| prevalence and identity of cryptosporidium spp. in pig slurry. | cryptosporidium spp. were detected in 25 of 56 pig slurry samples from 33 irish farms by pcr and dna sequencing. the organisms detected included c. suis, cryptosporidium pig genotype ii, and c. muris. we concluded that cryptosporidium oocysts can persist in treated slurry and potentially contaminate surface water through improper discharge or uncontrolled runoff. | 2006 | 16751569 |
| occurrence of cryptosporidium and giardia in suckling piglets in norway. | faecal samples from 684 litters of suckling piglets from 100 indoor swine herds from all regions of norway were examined for the presence of cryptosporidium and giardia, using sucrose gradient flotation concentration and immunofluorescent staining. thirty-one (31%) herds and 57 (8.3%) litters tested positive for cryptosporidium, while 10 (1.5%) litters in 10 different herds (10%) tested positive for giardia. molecular characterisation of nine cryptosporidium isolates demonstrated both c. suis an ... | 2007 | 17123737 |
| prevalence of cryptosporidium species in intensively farmed pigs in ireland. | natural cryptosporidium infections in pigs are widespread but generally apathogenic. this study was undertaken to determine the prevalence of zoonotic cryptosporidium spp. in piggeries in ireland, where the drinking water supply is particularly vulnerable to contamination with zoonotic species. overall, infections were detected in 39 out of 342 animals (11.4%), with highest infection rates among weaners (15%) and sows (13.3%). twenty-nine positive samples were genotyped based on ssu rrna sequenc ... | 2007 | 17565758 |
| molecular characterization of cryptosporidium isolates from pigs in zaragoza (northeastern spain). | a total of 142 stool specimens from pigs on 24 farms from the province of zaragoza (northeastern spain) were screened for cryptosporidium spp. samples were first analysed by routine techniques (formalin-ethyl acetate sedimentation method and modified ziehl-neelsen stain) selecting those microscopically positive for genetic characterization. cryptosporidium species and genotypes were determined by a nested pcr-rflp technique at the 18s ribosomal dna locus and sequencing of the pcr-positive second ... | 2007 | 17683866 |
| molecular characterization of cryptosporidium isolates from pigs at slaughterhouses in south bohemia, czech republic. | a total of 123 fecal samples of slaughtered finisher pigs and 21 sows from 14 farms were screened for cryptosporidium spp. infection using the aniline-carbol-methyl violet staining method. positive samples were molecularly characterized by direct sequencing of partial small subunit ribosomal rna (ssu rrna) and gp60 partial genes and polymerase chain reaction restriction fragment length polymorphism of ssu rrna. cryptosporidium oocysts were microscopically identified in 36 finishers (29%) and two ... | 2009 | 18850112 |
| prevalence and age-related infection of cryptosporidium suis, c. muris and cryptosporidium pig genotype ii in pigs on a farm complex in the czech republic. | a total of 413 pig faecal samples were collected from pre-weaners (119), starters (131), pre-growers (123) and sows (40) from a farm with a closed breeding system segmented into two breeding complexes and a growing complex in the region of vysocina, czech republic and screened for the presence of cryptosporidium using staining methods and genotyping (ssu rrna). cryptosporidium oocysts were detected by microscopy in the faeces of 21.1% of the samples (87/413). sequence analyses and rflp identifie ... | 2009 | 19091471 |
| cryptosporidium pig genotype ii in immunocompetent man. | | 2009 | 19523313 |
| concentrations, viability, and distribution of cryptosporidium genotypes in lagoons of swine facilities in the southern piedmont and in coastal plain watersheds of georgia. | waste lagoons of swine operations are a source of cryptosporidium oocysts. few studies, however, have reported on oocyst concentrations in swine waste lagoons; none have reported on oocyst viability status, nor has there been a systematic assessment of species/genotype distributions across different types of swine facilities. ten swine waste lagoons associated with farrowing, nursery, finishing, and gestation operations were each sampled once a month for a year. oocysts were extracted from tripl ... | 2010 | 20639363 |
| prevalence and molecular identification of cryptosporidium spp. in pigs in henan, china. | the distribution and public health significance of cryptosporidium species/genotypes in pigs differ among geographic areas and studies. to characterize the prevalence of cryptosporidiosis in pigs in henan, china, a total of 1,350 fecal samples from 14 farms in ten prefectures in henan province were examined. the overall prevalence of cryptosporidium was 8.2% (111/1,350), with the highest infection rate (79/383 or 20.6%) in 1-2-month-old piglets and the lowest infection rates in 3-6-month-old pig ... | 2010 | 20697908 |
| new view on the age-specificity of pig cryptosporidium by species-specific primers for distinguishing cryptosporidium suis and cryptosporidium pig genotype ii. | two species of cryptosporidium are commonly identified in pigs: cryptosporidium suis and cryptosporidium pig genotype ii. detection of cryptosporidium spp. is routinely based on molecular methods such as polymerase chain reaction (pcr) and subsequent restriction fragment length polymorphism (rflp) or gene sequencing. however, most of these methods are hampered by low sensitivity to mixed infections. as a solution of this problem, novel species-specific primers were designed and tested in the pre ... | 2010 | 21131129 |
| cryptosporidium pig genotype ii diagnosed in pigs from the state of rio de janeiro, brazil. | pigs may represent a source of cryptosporidium sp. infection to humans. the objective of this study was to identify the cryptosporidium species present in pigs from the state of rio de janeiro, brazil, and verify what risks pigs represent in the transmission of human cryptosporidiosis, because there is no such information to date in brazil. ninety-one samples of pig feces were collected from 10 piggeries in 2 municipalities located in the north and northwest regions of the state of rio de janeir ... | 2010 | 21348623 |
| prevalence of cryptosporidium spp. in pigs in shanghai, china. | a survey on prevalence of cryptosporidium spp. in pigs at 12 farms in 8 suburban districts and 1 county of shanghai was conducted under sheather's sucrose flotation protocol and modified acid-fast stain methods from 2006 to 2009. a total of 2323 faecal samples were collected and cryptosporidium spp. oocysts were detected in 800 samples (34.4%). cryptosporidium was found in all 12 pig farms. significant variations of prevalence were observed in different farms ranging from 14.1% to 90.6%. a follo ... | 2011 | 21616599 |
| detection and characterization of giardia duodenalis and cryptosporidium spp. on swine farms in ontario, canada. | abstract as part of the c-enternet surveillance program of the public health agency of canada, 122 pooled swine manure samples from 10 farms in ontario, canada were collected and tested for giardia and cryptosporidium. giardia duodenalis cysts and cryptosporidium spp. oocysts were detected using immunofluorescence microscopy. nested-polymerase chain reaction protocols were performed to amplify the small subunit rrna gene and the +¦-giardin gene for g. duodenalis, and the small subunit rrna gene ... | 2011 | 21675863 |
| prevalence of the cryptosporidium pig genotype ii in pigs from the yangtze river delta, china. | cryptosporidium spp. is prevalent globally, pigs are an important cryptosporidium reservoir. in china, little data regarding rates of cryptosporidium infections in pigs are available. the present study was therefore aimed at characterizing the distribution of cryptosporidium species in pigs from two different cities, shaoxing and shanghai, from the yangtze river delta. | 2011 | 21677776 |
| the first report on cryptosporidium suis and cryptosporidium pig genotype ii in eurasian wild boars (sus scrofa) (czech republic). | a total of 193 faecal samples of adult eurasian wild boars were collected at 12 enclosures across the czech republic and examined for cryptosporidium infection using both microscopic and molecular tools. cryptosporidium oocysts were not detected in any of the 193 faecal samples examined using the aniline-carbol-methyl violet staining method. thirty-two positive cases of cryptosporidium infection were detected using either genus- or species-specific nested pcr. mono-infection with cryptosporidium ... | 2011 | 21917378 |
| occurrence of giardia and cryptosporidium in pigs on prince edward island, canada. | in a cross-sectional study of 633 pigs from 21 herds on prince edward island, canada (pei), the prevalence of infection with cryptosporidium and giardia, and the genotypes and species of isolates were determined in order to establish the zoonotic potential of pigs in this region. as determined by direct immunofluorescence microscopy (dfa), 18 herds (86%) and 163 animals (26%; 95% ci: 22-29%) tested positive for cryptosporidium, while just 3 herds (14%) and 6 animals (1%; 95% ci: 0.4-2%) tested p ... | 2011 | 21868167 |