[studies on molluscicidal effect of bromoacetamide against ganesella virgo].molluscicidal effect of bromoacetamide against the snails in laboratory and field trials were observed. grassland of guiliu river in keerqinyouyiquanqi district, neimengol autonomous region was selected for field trials. the results showed that bromoacetamide was fairly effective against ganesella virgo, the intermediate host of dicrocoelium chinensis and eurytrema pancreaticum. in field trials, 7 days after spraying at a dosage of 0.5g/m2, 74.7-84.7% of snails were killed. when a dosage of 1g/m ...19902364507
characterization of dicrocoelium chinensis from domestic yaks in gansu and sichuan provinces, china, using genetic markers in two mitochondrial genes.dicrocoelium chinensis is commonly known as the "lancet fluke" or "small liver fluke" which lives in the bile ducts and gall bladder of domestic and wild ruminants. in this study, we examined sequence variability in two mitochondrial (mt) genes, namely cytochrome c oxidase subunit 1 (cox1) and nadh dehydrogenase subunit 1 (nad1) genes, among d. chinensis isolated from domestic yaks in gansu and sichuan provinces, china. a portion of the cox1 (pcox1) and nad1 (pnad1) genes was amplified by pcr se ...201323194108
dicrocoelium chinensis and dicrocoelium dendriticum (trematoda: digenea) are distinct lancet fluke species based on mitochondrial and nuclear ribosomal dna sequences.lancet flukes parasitize the bile ducts and gall bladder of a range of mammals, including humans, causing dicrocoeliosis. in the present study, we sequenced and characterized the complete mitochondrial (mt) genomes as well as the first and second internal transcribed spacers (its-1 and its-2=its) of nuclear ribosomal dna (rdna) of two lancet flukes, dicrocoelium chinensis and d. dendriticum. sequence comparison of a conserved mt gene and nuclear rdna sequences among multiple individual lancet fl ...201425016202
sequencing and characterization of the complete mitochondrial genome from the pancreatic fluke eurytrema pancreaticum (trematoda: dicrocoeliidae).the trematode eurytrema pancreaticum is a parasite of ruminant pancreatic and bile ducts, and also occasionally infects humans, causing eurytremiasis. in spite of it being a common fluke of cattle and sheep in endemic regions, little is known about the genomic resources of the parasite. we sequenced the complete mitochondrial (mt) genome of e. pancreaticum. it is 15,031 bp in size, and encodes 36 genes: 12 protein-coding genes, two ribosomal rna genes, and 22 transfer rna genes. the e. pancreati ...201626434796
detection of dicrocoelium dendriticum larval stages in mollusc and ant intermediate hosts by pcr, using mitochondrial and ribosomal internal transcribed spacer (its-2) sequences.summarythe aim of this study was to develop, perfect and validate the pcr (polymerase chain reaction) technique using mitochondrial (mt) and ribosomal (its-2) dna for the accurate identification of dicrocoelium dendriticum in molluscs and ants, the first and second intermediate hosts, and their early detection. the first primers that were designed amplified a 169 pb mtdna fragment of d. dendriticum permitted the detection of a single d. dendriticum metacercaria from the formica rufibarbis and fo ...201121861946
[the endoparasites of sika deer (cervus nippon) in austria].the endoparasite fauna of 108 sika deer (42 calves <1 year, 20 approximately 1 year old animals, 46 animals >1 year) originating from the two free-living sika deer populations in austria (ostrong, 35 animals; tullner donauauen, 73 animals) was studied. the deer were shot during the hunting seasons 2003-2005. in all, at least four species of protozoa (eimeria austriaca, eimeria robusta, eimeria sordida; sarcocystis spp.), two species each of cestodes (moniezia benedeni, larval cyst of taenia hyda ...200717987366
morphological and molecular differentiation between dicrocoelium dendriticum (rudolphi, 1819) and dicrocoelium chinensis (sudarikov and ryjikov, 1951) tang and tang, 1978 (platyhelminthes: digenea).dicrocoelium dendriticum (rudolphi, 1819) and dicrocoelium hospes (looss, 1907) are recognised to affect the liver of domestic and wild ruminants. a third species, dicrocoelium orientalis which was described from musk deer in the baikal region of the former soviet union and re-named to dicrocoelium chinensis (sudarikov and ryjikov, 1951) tang and tang, 1978 was isolated from other species of deer in asian countries and from mouflon and roe deer in europe. scant information is available for d. ch ...200717803950
morphometry on lancet flukes found in japanese sika deer (cervus nippon centralis) captured in iwate prefecture, japan.thirty-six flukes were collected from the livers of wild deer (cervus nippon centralis) captured in iwate prefecture, japan, and were served for morphometry. the length and/or the width of the body, suckers, testes, ovary, vitelline glands, cirrus pouch and eggs in the uterus of the flukes were measured. the distance between anterior end of the body and position of the maximal body-width or upper end of the testes were also determined. a remarked morphological characteristic was that the right a ...200616679730
mitochondrial genome of ogmocotyle sikae and implications for phylogenetic studies of the notocotylidae trematodes.ogmocotyle spp. (trematoda: digenea: notocotylidae) are neglected but important trematodes that can infect numerous mammal species, causing significant economic losses to livestock industries. however, there have been few studies on the molecular ecology of these trematodes. we amplified and sequenced the complete mitochondrial (mt) genome of ogmocotyle sikae (14,307 bp). the gene content and gene arrangement of o. sikae mt genome was similar to that of dicrocoelium chinensis, except that trne a ...201626594020
phylogenetic relationships between dicrocoelium chinensis populations in japan and china based on mitochondrial nad1 gene sequences.we carried out phylogenetic analyses of the relationships between dicrocoelium chinensis populations in japan and china using molecular markers. one hundred nine lancet flukes collected from japan and china were identified as d. chinensis based on their testis orientation and the nucleotide sequences of their ribosomal its2. these flukes were analyzed phylogenetically using mitochondrial nad1 gene sequences. an analysis of molecular variance found that the percentage of variation between the cou ...201728735469
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