| taxon k, a complex within the burkholderia cepacia complex, comprises at least two novel species, burkholderia contaminans sp. nov. and burkholderia lata sp. nov. | the aim of the present study was to re-examine the taxonomic position and structure of taxon k (also known as group k) within the burkholderia cepacia complex (bcc). for this purpose, a representative set of strains was examined by a traditional polyphasic taxonomic approach, by multilocus sequence typing (mlst) analysis and by analysis of available whole-genome sequences. analysis of the reca gene sequence revealed three different lineages, designated reca-i, reca-ii and reca-iii. dna-dna hybri ... | 2009 | 19126732 |
| biosynthesis of an antifungal oligopeptide in burkholderia contaminans strain ms14. | strain ms14, exhibiting antifungal activity, was classified to belong to burkholderia contaminans. when compared with the wild type strain, antifungal activities of mutants ms14mt13 and ms14mt18 against geotrichum candidum were eliminated, and the activity of mutant ms14mt15 was reduced by approximately 80%. sequence analysis of a 22.7-kb dna segment revealed the presence of six open-reading frames (orfs), including the genes disrupted in the mutants. the wild type level of antifungal activity w ... | 2009 | 19167363 |
| rapid identification of burkholderia cepacia complex species including strains of the novel taxon k, recovered from cystic fibrosis patients by intact cell maldi-tof mass spectrometry. | two approaches based on intact cell matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (ic-maldi-tof ms) have been evaluated in order to discriminate and identify nine former burkholderia cepacia complex (bcc) species, burkholderia contaminans belonging to the novel taxon k, burkholderia gladioli, and the most relevant non-fermentative (nf) gram-negative rods recovered from cystic fibrosis (cf) sputum cultures. in total, 146 clinical isolates and 26 reference strains we ... | 2009 | 19475140 |
| ambr1 is a key transcriptional regulator for production of antifungal activity of burkholderia contaminans strain ms14. | burkholderia contaminans strain ms14 has a broad range of antifungal activities to plant and human pathogens. in previous studies, a 22.7-kb genomic fragment harboring six genes was shown to be involved in the production of an antifungal oligopeptide in b. contaminans strain ms14. in this study, another luxr-type regulatory gene, named ambr1, was identified downstream of the ambr2 gene, and three new orfs were found upstream of orf6 of the 22.7-kb fragment. site-directed mutagenesis revealed tha ... | 2009 | 19500142 |
| occidiofungin, a unique antifungal glycopeptide produced by a strain of burkholderia contaminans. | bacterial strain burkholderia contaminans ms14 was isolated from soil that suppressed brown patch disease of lawn grass. an antifungal compound was purified from the liquid culture of this bacterium. in this study, complete covalent structures of two purified closely related antifungal compounds were determined by the experiments of tocsy, noesy, roesy, 13c hsqc 2d nmr, and esi-ms and gc. the analysis of monoisotopic masses of the purified preparation indicated the presence of two related compou ... | 2009 | 19673482 |
| reca gene sequence and multilocus sequence typing for species-level resolution of burkholderia cepacia complex isolates. | to identify, by means of reca sequencing and multilocus sequence typing (mlst), burkholderia cepacia complex (bcc) isolates of environmental and clinical origin, which failed to be identified by reca rflp and species-specific pcr. | 2009 | 19732325 |
| hospital-wide outbreak of burkholderia contaminans caused by prefabricated moist washcloths. | we experienced a hospital outbreak of burkholderia contaminans (burkholderia cepacia group k) in a german university hospital with two campuses. cases were defined as the microbiological detection of b. cepacia complex (bcc) in any clinical specimen sent to the laboratory during 30 june to 21 october 2008. species identification of bcc was performed by reca gene sequencing, followed by pulsed-field gel electrophoresis (pfge; spei digest) for clonal identity. in total, 61 bcc-positive cases were ... | 2011 | 21216034 |
| genetic and biochemical map for the biosynthesis of occidiofungin, an antifungal produced by burkholderia contaminans strain ms14. | a striking feature of burkholderia contaminans strain ms14 is the production of a glycolipopeptide named occidiofungin. occidiofungin has a broad range of antifungal activities against plant and animal pathogens. in this study, a complete covalent structure characterization and identification of the whole genomic dna region for the occidiofungin gene (ocf) cluster are described. discovery of the presence of 2,4-diaminobutyric acid and 3-chloro-+¦-hydroxytyrosine and elucidation of the structure ... | 2011 | 21742901 |
| Occidiofungin's Chemical Stability and In vitro Potency Against Candida species. | Occidiofungin is a cyclic glyco-lipopeptide produced by Burkholderia contaminans. Minimum inhibitory concentrations (MICs) against Candida species were between 0.5 and 1.0 µg/mL. Occidiofungin retains its in vitro potency in the presence of 5% and 50% human serum with a minimal lethal concentration (MLC) of 2 and 4 µg/mL, respectively. Time-kill and post-antifungal effect (PAFE) experiments of occidiofungin against Candida albicans were measured. These results demonstrate that occidiofungin is f ... | 2011 | 22106210 |
| new pha products using unrelated carbon sources. | polyhydroxyalkanoates (pha) are natural polyesters stored by a wide range of bacteria as carbon source reserve. due to its chemical characteristics and biodegradability pha can be used in chemical, medical and pharmaceutical industry for many human purposes. over the past years, few burkholderia species have become known for production of pha. aside from that, these bacteria seem to be interesting for discovering new pha compositions which is important to different industrial applications. in th ... | 2011 | 24031764 |
| genetic diversity of burkholderia contaminans isolates from cystic fibrosis patients in argentina. | a total of 120 burkholderia cepacia complex isolates collected during 2004-2010 from 66 patients in two cystic fibrosis reference centers in argentina were analyzed. burkholderia contaminans was the species most frequently recovered (57.6%), followed by burkholderia cenocepacia (15%), a species distribution not reported so far. the reca-pcr-based techniques applied to the b. contaminans isolates revealed that 85% of the population carried the reca-st-71 allele. our results showed the utility of ... | 2013 | 23135937 |
| the tyrosine kinase bcef and the phosphotyrosine phosphatase bced of burkholderia contaminans are required for efficient invasion and epithelial disruption of a cystic fibrosis lung epithelial cell line. | bacterial tyrosine kinases and their cognate protein tyrosine phosphatases are best known for regulating the biosynthesis of polysaccharides. moreover, their roles in the stress response, dna metabolism, cell division, and virulence have also been documented. the aim of this study was to investigate the pathogenicity and potential mechanisms of virulence dependent on the tyrosine kinase bcef and phosphotyrosine phosphatase bced of the cystic fibrosis opportunistic pathogen burkholderia contamina ... | 2014 | 25486990 |
| draft genome sequences of burkholderia contaminans, a burkholderia cepacia complex species that is increasingly recovered from cystic fibrosis patients. | burkholderia contaminans belongs to the burkholderia cepacia complex (bcc), a group of bacteria that are ubiquitous in the environment and capable of infecting the immunocompromised and people with cystic fibrosis. we report here draft genome sequences for the b. contaminans type strain lmg 23361 and an argentinian cystic fibrosis sputum isolate. | 2015 | 26251482 |
| burkholderia contaminans biofilm regulating operon and its distribution in bacterial genomes. | biofilm formation by burkholderia spp. is a principal cause of lung chronic infections in cystic fibrosis patients. a "lacking biofilm production" (lbp) strain b. contaminans gimc4587:bct370-19 has been obtained by insertion modification of clinical strain with plasposon mutagenesis. it has an interrupted transcriptional response regulator (rr) gene. the focus of our investigation was a two-component signal transduction system determination, including this rr. b. contaminans clinical and lbp str ... | 2016 | 28070515 |
| burkholderia contaminans colonization from contaminated liquid docusate (colace) in a immunocompetent adult with legionnaire's disease: infection control implications and the potential role of candida pellucosa. | objective:b. contaminans was cultured from respiratory secretions and liquid docusate (colace) in a neurosurgical intensive care unit (nicu) patient with community-acquired legionnaire's disease but not from another bottle given to the patient. unexpectedly, c. pelliculosa was cultured from two bottles, but not the b. contaminans bottle or respiratory secretions. methods:b. cepacia, later identified as b. contaminans, was cultured from a bottle of liquid docusate (colace) dispensed to a non-cyst ... | 2016 | 27916878 |
| draft genome sequences of burkholderia contaminans ffi-28, a strain isolated from a contaminated pharmaceutical solution. | burkholderia contaminans is a species of the burkholderia cepacia complex, a group of bacteria that can grow in pharmaceutical products and are capable of infecting the immunocompromised and people with cystic fibrosis. here, we report draft genome sequences for burkholderia contaminans ffi-28, a strain isolated from a contaminated pharmaceutical solution. | 2016 | 27789640 |
| understanding the pathogenicity of burkholderia contaminans, an emerging pathogen in cystic fibrosis. | several bacterial species from the burkholderia cepacia complex (bcc) are feared opportunistic pathogens that lead to debilitating lung infections with a high risk of developing fatal septicemia in cystic fibrosis (cf) patients. however, the pathogenic potential of other bcc species is yet unknown. to elucidate clinical relevance of burkholderia contaminans, a species frequently isolated from cf respiratory samples in ibero-american countries, we aimed to identify its key virulence factors possi ... | 2016 | 27512997 |
| the first reported case of burkholderia contaminans in patients with cystic fibrosis in ireland: from the sargasso sea to irish children. | burkholderia contaminans is an emerging pathogen in the cystic fibrosis (cf) setting. included in the burkholderia cepacia complex (bcc), b. contaminans is a gram negative, motile, obligate aerobe previously classified as a pseudomonad. previous reports have described b. contaminans isolation from patients in portugal, switzerland, spain, argentina and the usa. this, however, is the first report relating to b. contaminans affecting irish patients with cf, initially detected in a paediatric setti ... | 2016 | 27103163 |
| snapbcebcon: a practical tool for identification and genotyping of burkholderia cepacia and burkholderia contaminans. | we propose an optimized protocol for an extensive population analysis of burkholderia cepacia and burkholderia contaminans. seven new polymorphisms were added to the recently proposed snapbcen assay, and a total of 18 markers ensured the clear identification and distinction of b. cepacia and b. contaminans isolates and high genotypic discrimination (simpson index of 0.94) compared to those for multilocus sequence typing. | 2016 | 26659211 |
| comparative genome-wide analysis reveals that burkholderia contaminans ms14 possesses multiple antimicrobial biosynthesis genes but not major genetic loci required for pathogenesis. | burkholderia contaminans ms14 shows significant antimicrobial activities against plant and animal pathogenic fungi and bacteria. the antifungal agent occidiofungin produced by ms14 has great potential for development of biopesticides and pharmaceutical drugs. however, the use of burkholderia species as biocontrol agent in agriculture is restricted due to the difficulties in distinguishing between plant growth-promoting bacteria and the pathogenic bacteria. the complete ms14 genome was sequenced ... | 2016 | 26769582 |
| the burkholderia contaminans ms14 ocfc gene encodes a xylosyltransferase for production of the antifungal occidiofungin. | burkholderia contaminans strain ms14 produces the antifungal compound occidiofungin, which is responsible for significant antifungal activities against a broad range of plant and animal fungal pathogens. occidiofungin is a cyclic glycolipopeptide made up of eight amino acids and one xylose. a 56-kb ocf gene cluster was determined to be essential for occidiofungin production. in this study, the ocfc gene, which is located downstream of ocfd and upstream of the ocfb gene in the ocf gene cluster, w ... | 2013 | 23435879 |
| polyclonal outbreak of bacteremia caused by burkholderia cepacia complex and the presumptive role of ultrasound gel. | a nosocomial polyclonal outbreak associated to bacteremia caused by different burkholderia cepacia complex (bcc) species and clones is reported. molecular characterization identified burkholderia stabilis, burkholderia contaminans, and burkholderia ambifaria among bcc isolates obtained from patients in neonatal and adult intensive care units. bcc was also isolated from an intrinsically contaminated ultrasound gel, which constituted the presumptive bcc source. prior bcc outbreak related to contam ... | 2015 | 26322722 |
| incidence of burkholderia contaminans at a cystic fibrosis centre with an unusually high representation of burkholderia cepacia during 15 years of epidemiological surveillance. | the burkholderia cepacia complex (bcc) is a heterogeneous group of bacteria comprising around 20 related species. these bacteria are important opportunistic pathogens, especially in cystic fibrosis (cf) patients, and are associated with a worse prognosis and decreased life expectancy. the taxonomic position of 20 bcc isolates retrieved from cf patients receiving care at hospital santa maria (hsm), in lisbon, from 1995 to 2006, was re-examined in the present work. these isolates, formerly classif ... | 2015 | 26002942 |
| increase in isolation of burkholderia contaminans from spanish patients with cystic fibrosis. | species of the burkholderia cepacia complex are associated with opportunistic infection in patients with cystic fibrosis. for years now, b. multivorans and b. cenocepacia have been the most frequently isolated species within the complex in such patients. however, between 2008 and 2012, the overall incidence of these species in spain (17.7% and 12.5% respectively) was overtaken by that of b. contaminans (36.5%). the population structure of b. contaminans isolates from spanish patients with cystic ... | 2015 | 25658563 |
| outbreak of bacteremia due to burkholderia contaminans linked to intravenous fentanyl from an institutional compounding pharmacy. | importance many health care facilities compound medications on site to fulfill local demands when customized formulations are needed, national supply is critically low, or costs for manufactured pharmaceuticals are excessive. small, institutional compounding facilities may perform the same high-risk procedures as large distributors of compounded medications. objectives to investigate an outbreak related to contamination of compounded sterile preparations and to determine processes to prevent fut ... | 2014 | 24493147 |
| clonally related burkholderia contaminans among ventilated patients without cystic fibrosis. | we investigated a cluster of 10 burkholderia cepacia complex-positive cultures among ventilated patients and those with a tracheostomy in an acute care hospital. isolates from 5 patients had outbreak-strain-related burkholderia contaminans. isolates of b. cepacia complex unrelated to the outbreak strain were cultured from a sink drain. the investigation identified practices that might have led to contamination of patient respiratory care supplies with tap water, which might have contributed to t ... | 2013 | 23973426 |
| performance of maldi-tof ms for species identification of burkholderia cepacia complex clinical isolates. | we evaluated the performance of matrix-assisted laser desorption ionization-time of flight (maldi-tof) for identification of bcc species compared with that of reca sequencing. maldi-tof was able of identifying 100% of bcc isolates at the genus level, but 23.1% of bcc isolates tested were not correctly identified at the species level. the misidentification occurred most frequently with burkholderia contaminans (100%) and b. cepacia (33.3%). | 2013 | 23891221 |
| burkholderia contaminans: unusual cause of biliary sepsis. | we report a case of biliary tract infection caused by a strain of burkholderia contaminans, a member of the burkholderia cepacia complex. the patient developed sepsis after endoscopic retrograde cholangiopancreatography (ercp). gram-negative bacilli were isolated from blood and bile cultures. automated bacterial identification systems identified the organism as burkholderia cepacia, whereas dna sequence analysis revealed that the reca gene isolate was identical to that of b. contaminans. the pat ... | 2013 | 23292160 |
| polyxydroxybutyrate and polyhydroxydodecanoate produced by burkholderia contaminans ipt553 phb and phdd produced by burkholderia. | in this paper, we introduce a new burkholderia contaminans capable of producing a newly characterized polymer methods and results: cg-ms and magnetic nuclear resonance (1) h and (13) c were used to determine the constitution of polymers produced on glucose, glucose with casein, sucrose, and sucrose with casein. three pairs of primers were used to find the pha synthase class and sequence. the synthesized polymers were composed by short chain length-pha (scl-pha), especially polyhydroxybutyrate (p ... | 2017 | 28383792 |
| the siderophore product ornibactin is required for the bactericidal activity of burkholderia contaminans ms14. | burkholderia contaminans ms14 was isolated from soil in mississippi. when it is cultivated on nutrient broth-yeast extract agar, the colonies exhibit bactericidal activity against a wide range of plant-pathogenic bacteria. a bacteriostatic compound with siderophore activity was successfully purified and was determined by nuclear magnetic resonance spectroscopy to be ornibactin. isolation of the bactericidal compound has not yet been achieved; therefore, the exact nature of the bactericidal compo ... | 2017 | 28188204 |
| bioconversion of ahx to aoh by resting cells of burkholderia contaminans ch-1. | fairy rings are zones of stimulated grass growth owing to the interaction between a fungus and a plant. we previously reported the discovery of two novel plant-growth regulating compounds related to forming fairy rings, 2-azahypoxanthine (ahx) and 2-aza-8-oxohypoxanthine (aoh). in this study, a bacterial strain ch-1 was isolated from an airborne-contaminated nutrient medium containing ahx. the strain converted ahx to aoh and identified as burkholderia contaminans based on the gene sequence of it ... | 2016 | 27387894 |
| antibacterial activity of silver camphorimine coordination polymers. | five new silver camphorimine complexes of general formula [ag(no3)(y)l] were synthesized and fully characterized using spectroscopic and analytical techniques. the structure of [ag(no3)(oc10h14nc6h4nc10h14o)] () was analyzed using single crystal x-ray diffraction, showing that it arranges as a coordination polymer formed by sequential ag(no3) units bridged by the bi-camphor ligand (). the antimicrobial properties of the new complexes were screened using the disk diffusion method and their minima ... | 2016 | 27007331 |
| occidiofungin is an important component responsible for the antifungal activity of burkholderia pyrrocinia strain lyc2. | to identify the taxonomy of tobacco rhizosphere-isolated strain lyc2 and investigate the mechanisms of the antifungal activities, focusing on antimicrobials gene clusters identification and function analysis. | 2016 | 26910858 |
| the antifungal occidiofungin triggers an apoptotic mechanism of cell death in yeast. | occidiofungin is a nonribosomally synthesized cyclic peptide having a base mass of 1200 da. it is naturally produced by the soil bacterium burkholderia contaminans ms14 and possesses potent broad-spectrum antifungal properties. the mechanism of action of occidiofungin is unknown. viability, terminal deoxynucleotidyl transferase dutp nick end labeling (tunel), reactive oxygen species (ros) detection, membrane and cell wall stability, and membrane mimetic assays were used to characterize the effec ... | 2013 | 23672235 |
| the presence of two cyclase thioesterases expands the conformational freedom of the cyclic peptide occidiofungin. | occidiofungin is a cyclic nonribosomally synthesized antifungal peptide with submicromolar activity produced by the gram-negative bacterium burkholderia contaminans. the biosynthetic gene cluster was confirmed to contain two cyclase thioesterases. nmr analysis revealed that the presence of both thioesterases is used to increase the conformational repertoire of the cyclic peptide. the loss of the ocfn cyclic thioesterase by mutagenesis results in a reduction of conformational variants and an appr ... | 2013 | 23394257 |
| nonclinical toxicological evaluation of occidiofungin, a unique glycolipopeptide antifungal. | occidiofungin, a glycolipopeptide obtained from the liquid culture of burkholderia contaminans ms14, has been identified as a novel fungicide. the present study was designed to initially assess the in vitro toxicity in a rat hepatoma (h4iie) cell line and acute toxicological effects of occidiofungin using a mouse model. in vitro toxicity was observed in all variables at 5 μmol/l. b6c3f1 mice were given single and repeat doses of occidiofungin up to 20 mg/kg. key effects were a reduction in body ... | 2012 | 22689636 |