closely at the plant. a highly basic small protein was purified from seeds of c. ternatea to homogeneity by using ultrafiltration with centricon-3 membrane tubes and preparative granulated-bed isoelectric focusing (ief). a single prot ... | 2004 | 15694280 |
| levels of common antigens in determining pathogenicity of curvularia eragrostidis in different tea varieties. | pathogenicity of curvularia eragrostidis, a foliar fungal pathogen of tea was studied in 24 commercially cultivated tea varieties by analysing the antigenic patterns of host and pathogen with the help of immunoserological techniques. | 2005 | 15836477 |
| novel potato micro-tuber-inducing compound, (3r,6s)-6-hydroxylasiodiplodin, from a strain of lasiodiplodia theobromae. | a novel potato micro-tuber-inducing compound was isolated from the culture broth of lasiodiplodia theobromae shimokita 2. the structure of the isolated compound was determined as (3r,6s)-6-hydroxylasiodiplodin by means of spectroscopic analyses, the modified mosher method, and chemical conversion. the compound showed potato micro-tuber-inducing activity at a concentration of 10(-4) m, using the culture of single-node segments of potato stems in vitro. | 2005 | 16116294 |
| microbial 7alpha-hydroxylation of 3-ketobisnorcholenol. | the transformation of 22-hydroxy-23,24-bisnorchol-4-en-3-one to 7alpha-22-dihydroxy-23,24-bisnorchol-4-en-3-one by botryodiploida theobromae, lasiodiplodia theobromae, and various botryosphaeria strains is described. factors affecting the reaction were incubation temperature, sonication of the substrate, and addition of 2,2'-dipyridyl, extra carbohydrate, and amberlite xad-7. the enzyme responsible for the reaction appeared to be very specific and was not characteristic of all members of the gen ... | 1986 | 16347069 |
| ceratocystis omanensis, a new species from diseased mango trees in oman. | mango (mangifera indica) sudden decline is an important disease in oman, which is closely associated with infections by ceratocystis fimbriata and lasiodiplodia theobromae. another ceratocystis species has also been found associated with symptoms on diseased trees. in this study, we identify that ceratocystis based on morphology and dna sequences. morphological comparisons showed that the fungus from dying mango trees in oman is similar to c. moniliformis. both fungi have distinct hat-shaped asc ... | 2006 | 16388939 |
| [mycotic keratitis and endophthalmitis caused by unusual fungi: lasiodiplodia theobromae]. | we report a case of endophthalmitis caused by the fungus lasiodiplodia theobromae. a 68-year-old man was referred to the hospital for right ocular pain since experiencing right ocular branch trauma 2 weeks before. the best-corrected acuity was limited to hand motion. slit-lamp examination showed a large corneal abscess and an anterior chamber reaction. the patient underwent systemic and local antibiotic therapy, and corneal scraping for microbiological diagnosis. sabouraud-chloramphenicol-gentam ... | 2006 | 16523150 |
| three new lasiodiplodia spp. from the tropics, recognized based on dna sequence comparisons and morphology. | botryosphaeria rhodina (anamorph lasiodiplodia theobromae) is a common endophyte and opportunistic pathogen on more than 500 tree species in the tropics and subtropics. during routine disease surveys of plantations in australia and venezuela several isolates differing from l. theobromae were identified and subsequently characterized based upon morphology and its and ef1-alpha nucleotide sequences. these isolates grouped into three strongly supported clades related to but different from the known ... | 2006 | 17040071 |
| biosynthesis of theobroxide and its related compounds, metabolites of lasiodiplodia theobromae. | administration of (13)c labeled acetates ([1-(13)c], [2-(13)c] and [1,2-(13)c(2)] to lasiodiplodia theobromae showed the tetraketide origins of both theobroxide, a potato-tuber inducing substance [1, (1s, 2r, 5s, 6r)-3-methyl-7-oxa-bicyclo[4.1.0]hept-3-en-2,5-diol]) and its carbonyldioxy derivative [2, (1s, 4r, 5s, 6r)-7,9-dioxa-3-methyl-8-oxobicyclo [4.3.0]-2-nonene-4,5-diol]. the incorporation of acetate-derived hydrogen into 1 and 2 was studied using [2-(2)h(3), 2-(13)c]acetate. three and one ... | 2007 | 17258244 |
| synthesis, spectroscopic and biological aspects of iron(ii) complexes. | five novel coordinated complexes of iron(ii) with ciprofloxacin and neutral bidentate ligands have been prepared and characterized using elemental analyses, magnetic measurements, ir spectra, uv-vis spectral, thermogravimetric analyses, 1h-nmr and 13c-nmr. the antimicrobial activity of the individual ligands, metal salt and metal complexes with respect to bacillus subtilis, escherichia coli, bacillus cereus, staphylococcus aureus, salmonella typhi, serratia marcescens, aspergillus niger, aspergi ... | 2007 | 17847716 |
| lasiodiplodia theobromae pneumonia in a liver transplant recipient. | we report a case of lasiodiplodia theobromae pneumonia in a patient who died 14 days after cadaveric-liver transplantation. his condition was complicated by enterococcus faecium peritonitis. direct microscopy analysis of the bronchoalveolar lavage specimens showed septate hyphae. a dematiaceous mold was recovered and identified as l. theobromae by microscopic morphology and ef1alpha gene sequencing. | 2008 | 18003802 |
| iron(iii) complexes: preparation, characterization, antibacterial activity and dna-binding. | iron(iii) have been combined to well known quinolones (ciprofloxacin) and some schiff bases with the help of coordination approach. characterization of these compounds have been done using elemental analysis, magnetic measurements, thermogravimetric analysis, ir, uv-vis, (1)h nmr and (13)c nmr spectral investigation. analytical studies suggest that the iron(iii)-quinolone complexes assume a six-coordinated dimeric distorted octahedral geometry. all the compounds show a good antibacterial activit ... | 2008 | 18343909 |
| response surface methodology to optimize the nutritional parameters for enhanced production of jasmonic acid by lasiodiplodia theobromae. | to find out the cumulative effect of the nutritional parameters and to enhance the production of jasmonic acid (ja) in static fermentation by lasiodiplodia theobromae using response surface methodology (rsm). | 2008 | 18397253 |
| separation and quantitation of jasmonic acid using hptlc. | a rapid, simple, and stringent protocol for the detection and quantitation of jasmonic acid (ja) is designed using high-performance thin-layer chromatography. acidified culture filtrate of lasiodiplodia theobromae is extracted with an equal volume of ethyl acetate and spotted on silica gel 60 f(254) foil using linomat-5 spray-on applicator. standard ja is also spotted either internally or adjacent to the sample, and the foils are developed with isopropanol-ammonia-water [10:1:1 (v/v)] as the mob ... | 2008 | 18402723 |
| new potato micro-tuber-inducing cyclohexene compounds related to theobroxide from lasiodiplodia theobromae. | two new cyclohexene compounds related to theobroxide (3) were isolated from the mycelia of lasiodiplodia theobromae ocs71. the structures of these compounds were determined to be (4s,5s)-4,5-dihydroxy-2-methyl-cyclohex-2-enone (1) and (3as,4r,5s,7ar)-4,5-dihydroxy-7-methyl-3a,4,5,7a-tetrahydrobenzo[1,3]dioxol-2-one (2) by means of spectroscopic analyses and chemical correlation to 3. compound 2 was shown to take up the carbonate ion to form a carbonic acid ester non-enzymatically. the compounds ... | 2008 | 18685216 |
| fungal diversity on fallen leaves of ficus in northern thailand. | fallen leaves of ficus altissima, f. virens, f. benjamina, f. fistulosa and f. semicordata, were collected in chiang mai province in northern thailand and examined for fungi. eighty taxa were identified, comprising 56 anamorphic taxa, 23 ascomycetes and 1 basidiomycete. common fungal species occurring on five host species with high frequency of occurrence were beltraniella nilgirica, lasiodiplodia theobromae, ophioceras leptosporum, periconia byssoides and septonema harknessi. colletotrichum and ... | 2008 | 18837113 |
| influence of culture media and environmental factors on mycelial growth and sporulation of lasiodiplodia theobromae (pat.) griffon and maubl. | lasiodiplodia theobromae, a common tea (camellia sinensis) pathogen, usually does not sporulate or sporulates poorly in common media, which makes spore production difficult. in this study the effects of culture media, carbon source, nitrogen source, temperature, ph and light on mycelial growth and sporulation were evaluated. among several carbon sources tested, glucose and sucrose were found superior for growth. potassium nitrate supplemented media showed maximum growth amongst the tested inorga ... | 2008 | 18972700 |
| fungal keratitis in far north queensland, australia. | aboriginals and torres strait islanders form a significant proportion of the population in far north queensland and are at increased risk for many eye conditions. this study describes the pattern of fungal keratitis seen at cairns base hospital, far north queensland, australia. | 2008 | 19128375 |
| biosynthesis of resorcylic acid lactone lasiodiplodin in lasiodiplodia theobromae. | the biosynthesis of lasiodiplodin (1) and its (5s)-5-hydroxylated derivative (2) were investigated by the administration of (13)c-labeled acetates to lasiodiplodia theobromae. the labeling patterns of biosynthetically (13)c-labeled 1 and 2 were determined by (13)c-nmr and inadequate spectra, demonstrating the octaketide origins of 1 and 2. taking into account the biosynthetic study of resorcylic acid lactones, the involvement of highly reduced acyl intermediates in the biosynthesis of lasiodiplo ... | 2009 | 19420710 |
| isolation and structural elucidation of a new cyclohexenone compound from lasiodiplodia theobromae. | a new cyclohexenone compound was isolated as a mixture of enantiomers from a culture filtrate of lasiodiplodia theobromae. the relative structure was determined to be 4,5-dihydroxy-3-methyl-cyclohex-2-enone on the basis of ms, (1)h-nmr, and (13)c-nmr spectroscopic analyses, including 2d-nmr experiments. resolution of the enantiomers was conducted by a coupling reaction with (s)-mtpa-cl followed by hplc separation. | 2009 | 19661717 |
| biosynthesis of resorcylic acid lactone (5s)-5-hydroxylasiodiplodin in lasiodiplodia theobromae. | an administration study of (2)h-labeled precursors showed that the 9-hydroxydecanoyl unit, the acyl intermediate of lasiodiplodin (1), was also the intermediate of (5s)-5-hydroxylasiodiplodin (2) in lasiodiplodia theobromae. the incorporation of [o-methyl-(2)h(3)]-lasiodiplodin (6) into 2 indicated that hydroxylation at c-5 occurred after cyclization. | 2009 | 19897901 |
| antifungal activities of ethanolic extract from jatropha curcas seed cake. | phorbol ester extraction was carried out from jatropha curcas seed cake, a by-product from the bio-diesel fuel industry. four repeated extractions from 5 g j. curcas seed cake using 15 ml of 90% (v/v) ethanol and a shaking speed of 150 rev/min gave the highest yield of phosbol esters. the ethanolic extract of j. curcas seed cake showed antifungal activities against important phytofungal pathogens: fusarium oxysporum, pythium aphanidermatum, lasiodiplodia theobromae, curvularia lunata, fusarium s ... | 2010 | 20208435 |
| maxillary sinusitis caused by lasiodiplodia theobromae. | lasiodiplodia (monotypic) comprises a very small proportion of the fungal biota. it is a common plant pathogen in tropical and subtropical regions. clinical reports on its association with onychomycosis, corneal ulcer and phaeohyphomycosis are available. however, lasiodiplodia theobromae causing fungal sinusitis has not been reported. we present here a case of fungal sinusitis in a 30-year-old woman, who came to the ent opd (out patient department) with complaints of intermittent bleeding and na ... | 2010 | 20404468 |
| biosynthesis of jasmonic acid in a plant pathogenic fungus, lasiodiplodia theobromae. | jasmonic acid (ja) is a plant hormone that plays an important role in a wide variety of plant physiological processes. the plant pathogenic fungus, lasiodiplodia theobromae also produces ja; however, its biosynthesis in this fungus has yet to be explored. administration of [1-(13)c] and [2-(13)c] naoac into l. theobromae established that ja in this fungus originates from a fatty acid synthetic pathway. the methyl ester of 12-oxo-phytodienoic acid (opda) was detected in the culture extracts of l. ... | 2010 | 20952041 |
| botryosphaeriaceae associated with pterocarpus angolensis (kiaat) in south africa. | there have been several recent reports of pterocarpus angolensis (kiaat) trees dying in south africa, zambia and zimbabwe, where this tree is used in traditional medicine and is a valuable source of timber for woodcarving and furniture. a survey of material from diseased p. angolensis trees in south africa yielded isolates of the botryosphaeriaceae, an important fungal family known to cause a number of tree diseases. the aim of this study was to identify these botryosphaeriaceae and to determine ... | 2010 | 21186325 |
| colonization of cashew plants by lasiodiplodia theobromae: microscopical features. | lasiodiplodia theobromae is a phytopathogenic fungus causing gummosis, a threatening disease for cashew plants in brazil. in an attempt to investigate the ultrastructural features of the pathogen colonization and its response to immunofluorescence labeling, light, confocal and electron microscope studies were conducted on different severity scale patterns of diseased plants. lasiodiplodia-antisera was checked for cross reactivity against common cashew plants fungi. optical microscopy analysis re ... | 2010 | 21194959 |
| [isolation of highly-effective benzo[a]pyrene degrading strain and its degradation capacity]. | one new mycete, which could degrade high concentration (up to 100 mg/l) of benzo[a]pyrene (bap) in liquid, was isolated from contaminated soil of beijing coking plant by gradually increasing the concentration of bap in mineral salt medium (msm) in order to get new microorganism species for remediation of bap contamination. the strain was identified as lasiodiplodia theobromae, and its biodegradation ability in liquid was further investigated. the results showed that l. theobromae could utilize b ... | 2011 | 22165241 |
| A new antifungal coumarin from Clausena excavata. | A new ?-lactone coumarin, named as excavarin-A, showing antifungal activity was isolated from the leaves of Clausena excavata by bioassay guided fractionation method. The structure was elucidated by spectroscopic data analysis and identified as 7((2E)-4(4,5-dihydro-3-methylene-2-oxo-5-furanyl)-3-methylbut-2-enyloxy) coumarin. Minimum inhibitory concentration (MIC) was determined against fifteen fungal strains pathogenic against plants and human. The least MIC was recorded against the human patho ... | 2012 | 22088496 |
| linolenate 9r-dioxygenase and allene oxide synthase activities of lasiodiplodia theobromae. | jasmonic acid (ja) is synthesized from linolenic acid (18:3n-3) by sequential action of 13-lipoxygenase, allene oxide synthase (aos), and allene oxide cyclase. the fungus lasiodiplodia theobromae can produce large amounts of ja and was recently reported to form the ja precursor 12-oxophytodienoic acid. the objective of our study was to characterize the fatty acid dioxygenase activities of this fungus. two strains of l. theobromae with low ja secretion (~0.2 mg/l medium) oxygenated 18:3n-3 to 5,8 ... | 2012 | 22048860 |
| diversity of plant oil seed-associated fungi isolated from seven oil-bearing seeds and their potential for the production of lipolytic enzymes. | commercial oil-yielding seeds (castor, coconut, neem, peanut, pongamia, rubber and sesame) were collected from different places in the state of tamil nadu (india) from which 1279 endophytic fungi were isolated. the oil-bearing seeds exhibited rich fungal diversity. high shannon-index h' was observed with pongamia seeds (2.847) while a low index occurred for coconut kernel-associated mycoflora (1.018). maximum colonization frequency (%) was observed for lasiodiplodia theobromae (176). dominance i ... | 2011 | 22806781 |
| high gene flow and outcrossing within populations of two cryptic fungal pathogens on a native and non-native host in cameroon. | in this study, we determined the genetic diversity of 126 isolates representing both lasiodiplodia theobromae and lasiodiplodia pseudotheobromae, collected from theobroma cacao and terminalia spp. in cameroon, using simple sequence repeat (ssr) markers. ssr alleles showed clear genetic distinction between l. theobromae and l. pseudotheobromae, supporting their earlier separation as sister species. both l. theobromae and l. pseudotheobromae populations from cameroon had high levels of gene divers ... | 2011 | 22385617 |
| sulfonation and anticoagulant activity of fungal exocellular β-(1→6)-d-glucan (lasiodiplodan). | an exocellular β-(1→6)-d-glucan (lasiodiplodan) produced by a strain of lasiodiplodia theobromae (mmlr) grown on sucrose was derivatized by sulfonation to promote anticoagulant activity. the structural features of the sulfonated β-(1→6)-d-glucan were investigated by uv-vis, ft-ir and (13)c nmr spectroscopy, and the anticoagulant activity was investigated by the classical coagulation assays aptt, pt and tt using heparin as standard. the content of sulfur and degree of substitution of the sulfonat ... | 2012 | 23399236 |