production and characterization of a group of bioemulsifiers from the marine bacillus velezensis strain h3.marine microbes are a rich source of bioactive compounds, such as drugs, enzymes, and biosurfactants. to explore the bioactive compounds from our marine natural product library, an oil emulsification assay was applied to discover biosurfactants and bioemulsifiers. a spore-forming bacterial strain from sea mud was found to produce bioemulsifiers with good biosurfactant activity and a broad spectrum of antimicrobial properties. it was identified as bacillus velezensis h3 using genomic and phenotyp ...201020473663
biocontrol mechanism by root-associated bacillus amyloliquefaciens fzb42 - a review.bacillus amyloliquefaciens subsp. plantarum fzb42 is a gram-positive model bacterium for unraveling plant-microbe interactions in bacilli. in addition, fzb42 is used commercially as biofertilizer and biocontrol agent in agriculture. genome analysis of fzb42 revealed that nearly 10% of the fzb42 genome is devoted to synthesizing antimicrobial metabolites and their corresponding immunity genes. however, recent investigations in planta demonstrated that - except surfactin - the amount of such compo ...201526284057
isolation and characterization of putative endophytic bacteria antagonistic to phoma tracheiphila and verticillium albo-atrum.a collection of 200 bacterial isolates recovered from citrus plants (citrus limon, citrus sinensis, and citrus reticulata), medicago truncatula and laurus nobilis, was established. in vitro screening indicated that 28 isolates exhibited an inhibitory activity against the vascular pathogens phoma tracheiphila and verticillium albo-atrum. isolates were screened according to their hydrolytic activities, plant growth-promoting bacteria (pgpb) abilities, as well as for the presence of nonribosomal pe ...201425074353
novel routes for improving biocontrol activity of bacillus based bioinoculants.biocontrol (bc) formulations prepared from plant-growth-promoting bacteria are increasingly applied in sustainable agriculture. especially inoculants prepared from endospore-forming bacillus strains have been proven as efficient and environmental-friendly alternative to chemical pesticides due to their long shelf life, which is comparable with that of agrochemicals. however, these formulations of the first generation are sometimes hampered in their action and do not fulfill in each case the expe ...201526696998
does the applicability of bacillus strains in probiotics rely upon their taxonomy?the taxonomic position and biological activities of two bacillus strains used in veterinary probiotics were studied in this work. these microorganisms inhibit growth of a broad spectrum of pathogenic cultures. they synthesize proteolytic enzymes and other biologically active metabolites, and to some extent, supplement each other with probiotic activities. it is not clear whether these versatile activities are properties of individual strains or bacterial taxa as whole. 16s rrna comparisons wer ...201222257263
relationship of bacillus amyloliquefaciens clades associated with strains dsm7t and fzb42: a proposal for bacillus amyloliquefaciens subsp. amyloliquefaciens subsp. nov. and bacillus amyloliquefaciens subsp. plantarum subsp. nov. based on their discriminating complete genome sequences.the whole genome sequenced rhizobacterium fzb42 (chen et al., 2007) and other plant-associated bacillus strains either designated as bacillus amyloliquefaciens or bacillus subtilis are used commercially to promote growth and health of crop plants. previous investigations revealed that the strains represent an own ecotype related to b. amyloliquefaciens, however its exact taxonomic position remains elusive (reva et al., 2004). here we have demonstrated ability to colonize arabidopsis roots for a ...201020817842
the characteristics of a novel heterotrophic nitrification-aerobic denitrification bacterium, bacillus methylotrophicus strain l7.bacillus methylotrophicus strain l7, exhibited efficient heterotrophic nitrification-aerobic denitrification ability, with maximum nh(4)(+)-n and no(2)(-)-n removal rate of 51.58mg/l/d and 5.81mg/l/d, respectively. strain l7 showed different gaseous emitting patterns from those strains ever described. when (15)nh(4)cl, or na(15)no(2), or k(15)no(3) was used, results of gc-ms indicated that n(2)o was emitted as the intermediate of heterotrophic nitrification or aerobic denitrification, while gc-i ...201222269053
genome analysis of bacillus amyloliquefaciens subsp. plantarum ucmb5113: a rhizobacterium that improves plant growth and stress management.the bacillus amyloliquefaciens subsp. plantarum strain ucmb5113 is a gram-positive rhizobacterium that can colonize plant roots and stimulate plant growth and defense based on unknown mechanisms. this reinforcement of plants may provide protection to various forms of biotic and abiotic stress. to determine the genetic traits involved in the mechanism of plant-bacteria association, the genome sequence of ucmb5113 was obtained by assembling paired-end illumina reads. the assembled chromosome of 3, ...201425119988
bacillus oryzicola sp. nov., an endophytic bacterium isolated from the roots of rice with antimicrobial, plant growth promoting, and systemic resistance inducing activities in rice.biological control of major rice diseases has been attempted in several rice-growing countries in asia during the last few decades and its application using antagonistic bacteria has proved to be somewhat successful for controlling various fungal diseases in field trials. two novel endophytic bacillus species, designated strains yc7007 and yc7010(t), with anti-microbial, plant growth-promoting, and systemic resistance-inducing activities were isolated from the roots of rice in paddy fields at ji ...201526060434
biological control of strawberry fusarium wilt caused by fusarium oxysporum f. sp. fragariae using bacillus velezensis bs87 and rk1 formulation.two isolates, bacillus sp. bs87 and rk1, selected from soil in strawberry fields in korea, showed high levels of antagonism towards fusarium oxysporum f. sp. fragariae in vitro. the isolates were identified as b. velezensis based on the homology of their gyra sequences to reference strains. bs87 and rk1 were evaluated for control of fusarium wilt in strawberries in pot trials and field trials conducted in nonsan, korea. in the pot trials, the optimum applied concentration of bs87 and rk1 for pre ...200919494701
a metaproteomic approach dissecting major bacterial functions in the rhizosphere of plants living in serpentine soil.a metaproteomic approach, based on liquid chromatography-high resolution mass spectrometry (lc-hrms) analysis, was followed to map the major bacterial metabolic functions associated with the rhizosphere of metal-tolerant and metal hyperaccumulator plants, growing in a serpentine soil naturally contaminated by heavy metals such as ni, co and cr. in particular, an "in-house" bacterial protein database was built based on the genera recognised by 16s rdna profiling, then it was used for protein iden ...201728083663
complete genome sequence of bacillus velezensis m75, a biocontrol agent against fungal plant pathogens, isolated from cotton waste.bacillus species have been widely used as biological control agents in agricultural fields due to their ability to suppress plant pathogens. bacillus velezensis m75 was isolated from cotton waste used for mushroom cultivation in korea, and was found to be antagonistic to fungal plant pathogens. here, we report the complete genome sequence of the m75 strain, which has a 4,007,450-bp single circular chromosome with 3921 genes and a g+c content of 46.60%. the genome contained operons encoding vario ...201727899334
gibberellins producing bacillus methylotrophicus ke2 supports plant growth and enhances nutritional metabolites and food values of lettuce.the nutritional quality of green leafy vegetables can be enhanced by application of plant beneficial micro-organisms. the present study was aimed to increase the food values of lettuce leaves by bacterial treatment. we isolated bacterial strain ke2 from kimchi food and identified as bacillus methylotrophicus by phylogenetic analysis. the beneficial effect of b. methylotrophicus ke2 on plants was confirmed by increasing the percentage of seed germination of lactuca sativa l., cucumis melo l., gly ...201627721133
complete genome sequence of the endophytic biocontrol strain bacillus velezensis cc09.bacillus velezensis is a heterotypic synonym of b. methylotrophicus, b. amyloliquefaciens subsp. plantarum, and bacillus oryzicola, and has been used to control plant fungal diseases. in order to fully understand the genetic basis of antimicrobial capacities, we did a complete genome sequencing of the endophytic b. velezensis strain cc09. genes tightly associated with biocontrol ability, including nonribosomal peptide synthetases, polyketide synthetases, iron acquisition, colonization, and volat ...201627688342
bacillus methylotrophicus sp. nov., a methanol-utilizing, plant-growth-promoting bacterium isolated from rice rhizosphere soil.a gram-positive bacterium, designated strain cbmb205(t), was isolated from the rhizosphere soil of traditionally cultivated, field-grown rice. cells were strictly aerobic, motile, rod-shaped and formed endospores. the best growth was achieved at 30°c and ph 7.0 in ammonium mineral salts (ams) medium containing 600 mm methanol. a comparative 16s rrna gene sequence-based phylogenetic analysis placed strain cbmb205(t) in a clade with the species bacillus amyloliquefaciens, bacillus vallismortis, ba ...201019966000
characterization and properties of biosurfactants produced by a newly isolated strain bacillus methylotrophicus dcs1 and their applications in enhancing solubility of hydrocarbon.six biosurfactant-producing bacteria were isolated from hydrocarbon contaminated soils in sfax, tunisia. isolates were screened for biosurfactant production by different conventional methods including hemolytic activity, surface tension reduction, drop-collapsing and oil displacement tests. all these screening tests show that all the isolates behave differently. among the isolated bacteria, dcs1 strain was selected for further studies based on its highest activities and it was identified as baci ...201627628335
construction of a recombinant bacillus velezensis strain as an integrated control agent against plant diseases and insect construct a new recombinant strain of bacillus velezensis that has antifungal and insecticidal activity via the expression of the insecticidal bacillus thuringiensis crystal protein, a b. thuringiensis expression vector (pht1k-1ac) was generated that contained the b. thuringiensis cry1ac gene under the control of its endogenous promoter in a minimal e. coli-b. thuringiensis shuttle vector (pht1k). this vector was introduced into a b. velezensis isolate that showed high antifungal activities a ...200919884784
complete genome sequence of bacillus velezensis cbmb205, a phosphate-solubilizing bacterium isolated from the rhizoplane of rice in the republic of korea.bacillus velezensis cbmb205 (= kacc 13105(t) = nccb 100236(t)) was isolated from the rhizoplane of rice (oryza sativa l. cv. o-dae). according to previous studies, this bacterium has several genes that can promote plant growth, such as the phosphorus-solubilizing protein-coding gene. here, we present the first complete genome of b. velezensis cbmb205.201627417835
in vitro evaluation of bioactive potential of bacillus methylotrophicus yml008 against propionibacterium acnes.acne vulgaris is the most common skin diseases that people experience during their lives. thirteen rhizosphere isolates were screened against propionibacterium acnes. the bacterium exhibited the highest activity against p. acnes was identified as bacillus methylotrophicus yml008 by 16s rrna gene sequencing. scanning electron microscopy was used to assess the changes in morphology of p. acnes. preliminary studies on the antimicrobial substance demonstrated the hydrophilic nature of compound with ...201627383487
azoreductase and dye detoxification activities of bacillus velezensis strain ab.azo dyes are known to be a very important and widely used class of toxic and carcinogenic compounds. although lot of research has been carried out for their removal from industrial effluents, very little attention is given to changes in their toxicity and mutagenicity during the treatment processes. present investigation describes isolation of a bacillus velezensis culture capable of degrading azo dye direct red 28 (dr28). azoreductase enzyme was isolated from it, and its molecular weight was fo ...200818034237
the complete genome sequence of bacillus velezensis 9912d reveals its biocontrol mechanism as a novel commercial biological fungicide agent.a bacillus sp. 9912 mutant, 9912d, was approved as a new biological fungicide agent by the ministry of agriculture of the people's republic of china in 2016 owing to its excellent inhibitory effect on various plant pathogens and being environment-friendly. here, we present the genome of 9912d with a circular chromosome having 4436 coding dna sequences (cdss), and a circular plasmid encoding 59 cdss. this strain was finally designated as bacillus velezensis based on phylogenomic analyses. genome ...201728235608
influence of bacillus amyloliquefaciens subsp. plantarum imv b-7404 strain exometabolites on phenylalanine ammonia-lyase activity in winter wheat seedlings.influence of bacillus amyloliquefaciens subsp. plantarum imv b-7404 strain exometabolites on phenylalanine ammonia-lyase (pal, ec activity in winter wheat seedlings has been studied. a significant increase of pal activity at 4-6 hours after treatment of plant roots with bacillus amyloliquefaciens subsp. plantarum imvb-7404 strain exometabolites and in case of leaves infection with bipolaris sorokiniana plant pathogen has been shown. it was established that pal activity changed along wi ...201627025068
bacillus velezensis is a later heterotypic synonym of bacillus amyloliquefaciens.strain bcrc 14193, isolated from soil, shared more than 99 % 16s rrna gene sequence similarity with bacillus amyloliquefaciens bcrc 11601(t) and bacillus velezensis bcrc 17467(t). this strain was previously identified as b. amyloliquefaciens, based on dna-dna hybridization, but its dna relatedness value with b. velezensis bcrc 17467(t) was 89 %. to investigate the relatedness of strain bcrc 14193, b. amyloliquefaciens and b. velezensis, the partial sequence of the gene encoding the subunit b pro ...200818319476
draft genome sequence of bacillus methylotrophicus fkm10, a plant growth-promoting rhizobacterium isolated from apple rhizosphere.bacillus methylotrophicus fkm10 is a strain of plant growth-promoting rhizobacterium with antimicrobial activity, which was isolated from apple rhizosphere. here, we present the genome sequence of b. methylotrophicus fkm10. two scaffolds were finally assembled, and several functional genes related to its antimicrobial activity were discovered.201626868409
biochemical and thermodynamic characterization of a novel, low molecular weight xylanase from bacillus methylotrophicus csb40 isolated from traditional korean food.a low molecular weight xylanase from bacillus strain csb40, isolated from traditional korean food and produced in beechwood xylan, was biochemically and thermodynamically characterized. it was purified 8.12-fold with a 15.88 % yield using deae sepharose fast flow, and it was determined to have a mass of ∼27 kda via sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and xylan zymography. the purified xylanase was optimally active at 50 °c and ph 6 and stable over a wide range of ...201626780766
β-1,3-1,4-glucanase gene from bacillus velezensis zj20 exerts antifungal effect on plant pathogenic fungi.bacillus velezensis is a known antifungal bacteria. to understand the role of β-1, 3-1, 4-glucanase played on b. velezensis about the mechanism which exerts effect on fungi, we isolated and cloned the β-1, 3-1, 4-glucanase gene (bglu1) from b. velezensis zj20. the bglu1 open reading frame was 732 bp that encoded a protein with 243 amino acids and a calculated molecular weight of 27.3 kda. the same gene without the signal peptide, termed bglu2, was also cloned and expressed in e. coli bl21. among ...201626745986
diversity and antimicrobial potential of bacterial isolates associated with the soft coral alcyonium digitatum from the baltic is well recognized that microorganisms associated with marine invertebrates, in particular sponges and hard corals, are an excellent source of new natural products. therefore, the diversity of bacteria associated with marine invertebrates and their potential to produce bioactive compounds have received much attention in recent years. we report here for the first time on the biodiversity of bacteria associated with the soft coral alcyonium digitatum, which is abundant in the baltic sea. in ord ...201626558794
bacillus velezensis sp. nov., a surfactant-producing bacterium isolated from the river vélez in málaga, southern spain.two gram-positive, endospore-forming bacterial strains, cr-502t and cr-14b, which produce surfactant molecules are described. phenotypic tests and phylogenetic analyses showed these strains to be members of the genus bacillus and related to the species bacillus atrophaeus, bacillus mojavensis, bacillus subtilis, bacillus vallismortis and bacillus amyloliquefaciens, although they differ from these species in a number of phenotypic characteristics. dna-dna hybridization confirmed that they show le ...200515653875
bacterial reduction in genotoxicity of direct red 28 red 28 (dr28) is a benzidine-based azo dye widely used in several countries. it has also been a subject of intense research for its anti-prion activity. like other benzidine-based azo dyes, it is also carcinogenic and toxic. however, there are very few studies addressing its detoxification. in the present study, a bacillus velezensis strain was used for detoxification of dr28. toxicity was checked by a battery of highly sensitive genotoxicity assays like comet assay, dna ladder formation, ...200919111330
rifampicin-resistance mutations in the rpob gene in bacillus velezensis cc09 have pleiotropic effects.rifampicin resistance (rif(r)) mutations in the rna polymerase β subunit (rpob) gene exhibit pleiotropic phenotypes as a result of their effects on the transcription machinery in prokaryotes. however, the differences in the effects of the mutations on the physiology and metabolism of the bacteria remain unknown. in this study, we isolated seven rif(r) mutations in rpob, including six single point mutations (h485y, h485c, h485d, h485r, q472r, and s490l) and one double point mutation (s490l/s617f) ...201728243227
production of bacillus amyloliquefaciens og and its metabolites in renewable media: valorisation for biodiesel production and p-xylene decontamination.biosurfactants are important in many areas; however, costs impede large-scale production. this work aimed to develop a global sustainable strategy for the production of biosurfactants by a novel strain of bacillus amyloliquefaciens. initially, bacillus sp. strain 0g was renamed b. amyloliquefaciens subsp. plantarum (syn. bacillus velezensis) after analysis of the gyra and gyrb dna sequences. growth in modified landy's medium produced 3 main recoverable metabolites: surfactin, fengycin, and aceto ...201727912317
genomic and metabolic traits endow bacillus velezensis cc09 with a potential biocontrol agent in control of wheat powdery mildew disease.bacillus velezensis cc09, which was isolated from healthy leaves of cinnamomum camphora and previously identified as bacillus amyloliquefaciens cc09, shows great potential as a new biocontrol agent, in control of many phytopathogenic diseases. to extend our understanding of the potential antifungal capacities, we did a whole genome analysis of strain cc09. result shows that strain cc09 has a relatively large genome size (4.17mb) with an average gc content of 46.1%, and 4021 predicted genes. thir ...201728164794
draft genome sequences of two lipopeptide-producing strains of bacillus methylotrophicus.bacillus methylotrophicus is implicated in phytostimulation and disease suppression of agricultural and bioenergy crops. here, we present the genome sequences of b. methylotrophicus strains b26 and ob9. their assembly resulted in 26 and 24 contigs, respectively. these strains are well suited for comparative genomics studies and the evaluation of commercially valuable biomolecular compounds.201526450741
plantazolicin is an ultra-narrow spectrum antibiotic that targets the bacillus anthracis membrane.plantazolicin (pzn) is a ribosomally synthesized and post-translationally modified natural product from bacillus methylotrophicus fzb42 and bacillus pumilus. extensive tailoring to twelve of the fourteen amino acid residues in the mature natural product endows pzn with not only a rigid, polyheterocyclic structure, but also antibacterial activity. here we report a remarkably discriminatory activity of pzn toward bacillus anthracis, which rivals a previously-described gamma (γ) phage lysis assay i ...201627152321
complete genome sequence of bacillus velezensis g341, a strain with a broad inhibitory spectrum against plant pathogens.bacillus velezensis g341 can suppress plant pathogens by producing antagonistic active compounds including bacillomycin d, fengycin, and (oxy) difficidin. the complete genome sequence of this bacterium was characterized by one circular chromosome of 4,009,746bp with 3953 open reading frames. the genome contained 36 pseudogenes, 30 rrna operons, and 95 trnas. this complete genome sequence provides an additional resource for the development of antimicrobial compounds.201526187870
bacillus amyloliquefaciens subsp. plantarum gr53, a potent biocontrol agent resists rhizoctonia disease on chinese cabbage through hormonal and antioxidants regulation.the fungus rhizoctonia solani is one of the causal agents of numerous diseases that affect crop growth and yield. the aim of this present investigation was to identify a biocontrol agent that acts against r. solani and to determine the agent's protective effect through phytohormones and antioxidant regulation in experimentally infected chinese cabbage plants. four rhizospheric soil bacterial isolates gr53, gr169, gr786, and gr320 were tested for their antagonistic activity against r. solani. amo ...201526160009
characterization and mechanism of anti-aeromonas salmonicida activity of a marine probiotic strain, bacillus velezensis v4.the bacterium aeromonas salmonicida is the causative agent of furunculosis, a systemic, ubiquitous disease of fish in the salmon family, characterized by high mortality and morbidity. probiotics are a promising approach for prevention of furunculosis in aquaculture. a bacterial strain with anti-a. salmonicida properties, bacillus velezensis v4, was isolated and the mechanisms underlying these properties were investigated. anti-a. salmonicida compounds present in cell-free supernatant of v4 were ...201728074223
[the taxonomic study of the strain bacillus sp. ucm b-7404--phytopathogenic fungae antagonist].the polyphasic taxonomic analysis of strain bacillus sp. ucm b-7404 active against phytopathogenic fungi and producing extracellular phytohormones, lytic enzymes and lipopeptide antifungal compounds has been carried out. the basic cell wall fatty acids presented by branched iso- and anteiso- c15:0 and c17:0 acids, contained 65-77% of the average pool. phylogenetic assay of 16s rrna gene nucleotide sequence, phenotypic and chemotaxonomic characteristics proved the attachment of strain bacillus sp ...201526036027
cyclic lipopeptides of bacillus amyloliquefaciens subsp. plantarum colonizing the lettuce rhizosphere enhance plant defense responses toward the bottom rot pathogen rhizoctonia solani.the commercially available inoculant bacillus amyloliquefaciens fzb42 is able to considerably reduce lettuce bottom rot caused by rhizoctonia solani. to understand the interaction between fzb42 and r. solani in the rhizosphere of lettuce, we used an axenic system with lettuce bacterized with fzb42 and inoculated with r. solani. confocal laser scanning microscopy showed that fzb42 could delay the initial establishment of r. solani on the plants. to show which secondary metabolites of fzb42 are pr ...201526011557
drna-seq reveals genomewide tsss and noncoding rnas of plant beneficial rhizobacterium bacillus amyloliquefaciens fzb42.bacillus amyloliquefaciens subsp. plantarum fzb42 is a representative of gram-positive plant-growth-promoting rhizobacteria (pgpr) that inhabit plant root environments. in order to better understand the molecular mechanisms of bacteria-plant symbiosis, we have systematically analyzed the primary transcriptome of strain fzb42 grown under rhizosphere-mimicking conditions using differential rna sequencing (drna-seq). our analysis revealed 4,877 transcription start sites for protein-coding genes, id ...201526540162
biological control of rice bakanae by an endophytic bacillus oryzicola our previous study, we reported that a novel endophytic bacterium bacillus oryzicola yc7007 has suppressed bacterial diseases of rice via induced systemic resistance and antibiotic production. this endophytic strain, b. oryzicola yc7007 was used as a biological control agent against bakanae disease of rice caused by fusarium fujikuroi, and its mechanism of interaction with the pathogen and the rice was further elucidated. root drenching with b. oryzicola yc7007 suspension reduced the disease ...201627298598
deciphering the conserved genetic loci implicated in plant disease control through comparative genomics of bacillus amyloliquefaciens subsp. understand the growth-promoting and disease-inhibiting activities of plant growth-promoting rhizobacteria (pgpr) strains, the genomes of 12 bacillus subtilis group strains with pgpr activity were sequenced and analyzed. these b. subtilis strains exhibited high genomic diversity, whereas the genomes of b. amyloliquefaciens strains (a member of the b. subtilis group) are highly conserved. a pairwise blastp matrix revealed that gene family similarity among bacillus genomes ranges from 32 to 90%, ...201526347755
identification of bacillus species occurring in kantong, an acid fermented seed condiment produced in ghana.kantong is a condiment produced in ghana by the spontaneous fermentation of kapok tree (ceiba pentandra) seeds with cassava flour as an additive. fermentation is over a 48h period followed by a drying and a kneading process. although lactic acid bacteria (lab) have previously been identified other micro-organisms may also be involved in the fermentation process. in this study we examined the occurrence of aerobic endospore-forming bacteria (aeb) in raw materials, during fermentation and in the f ...201424747716
purification and characterization of an intracellular levansucrase derived from bacillus methylotrophicus sk intracellular levansucrase from bacillus methylotrophicus sk 21.002 was isolated, purified, and characterized. the final specific levansucrase activity was 135.40 u/mg protein with an 11.78-fold enrichment and a 9.28% recovery rate. the molecular weight of the enzyme was approximately 60,000 da as evaluated by gel filtration and sds-page. both the maximum transfructosylation and hydrolytic activities were observed at ph 6.5. the enzyme exhibited optimum transfructosylation activity at 40 °c, ...201625524717
preliminary assessment on potentials of probiotic b. subtilis rx7 and b. methylotrophicus c14 strains as an immune modulator in salmonella-challenged weaned pigs.a total of 40 crossbred weaned piglets (28 days old; [landrace × yorkshire] × duroc) were used for preliminary assessment on potentials of bacillus-based probiotics as an immune modulator in a salmonella typhimurium challenge model in a 3-week experiment. pigs were randomly allotted to four experimental diets according to their initial body weight (9.21 ± 1.1 kg) and sex (10 pigs per treatment; 5 barrows and 5 gilts). the dietary treatments were basal diet (con), basal diet + oral administration ...201728326485
lipopeptides from a novel bacillus methylotrophicus 39b strain suppress agrobacterium crown gall tumours on tomato plants.this study aims to characterise the antibacterial activity of a novel bacillus methylotrophicus strain named 39b against tumourigenic agrobacterium tumefaciens c58 and b6 strains. it also aims to identify the compound that is responsible for its activity and to evaluate its efficiency to control crown gall disease in tomato plants.201727252109
scouting contaminated estuaries: heavy metal resistant and plant growth promoting rhizobacteria in the native metal rhizoaccumulator spartina maritima.spartina maritima is a native endangered heavy metal rhizoaccumulator cordgrass naturally growing in southwest coasts of spain, where is used as a biotool to rehabilitate degraded salt marshes. fifteen bacterial strains were isolated from the rhizosphere of s. maritima growing in the estuary of the tinto river, one of the most polluted areas in the world. a high proportion of bacteria were resistant towards several heavy metals. they also exhibited multiple plant growth promoting (pgp) propertie ...201525467875
complete genome sequence of a plant associated bacterium bacillus amyloliquefaciens subsp. plantarum ucmb5033.bacillus amyloliquefaciens subsp. plantarum ucmb5033 is of special interest for its ability to promote host plant growth through production of stimulating compounds and suppression of soil borne pathogens by synthesizing antibacterial and antifungal metabolites or priming plant defense as induced systemic resistance. the genome of b. amyloliquefaciens ucmb5033 comprises a 4,071,167 bp long circular chromosome that consists of 3,912 protein-coding genes, 86 trna genes and 10 rrna operons.201425197456
bacillus velezensis rc 218 as a biocontrol agent to reduce fusarium head blight and deoxynivalenol accumulation: genome sequencing and secondary metabolite cluster profiles.bacillus subtilis rc 218 was originally isolated from wheat anthers as a potential antagonist of fusarium graminearum, the causal agent of fusarium head blight (fhb). it was demonstrated to have antagonist activity against the plant pathogen under in vitro and greenhouse assays. the current study extends characterizing b. subtilis rc 218 with a field study and genome sequencing. the field study demonstrated that b. subtilis rc 218 could reduce disease severity and the associated mycotoxin (deoxy ...201627664721
inducible chromate reductase exhibiting extracellular activity in bacillus methylotrophicus for chromium bioremediation.chromium toxicity is one of the major causes of environmental pollution due to its heavy discharge in industrial wastewaters. chromate reduction is a viable method to detoxify hexavalent chromium to nontoxic trivalent species mediated by enzymes and metabolites. a new bacillus methylotrophicus strain was isolated from tannery sludge and was an efficient candidate for chromate reduction. an initial chromate reductase activity of 212.84 u/mg protein was obtained at 48 h in a low-cost defined mediu ...201524985094
biosynthesis of levan by levansucrase from bacillus methylotrophicus sk 21.002.a new strain sk 21.002 which could produce intracellular levansucrase was isolated from beet sugar growing soil. it was identified as bacillus methylotrophicus based on the sequence analysis of the 16s rrna and the morphological characteristic. the structure of levan biosynthesized from the levansucrase was determined to be connected through β-(2→6) linkages with fructofuranosyl residues. the molecular weight of the product was between 4 and 5 kda. the optimal conditions to produce levan using t ...201424299864
the rhizobacterium bacillus amyloliquefaciens subsp. plantarum nau-b3 contains a large inversion within the central portion of the genome.the genome of rhizobacterium bacillus amyloliquefaciens subsp. plantarum strain nau-b3 is 4,196,170 bp in size and harbors 4,001 genes. nine giant gene clusters are dedicated to the nonribosomal synthesis of antimicrobial lipopeptides and polyketides. remarkably, nau_b3 contains a large inversion within the central portion of the genome.201324233590
probiotics in salmonella-challenged hy-line brown layers.a total of 192 hy-line brown 40-week-old salmonella-free layers were randomly assigned to 4 dietary treatments in a 5-wk experiment to test the efficacy of probiotics on egg production and quality, excreta and intestinal microbiota of laying birds challenged with salmonella gallinarum. dietary treatment comprised of (1) nc: ; basal diet, negative control, (2) pc: ; basal diet + oral s. gallinarum administration, positive control, (3) t1: ; basal diet + 0.1% bacillus subtilis rx7 1 × 10(9) cfu/g ...201627053626
characterization and flocculation properties of a carbohydrate bioflocculant from a newly isolated bacillus velezensis this study, a bioflocculant with a high flocculation activity (> 98%) produced by strain 40b, which was isolated from a brackish water was investigated by 16s rdna sequence analysis, strain 40b was identified as bacillus velezensis. chemical analysis of the bioflocculant 40b indicated that it contained 2% protein and 98% carbohydrates. ftir analysis showed the presence of carboxyl, hydroxyl and amino groups, which were preferred for the flocculation process. the optimal concentration for the ...201324006807
simultaneous removal of organic matter and nitrogen by a heterotrophic nitrifying-aerobic denitrifying bacterial strain in a membrane bioreactor.a heterotrophic nitrifying-aerobic denitrifying bacterial strain, bacillus methylotrophicus l7, was inoculated solely into a submerged membrane bioreactor (mbr) for continuous treatment of artificial sewage. the running conditions were also optimized for improvement of the treatment efficiency. the results indicated that inoculation of this single strain in a single reactor under constant aerobic conditions resulted in simultaneous removal of organic matter and nitrogen, in striking contrast to ...201323792656
whole-genome sequencing of bacillus velezensis ls69, a strain with a broad inhibitory spectrum against pathogenic bacteria.bacillus velezensis ls69 was found to exhibit antagonistic activity against a diverse spectrum of pathogenic bacteria. it has one circular chromosome of 3,917,761bp with 3,643 open reading frames. genome analysis identified ten gene clusters involved in nonribosomal synthesis of polyketides (macrolactin, bacillaene and difficidin), lipopeptides (surfactin, fengycin, bacilysin and iturin a) and bacteriocins (amylolysin and amylocyclicin). in addition, b. velezensis ls69 was found to contain a ser ...201728323017
induction of systemic resistance against aphids by endophytic bacillus velezensis yc7010 via expressing phytoalexin deficient4 in arabidopsis.aphids are the most destructive insect pests. they suck the sap and transmit plant viruses, causing widespread yield loss of many crops. a multifunctional endophytic bacterial strain bacillus velezensis yc7010 has been found to induce systemic resistance against bacterial and fungal pathogens of rice. however, its activity against insects attack and underlying cellular and molecular defense mechanisms are not elucidated yet. here, we show that root drenching of arabidopsis seedlings with b. vele ...201728261260
key impact of an uncommon plasmid on bacillus amyloliquefaciens subsp. plantarum s499 developmental traits and lipopeptide production.the rhizobacterium bacillus amyloliquefaciens subsp. plantarum s499 (s499) is particularly efficient in terms of the production of cyclic lipopeptides, which are responsible for the high level of plant disease protection provided by this strain. sequencing of the s499 genome has highlighted genetic differences and similarities with the closely related rhizobacterium b. amyloliquefaciens subsp. plantarum fzb42 (fzb42). more specifically, a rare 8008 bp plasmid (ps499) harboring a rap-phr cassette ...201728154555
complete genome sequence of bacillus amyloliquefaciens subsp. plantarum s499, a rhizobacterium that triggers plant defences and inhibits fungal phytopathogens.bacillus amyloliquefaciens subsp. plantarum s499 is a plant beneficial rhizobacterium with a good antagonistic potential against phytopathogens through the release of active secondary metabolites. moreover, it can induce systemic resistance in plants by producing considerable amounts of surfactins. the complete genome sequence of b. amyloliquefaciens subsp. plantarum s499 includes a circular chromosome of 3,927,922bp and a plasmid of 8,008bp. a remarkable abundance in genomic regions of putative ...201627671697
the genome of plant growth-promoting bacillus amyloliquefaciens subsp. plantarum strain yau b9601-y2 contains a gene cluster for mersacidin synthesis.the genome of rhizobacterium bacillus amyloliquefaciens subsp. plantarum yau b9601-y2 was 4.24 mb in size and harbored 3,991 coding sequences (cds). giant gene clusters were dedicated to nonribosomal synthesis of antimicrobial lipopeptides and polyketides. remarkably, cau b946 possessed a gene cluster involved in synthesis of mersacidin.201222628498
analysis of plant growth-promoting properties of bacillus amyloliquefaciens ucmb5113 using arabidopsis thaliana as host plant.this study showed that bacillus amyloliquefaciens ucmb5113 colonizing arabidopsis roots changed root structure and promoted growth implying the usability of this strain as a novel tool to support sustainable crop production. root architecture plays a crucial role for plants to ensure uptake of water, minerals and nutrients and to provide anchorage in the soil. the root is a dynamic structure with plastic growth and branching depending on the continuous integration of internal and environmental f ...201727541497
scale-up of naringinase production process based on the constant oxygen transfer rate for a novel strain of bacillus methylotrophicus.naringinase bioprocess based on bacillus methylotrophicus was successfully scaled up based on constant oxygen transfer rate (otr) as the scale-up criterion from 5-l bioreactor to 20-l bioreactor. otr was measured in 5 and 20-l bioreactor under various operating conditions using dynamic method. the operating conditions, where complete dispersion was observed were identified. the highest otr of 0.035 and 0.04 mmol/l/s was observed in 5 and 20-l bioreactor, respectively. critical dissolved oxygen c ...201727340886
genome-wide identification and characterization of macrolide glycosyltransferases from a marine-derived bacillus strain and their phylogenetic distribution.clarifying glycosyltrasferases (gts) function is of significance for the development of gt inhibitors as drugs, and the use of gts to glycodiversify small molecules in the search of drug leads. while many actinomyces natural-product gts had been functionally characterized, our understanding towards bacillus natural-product gts is so far very limited. herein, genome-wide identification of macrolide gt genes from marine-derived bacillus methylotrophicus b-9987 revealed the presence of three macrol ...201627130432
isolation of hydroquinone (benzene-1,4-diol) metabolite from halotolerant bacillus methylotrophicus mhc10 and its inhibitory activity towards bacterial pathogens.a halotolerant bacterial isolate-mhc10 with broad spectrum antibacterial activity against clinical pathogens was isolated from saltpans located in tuticorin and chennai (india). 16s rrna gene analysis of mhc10 revealed close similarity to that of bacillus methylotrophicus. the culture conditions of b. methylotrophicus mhc10 strain were optimized for antibacterial production using different carbon and nitrogen sources, as well as varying temperature, ph, sodium chloride (nacl) concentrations and ...201626721574
complete genome sequence of bacillus methylotrophicus jj-d34 isolated from deonjang, a korean traditional fermented soybean paste.bacillus methylotrophicus jj-d34 showing good proteolytic and antipathogenic activities was isolated from doenjang, a korean traditional fermented soybean paste. here, we report the complete genome sequence of strain jj-d34 harboring a 4,105,955 bp circular chromosome encoding 4044 genes with a 46.24% g+c content, which will provide insights into the genomic basis of its effects and facilitating its application to doenjang fermentation.201626718560
bacillus velezensis is not a later heterotypic synonym of bacillus amyloliquefaciens; bacillus methylotrophicus, bacillus amyloliquefaciens subsp plantarum and 'bacillus oryzicola' are later heterotypic synonyms of bacillus velezensis based on phylogenomics.bacillus velezensis was previously reported to be a later heterotypic synonym of bacillus amyloliquefaciens, based on dna-dna relatedness values. we have sequenced a draft genome of b. velezensis nrrl b-41580t. comparative genomics and dna-dna relatedness calculations show that it is not a synonym of b. amyloliquefaciens. it was instead synonymous with bacillus methylotrophicus. 'bacillus oryzicola' is a recently described species that was isolated as an endophyte of rice (oryza sativa). the str ...201526702995
novel components of leaf bacterial communities of field-grown tomato plants and their potential for plant growth promotion and biocontrol of tomato diseases.this work aimed to characterize potentially endophytic culturable bacteria from leaves of cultivated tomato and analyze their potential for growth promotion and biocontrol of diseases caused by botrytis cinerea and pseudomonas syringae. bacteria were obtained from inner tissues of surface-disinfected tomato leaves of field-grown plants. analysis of 16s rrna gene sequences identified bacterial isolates related to exiguobacterium aurantiacum (isolates bt3 and mt8), exiguobacterium spp. (isolate gt ...201626654914
purification and characterization of a thermophilic 1,3-1,4-β-glucanase from bacillus methylotrophicus s2 isolated from extracellular 1,3-1,4-β-glucanase-producing strain s2 was isolated from booklice and identified as bacillus methylotrophicus. furthermore, a homogeneous extracellular 1,3-1,4-β-glucanase gcs2 was purified by ammonium sulfate precipitation and cation-exchange chromatography. the gene for the 1,3-1,4-β-glucanase was cloned, and the nucleotide sequence was determined. characterization of the purified enzyme revealed the molecular mass of 26 kda and the optimum activity at ph 7.5, 55°c. the purif ...201626589782
the lanthipeptides of bacillus methylotrophicus and their association with genomic islands.bacillus methylotrophicus strains are known for their potential as plant-growth promoters and as microbial pesticides that effectively control plant diseases caused by bacteria and fungi. over the past few years, a wide diversity of their secondary metabolites has been extensively characterized. among these are the ripps lanthipeptides, which are an important and growing group of notable compounds. the increasing interest in b. methylotrophicus species, accompanied by the development of high thr ...201526559891
enhancement of algicidal properties of immobilized bacillus methylotrophicus zju by coating with magnetic fe₃o₄ nanoparticles and wheat bran.algicidal bacteria offer a promising option for killing cyanobacteria. in this study, a newly isolated strain of bacillus methylotrophicus, zju, was used to control microcystis aeruginosa. analyses of relative reactive oxygen level, malondialdehyde content, superoxide dismutase activity, and fluorescence staining indicated that oxidative damage caused by the algicidal supernatant of strain zju mainly affected the cell membrane and consequently the membrane permeability and membrane potential of ...201626342577
bacillus methylotrophicus strain nkg-1, isolated from changbai mountain, china, has potential applications as a biofertilizer or biocontrol agent.chemical pesticides are widely used in agriculture, which endangers both environmental health and food safety. biocontrol is an environmentally-friendly and cost-effective green technique in environmental protection and agricultural production; it generally uses selected bioresources, including beneficial microorganisms. we isolated a novel bacterial strain (nkg-1) from the rare dormant volcanic soils of changbai mountain in china's jilin province. the strain was identified as bacillus methylotr ...201627832162
intracellular synthesis of glutamic acid in bacillus methylotrophicus sk19.001, a glutamate-independent poly(γ-glutamic acid)-producing strain.bacillus methylotrophicus sk19.001 is a glutamate-independent strain that produces poly(γ-glutamic acid) (γ-pga), a polymer of d- and l-glutamic acids that possesses applications in food, the environment, agriculture, etc. this study was undertaken to explore the synthetic pathway of intracellular l- and d-glutamic acid in sk19.001 by investigating the effects of tricarboxylic acid cycle intermediates and different amino acids as metabolic precursors on the production of γ-pga and analyzing the ...201626112100
phylogenomic analysis shows that bacillus amyloliquefaciens subsp. plantarum is a later heterotypic synonym of bacillus methylotrophicus.the rhizosphere-isolated bacteria belonging to the bacillus amyloliquefaciens subsp. plantarum and bacillus methylotrophicus clades are an important group of strains that are used as plant growth promoters and antagonists of plant pathogens. these properties have made these strains the focus of commercial interest. here, we present the draft genome sequence of b. methylotrophicus kacc 13105(t) ( = cbmb205(t)). comparative genomic analysis showed only minor differences between this strain and the ...201525835027
complete genome sequence of bacillus velezensis lm2303, a biocontrol strain isolated from the dung of wild yak inhabited qinghai-tibet plateau.bacillus velezensis lm2303 is a biocontrol strain with a broad inhibitory spectrum against plant pathogens, isolated from the dung of wild yak inhabited qinghai-tibet plateau, china. here we present its complete genome sequence, which consists of a single, circular chromosome of 3,989,393bp with a 46.68% g+c content. genome analysis revealed genes encoding specialized functions for the biosynthesis of antifungal metabolites and antibacterial metabolites, the promotion of plant growth, the allevi ...201728461206
bacillus methylotrophicus m4-96 isolated from maize (zea mays) rhizoplane increases growth and auxin content in arabidopsis thaliana via emission of volatiles.plant growth-promoting rhizobacteria stimulate plant growth and development via different mechanisms. in this study, we characterized the effect of volatiles from bacillus methylotrophicus m4-96 isolated from the maize rhizosphere on root and shoot development, and auxin homeostasis in arabidopsis thaliana. phytostimulation occurred after 4 days of interaction between m4-96 and arabidopsis grown on opposite sides of divided petri plates, as revealed by enhanced primary root growth, root branchin ...201728405774
green synthesis of silver nanoparticles by bacillus methylotrophicus, and their antimicrobial activity.the synthesis of silver nanoparticles (agnps) by microorganisms is an area attracting growing interest in nanobiotechnology, due to the applications of these nanoparticles in various products including cosmetics and biosensors, and in the biomedical, clinical, and bioimaging fields as well. various microorganisms have been found to be able to synthesize agnps when silver salts are supplied in the reaction system. the main objectives of this study were to evaluate the efficiency of synthesis of a ...201625749281
[antagonistic activity of bacillus amyloliquefaciens subsp. plantarum imv b-7404 and bim b-439d strains towards pathogenic bacteria and micromycetes].in this study the antagonistic activity of strains bacillus amyloliquefaciens subsp. plantarum imv b-7404 and bim b-439d against bacterial and fungal pathogens of agricultural crops has been researched. it is shown that both strains of bacilli demonstrated a high level of antagonism to the vascular bacteriosis pathogen, average level of antagonism to micromycetes--root rot pathogens. to ofiobulez pathogen strain b. amyloliquefaciens subsp. plantarum bim b-439d was more active. cultural liquid of ...201525639040
complete genome sequence of bacillus amyloliquefaciens subsp. plantarum cc178, a phyllosphere bacterium antagonistic to plant pathogenic fungi.bacillus amyloliquefaciens subsp. plantarum strain cc178 is a phyllosphere bacterium with antagonistic activity against a wide range of plant fungal pathogens. the genome of strain cc178 is 3,916,828 bp in size and harbors 3,972 genes. six giant gene clusters are dedicated to the nonribosomal synthesis of antimicrobial polypeptides and polyketides.201525573933
biosynthesis of lactosylfructoside by an intracellular levansucrase from bacillus methylotrophicus sk 21.002.lactosylfructoside is a functional oligosaccharide consisting of d-glucose, d-galactose, and d-fructose. in this work, lactosylfructoside was biosynthesized from sucrose as a fructosyl donor and lactose as an acceptor by an intracellular levansucrase derived from strain bacillus methylotrophicus (b. methylotrophicus) sk21.002. the trisaccharide was purified from the product using a high performance liquid chromatography (hplc) system and was confirmed to be lactosylfructoside by nuclear magnetic ...201525497128
in vitro assessment of the gastrointestinal tolerance and immunomodulatory function of bacillus methylotrophicus isolated from a traditional korean fermented soybean food.this study aimed to investigate the potential of bacillus methylotrophicus as a probiotic.201525494714
enhanced production of carboxymethylcellulase by a marine bacterium, bacillus velezensis a-68, by using rice hulls in pilot-scale bioreactor under optimized conditions for dissolved oxygen.the optimal conditions for the production of carboxymethylcellulase (cmcase) by bacillus velezensis a-68 at a flask scale have been previously reported. in this study, the parameters involved in dissolved oxygen in 7 and 100 l bioreactors were optimized for the pilot-scale production of cmcase. the optimal agitation speed and aeration rate for cell growth of b. velezensis a-68 were 323 rpm and 1.46 vvm in a 7 l bioreactor, whereas those for the production of cmcase were 380 rpm and 0.54 vvm, res ...201425079953
production of naringinase from a new soil isolate, bacillus methylotrophicus: isolation, optimization and scale-up studies.five strains of naringin-degrading bacteria were isolated and found to be positive for extracellular naringinase activity. the one that showed highest activity in the selective medium was identified by 16s rrna analysis as bacillus methylotrophicus . the best combination of carbon-nitrogen source was determined by employing two-level full factorial analyses, comprising 24 experiments in shake flasks. sucrose-yeast extract showed significant increase in naringinase activity (7.46 u/l) compared to ...201424152101
diversity and chemical defense role of culturable non-actinobacterial bacteria isolated from the south china sea gorgonians.the diversity of culturable non-actinobacterial (na) bacteria associated with four species of south china sea gorgonians was investigated using culture-dependent methods followed by analysis of the bacterial 16s rdna sequence. a total of 76 bacterial isolates were recovered and identified, which belonged to 21 species of 7 genera, and bacillus was the most diverse genus. fifty-one percent of the 76 isolates displayed antibacterial activities, and most of them belonged to the bacillus genus. from ...201323568196
genome sequence of the plant growth promoting strain bacillus amyloliquefaciens subsp. plantarum b9601-y2 and expression of mersacidin and other secondary metabolites.the plant-associated bacillus amyloliquefaciens subsp. plantarum strain b9601-y2, isolated from wheat rhizosphere, is a powerful plant growth-promoting rhizobacterium. its relative large genome size of 4.24mbp, exceeding that of other representatives of the b. amyloliquefaciens subsp. plantarum taxon, is mainly due to the presence of 18 dna-islands containing remnants of phages, a unique restriction modification system, a gene cluster for mersacidin synthesis, and an orphan gene cluster devoted ...201223357245
control of postharvest soft rot caused by erwinia carotovora of vegetables by a strain of bacillus amyloliquefaciens and its potential modes of action.erwinia carotovora subsp. carotovora (ecc), the causal agent of bacterial soft rot, is one of the destructive pathogens of postharvest vegetables. in this study, a bacterial isolate (bgp20) from the vegetable farm soil showed strong antagonistic activity against ecc in vitro, and its twofold cell-free culture filtrate showed excellent biocontrol effect in controlling the postharvest bacterial soft rot of potatoes at 25 °c. the anti-ecc metabolites produced by the isolate bgp20 had a high resista ...201323117674
phylogenetic diversity of bacterial endophytes of panax notoginseng with antagonistic characteristics towards pathogens of root-rot disease complex.endophytes play an important role in protection of host plants from infection by phytopathogens. endophytic bacteria were isolated from five different parts (root, stem, petiole, leaf and seed) of panax notoginseng and evaluated for antagonistic activity against fusarium oxysporum, ralstonia sp. and meloidogyne hapla, three major pathogens associated with root-rot disease complex of p. notoginseng. from 1000 endophytic bacterial strains evaluated in vitro, 104 strains exhibited antagonistic prop ...201322987248
the complete genome of bacillus amyloliquefaciens subsp. plantarum cau b946 contains a gene cluster for nonribosomal synthesis of iturin a.the genome of the rhizobacterium bacillus amyloliquefaciens subsp. plantarum cau b946 was 4.02 mb in size and harbored 3,823 genes (coding sequences [cds]). nine giant gene clusters were dedicated to nonribosomal synthesis of antimicrobial compounds. remarkably, strain cau b946 possessed a gene cluster involved in synthesis of iturin a.201222408246
antimicrobial peptide isolated from bacillus amyloliquefaciens k14 revitalizes its use in combinatorial drug therapy.the present study was performed to evaluate the antibacterial activities of an antimicrobial peptide (cspk14) and the synergies thereof with β-lactams against vancomycin-resistant staphylococcus aureus (vrsa) and enterococci (vre). our strain was isolated from fermented food (kimchi), which is 99.79 % homologous with bacillus amyloliquefaciens subsp. plantarum fzb42(t). cspk14 was purified to homogeneity by diammonium sulfate precipitation, concentration, dialysis, and followed by two-stage chro ...201727787755
complete genome sequence of bacillus methylotrophicus strain b25, a potential plant growth-promoting rhizobacterium.the complete genome of bacillus methylotrophicus strain b25, isolated in switzerland, was sequenced. its size is 3.85 mb, and several genes that may contribute to plant growth-promoting activities were identified in silico.201626966215
bacillus amyloliquefaciens, bacillus velezensis, and bacillus siamensis form an "operational group b. amyloliquefaciens" within the b. subtilis species complex.the plant growth promoting model bacterium fzb42(t) was proposed as the type strain of bacillus amyloliquefaciens subsp. plantarum (borriss et al., 2011), but has been recently recognized as being synonymous to bacillus velezensis due to phylogenomic analysis (dunlap c. et al., 2016). however, until now, majority of publications consider plant-associated close relatives of fzb42 still as "b. amyloliquefaciens." here, we reinvestigated the taxonomic status of fzb42 and related strains in its cont ...201728163698
glycin-rich antimicrobial peptide yd1 from b. amyloliquefaciens, induced morphological alteration in and showed affinity for plasmid dna of e. coli.antimicrobial peptides (amps), low-molecular-weight proteins with broad-spectrum antimicrobial activity, are the most promising candidates for the development of novel antimicrobials. a powerful cationic glycine-rich amp yd1 (mw ~ 1.0 kda) was purified from bacillus amyloliquefaciens cbsyd1 isolated from traditional korean fermented food kimchi, for the treatment of multidrug-resistant (mdr) bacteria. strain cbsyd1 was identified 99.79% similar to bacillus amyloliquefaciens subsp. plantarum fzb4 ...201728050849
complete genome sequence of bacillus velezensis gqjk49, a plant growth-promoting rhizobacterium with antifungal activity.bacillus velezensis gqjk49 is a plant growth-promoting rhizobacterium with antifungal activity, which was isolated from lycium barbarum l. rhizosphere. here, we report the complete genome sequence of b. velezensis gqjk49. twelve gene clusters related to its biosynthesis of secondary metabolites, including antifungal and antibacterial antibiotics, were predicted.201728860261
complete genome sequence of bacillus velezensis s3-1, a potential biological pesticide with plant pathogen inhibiting and plant promoting capabilities.antagonistic soil microorganisms, which are non-toxic, harmless non-pollutants, can effectively reduce the density of pathogenic species by some ways. bacillus velezensis strain s3-1 was isolated from the rhizosphere soil of cucumber, and was shown to inhibit plant pathogens, promote plant growth and efficiently colonize rhizosphere soils. the strain produced 13 kinds of lipopeptide antibiotics, belonging to the surfactin, iturin and fengycin families. here, we presented the complete genome sequ ...201728711664
the complete genome sequence of bacillus velezensis strain gh1-13 reveals agriculturally beneficial properties and a unique plasmid.the bacterial strain bacillus velezensis gh1-13, isolated from rice paddy soil in korea, has been shown to promote plant growth and have strong antagonistic activities against pathogens. here, we report the complete genome sequence of gh1-13, revealing that it possesses a single 4,071,980-bp circular chromosome with 46.2% gc-content. the chromosome encodes 3,930 genes, and we have also identified a unique plasmid in the strain that encodes a further 104 genes (71,628bp and 31.7% gc-content). the ...201728690133
structural characterization and identification of cyclic lipopeptides produced by bacillus methylotrophicus dcs1 strain.bacillus methylotrophicus dcs1 strain was isolated from diesel contaminated soil and screened for its ability to produce biosurfactants; it was found effective for the production of surface active molecules. the structural characterization of the isolated lipopeptides was studied by a variety of analytical techniques. the organic extract of dcs1 lipopeptides was fractionated by silica gel column chromatography (60mesh). fractions containing lipopeptides were collected and identified by tandem ma ...201728666229
complete genome sequence of biocontroller bacillus velezensis strain jtyp2, isolated from leaves of echeveria laui.bacillus velezensis jtyp2 was isolated from the leaves of echeveria laui in qingzhou, china, and may control some of the fungal pathogens of the plant. here, we present the complete genome sequence of b. velezensis jtyp2. several gene clusters related to its biosynthesis of antimicrobial compounds were predicted.201728619794
data on genome analysis of bacillus velezensis ls69.the data presented in this article are related to the published entitled "whole-genome sequencing of bacillus velezensis ls69, a strain with a broad inhibitory spectrum against pathogenic bacteria" (liu et al., 2017) [1]. genome analysis revealed b. velezensis ls69 has a good potential for biocontrol and plant growth promotion. this article provides an extended analysis of the genetic islands, core genes and amylolysin loci of b. velezensis ls69.201728560275
genome sequence of antibiotic-producing bacillus amyloliquefaciens strain kctc 13012.we report the 4.0-mb draft genome sequence of bacillus amyloliquefaciens (syn. bacillus velezensis) kctc 13012, which exhibits a broad spectrum of antagonistic activity against bacteria and fungi and promotes plant growth as well. the genome contains an array of biosynthetic gene clusters for secondary metabolites that are comparable to those in bacillus amyloliquefaciens subsp. plantarum fzb42(t).201526430040
small rna profiling reveals important roles for mirnas in arabidopsis response to bacillus velezensis fzb42.bacillus velezensis fzb42 (previously classified as bacillus amyloliquefaciens fzb42) has been confirmed to successfully colonize plant roots and enhance defense response against pathogen infection. this study indicated that fzb42 inoculation enhanced arabidopsis defense response against pseudomonas syringae dc3000 through inducing the expression of pr1, pdf1.2 and stomata closure. to further clarify the induced defense response at mirna level, srna libraries from arabidopsis roots inoculated wi ...201728754632
stimulation of fengycin-type antifungal lipopeptides in bacillus amyloliquefaciens in the presence of the maize fungal pathogen rhizomucor variabilis.most isolates belonging to the bacillus amyloliquefaciens subsp. plantarum clade retain the potential to produce a vast array of structurally diverse antimicrobial compounds that largely contribute to their efficacy as biocontrol agents against numerous plant fungal pathogens. in that context, the role of cyclic lipopeptides (clps) has been well-documented but still little is known about the impact of interactions with other soil-inhabiting microbes on the expression of these molecules. in this ...201728555132
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