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observations on isospora suis infection in a minimal disease pig herd.faeces samples from 11 sows and their litters and intestinal contents and scrapings collected from 56 piglets at the time of slaughter were examined for isospora suis. the findings indicate that oocysts with sporoblasts can be found in faeces samples 24 hours after collection; the prepatent period is about six to nine days and the patent period three to eight days (adjudged under non-experimental conditions); piglets in an infected herd are normally resistant to natural infection for the first t ...19761274145
observations on isospora suis infection in a minimal disease pig herd.faeces samples from 11 sows and their litters and intestinal contents and scrapings collected from 56 piglets at the time of slaughter were examined for isospora suis. the findings indicate that oocysts with sporoblasts can be found in faeces samples 24 hours after collection; the prepatent period is about six to nine days and the patent period three to eight days (adjudged under non-experimental conditions); piglets in an infected herd are normally resistant to natural infection for the first t ...19761274145
development and characterization of monoclonal antibodies to first-generation merozoites of eimeria bovis.merozoites of eimeria bovis were harvested from bovine monocyte cell cultures and used to immunize balb/c mice. spleens from immunized mice were removed and the cells fused with mouse myeloma cells. supernates from resulting hybridoma cell lines were examined for antibodies to first-generation e. bovis merozoites using an indirect immunofluorescent antibody (ifa) assay. three positive cell lines were identified and cloned by limiting dilution. all three cell lines produced immunoglobulins of the ...19921281590
development and characterization of monoclonal antibodies to first-generation merozoites of eimeria bovis.merozoites of eimeria bovis were harvested from bovine monocyte cell cultures and used to immunize balb/c mice. spleens from immunized mice were removed and the cells fused with mouse myeloma cells. supernates from resulting hybridoma cell lines were examined for antibodies to first-generation e. bovis merozoites using an indirect immunofluorescent antibody (ifa) assay. three positive cell lines were identified and cloned by limiting dilution. all three cell lines produced immunoglobulins of the ...19921281590
demonstration of isospora suis oocysts in faecal samples. 19921455595
demonstration of isospora suis oocysts in faecal samples. 19921455595
[beta-d-glucosidase in the microvillous zone of small intestine enterocytes in experimental coccidiosis in suckling piglets].in the small intestine mucosa of 24 gnotobiotic farrows experimentally infected with the oocysts of coccidiosis of isospora suis (infection administration--100,000 oocysts) on the first day after the delivery, we carried out the microdensitometric evaluation of the activity of beta-d-glucosidase (phlorizin-hydrolase; hetero-beta-galactosidase; lactase-beta-glucosidase complex; ec. 3.2.1.21). great attention was paid to the topochemistry of enzyme, deposited in a microvillous zone of enterocytes. ...19911771725
[beta-d-glucosidase in the microvillous zone of small intestine enterocytes in experimental coccidiosis in suckling piglets].in the small intestine mucosa of 24 gnotobiotic farrows experimentally infected with the oocysts of coccidiosis of isospora suis (infection administration--100,000 oocysts) on the first day after the delivery, we carried out the microdensitometric evaluation of the activity of beta-d-glucosidase (phlorizin-hydrolase; hetero-beta-galactosidase; lactase-beta-glucosidase complex; ec. 3.2.1.21). great attention was paid to the topochemistry of enzyme, deposited in a microvillous zone of enterocytes. ...19911771725
[histochemistry of acid phosphatase in small intestine mucosa in experimental coccidiosis in suckling piglets].the activity of acid phosphatase (phosphohydrolase of orthophosphate monoesters; ec. 3.1.3.2) was evaluated densitometrically in the mucosa of duodenum, jejunum and ileum of 22 conventional piglets which were experimentally infected by oocysts of the coccidiae isospora suis (infection dose of 200,000 oocysts) on day one after parturition (dap). the activity of the studied hydrolase was investigated in the infected piglets during days two to ten after infection (dai) in the intestinal mucosa (ent ...19911807026
[histochemistry of acid phosphatase in small intestine mucosa in experimental coccidiosis in suckling piglets].the activity of acid phosphatase (phosphohydrolase of orthophosphate monoesters; ec. 3.1.3.2) was evaluated densitometrically in the mucosa of duodenum, jejunum and ileum of 22 conventional piglets which were experimentally infected by oocysts of the coccidiae isospora suis (infection dose of 200,000 oocysts) on day one after parturition (dap). the activity of the studied hydrolase was investigated in the infected piglets during days two to ten after infection (dai) in the intestinal mucosa (ent ...19911807026
[use of the anticoccidial agent, tortrazuril (baycox, bayer) in coccidiosis in suckling pigs].at present neonatal coccidiosis caused by isospora suis is known to be an important disease in piglets. the coccidium i. suis was diagnosed as the causative agent of diarrhoeic disease in piglets on a large pig farm with continuous farrowing operation. the diagnosis of coccidiosis was based on clinical history, gross lesions, histopathology, stained impression smears and aided by detection of oocysts in the faeces. coccidiosis was associated with diarrhoea in piglets at the age of five to fourte ...19911841476
[use of the anticoccidial agent, tortrazuril (baycox, bayer) in coccidiosis in suckling pigs].at present neonatal coccidiosis caused by isospora suis is known to be an important disease in piglets. the coccidium i. suis was diagnosed as the causative agent of diarrhoeic disease in piglets on a large pig farm with continuous farrowing operation. the diagnosis of coccidiosis was based on clinical history, gross lesions, histopathology, stained impression smears and aided by detection of oocysts in the faeces. coccidiosis was associated with diarrhoea in piglets at the age of five to fourte ...19911841476
pathogenesis of experimental combined infections with isospora suis and rotavirus in conventional and gnotobiotic piglets.51 gnotobiotic and 63 conventional, one-, or two-days-old piglets were divided into five groups and infected orally either with isospora suis or rotavirus alone, or with both agents simultaneously or successively with alternative sequences and various intervals. 15 gnotobiotic and 10 conventional piglets served as controls. the development of small intestinal lesions after infection with i. suis was biphasic. the dominant alteration resulting from rotavirus infection was villus atrophy, consider ...19911858460
pathogenesis of experimental combined infections with isospora suis and rotavirus in conventional and gnotobiotic piglets.51 gnotobiotic and 63 conventional, one-, or two-days-old piglets were divided into five groups and infected orally either with isospora suis or rotavirus alone, or with both agents simultaneously or successively with alternative sequences and various intervals. 15 gnotobiotic and 10 conventional piglets served as controls. the development of small intestinal lesions after infection with i. suis was biphasic. the dominant alteration resulting from rotavirus infection was villus atrophy, consider ...19911858460
experimental isospora suis infections in miniature swine.clinical responses to experimental isospora suis infections were compared in sinclair miniature pigs and cross-bred conventional pigs. pre-patent periods, fecal consistencies, oocyst excretion dynamics, trends in surviving pig weights and lesions were similar in infected miniature and conventional pigs. the results indicate that the susceptibility of miniature pigs to i. suis is similar to that of conventional pigs. these findings should encourage their use as models for the study of neonatal co ...19911882501
experimental isospora suis infections in miniature swine.clinical responses to experimental isospora suis infections were compared in sinclair miniature pigs and cross-bred conventional pigs. pre-patent periods, fecal consistencies, oocyst excretion dynamics, trends in surviving pig weights and lesions were similar in infected miniature and conventional pigs. the results indicate that the susceptibility of miniature pigs to i. suis is similar to that of conventional pigs. these findings should encourage their use as models for the study of neonatal co ...19911882501
[lysosomal activity in enterocytes in the small intestine in piglets experimentally infected with isospora suis].the lysosomal activity of enterocytes of the small intestine mucosa was investigated in gnotobiotic and conventional piglets experimentally infected on the first day after birth (dab) by the oocysts of the coccidia isospora suis. a method of the proof of beta-d-glucuronidase (ec.3.2.1.31.) activity was used to demonstrate lysosomes. the piglets were infected by different infection doses of oocysts (100,000 oocysts in gnotobiotic piglets and 200,000 oocysts in conventional piglets). in the gnotob ...19911926681
[lysosomal activity in enterocytes in the small intestine in piglets experimentally infected with isospora suis].the lysosomal activity of enterocytes of the small intestine mucosa was investigated in gnotobiotic and conventional piglets experimentally infected on the first day after birth (dab) by the oocysts of the coccidia isospora suis. a method of the proof of beta-d-glucuronidase (ec.3.2.1.31.) activity was used to demonstrate lysosomes. the piglets were infected by different infection doses of oocysts (100,000 oocysts in gnotobiotic piglets and 200,000 oocysts in conventional piglets). in the gnotob ...19911926681
[density of selected enzymes in the goblet cells of the small intestine in piglets experimentally infected with the coccidium isospora suis].the density of selected enzymes in the goblet cells of the mucous membrane of the small intestine was studied in a group of 12 gnotobiotic piglets experimentally infected with the coccidium isospora suis one day after parturition (dpp), using the vickers m-786 scanning and integrating microdensity meter. at an infecting dose of 100,000 oocysts of i. suis, the histochemistry of the goblet cells of the mucous membrane of the piglets changed significantly in the period of 4 to 10 days after infecti ...19901978431
[density of selected enzymes in the goblet cells of the small intestine in piglets experimentally infected with the coccidium isospora suis].the density of selected enzymes in the goblet cells of the mucous membrane of the small intestine was studied in a group of 12 gnotobiotic piglets experimentally infected with the coccidium isospora suis one day after parturition (dpp), using the vickers m-786 scanning and integrating microdensity meter. at an infecting dose of 100,000 oocysts of i. suis, the histochemistry of the goblet cells of the mucous membrane of the piglets changed significantly in the period of 4 to 10 days after infecti ...19901978431
[densitometric analysis of aminopeptidase m activity in small intestine mucosa in piglets experimentally infected with isospora suis].in gnotobiotical and conventional piglets infected a day post partum (dpp) with oocysts of the coccidium isospora suis, densitometrical analysis of the activity of aminopeptidase m (ec.3.4.11.2; apm) was performed in the area of microvillous zone of the small intestine. piglets were infected with different infection doses of oocysts (100,000 oocysts) in gnotobiotical piglets and 200,000 oocysts in conventional piglets). in infected gnotobiotical piglets, the apm activity was studied in the perio ...19901982978
[densitometric analysis of aminopeptidase m activity in small intestine mucosa in piglets experimentally infected with isospora suis].in gnotobiotical and conventional piglets infected a day post partum (dpp) with oocysts of the coccidium isospora suis, densitometrical analysis of the activity of aminopeptidase m (ec.3.4.11.2; apm) was performed in the area of microvillous zone of the small intestine. piglets were infected with different infection doses of oocysts (100,000 oocysts) in gnotobiotical piglets and 200,000 oocysts in conventional piglets). in infected gnotobiotical piglets, the apm activity was studied in the perio ...19901982978
[histochemistry of selected peptidases in small intestine mucosa in piglets experimentally infected with isospora suis].in the small intestine mucosa of 24 gnotobiotical piglets experimentally infected the first day post partum with oocysts of the coccidium isospora suis, the activities of dipeptidylpeptidase iv (ec.3.4.14.5.; dap iv) and gamma-glutamyl transferase (ec.2.3.2.2.; ggt) in the microvillous zone of enterocytes were evaluated by scanning densitometry. the tissue of the small intestine in piglets infected with a dose of 100,000 oocysts of the coccidia of i. suis was examined in the period from the firs ...19901983128
[histochemistry of selected peptidases in small intestine mucosa in piglets experimentally infected with isospora suis].in the small intestine mucosa of 24 gnotobiotical piglets experimentally infected the first day post partum with oocysts of the coccidium isospora suis, the activities of dipeptidylpeptidase iv (ec.3.4.14.5.; dap iv) and gamma-glutamyl transferase (ec.2.3.2.2.; ggt) in the microvillous zone of enterocytes were evaluated by scanning densitometry. the tissue of the small intestine in piglets infected with a dose of 100,000 oocysts of the coccidia of i. suis was examined in the period from the firs ...19901983128
ultrastructure of developing isospora suis in cultured cells.the ultrastructure of isospora suis sporozoites, type-1 meronts, and type-1 merozoites was examined, using transmission electron microscopy of infected cultured cells. the ultrastructure of sporozoites and type-1 merozoites was similar. each possessed trimembranous pellicles, subpellicular microtubules, a conoid, anterior and posterior polar rings, rhoptries, micronemes, a single vesicular nucleus, tubular mitochondria, golgi complexes, ribosomes endoplasmic reticula, inactive micropores, amylop ...19912035924
ultrastructure of developing isospora suis in cultured cells.the ultrastructure of isospora suis sporozoites, type-1 meronts, and type-1 merozoites was examined, using transmission electron microscopy of infected cultured cells. the ultrastructure of sporozoites and type-1 merozoites was similar. each possessed trimembranous pellicles, subpellicular microtubules, a conoid, anterior and posterior polar rings, rhoptries, micronemes, a single vesicular nucleus, tubular mitochondria, golgi complexes, ribosomes endoplasmic reticula, inactive micropores, amylop ...19912035924
[alkaline phosphatase activity in goblet cells in the small intestine of piglets experimentally infected with the coccidium isospora suis].alkaline phosphatase activity (ec. 3.1.3.1.) in goblet cells was investigated in the small intestine of 16 gnotobiotic piglets infected one day after delivery (dad) by different rates of oocysts of isospora suis coccidia. at a high infection rate of i. suis (750,000) the goblet cells were found to be highly positive to alkaline phosphatase on day 3 to day 4 after infection (dai). in piglets infected by a low infection rate of i. suis oocysts (100,000) the activity of alkaline phosphatase activit ...19902219627
[alkaline phosphatase activity in goblet cells in the small intestine of piglets experimentally infected with the coccidium isospora suis].alkaline phosphatase activity (ec. 3.1.3.1.) in goblet cells was investigated in the small intestine of 16 gnotobiotic piglets infected one day after delivery (dad) by different rates of oocysts of isospora suis coccidia. at a high infection rate of i. suis (750,000) the goblet cells were found to be highly positive to alkaline phosphatase on day 3 to day 4 after infection (dai). in piglets infected by a low infection rate of i. suis oocysts (100,000) the activity of alkaline phosphatase activit ...19902219627
double alteration of the small intestine in conventional and gnotobiotic piglets experimentally infected with the coccidium isospora suis (apicomplexa, eimeriidae).the pathogenicity of the coccidium isospora suis was studied in 42 conventional and 26 gnotobiotic piglets at 1-13 days post infection (dpi). the prepatent period of the coccidium i. suis was 4.5-5 days. only in one experiment in conventional piglets the prepatent period of 3.5 days was recorded. at least 9 days pathogenesis of i. suis was recorded during which double alteration of the small intestine occurred. the first alteration was found at 3-4 dpi. the second phase of alterative changes was ...19902332196
double alteration of the small intestine in conventional and gnotobiotic piglets experimentally infected with the coccidium isospora suis (apicomplexa, eimeriidae).the pathogenicity of the coccidium isospora suis was studied in 42 conventional and 26 gnotobiotic piglets at 1-13 days post infection (dpi). the prepatent period of the coccidium i. suis was 4.5-5 days. only in one experiment in conventional piglets the prepatent period of 3.5 days was recorded. at least 9 days pathogenesis of i. suis was recorded during which double alteration of the small intestine occurred. the first alteration was found at 3-4 dpi. the second phase of alterative changes was ...19902332196
[activity of mucosubstances and the goblet cell count in the large intestine of piglets infected with the coccidium, isospora suis].in a group of conventional and gnotobiotic piglets experimentally infected with the isospora suis coccidia the quantitative presence of acid and neutral mucous substances in the large intestine and the counts of goblet cells in the surface mucosa and in lieberkühnis crypts (in the following text called just the crypts) were investigated. in conventional piglets infected with the dose of 200,000 oocysts of i. suis coccidia the lowest content of acid mucous substances was recorded from the eighth ...19902333678
[activity of mucosubstances and the goblet cell count in the large intestine of piglets infected with the coccidium, isospora suis].in a group of conventional and gnotobiotic piglets experimentally infected with the isospora suis coccidia the quantitative presence of acid and neutral mucous substances in the large intestine and the counts of goblet cells in the surface mucosa and in lieberkühnis crypts (in the following text called just the crypts) were investigated. in conventional piglets infected with the dose of 200,000 oocysts of i. suis coccidia the lowest content of acid mucous substances was recorded from the eighth ...19902333678
[morphometric analysis of the activity of alkaline phosphatase in the small intestine of piglets infected with the coccidium isospora suis].gnotobiotical one-day-old piglets were infected with 100,000 isospora suis coccidia oocysts, and were immediately killed. in piglets killed on the 3rd to 11th day after infection (dai), the morphometric analysis of alkaline phosphatase activity was performed in the area of the microvillus zone of small intestine. in the control 2-7 days old animals, the small intestine was not equally supplied with alkaline phosphatase. in duodenum the activity reached 88.37 per cent of the active length of abso ...19902375068
[morphometric analysis of the activity of alkaline phosphatase in the small intestine of piglets infected with the coccidium isospora suis].gnotobiotical one-day-old piglets were infected with 100,000 isospora suis coccidia oocysts, and were immediately killed. in piglets killed on the 3rd to 11th day after infection (dai), the morphometric analysis of alkaline phosphatase activity was performed in the area of the microvillus zone of small intestine. in the control 2-7 days old animals, the small intestine was not equally supplied with alkaline phosphatase. in duodenum the activity reached 88.37 per cent of the active length of abso ...19902375068
[the ratio of enterocytes and goblet cells in the mucosa of the small intestine in experimental infection of piglets with the coccidium isospora suis].the proportions of cup-shaped cells and enterocytes were studied in piglets infected the first and fifth day after birth with 200,000 oocysts of isospora suis. the greatest imbalance was found in the ratio of the cells of the intestinal mucous membrane in the region of middle and lower jejunum (ratio of 10:1.75)--this was recorded the third to fourth day after infection when the ratio made 10:0.62, whereas in the control animals it was 10:5.7. on the eighth to ninth day after infection the numbe ...19892494785
[the ratio of enterocytes and goblet cells in the mucosa of the small intestine in experimental infection of piglets with the coccidium isospora suis].the proportions of cup-shaped cells and enterocytes were studied in piglets infected the first and fifth day after birth with 200,000 oocysts of isospora suis. the greatest imbalance was found in the ratio of the cells of the intestinal mucous membrane in the region of middle and lower jejunum (ratio of 10:1.75)--this was recorded the third to fourth day after infection when the ratio made 10:0.62, whereas in the control animals it was 10:5.7. on the eighth to ninth day after infection the numbe ...19892494785
[mucus synthesis in the goblet cells of the small intestine in experimental infection with the coccidium isospora suis in piglets].the state of mucus synthesis in the goblet cells of the small intestine was studied in conventional piglets infected with a dose of 200,000 oocytes of the coccidium isospora suis the first and fifth day after parturition. the synthesis of mucus and its chemical characteristics undergo significant changes during the third and fourth day after infection. the activity of acid and neutral mucous substances declines; their level and the physiological synthetic function of goblet cells begin to return ...19892631376
[mucus synthesis in the goblet cells of the small intestine in experimental infection with the coccidium isospora suis in piglets].the state of mucus synthesis in the goblet cells of the small intestine was studied in conventional piglets infected with a dose of 200,000 oocytes of the coccidium isospora suis the first and fifth day after parturition. the synthesis of mucus and its chemical characteristics undergo significant changes during the third and fourth day after infection. the activity of acid and neutral mucous substances declines; their level and the physiological synthetic function of goblet cells begin to return ...19892631376
[alkaline phosphatase activity levels during infection with the coccidia isospora suis in piglets].the activity of alkaline phosphatase (ec 3.1.3.1.) was studied in the small intestines of piglets infected with the coccidia isospora suis on the first and fifth day of age. alkaline phosphatase activity is reduced significantly the third to fourth day after infection (dai) throughout the small intestine in piglets infected on the fifth day of age. similar results were obtained in the piglets killed the eighth to tenth dai which had also been infected on the fifth day of their age. piglets infec ...19892728264
[alkaline phosphatase activity levels during infection with the coccidia isospora suis in piglets].the activity of alkaline phosphatase (ec 3.1.3.1.) was studied in the small intestines of piglets infected with the coccidia isospora suis on the first and fifth day of age. alkaline phosphatase activity is reduced significantly the third to fourth day after infection (dai) throughout the small intestine in piglets infected on the fifth day of age. similar results were obtained in the piglets killed the eighth to tenth dai which had also been infected on the fifth day of their age. piglets infec ...19892728264
[activity of selected dehydrogenases and monoamine oxidases in the small intestine of gnotobiotic piglets infected with the coccidium isospora suis].in the small intestine of 16 gnotobiotic piglets infected a day post partum (dpp) by isospora suis coccidia the activities were studied of selected dehydrogenases and monoaminoxidase (o2 oxidoreductase, maox, ec 1.4.3.4.). the following dehydrogenases were investigated: succinate dehydrogenase (sdh, ec 1.3.99.1.), glycerol-3-phosphate dehydrogenase (glycerol-3-phosphate:menadion oxidoreductase, gpox, ec 1.1.99.5.) and tetrazolium oxidoreductase (nadh, es 1.6.99.3.). the activities of nadh and gp ...19892756618
[activity of selected dehydrogenases and monoamine oxidases in the small intestine of gnotobiotic piglets infected with the coccidium isospora suis].in the small intestine of 16 gnotobiotic piglets infected a day post partum (dpp) by isospora suis coccidia the activities were studied of selected dehydrogenases and monoaminoxidase (o2 oxidoreductase, maox, ec 1.4.3.4.). the following dehydrogenases were investigated: succinate dehydrogenase (sdh, ec 1.3.99.1.), glycerol-3-phosphate dehydrogenase (glycerol-3-phosphate:menadion oxidoreductase, gpox, ec 1.1.99.5.) and tetrazolium oxidoreductase (nadh, es 1.6.99.3.). the activities of nadh and gp ...19892756618
[the observation of coccidia in swine].the coccidia species of domestic pig and wild boar were compared and defined morphologically and the sporogony of both was examined. no difference could be detected between the coccidia species of domestic pig and wild boar. six eimeria and one isospora species were found: eimeria debliecki, e. polita, e. porci, e. scabra, e. spinosa, e. suis and isospora suis. e porci could be found in austria for the first time. e. cerdonis vetterling, 1965 is a synonym of e. polita pellérdy, 1949.19892782664
[the observation of coccidia in swine].the coccidia species of domestic pig and wild boar were compared and defined morphologically and the sporogony of both was examined. no difference could be detected between the coccidia species of domestic pig and wild boar. six eimeria and one isospora species were found: eimeria debliecki, e. polita, e. porci, e. scabra, e. spinosa, e. suis and isospora suis. e porci could be found in austria for the first time. e. cerdonis vetterling, 1965 is a synonym of e. polita pellérdy, 1949.19892782664
[coccidiosis in suckling calves raised on large farms].in a large herd of pigs whose piglets suffered from diarrheas coprological examination revealed the great incidence of isospora suis coccidia in piglets. the first oocysts were found out in seven-day piglets; the highest incidence was observed in twelve-day piglets (65.9%); the time of oocyst elimination (patency) was five to six days. the oocysts appeared in the excrements of piglets two to three days after the diarrhea onset. individual examinations of piglets demonstrated the correlation betw ...19863101268
[coccidiosis in suckling calves raised on large farms].in a large herd of pigs whose piglets suffered from diarrheas coprological examination revealed the great incidence of isospora suis coccidia in piglets. the first oocysts were found out in seven-day piglets; the highest incidence was observed in twelve-day piglets (65.9%); the time of oocyst elimination (patency) was five to six days. the oocysts appeared in the excrements of piglets two to three days after the diarrhea onset. individual examinations of piglets demonstrated the correlation betw ...19863101268
serum proteins of neonatal pigs orally inoculated with isospora suis oocysts.serum proteins were evaluated by agarose electrophoresis at periodic intervals between postinoculation days (pid) 2 and 35 in 4 litters of pigs inoculated orally at 36 to 38 hours of age with 70,000 sporulated isospora suis oocysts and in 4 litters of age-matched noninoculated control pigs. in inoculated pigs, clinical disease characterized by vomiting and diarrhea began at pid 3 to 4 and was resolved by pid 11. isospora suis oocysts were detected in feces of inoculated pigs from pid 5 to 25, wi ...19883358549
serum proteins of neonatal pigs orally inoculated with isospora suis oocysts.serum proteins were evaluated by agarose electrophoresis at periodic intervals between postinoculation days (pid) 2 and 35 in 4 litters of pigs inoculated orally at 36 to 38 hours of age with 70,000 sporulated isospora suis oocysts and in 4 litters of age-matched noninoculated control pigs. in inoculated pigs, clinical disease characterized by vomiting and diarrhea began at pid 3 to 4 and was resolved by pid 11. isospora suis oocysts were detected in feces of inoculated pigs from pid 5 to 25, wi ...19883358549
isospora suis in pigs with post weaning diarrhoea. 19883381428
isospora suis in pigs with post weaning diarrhoea. 19883381428
[pathomorphological changes in the small intestine of pigs with rotavirus diarrhea].studied were the pathohistological changes and the morphologic-structural injuries of intestinal mucosa of pigs (5-7 days old), spontaneously affected with rotavirus diarrhea, raised by traditional methods. simultaneously with the rotavirus invasion was established isospora suis. in spite of the early time of the disorder (the test was carried out 24 hours after the appearance of the first clinical symptoms) there were established different in degree and expression morphologic changes; atrophy a ...19873445448
[pathomorphological changes in the small intestine of pigs with rotavirus diarrhea].studied were the pathohistological changes and the morphologic-structural injuries of intestinal mucosa of pigs (5-7 days old), spontaneously affected with rotavirus diarrhea, raised by traditional methods. simultaneously with the rotavirus invasion was established isospora suis. in spite of the early time of the disorder (the test was carried out 24 hours after the appearance of the first clinical symptoms) there were established different in degree and expression morphologic changes; atrophy a ...19873445448
reduced pre-patent period in experimental infection of piglets with the coccidium isospora suis. 19873583128
reduced pre-patent period in experimental infection of piglets with the coccidium isospora suis. 19873583128
development of isospora suis from pigs in primary porcine and bovine cell cultures.sporozoites of isospora suis penetrated and developed by endodyogeny in primary porcine kidney (ppk) and primary fetal bovine kidney (pfbk) cell cultures. motile merozoites and binucleate type i meronts were observed in both types of cultured cells. multinucleate type ii meronts developed in ppk cell cultures only. these multinucleate meronts were always found singly, were nonmotile and did not form merozoites.19873617433
development of isospora suis from pigs in primary porcine and bovine cell cultures.sporozoites of isospora suis penetrated and developed by endodyogeny in primary porcine kidney (ppk) and primary fetal bovine kidney (pfbk) cell cultures. motile merozoites and binucleate type i meronts were observed in both types of cultured cells. multinucleate type ii meronts developed in ppk cell cultures only. these multinucleate meronts were always found singly, were nonmotile and did not form merozoites.19873617433
pathology of natural isosporosis in nursing piglets.in piglets suffering from natural coccidiosis, post-mortem examination showed that pathological changes induced by isospora suis were evident from day 7 to day 14 of life, and particularly, by days 9 and 10. macroscopically, the changes were manifest as enteritis varying from catarrhal to pseudomembraneous form. microscopically, they consisted of more or less extensive atrophy of villi whose apical parts were necrotic, of metaplasia and erosion of epithelium. with the exception of duodenum and t ...19873666610
pathology of natural isosporosis in nursing piglets.in piglets suffering from natural coccidiosis, post-mortem examination showed that pathological changes induced by isospora suis were evident from day 7 to day 14 of life, and particularly, by days 9 and 10. macroscopically, the changes were manifest as enteritis varying from catarrhal to pseudomembraneous form. microscopically, they consisted of more or less extensive atrophy of villi whose apical parts were necrotic, of metaplasia and erosion of epithelium. with the exception of duodenum and t ...19873666610
parasite transmission in confined hogs.examinations of fecal specimens from swine of all ages maintained in a totally enclosed confinement facility in south georgia were conducted in a 5-year survey (1977-1981) to determine the prevalence, intensity, and transmission patterns of intestinal nematode and protozoan parasites. weaned pigs in the nursery had no detectable parasitic infections except sporadic isospora suis in newly weaned pigs. growing-finishing hogs had a low prevalence of ascaris suum, oesophagostomum spp. and balantidiu ...19863705423
parasite transmission in confined hogs.examinations of fecal specimens from swine of all ages maintained in a totally enclosed confinement facility in south georgia were conducted in a 5-year survey (1977-1981) to determine the prevalence, intensity, and transmission patterns of intestinal nematode and protozoan parasites. weaned pigs in the nursery had no detectable parasitic infections except sporadic isospora suis in newly weaned pigs. growing-finishing hogs had a low prevalence of ascaris suum, oesophagostomum spp. and balantidiu ...19863705423
porcine coccidia in papua new guinea.faecal samples from 232 domestic pigs raised on concrete, 98 free-ranging village pigs, and five wild boar showed 46.6 (108/232), 54 (53/98) and 80% (4/5) prevalence of coccidian oocysts, respectively. eight species of eimeria, and isospora suis, were recovered. in their descending order of predominance in the pigs raised on concrete, the species of coccidia were e. debliecki (26.7%), e. scabra (22.4%), e. neodebliecki (19.8%), e. porci (15.5%), e. suis (11.6%), e. polita (8.6%), e. perminuta (7 ...19863727342
porcine coccidia in papua new guinea.faecal samples from 232 domestic pigs raised on concrete, 98 free-ranging village pigs, and five wild boar showed 46.6 (108/232), 54 (53/98) and 80% (4/5) prevalence of coccidian oocysts, respectively. eight species of eimeria, and isospora suis, were recovered. in their descending order of predominance in the pigs raised on concrete, the species of coccidia were e. debliecki (26.7%), e. scabra (22.4%), e. neodebliecki (19.8%), e. porci (15.5%), e. suis (11.6%), e. polita (8.6%), e. perminuta (7 ...19863727342
the sporulation time of isospora suis oocysts from different sources.feces containing isospora suis oocysts were collected from naturally- and experimentally-infected pigs from four different areas of the united states. the unsporulated oocysts were cleaned, concentrated, mixed with 2.5% aqueous potassium dichromate solution, poured into petri dishes to a depth of 5 mm, and incubated at 25 degrees c. the oocysts were examined with a microscope at 12 h intervals and the stages of sporulation present were counted. although a few oocysts were completely sporulated a ...19863788016
the sporulation time of isospora suis oocysts from different sources.feces containing isospora suis oocysts were collected from naturally- and experimentally-infected pigs from four different areas of the united states. the unsporulated oocysts were cleaned, concentrated, mixed with 2.5% aqueous potassium dichromate solution, poured into petri dishes to a depth of 5 mm, and incubated at 25 degrees c. the oocysts were examined with a microscope at 12 h intervals and the stages of sporulation present were counted. although a few oocysts were completely sporulated a ...19863788016
interfering effect of isospora suis infection on salmonella typhimurium infection in swine.piglets were each orally inoculated daily for 4 consecutive days, with 10(4)-10(5) nalidixic acid-resistant salmonella typhimurium and, starting 1 day after inoculation, with 50 000 isospora suis sporulated oocysts. control pigs were given s. typhimurium alone following the same inoculation schedule. fecal samples were taken 5, 7 and 10 days after the coccidial inoculation, and all the pigs were necropsied 13 days after inoculation. s. typhimurium counts in the feces and in the cecal contents an ...19853890353
interfering effect of isospora suis infection on salmonella typhimurium infection in swine.piglets were each orally inoculated daily for 4 consecutive days, with 10(4)-10(5) nalidixic acid-resistant salmonella typhimurium and, starting 1 day after inoculation, with 50 000 isospora suis sporulated oocysts. control pigs were given s. typhimurium alone following the same inoculation schedule. fecal samples were taken 5, 7 and 10 days after the coccidial inoculation, and all the pigs were necropsied 13 days after inoculation. s. typhimurium counts in the feces and in the cecal contents an ...19853890353
pathogenicity of isospora suis in gnotobiotic and conventionalised piglets.isospora suis is unequivocally a primary pathogen of swine. inoculation of i suis in conventionalised and germ-free piglets caused a biphasic disease course with marked diarrhoea, villous atrophy and necrosis of the intestinal epithelium at four to six and eight to 10 days after inoculation. the presence of a normal bacterial flora markedly (p less than 0.05) influenced the survival rate of piglets but did not appear to affect the histopathological changes observed. mild limited focal necrosis a ...19854024419
pathogenicity of isospora suis in gnotobiotic and conventionalised piglets.isospora suis is unequivocally a primary pathogen of swine. inoculation of i suis in conventionalised and germ-free piglets caused a biphasic disease course with marked diarrhoea, villous atrophy and necrosis of the intestinal epithelium at four to six and eight to 10 days after inoculation. the presence of a normal bacterial flora markedly (p less than 0.05) influenced the survival rate of piglets but did not appear to affect the histopathological changes observed. mild limited focal necrosis a ...19854024419
effects of experimentally induced isospora suis infection on morbidity, mortality, and weight gains in nursing pigs.forty-nine pigs from 6 litters were inoculated at 3 days of age with 300,000 sporulated oocysts of isospora suis to determine the effects of neonatal coccidiosis on morbidity, mortality, and weight gain in nursing pigs. fifty-one control pigs from 6 litters were not inoculated. three to 5 days after inoculation, the inoculated pigs developed a nonhemorrhagic diarrhea, generally lasting 6 to 10 days, that lead to visible dehydration, loss of condition, and 20.4% mortality. control pigs did not di ...19854026033
effects of experimentally induced isospora suis infection on morbidity, mortality, and weight gains in nursing pigs.forty-nine pigs from 6 litters were inoculated at 3 days of age with 300,000 sporulated oocysts of isospora suis to determine the effects of neonatal coccidiosis on morbidity, mortality, and weight gain in nursing pigs. fifty-one control pigs from 6 litters were not inoculated. three to 5 days after inoculation, the inoculated pigs developed a nonhemorrhagic diarrhea, generally lasting 6 to 10 days, that lead to visible dehydration, loss of condition, and 20.4% mortality. control pigs did not di ...19854026033
experimental coccidiosis (isospora suis) in a litter of feral piglets. 19854032631
experimental coccidiosis (isospora suis) in a litter of feral piglets. 19854032631
isospora suis species in a danish spf-herd.in two studies carried out in 1974-75 on 10 eight and 25 two week old piglets obtained from a spf-herd the endoparasitic status was determined. samples of faeces were examined by microscopy which revealed the presence of isospora suis, a finding that was quite unexpected in a spf-herd. during days 17-23 pp. the 25 piglets were not found to excrete oocysts, the earliest findings of oocysts being made 24-35 days p.p. the 10 piglets being kept isolated in individual pens excreted oocysts up until 8 ...19854034389
isospora suis species in a danish spf-herd.in two studies carried out in 1974-75 on 10 eight and 25 two week old piglets obtained from a spf-herd the endoparasitic status was determined. samples of faeces were examined by microscopy which revealed the presence of isospora suis, a finding that was quite unexpected in a spf-herd. during days 17-23 pp. the 25 piglets were not found to excrete oocysts, the earliest findings of oocysts being made 24-35 days p.p. the 10 piglets being kept isolated in individual pens excreted oocysts up until 8 ...19854034389
control of isospora suis-induced coccidiosis on a swine farm.results of a program designed to control neonatal porcine coccidiosis on a total confinement, farrow-to-finish swine farm are reported. the control program consisted of washing, phenol disinfection, and steam cleaning of farrowing houses and treatment of sows with amprolium hcl before and after farrowing. before initiation of the control program, 88.9% of the sows examined in the farrowing house were negative for coccidian oocysts, 9.9% were positive for eimeria spp, and 1.2% were positive for i ...19854039545
control of isospora suis-induced coccidiosis on a swine farm.results of a program designed to control neonatal porcine coccidiosis on a total confinement, farrow-to-finish swine farm are reported. the control program consisted of washing, phenol disinfection, and steam cleaning of farrowing houses and treatment of sows with amprolium hcl before and after farrowing. before initiation of the control program, 88.9% of the sows examined in the farrowing house were negative for coccidian oocysts, 9.9% were positive for eimeria spp, and 1.2% were positive for i ...19854039545
experimental transmission of intestinal coccidiosis to piglets: clinical, parasitological and pathological findings.twenty-eight piglets coming from a "specific pathogen free" herd were inoculated at three days of age with 50 000 or 100 000 sporulated oocysts of isospora suis. fecal samples were examined for oocyst shedding daily and several clinical parameters were recorded. ten piglets were used as normal controls. groups of piglets were euthanized from three days to 12 days postinoculation and routine necropsies were performed. bacteriological, virological, parasitological and histopathological examination ...19836230142
experimental transmission of intestinal coccidiosis to piglets: clinical, parasitological and pathological findings.twenty-eight piglets coming from a "specific pathogen free" herd were inoculated at three days of age with 50 000 or 100 000 sporulated oocysts of isospora suis. fecal samples were examined for oocyst shedding daily and several clinical parameters were recorded. ten piglets were used as normal controls. groups of piglets were euthanized from three days to 12 days postinoculation and routine necropsies were performed. bacteriological, virological, parasitological and histopathological examination ...19836230142
field study of coccidial and rotaviral diarrhoea in unweaned piglets.a study of diarrhoea in unweaned piglets was carried out in nine herds, with special reference to the enteropathogenic agents which could be demonstrated. coccidial (isospora suis) and rotaviral infections were both identified, either singly or in combination. more extensive studies of i suis infection were undertaken in two of the herds and it was found that diarrhoea occurred most commonly in five- to 14-day-old piglets. piglets with i suis infection were not necessarily diarrhoeic but grew po ...19826278696
field study of coccidial and rotaviral diarrhoea in unweaned piglets.a study of diarrhoea in unweaned piglets was carried out in nine herds, with special reference to the enteropathogenic agents which could be demonstrated. coccidial (isospora suis) and rotaviral infections were both identified, either singly or in combination. more extensive studies of i suis infection were undertaken in two of the herds and it was found that diarrhoea occurred most commonly in five- to 14-day-old piglets. piglets with i suis infection were not necessarily diarrhoeic but grew po ...19826278696
neonatal diarrhea of pigs in quebec: infectious causes of significant outbreaks.to evaluate the relative importance of the various enteropathogens causing neonatal diarrhea in quebec farrowing operations, observations were made on 749 diarrheic pigs from 325 outbreaks of diarrhea. they were one to 15 days of age, and were obtained alive for necropsy generally within 48 hours of the onset of diarrhea. some pigs were from severe, explosive outbreaks of diarrhea with high morbidity and mortality rates, while others were from herds with chronic neonatal diarrhea with lower morb ...19836299483
neonatal diarrhea of pigs in quebec: infectious causes of significant outbreaks.to evaluate the relative importance of the various enteropathogens causing neonatal diarrhea in quebec farrowing operations, observations were made on 749 diarrheic pigs from 325 outbreaks of diarrhea. they were one to 15 days of age, and were obtained alive for necropsy generally within 48 hours of the onset of diarrhea. some pigs were from severe, explosive outbreaks of diarrhea with high morbidity and mortality rates, while others were from herds with chronic neonatal diarrhea with lower morb ...19836299483
epidemiology of porcine neonatal steatorrhoea in sweden. 1. prevalence and clinical significance of coccidal and rotaviral infections.a study of neonatal steatorrhoea in unweaned piglets was carried out in randomly visited herds. isospora suis and rotavirus were shown to be incriminated in this type of enteric disease and these agents may be considered important enteropathogens for 1-3-week-old piglets in sweden. more extensive studies of i. suis were undertaken in one large herd. it was found that steatorrhoea may appear in 4-day-old piglets but oocysts were not detected until some days latter. piglets that were shedding i. s ...19846330668
epidemiology of porcine neonatal steatorrhoea in sweden. 1. prevalence and clinical significance of coccidal and rotaviral infections.a study of neonatal steatorrhoea in unweaned piglets was carried out in randomly visited herds. isospora suis and rotavirus were shown to be incriminated in this type of enteric disease and these agents may be considered important enteropathogens for 1-3-week-old piglets in sweden. more extensive studies of i. suis were undertaken in one large herd. it was found that steatorrhoea may appear in 4-day-old piglets but oocysts were not detected until some days latter. piglets that were shedding i. s ...19846330668
isospora suis infection in piglets. a review.recently several reports have been published on the association of isospora suis with diarrhea in piglets. a review is given of i. suis infection in piglets, in which the taxonomy, life cycle, pathogenicity, immunity, and treatment of i. suis and related organisms are discussed.19836359663
isospora suis infection in piglets. a review.recently several reports have been published on the association of isospora suis with diarrhea in piglets. a review is given of i. suis infection in piglets, in which the taxonomy, life cycle, pathogenicity, immunity, and treatment of i. suis and related organisms are discussed.19836359663
prevalence of oocysts of isospora suis and eimeria spp from sows on farms with and without a history of neonatal coccidiosis.oocysts of isospora suis were not found in any of 77 fecal samples from sows on farms with a history of neonatal coccidiosis. oocysts of isospora suis were found in 1 of 172 fecal samples from farms without a history of neonatal coccidiosis. oocysts of eimeria spp were found in 81.8% of the sows from farms with a history of neonatal coccidiosis and in 94.8% of the sows from farms without a history of neonatal coccidiosis. oocysts of isospora spp from birds were encountered as a pseudoparasite in ...19846469839
prevalence of oocysts of isospora suis and eimeria spp from sows on farms with and without a history of neonatal coccidiosis.oocysts of isospora suis were not found in any of 77 fecal samples from sows on farms with a history of neonatal coccidiosis. oocysts of isospora suis were found in 1 of 172 fecal samples from farms without a history of neonatal coccidiosis. oocysts of eimeria spp were found in 81.8% of the sows from farms with a history of neonatal coccidiosis and in 94.8% of the sows from farms without a history of neonatal coccidiosis. oocysts of isospora spp from birds were encountered as a pseudoparasite in ...19846469839
life cycle of isospora suis in gnotobiotic and conventionalized piglets.isospora suis had 3 asexual and 1 sexual intra-intestinal conventional life cycle. the first asexual generation was most prominent at 2 days p.i. (post inoculation) and produced 2-7 merozoites. the second-generation meronts were prevalent at 3-4 days p.i. and produced 2-12 large merozoites. at 4-5 days p.i. the third generation meronts were prominent and produced 4-24 small crescent shaped merozoites. mature sexual stages were most prominent at 5-6 days p.i. the stages were most numerous in the ...19846543059
life cycle of isospora suis in gnotobiotic and conventionalized piglets.isospora suis had 3 asexual and 1 sexual intra-intestinal conventional life cycle. the first asexual generation was most prominent at 2 days p.i. (post inoculation) and produced 2-7 merozoites. the second-generation meronts were prevalent at 3-4 days p.i. and produced 2-12 large merozoites. at 4-5 days p.i. the third generation meronts were prominent and produced 4-24 small crescent shaped merozoites. mature sexual stages were most prominent at 5-6 days p.i. the stages were most numerous in the ...19846543059
[cryptosporidium sp. and isospora suis in swine in italy].the presence of cryptosporidium sp. and isospora suis is reported for the first time in piglets in italy, in the province of mantova: the first one has been found in 1 and the second one in 39 out of 200 litters. two piglets, respectively 20-day-old and 24-day-old, were experimentally infected with cryptosporidium sp. oocysts from naturally infected calves, without clinical signs.19846600003
[cryptosporidium sp. and isospora suis in swine in italy].the presence of cryptosporidium sp. and isospora suis is reported for the first time in piglets in italy, in the province of mantova: the first one has been found in 1 and the second one in 39 out of 200 litters. two piglets, respectively 20-day-old and 24-day-old, were experimentally infected with cryptosporidium sp. oocysts from naturally infected calves, without clinical signs.19846600003
motility of isospora suis meronts. 19836631645
motility of isospora suis meronts. 19836631645
complete development of isopora suis of swine in chicken embryos.development of the swine coccidium , isospora suis, in embryonated chicken eggs is described. the allantoic cavities of eight-to-ten-day-old white leghorn embryos were inoculated with either 100,000 or 200,000 sporozoites. developmental stages morphologically similar to those found in the intestines of piglets were present in the endodermal layer of the chorioallantoic membrane (cam), beginning three days post inoculation (pi). no stages were found in the mesodermal or ectodermal layers of the c ...19846737316
complete development of isopora suis of swine in chicken embryos.development of the swine coccidium , isospora suis, in embryonated chicken eggs is described. the allantoic cavities of eight-to-ten-day-old white leghorn embryos were inoculated with either 100,000 or 200,000 sporozoites. developmental stages morphologically similar to those found in the intestines of piglets were present in the endodermal layer of the chorioallantoic membrane (cam), beginning three days post inoculation (pi). no stages were found in the mesodermal or ectodermal layers of the c ...19846737316
excystation of isospora suis biester, 1934 of swine.the in vitro excystation of sporozoites of isospora suis biester 1934 is described. sporocysts of i. suis lack a stieda body. upon incubation in 0.75% sodium taurocholate or in 0.25% trypsin + 0.75% sodium taurocholate excystation solutions, sporozoites were released by separation of the sporocyst wall into four plates. occasionally, the sporocyst wall did not separate completely but opened partially and released the sporozoite. at the time of excystation, sporozoites were short and broad but be ...19836837100
excystation of isospora suis biester, 1934 of swine.the in vitro excystation of sporozoites of isospora suis biester 1934 is described. sporocysts of i. suis lack a stieda body. upon incubation in 0.75% sodium taurocholate or in 0.25% trypsin + 0.75% sodium taurocholate excystation solutions, sporozoites were released by separation of the sporocyst wall into four plates. occasionally, the sporocyst wall did not separate completely but opened partially and released the sporozoite. at the time of excystation, sporozoites were short and broad but be ...19836837100
coccidiosis in swine: dose and age response to isospora suis.coccidiosis is a disease of the young piglet due to infection with isospora suis and is characterized by diarrhea which is nonresponsive to antibacterial therapy. there is variable morbidity and mortality. piglets develop a more severe clinical illness and enteritis when infected with i. suis at one to three days of age than when infected at two weeks of age. microscopic lesions range from villous atrophy and mild erosion to severe fibrinonecrotic enteritis.19826889908
coccidiosis in swine: dose and age response to isospora suis.coccidiosis is a disease of the young piglet due to infection with isospora suis and is characterized by diarrhea which is nonresponsive to antibacterial therapy. there is variable morbidity and mortality. piglets develop a more severe clinical illness and enteritis when infected with i. suis at one to three days of age than when infected at two weeks of age. microscopic lesions range from villous atrophy and mild erosion to severe fibrinonecrotic enteritis.19826889908
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