| alternative nitrogenase and pseudogenes: unique features of the paenibacillus riograndensis nitrogen fixation system. | biological nitrogen fixation (bnf) is a tightly regulated process that is carried out by diazotrophic microorganisms. the regulatory mechanisms of bnf-related genes are well characterized in gram-negative models, but they are poorly understood in gram-positive bacteria. paenibacillus riograndensis sbr5(t) is a gram-positive, endospore-forming facultative aerobic diazotroph. three clusters of bnf-related genes with dissimilar phylogenetic histories were identified in the p. riograndensis genome, ... | 2014 | 24956360 |
| complete genome sequence of paenibacillus riograndensis sbr5(t), a gram-positive diazotrophic rhizobacterium. | paenibacillus riograndensis is a gram-positive rhizobacterium which exhibits plant growth promoting activities. it was isolated from the rhizosphere of wheat grown in the state of rio grande do sul, brazil. here we announce the complete genome sequence of p. riograndensis strain sbr5(t). the genome of p. riograndensis sbr5(t) consists of a circular chromosome of 7,893,056bps. the genome was finished and fully annotated, containing 6705 protein coding genes, 87 trnas and 27 rrnas. the knowledge o ... | 2015 | 25959170 |
| iron deficiency resistance mechanisms enlightened by gene expression analysis in paenibacillus riograndensis sbr5. | despite its importance in growth and cell division, iron metabolism is still poorly understood in microorganisms, especially in gram-positive bacteria. in this work, we used rna sequencing technology to elucidate global mechanisms involved in iron starvation resistance in paenibacillus riograndensis sbr5, a potential plant growth-promoting bacterium. iron deficiency caused several changes in gene expression, and 150 differentially expressed genes were found: 71 genes were overexpressed and 79 ge ... | 2017 | 27130283 |
| how to transform a recalcitrant paenibacillus strain: from culture medium to restriction barrier. | paenibacillus riograndensis sbr5(t) is a plant growth-promoting bacterium isolated from the wheat rhizosphere. its recalcitrance to genetic manipulation is a major bottleneck for molecular studies, as has been reported for other paenibacillus environmental isolates. an efficient electroporation protocol was established by evaluating diverse parameters and optimizing the culture medium, culture growth phase, electroporation solution, recovery medium, dna input, and electric field strength. effici ... | 2016 | 27780731 |
| glutamine synthetase stabilizes the binding of glnr to nitrogen fixation gene operators. | biological nitrogen fixation (bnf) is a high energy demanding process carried out by diazotrophic microorganisms that supply combined nitrogen to the biosphere. the genes related to bnf are strictly regulated, but these mechanisms are poorly understood in gram-positive bacteria. the transcription factor glnr was proposed to regulate nitrogen fixation-related genes based on paenibacillus comparative genomics. in order to validate this proposal, we investigated bnf regulatory sequences in paenibac ... | 2017 | 28109177 |
| magnesium aminoclay-based transformation of paenibacillus riograndensis and paenibacillus polymyxa and development of tools for gene expression. | members of the genus paenibacillus are widespread facultative anaerobic, endospore-forming bacteria. some species such as paenibacillus riograndensis or paenibacillus polymyxa fix nitrogen and may play an important role in agriculture to reduce mineral nitrogen fertilization in particular for non-legume plants. the genetic manipulation of paenibacillus is an imperative for the functional characterization, e.g., of its plant growth-promoting activities and metabolism. this study showed that p. ri ... | 2017 | 27878581 |
| Genome sequence of the diazotrophic Gram-positive rhizobacterium Paenibacillus riograndensis SBR5(T). | Paenibacillus riograndensis SBR5(T), a nitrogen-fixing Gram-positive rhizobacterium isolated from a wheat field in the south of Brazil, has a great potential for agricultural applications due to its plant growth promotion effects. Here we present the draft genome sequence of P. riograndensis SBR5(T). Its 7.37-Mb genome encodes determinants of the diazotrophic lifestyle and plant growth promotion, such as nitrogen fixation, antibiotic resistance, nitrate utilization, and iron uptake. | 2011 | 22038959 |
| paenibacillus jilunlii sp. nov., a nitrogen-fixing species isolated from the rhizosphere of begonia semperflorens. | a nitrogen-fixing bacterium, designated strain be17(t), was isolated from rhizosphere soil of begonia semperflorens planted in beijing botanical garden, pr china. phylogenetic analyses based on a segment of the nifh gene sequence and a full-length 16s rrna gene sequence revealed that strain be17(t) was a member of the genus paenibacillus. high levels of 16s rrna gene sequence similarity were found between strain be17(t) and paenibacillus graminis rsa19(t) (97.9 %), paenibacillus sonchi lmg 24727 ... | 2010 | 20601486 |
| paenibacillus riograndensis sp. nov., a nitrogen-fixing species isolated from the rhizosphere of triticum aestivum. | a bacterial strain designated sbr5(t) was isolated from the rhizosphere of triticum aestivum. a phylogenetic analysis based on the 16s rrna gene sequence placed the isolate within the genus paenibacillus, being most closely related to paenibacillus graminis rsa19(t) (98.1 % similarity). the isolate was a gram-reaction-variable, motile, facultatively anaerobic bacterium, with spores in a terminal position in cells. starch was utilized and dihydroxyacetone and catalase were produced. strain sbr5(t ... | 2010 | 19648317 |