| mesorhizobium alhagi sp. nov., isolated from wild alhagi sparsifolia in north-western china. | eleven strains that formed symbiotic root nodules on alhagi sparsifolia, designated previously as genospecies ii, were identified as a new lineage of mesorhizobium (alphaproteobacteria) that could be differentiated from all previously recognized species of the genus mesorhizobium by using 16s rrna gene sequences (<97.8 % similarity), dna-dna hybridization (<45 %), dnaj, dnak, reca, glna, nifh, noda and nodc gene sequences, fatty acid profiles (c(18 : 1)omega7c, 35 %;11-methyl c(18 : 1)omega7c, 3 ... | 2010 | 19661514 |
| mesorhizobium camelthorni sp. nov., isolated from alhagi sparsifolia. | nine strains isolated from symbiotic root nodules on alhagi sparsifolia were previously designated as representing genospecies i. phylogenetic analyses indicated that genospecies i was related closely to mesorhizobium alhagi (genospecies ii), and clearly formed a new lineage within the genus mesorhizobium. in this study, we differentiated genospecies i from recognized species of the genus mesorhizobium based on phylogenetic analyses of additional core genes (reca, glna), levels of dna-dna relate ... | 2011 | 20382787 |
| characterization of strains unlike mesorhizobium loti that nodulate lotus spp. in saline soils of granada, spain. | lotus species are forage legumes with potential as pastures in low-fertility and environmentally constrained soils, owing to their high persistence and yield under those conditions. the aim of this work was the characterization of phenetic and genetic diversity of salt-tolerant bacteria able to establish efficient symbiosis with lotus spp. a total of 180 isolates able to nodulate lotus corniculatus and lotus tenuis from two locations in granada, spain, were characterized. molecular identificatio ... | 2010 | 20435777 |
| diverse rhizobia associated with sophora alopecuroides grown in different regions of loess plateau in china. | a total of seventy-five symbiotic bacterial strains isolated from root nodules of wild sophora alopecuroides grown in different regions of china's loess plateau were characterized. based on the combined rflp patterns, thirty-five genotypes were defined among the rhizobia and they were classified into nine genomic species, including mesorhizobium alhagi and m. gobiense as the main groups, as well as agrobacterium tumefaciens, m. amorphae, phyllobacterium trifolii, rhizobium giardinii, r. indigofe ... | 2010 | 20965680 |
| draft genome sequence of mesorhizobium alhagi ccnwxj12-2t, a novel salt-resistant species isolated from the desert of northwestern china. | mesorhizobium alhagi strain ccnwxj12-2(t) is a novel species of soil-dwelling, nitrogen-fixing bacteria that can form symbiotic root nodules with alhagi sparsifolia. moreover, the strain has high resistance to salt and alkali. here we report the draft genome sequence of mesorhizobium alhagi strain ccnwxj12-2(t). a large number of osmotic regulation-related genes have been identified. | 2012 | 22328758 |
| global transcriptome analysis of mesorhizobium alhagi ccnwxj12-2 under salt stress. | mesorhizobium alhagi ccnwxj12-2 is a α-proteobacterium which could be able to fix nitrogen in the nodules formed with alhagi sparsifolia in northwest of china. desiccation and high salinity are the two major environmental problems faced by m. alhagi ccnwxj12-2. in order to identify genes involved in salt-stress adaption, a global transcriptional analysis of m. alhagi ccnwxj12-2 growing under salt-free and high salt conditions was carried out. the next generation sequencing technology, rna-seq, w ... | 2014 | 25539655 |
| functional analysis of prka - a putative serine protein kinase from mesorhizobium alhagi ccnwxj12-2 - in stress resistance. | serine/threonine protein kinases are highly conserved kinases with a wide distribution in microbes and with multiple functions. mesorhizobium alhagi ccnwxj12-2, a α-proteobacterium which could be able to form symbiosis with alhagi sparsifolia in northwest of china, contains a putative prka-family serine protein kinase, prka. in our previous study, the expression of prka was found to be downregulated in high-salt conditions. to elucidate the function of m. alhagi prka, a prka deletion mutant was ... | 2016 | 27686068 |
| the metabolism of neonicotinoid insecticide thiamethoxam by soil enrichment cultures, and the bacterial diversity and plant growth-promoting properties of the cultured isolates. | a soil enrichment culture (sec) rapidly degraded 96% of 200 mg l(-1) neonicotinoid insecticide thiamethoxam (tmx) in msm broth within 30 d; therefore, its metabolic pathway of tmx, bacterial diversity and plant growth-promoting rhizobacteria (pgpr) activities of the cultured isolates were studied. the sec transformed tmx via the nitro reduction pathway to form nitrso, urea metabolites and via cleavage of the oxadiazine cycle to form a new metabolite, hydroxyl clo-tri. in addition, 16s rrna gene- ... | 2014 | 24762175 |
| role of exopolysaccharide in salt stress resistance and cell motility of mesorhizobium alhagi ccnwxj12-2(t). | mesorhizobium alhagi, a legume-symbiont soil bacterium that forms nodules with the desert plant alhagi sparsifolia, can produce large amounts of exopolysaccharide (eps) using mannitol as carbon source. however, the role of eps in m. alhagi ccnwxj12-2(t), an eps-producing rhizobium with high salt resistance, remains uncharacterized. here, we studied the role of eps in m. alhagi ccnwxj12-2(t) using eps-deficient mutants constructed by transposon mutagenesis. the insertion sites of six eps-deficien ... | 2017 | 28097405 |
| evolution and taxonomy of native mesorhizobia nodulating medicinal glycyrrhiza species in china. | previously, 159 bacterial strains were isolated from the root nodules of wild perennial glycyrrhiza legume species grown on 40 sites in central and north-western china, in which 57 strains were classified as "true symbionts" belonging to the genus mesorhizobium based on amplified fragment length polymorphism (aflp) genomic fingerprinting and partial sequences of the 16s rrna gene [20]. in the present work, the phylogeny of glycyrrhiza nodulating mesorhizobia was further examined by multilocus se ... | 2016 | 27105685 |