Publications

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distribution of anopheles in vietnam, with particular attention to malaria vectors of the anopheles minimus complex.the distribution of anopheline mosquitoes in vietnam was examined, with a particular interest for the two sibling species of the anopheles minimus complex (cellia: myzomyia), an. minimus and anopheles harrisoni, respectively former species a and c. because the morphological identification of both sibling species is difficult and may lead to misidentifications, accurate data on their respective distribution are missing. this is of fundamental importance since the two species seem to exhibit diffe ...200818190697
the resting sites and blood-meal sources of anopheles minimus in taiwan.the who declared taiwan free from malaria in 1965, but in 2003 the reporting of two introduced cases in a rural area suggested a possible local transmission of this disease. therefore, understanding the resting sites and the blood sources of anopheles minimus is crucial in order to provide information for implementing vector control strategies.200818538036
genetic structure and gene flow of anopheles minimus and anopheles harrisoni in kanchanaburi province, thailand.isozyme frequencies were compared in seven field collections of anopheles minimus complex using starch gel electrophoresis. mosquito collections were sampled from four districts in kanchanaburi province where malaria is endemic. from eight enzyme systems, nine loci and seven polymorphisms were detected, indicating limited genetic differentiation among the seven collections (f(st) = 0.061). the highest percent polymorphic loci were observed in bong ti noi (btn) village (55.6%), whereas the least ...200818697319
behavioral responses of catnip (nepeta cataria) by two species of mosquitoes, aedes aegypti and anopheles harrisoni, in thailand.an investigation of the biological effect of catnip oil (nepeta cataria l.) on the behavioral response of field collected aedes aegypti and anopheles harrisoni was conducted using an automated excitorepellency test system. aedes aegypti showed significantly higher escape rates from the contact chamber at 5% catnip oil compared to other concentrations (p < 0.05). with anopheles harrisoni, a high escape response was seen at 2.5% catnip oil from the contact chamber, while in the noncontact chamber ...200819181058
vectors and malaria transmission in deforested, rural communities in north-central vietnam.malaria is still prevalent in rural communities of central vietnam even though, due to deforestation, the primary vector anopheles dirus is uncommon. in these situations little is known about the secondary vectors which are responsible for maintaining transmission. basic information on the identification of the species in these rural communities is required so that transmission parameters, such as ecology, behaviour and vectorial status can be assigned to the appropriate species.201020846447
the dominant anopheles vectors of human malaria in the asia-pacific region: occurrence data, distribution maps and bionomic precis.abstract: background: the final article in a series of three publications examining the global distribution of 41 dominant vector species (dvs) of malaria is presented here. the first publication examined the dvs from the americas, with the second covering those species present in africa, europe and the middle east. here we discuss the 19 dvs of the asian-pacific region. this region experiences a high diversity of vector species, many occurring sympatrically, which, combined with the occurrence ...201121612587
review of insecticide resistance and behavioral avoidance of vectors of human diseases in thailand.physiological resistance and behavioral responses of mosquito vectors to insecticides are critical aspects of the chemical-based disease control equation. the complex interaction between lethal, sub-lethal and excitation/repellent ('excito-repellent') properties of chemicals is typically overlooked in vector management and control programs. the development of "physiological" resistance, metabolic and/or target site modifications, to insecticides has been well documented in many insect groups and ...201324294938
evidence to support karyotypic variation of the mosquito, anopheles peditaeniatus in thailand.eight isoline colonies of anopheles peditaeniatus leicester (diptera: culicidae) were established from wild-caught females collected from buffalo-baited traps at 8 localities in thailand. they showed 2 types of x (x(2), x(3)) and 4 types of y (y(2), y(3), y(4), y(5)) chromosomes based on the number and amount of major block(s) of heterochromatin present in the heterochromatic arm, and were tentatively designated as forms b (x(2), x(3), y(2)), c (x(3), y(3)), d (x(3), y(4)) and e (x(2), x(3), y(5 ...021521137
mitochondrial dna variation in the malaria vector anopheles minimus across china, thailand and vietnam: evolutionary hypothesis, population structure and population history.the effects of pleistocene environmental fluctuations on the distribution and diversity of organisms in southeast asia are much less well known than in europe and north america. in these regions, the combination of palaeoenvironmental reconstruction and inferences about population history from genetic data has been very powerful. in southeast asia, mosquitoes are good candidates for the genetic approach, with the added benefit that understanding the relative contributions of historical and curre ...201020517346
absence of knockdown resistance suggests metabolic resistance in the main malaria vectors of the mekong region.as insecticide resistance may jeopardize the successful malaria control programmes in the mekong region, a large investigation was previously conducted in the mekong countries to assess the susceptibility of the main malaria vectors against ddt and pyrethroid insecticides. it showed that the main vector, anopheles epiroticus, was highly pyrethroid-resistant in the mekong delta, whereas anopheles minimus sensu lato was pyrethroid-resistant in northern vietnam. anopheles dirus sensu stricto showed ...200919400943
key knowledge gaps for plasmodium vivax control and elimination.there is inadequate understanding of the biology, pathology, transmission, and control of plasmodium vivax, the geographically most widespread cause of human malaria. during the last decades, study of this species was neglected, in part due to the erroneous belief that it is intrinsically benign. in addition, many technical challenges in culturing the parasite also hampered understanding its fundamental biology and molecular and cellular responses to chemotherapeutics. research on vivax malaria ...201627430544
host feeding patterns and preference of anopheles minimus (diptera: culicidae) in a malaria endemic area of western thailand: baseline site description.host feeding patterns of anopheles minimus in relation to ambient environmental conditions were observed during a 2-year period at tum sua village, located in mae sot district, tak province, in western thailand, where an. minimus is found in abundance and regarded as the most predominant malaria vector species. detailed information on mosquito behavior is important for understanding the epidemiology of disease transmission and developing more effective and efficient vector control methods.201222676415
malaria ecology along the thailand-myanmar border.malaria in southeast asia frequently clusters along international borders. for example, while most of thailand is malaria free, the border region shared with myanmar continues to have endemic malaria. this spatial pattern is the result of complex interactions between landscape, humans, mosquito vectors, and malaria parasites. an understanding of these complex ecological and socio-cultural interactions is important for designing and implementing malaria elimination efforts in the region. this art ...201526437860
the insecticide resistance status of malaria vectors in the mekong region.knowledge on insecticide resistance in target species is a basic requirement to guide insecticide use in malaria control programmes. malaria transmission in the mekong region is mainly concentrated in forested areas along the country borders, so that decisions on insecticide use should ideally be made at regional level. consequently, cross-country monitoring of insecticide resistance is indispensable to acquire comparable baseline data on insecticide resistance.200818534006
a single-round multiplex pcr assay for the identification of anopheles minimus related species infected with plasmodium falciparum and plasmodium vivax.this study aimed to develop a single-round multiplex pcr method for the identification of anopheles minimus complex (an. minimus and anopheles harrisoni) and anopheles aconitus subgroup (an. aconitus and anopheles varuna), and for the simultaneous detection of plasmodium falciparum and plasmodium vivax in these vectors. five primers were created for a single-round multiplex pcr assay to identify four anopheline mosquitoes combined with three plasmodium primers for the detection of p. falciparum ...201424239524
seasonal abundance and distribution of anopheles larvae in a riparian malaria endemic area of western thailand.three taxonomic groups of anopheles larvae were morphologically identified within the funestus group (minimus subgroup and aconitus subgroup) (75.63%), maculatus group (20.47%), and barbirostris group (0.57%) during a two-year period in conjunction with active malaria transmission in a village near the thai-myanmar border in kanchanaburi province, western thailand. the remaining 3.33% of anophelines collected were anopheles culicifacies (3.07%), anopheles philippinensis (0.17%), and anopheles va ...201223077839
behavioral responses of anopheles species (culicidae: diptera) with varying surface exposure to pyrethroid-treated netting in an excito-repellency test system.indoor residual spray (irs) with insecticides has been a procedure used for decades to protect humans from biting mosquitoes and potential vectors of pathogens. the objective of this study was to determine the responses of three wild-caught species of malaria vectors exposed to pyrethroids of three different surface coverage percents using an excito-repellency test box. each species was exposed to three insecticide-treated surfaces at varying exposure levels (full coverage, 50%, and 25% of the m ...201627860013
potential distribution of two species in the medically important anopheles minimus complex (diptera: culicidae).anopheles minimus theobald (=an. minimus a) and possibly anopheles harrisoni harbach & manguin (=an. minimus c) are important malaria vector species in the minimus complex in southeast asia. the distributions of these species are poorly known, although detailed information could benefit malaria vector incrimination and control. we used published collection records of these species and environmental geospatial data to construct consensus ecological niche models (enm) of each species' potential ge ...200818826026
an effective method for the identification and separation of anopheles minimus, the primary malaria vector in thailand, and its sister species anopheles harrisoni, with a comparison of their mating behaviors.species of the anopheles minimus complex are considered to be the primary vectors of malaria in south and southeast asia. two species of the complex, anopheles minimus and anopheles harrisoni, occur in thailand. they are sympatric and difficult to accurately distinguish based on morphological characters. the aim of this study was to investigate the potential of antennal sensory organs to distinguish these two species. additionally, we investigated their ability to mate in cages of different size ...201728222787
irritant and repellent responses of anopheles harrisoni and anopheles minimus upon exposure to bifenthrin or deltamethrin using an excito-repellency system and a live host.feeding responses of anopheles harrisoni and an. minimus were evaluated following exposure to 2 pyrethroid insecticides, bifenthrin or deltamethrin, using an excito-repellency test system in the presence and absence of live host cues. the results demonstrated that contact irritancy was the primary action of bifenthrin or deltamethrin in both mosquito species. there was no noncontact repellency effect elicited by either insecticide. anopheles minimus showed rapid escape response with high mortali ...201222533080
chemically induced behavioral responses in anopheles minimus and anopheles harrisoni in thailand.behavioral responses of female mosquitoes representing two species in the minimus complex exposed to an operational field dose of bifenthrin or deet (n,n-diethyl-m-toluamide) were described using an excito-repellency test system. two test populations of an. minimus, one from the field (tak province, western thailand), the other from a long-established laboratory colony, and anopheles harrisoni collected from kanchanaburi province, western thailand, were used. results showed that all test populat ...201122129403
locomotor behavioral responses of anopheles minimus and anopheles harrisoni to alpha-cypermethrin in thailand.excito-repellency responses of 3 test populations, representing 2 sibling species within the minimus complex, anopheles minimus and an. harrisoni, were characterized for contact irritant and noncontact repellent actions of chemicals during and after exposure to alpha-cypermethrin at half the recommended field (0.010 g/m2), the recommended field (0.020 g/m2), and double the recommended field concentration (0.040 g/m2), using an excito-repellency escape chamber system. two field populations of an. ...201122017085
discriminating lethal concentrations for transfluthrin, a volatile pyrethroid compound for mosquito control in thailand.pyrethroids are commonly used to control malaria and dengue vectors in thailand. the lack of specific lethal discriminating concentrations (dcs) for specific mosquito species has possibly compromised more accurate assessments of physiological susceptibility to various chemicals over time. previous studies have established dcs of various residual pyrethroids against specific mosquitoes in thailand. however, dcs for transfluthrin (tft), a highly volatile pyrethroid compound, against mosquito vecto ...201931922934
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