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echinostoma revolutum: freshwater snails as the second intermediate hosts in chiang mai, thailand.the occurrence of 37-collar spined echinostome metacercariae in freshwater snails was investigated in 6 districts of chiang mai province, thailand, from october 2011 to april 2012. a total of 2,914 snails that belong to 12 species were examined, and 7 snail species (clea helena, eyriesia eyriesi, bithynia funiculata, bithynia siamensis siamensis, filopaludina doliaris, filopaludina sumatrensis polygramma, and filopaludina martensi martensi) were found infected with echinostome metacercariae. the ...201323710085
genetic markers for studies on the systematics and population genetics of snails, bithynia spp., the first intermediate hosts of opisthorchis viverrini in thailand.snails are the critical amplifying hosts of the liver fluke opisthorchis viverrini, the causative agent of hepatobiliary disease and cholangiocarcinoma in the mekong area of southeast asia. bithynia funiculata, b. siamensis goniomphalos and b. s. siamensis are the first intermediate hosts of o. viverrini in thailand. morphological similarity between bithynia species and subspecies creates problems for their taxonomic identification and an understanding of bithynia systematics. in this study, mul ...201121352793
studies on concomitant antigens of bithynia funiculata for detection of antibody to opisthorchis viverrini: effect of different centrifugal speeds on antigen preparation.antigens derived from somatic extracts of bithynia funiculata, an intermediate snail host of o. viverrini, have been demonstrated to be highly heterogeneous in molecular weight (mw). these antigens have been suggested to be of potential use for serodiagnosis. in this study, b. funiculata somatic antigens were extracted using five different centrifugal speeds, namely 10,000 (c1); 20,000 (c2); 30,000 (c3); 40,000 (c4) and 50,000 (c5) rpm, with the aim of removing some non-specific antigens and det ...200319230581
field investigation of bithynia funiculata, intermediate host of opisthorchis viverrini in northern thailand.a survey of freshwater snail, bithynia funiculata, was conducted in four locations, doi saket, mueang, saraphi and mae rim districts of chiang mai province, northern thailand, between june and october, during the rainy season of 2004. a total of 2,240 snails was collected and classified into 7 families and 15 genera; of which 352 b. funiculata were obtained. b. funiculata was found most abundant in july and september. the infection rate of trematode cercariae in b. funiculata was 9.6% (19/352), ...200617121291
evaluation of bithynia funiculata snail antigens by elisa-serodiagnosis of human opisthorchiasis.four batches of crude somatic antigens from: (1) opisthorchis viverrini adult worms, (2) bithynia funiculata-whole body, (3) b. funiculata-head-foot, and (4) b. funiculata-visceral mass were assayed against sera from 81 opisthorchiasis patients, 30 parasite-free healthy individuals, and 50 individuals infected with other helminthic infections, and their antibody levels determined. by igg-elisa, the antigenic reactive proteins were found in both the head-foot and the visceral mass of b. funiculat ...19979561614
detection of shared antigens of human liver flukes opisthorchis viverrini and its snail host, bithynia spp.the relationship between opisthorchis viverrini and its snail intermediate host including bithynia funiculata, b. siamensis siamensis and b. siamensis goniomphalos was carried out on the correlation of shared antigens and infection rates. b. funiculata and b. s. siamensis were equally susceptible to o. viverrini with relatively high infection rates of 72.2% and 69.9% respectively whereas b. s. goniomphalos gave lowest percentage of infection of only 9.6%. by immunoelectrophoresis, crude extract ...19902075387
laboratory studies on host-parasite relationship of bithynia snails and the liver fluke, opisthorchis viverrini.the infection rate of bithynia snails to opisthorchis viverrini eggs was studied in relation to exposure intensity, age and species of host. it was found that 50 miracidial eggs per snail yielded the highest percentage of living surviving positive snails. bithynia funiculata and bithynia siamensis siamensis were highly susceptible to o. viverrini, about four to seven times higher than bithynia siamensis goniomphalos. young snails, 1-3 months old, appeared more susceptible than old snails.19911948284
species-specific primers designed from rapd products for bithynia funiculata, the first intermediate host of liver fluke, opisthorchis viverrini, in north thailand.bithynia funiculata is the first intermediate host of the human liver fluke opisthorchis viverrini in northern thailand but its identification through morphological analysis is often problematic due to the shortage of gastropod taxonomists. as a consequence, we focused on the development of species-specific primers for use as an identification tool. our work involved recovery of a 502-base pair (bp) amplicon of unknown function through species-specific primers whose effectiveness was tested by a ...201323157281
opisthorchis viverrini infection in the snail and fish intermediate hosts in central vietnam.opisthorchis viverrini, a carcinogenic fish borne fluke, requires freshwater snails and fish as intermediate hosts. opisthorchiasis is endemic in parts of southeast asia, including central and south vietnam. in this region the transmission by intermediate hosts has received little attention. therefore, freshwater snails and wild fish from bau my tho, an opisthorchiasis endemic area in binh dinh province were collected for examination of o. viverrini cercariae and metacercariae, respectively. a t ...201728242064
bithynia siamensis goniomphalos, the first intermediate host of opisthorchis viverrini in thailand.opisthorchiasis caused by opisthorchis viverrini (o. viverrini) remains as medically important problem in thailand especially in the north-eastern part. infection with this parasite can lead to cholangiocarcinoma improvement. the highest prevalence of o. viverrini infection has been found in the northeast thailand and is associated with the high incidence rate of cholangiocarcinoma. to complete the life cycle of o. viverrini, the freshwater snails namely bithynia funiculata, bithynia siamensis s ...201526522291
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