| [transformation of astragalus melilotoides pall with atnhx1 gene and the expression of salinity tolerance of transformants]. | using rt-pcr method, the open reading frame (orf) of atnhx1-cdna, encoding the vacuolar na+/h+ antiportor, was cloned from arabidopsis thaliana seedlings pretreated with 100 mmol/l nacl for 24h. this orf was inserted between camv35s promoter, a omega fragment of tmv rna 5'utr and nos polya terminator in the t-dna region of a binary expression vector pnt (fig1). the recombinant plasmid, designated as pnt-atnhx1, was then transformed into agrobacterium tumefaciens lba4404. mediated by this enginee ... | 2008 | 18630600 |
| [genetic diversity of rhizobia isolated from shrubby and herbaceous legumes in shenmu arid area, shaanxi, china]. | legume, with a strong resistance to the adverse environmental conditions, is one of pioneer plants in the desert region and plays an important role in the protection of the ecological environment. in this study, the symbiosis of rhizobia associating with shrubby and herbaceous legumes in shenmu area, shaanxi, china was characterized by the 16s rrna pcr-rflp and sequence analysis of involved genes. a total of 55 strains were isolated and purified, including 30 strains from the shrubby legume amor ... | 2014 | 25223023 |
| [protoplast culture and plant regeneration of the methionine resistant variant of astragalus melilotoides pall]. | an efficient protocol for plant regeneration from protoplasts of the methionine resistant variant of astragalus melilotoides was established. the friable calli induced from internode segments of variant plants were used for protoplast preparation. the protoplasts were isolated through enzyme digestion. calli were formed after sustained divisions of protoplasts. high frequency of shoot differentiation was obtained from the protocalli on differentiated medium. the effects of different media, cultu ... | 2004 | 15969112 |
| plant regeneration from protoplasts isolated from embryogenic calli of the forage legume astragalus melilotoides pall. | an efficient and reproducible protocol is described for the regeneration of astragalus melilotoides protoplasts isolated from hypocotyl-derived embryogenic calli. maximum protoplast yield (11.74 +/- 0.6x10(5)/g fw) and viability (87.07 +/- 2.8%) were achieved using a mixture of 2% (w/v) cellulase onozuka r10, 0.5% (w/v) cellulase onozuka rs, 0.5% (w/v) macerozyme r10, 0.5% (w/v) hemicellulase, and 1% (w/v) pectinase, all dissolved in a cell protoplast wash (cpw) salt solution with 13% (w/v) sorb ... | 2004 | 14762684 |
| [in vitro selection and characterization of methionine-resistant variant in astragalus melilotoides pall]. | a variant cell line of astragalus melilotoides pall. resistant to 14 mmol/l methionine was selected from calluses via mutagenesis with sodium azide. the plantlets were induced from the variant calluses. the regenerated variant plants were transplanted in soil and had set seeds. after the variant calluses had been cultured for 25 days in the medium containing 15 mmol/l methionine, the relative growth rate was 10.2 folds of the control's. free amino acids of the aspartate family in the variant cel ... | 2003 | 12858509 |