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pittosporum cryptic virus 1: genome sequence completion using next-generation sequencing.next-generation sequencing (ngs) was applied to dsrnas extracted from an italian pittosporum plant infected with pittosporum cryptic virus 1 (picv1). ngs allowed assembly of the full genome sequence of picv1, comprising dsrna1 (1.9 kbp) and dsrna2 (1.5 kbp), which encode the rna-dependent rna polymerase and capsid protein genes, respectively. phylogenetic and sequence analyses confirmed that picv1 is a new member of the genus deltapartitivirus, family partiviridae. from the same plant, nsg also ...201627087112
sequence variation of block iii segment identifies three distinct lineages within eggplant mottled dwarf virus isolates from italy, spain and greece.partial polymerase (l) gene sequences of 919 nts, including the conserved segments pre-motif a and motif a of block iii, of 20 eggplant mottled dwarf virus (emdv) isolates were generated, and trimmed sequences of 889 nts, based on the length of available sequences of other isolates, were used to determine phylogenetic relationships. phylogenetic reconstructions revealed two divergent lineages, designated as genetic group a (italian isolates) and group b, with the latter further divided into subg ...201626982474
genetic variation of eggplant mottled dwarf virus from annual and perennial plant hosts.the genetic diversity of eggplant mottled dwarf virus (emdv), a member of the family rhabdoviridae, was studied using isolates collected from different herbaceous and woody plant species and remote geographic areas. sequences corresponding to the n, x, p, y, m and g orfs as well as the untranslated regions (utrs) between orfs were determined from all isolates. low genetic diversity was found in almost all genomic regions studied except for the x orf and the utrs, which were more variable, while ...201626660163
development of one-tube real-time qrt-pcr and evaluation of rna extraction methods for the detection of eggplant mottled dwarf virus in different species.a one-tube real-time qrt-pcr assay was developed, for the detection and quantification of eggplant mottled dwarf virus (emdv), a pathogen affecting cultivated and ornamental plants. the amplification efficiency of the assay was 98% and the linear range of quantification was from 20 to 2×10(8) rna transcripts. total rna extraction methods (three developed methods and one commercially available rna extraction kit) were evaluated using tissues from seven different plant species and synthetic emdv r ...201525445799
a novel strategy for the determination of a rhabdovirus genome and its application to sequencing of eggplant mottled dwarf virus.a novel strategy employing the rhabdovirus untranslated conserved intergenic regions was developed and applied successfully for the determination of the complete nucleotide sequence of eggplant mottled dwarf virus (emdv). the emdv genome contains seven open reading frames with the same organization as potato yellow dwarf virus (pydv), the type species of the genus nucleorhabdovirus. these two species encode five core genes [nucleocapsid (n), phosphoprotein (p), matrix (m), glycoprotein (g), and ...201323558438
lectins as probes for the assay of rhabdovirus infections in plants.thirteen different, biotinylated plant lectins were tested for their ability to recognize specifically the glycoproteins of the two different plant rhabdoviruses potato yellow dwarf virus and eggplant mottled dwarf virus. all viruses were propagated on the same plant host species, nicotiana rustica l. the lectin-binding to the viral proteins was tested after electrophoretic separation and transfer to nitrocellulose membranes. besides purified virus also partially pure virus preparations were use ...19873680462
a study of the structure of eggplant mottled dwarf virus. 19734139808
protein composition of the virions of five plant rhabdoviruses.five plant rhabdoviruses fall into two groups based on the patterns produced by their dissociated virion proteins when separated electrophoretically. lettuce necrotic yellows virus (lnvy) and sonchus virus (sv) virions contained only one m protein and ns protein. sowthistle yellow vein virus (syvv), sonchus yellow net virus (synv) and eggplant mottled dwarf virus (emdv) virions contained two m proteins and no detectable ns protein. the m and g proteins of lnyv and sv were easily released from th ...19817327907
highly specific detection of five exotic quarantine plant viruses using rt-pcr.to detect five plant viruses (beet black scorch virus, beet necrotic yellow vein virus, eggplant mottled dwarf virus, pelargonium zonate spot virus, and rice yellow mottle virus) for quarantine purposes, we designed 15 rt-pcr primer sets. primer design was based on the nucleotide sequence of the coat protein gene, which is highly conserved within species. all but one primer set successfully amplified the targets, and gradient pcrs indicated that the optimal temperature for the 14 useful primer s ...201325288934
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