| molecular characterization of a distinct begomovirus species from vernonia cinerea and its associated dna-beta using the bacteriophage phi 29 dna polymerase. | vernonia cinerea plants with yellow vein symptoms were collected around crop fields in madurai. a portion (550 bp) of the av1 gene amplified using degenerate primers from the total dna purified from diseased leaf sample was cloned and sequenced. specific primers derived from the above sequence were used to amplify 2,745 nucleotides with the typical genome organization of begomoviral dna a (embl accession no. am182232). sequence comparison with other begomoviruses revealed the greatest identity ( ... | 2010 | 20401528 |