Publications

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diversity of begomoviruses associated with mosaic disease of cultivated cassava (manihot esculenta crantz) and its wild relative (manihot glaziovii mull. arg.) in uganda.cassava (manihot esculenta) growing in uganda during 2001-2002 has been screened for the presence of begomoviruses using pcr-rflp, cloning full-length genomic components and nucleotide sequence analysis. in contrast with a recent survey in neighbouring kenya, which identified three distinct strains of east african cassava mosaic virus (eacmv, eacmv-ug and eacmv-ke2) as well as east african cassava mosaic zanzibar virus and the new species east african cassava mosaic kenya virus, only eacmv-ug an ...200818559947
alternate hosts of african cassava mosaic virus and east african cassava mosaic cameroon virus in nigeria.cassava mosaic disease (cmd) caused by african cassava mosaic virus (acmv) and east african cassava mosaic cameroon virus (eacmcv) is the major constraint to cassava production in nigeria. sequences of the dna-a component of acmv and eacmcv isolates from leguminous plant species (senna occidentalis, leucana leucocephala and glycine max), castor oil plant (ricinus communis), a weed host (combretum confertum) and a wild species of cassava (manihot glaziovii) were determined. all acmv isolates from ...200818661095
drought tolerant stem anatomy characteristics in manihot esculenta (euphorbiaceae) and a wild relative.the stem structure of two cassava cultivars, unb 99 and unb 110, known for being adapted to humid conditions and tolerant to drought, respectively, and of a wild species, manihot glaziovii, was examined anatomically. free-hand sections of secondary stems were made, clarified with 50% sodium hypochlorite solution, stained with 1% alcian-blue safranin, and then passed through an ethanol series and butyl acetate, followed by mounting in synthetic resin. m. glaziovii stems had dense prismatic and dr ...201020533197
simultaneous virus-specific detection of the two cassava brown streak-associated viruses by rt-pcr reveals wide distribution in east africa, mixed infections, and infections in manihot glaziovii.the expanding cassava brown streak disease (cbsd) epidemic in east africa is caused by two ipomoviruses (genus ipomovirus; potyviridae), namely, cassava brown streak virus (cbsv), and ugandan cassava brown streak virus (ucbsv) that was described recently. a reverse transcription polymerase chain reaction (rt-pcr) based diagnostic method was developed in this study for simultaneous virus-specific detection of the two viruses. results showed that cbsv and ucbsv are distributed widely in the highla ...201120923689
characterisation and evaluation of a novel feedstock, manihot glaziovii, muell. arg, for production of bioenergy carriers: bioethanol and biogas.the objective of this study was to characterise and evaluate a wild inedible cassava species, manihot glaziovii as feedstock for bioenergy production. tubers obtained from 3 different areas in tanzania were characterised and evaluated for bioethanol and biogas production. these bioenergy carriers were produced both separately and sequentially and their energy values evaluated based on these two approaches. composition analysis demonstrated that m. glaziovii is a suitable feedstock for both bioet ...201425237774
high bioethanol titre from manihot glaziovii through fed-batch simultaneous saccharification and fermentation in automatic gas potential test system.a process for the production of high bioethanol titre was established through fed-batch and simultaneous saccharification and fermentation (fb-ssf) of wild, non-edible cassava manihot glaziovii. fb-ssf allowed fermentation of up to 390g/l of starch-derived glucose achieving high bioethanol concentration of up to 190g/l (24% v/v) with yields of around 94% of the theoretical value. the wild cassava m. glaziovii starch is hydrolysable with a low dosage of amylolytic enzymes (0.1-0.15% v/w, termamyl ...201424534761
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