| chapare virus, a newly discovered arenavirus isolated from a fatal hemorrhagic fever case in bolivia. | a small focus of hemorrhagic fever (hf) cases occurred near cochabamba, bolivia, in december 2003 and january 2004. specimens were available from only one fatal case, which had a clinical course that included fever, headache, arthralgia, myalgia, and vomiting with subsequent deterioration and multiple hemorrhagic signs. a non-cytopathic virus was isolated from two of the patient serum samples, and identified as an arenavirus by ifa staining with a rabbit polyvalent antiserum raised against south ... | 2008 | 18421377 |
| genetic diversity among bolivian arenaviruses. | machupo virus and chapare virus are members of the tacaribe serocomplex (virus family arenaviridae) and etiological agents of hemorrhagic fever in humans in bolivia. the nucleotide sequences of the complete z genes, a large fragment of the rna-dependent rna polymerase genes, the complete glycoprotein precursor genes, and the complete nucleocapsid protein genes of 8 strains of machupo virus were determined to increase our knowledge of the genetic diversity among the bolivian arenaviruses. the res ... | 2009 | 19041349 |
| characterization of monoclonal antibodies to junin virus nucleocapsid protein and application to the diagnosis of hemorrhagic fever caused by south american arenaviruses. | junin virus (junv), machupo virus, guanarito virus, sabia virus, and chapare virus are members of new world arenavirus clade b and are the etiological agents of viral hemorrhagic fevers that occur in south america. in this study, we produced three monoclonal antibodies (mabs) to the recombinant nucleocapsid protein of junv, designated c6-9, c11-12, and e4-2. the specificity of these mabs was examined by enzyme-linked immunosorbent assay (elisa), indirect immunofluorescence assay, and an epitope- ... | 2009 | 19553554 |
| analysis of lujo virus cell entry using pseudotype vesicular stomatitis virus. | several arenaviruses are known to cause viral hemorrhagic fever (vhf) in sub-saharan africa and south america, where vhf is a major public health and medical concern. the biosafety level 4 categorization of these arenaviruses restricts their use and has impeded biological studies, including therapeutic drug and/or vaccine development. due to difficulties associated with handling live viruses, pseudotype viruses, which transiently bear arenavirus envelope proteins based on vesicular stomatitis vi ... | 2014 | 24741091 |
| possession, use, and transfer of select agents and toxins; biennial review. final rule. | in accordance with the public health security and bioterrorism preparedness and response act of 2002, the centers for disease control and prevention (cdc) located within the department of health and human services (hhs) has reviewed the list of biological agents and toxins that have the potential to pose a severe threat to public health and safety and is republishing that list. as a result of our review, we have added chapare virus, lujo virus, and sars-associated coronavirus (sars-cov) to the l ... | 2012 | 23038847 |
| an antibody recognizing the apical domain of human transferrin receptor 1 efficiently inhibits the entry of all new world hemorrhagic fever arenaviruses. | five new world (nw) arenaviruses cause human hemorrhagic fevers. four of these arenaviruses are known to enter cells by binding human transferrin receptor 1 (htfr1). here we show that the fifth arenavirus, chapare virus, similarly uses htfr1. we also identify an anti-htfr1 antibody, ch128.1, which efficiently inhibits entry mediated by the glycoproteins of all five viruses, as well as replication of infectious junín virus. our data indicate that all nw hemorrhagic fever arenaviruses utilize a co ... | 2012 | 22278244 |
| [arenavirus infections]. | arenaviruses are the collective name for viruses, which belong to the family arenaviridae. they replicate in the cytoplasm of cells, and were named after the sandy (latin, arenosus) appearance of the ribosomes often seen in thin sections of virions under electron microscope. several arenaviruses, such as lassa virus in west africa, and junin, guanarito, sabia, machupo, and chapare viruses in south america, cause sever viral hemorrhagic fevers (vhf) in humans and represent a serious public health ... | 2012 | 24153233 |
| serological assays based on recombinant viral proteins for the diagnosis of arenavirus hemorrhagic fevers. | the family arenaviridae, genus arenavirus, consists of two phylogenetically independent groups: old world (ow) and new world (nw) complexes. the lassa and lujo viruses in the ow complex and the guanarito, junin, machupo, sabia, and chapare viruses in the nw complex cause viral hemorrhagic fever (vhf) in humans, leading to serious public health concerns. these viruses are also considered potential bioterrorism agents. therefore, it is of great importance to detect these pathogens rapidly and spec ... | 2012 | 23202455 |