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vitex agnus-castus is a preferred host plant for hyalesthes obsoletus.hyalesthes obsoletus signoret (homoptera: cixiidae) is a polyphagous planthopper that transmits stolbur phytoplasma (a causative agent of "yellows" disease) to various weeds, members of the solanaceae, and wine grapes (vitis vinifera l.) in europe and the middle east. planthoppers were collected by hand vacuuming eight native plant species. vitex agnus-castus l., a shrub in the verbenaceae, hosted the largest number of h. obsoletus, although olea europaea l. also served as a host for adults. usi ...200516124232
characterization of bois noir isolates by restriction fragment length polymorphism of a stolbur-specific putative membrane protein gene.bois noir phytoplasma (bnp), widespread in wine-producing areas of europe and endemic in france and italy, is classified in the 16srxii-a subgroup, whose members are referred to as stolbur phytoplasmas. the 16s rdna gene of stolbur phytoplasma shows low variability, and few non-ribosomal genes are available as markers to assess variation among isolates. we used the stolbur-specific stol-1h10 gene, encoding a putative membrane-exposed protein, to investigate genetic diversity of french and italia ...200919453230
the sensory structures of the antennal flagellum in hyalesthes obsoletus (hemiptera: fulgoromorpha: cixiidae): a functional reduction?despite their relevance as harmful pests on plants of economic importance, hemiptera fulgoromorpha have been poorly studied as regards their antennal sensory structures. in particular, the flagellum has been neglected and, therefore, to date there are no data on its structural organization and sensory equipment. in order to fill this gap, we carried out a study on the sensillum types and distribution on the flagellum of the planthopper hyalesthes obsoletus signoret, an efficient vector of the st ...200919682602
bacterial endosymbiont localization in hyalesthes obsoletus, the insect vector of bois noir in vitis vinifera.one emerging disease of grapevine in europe is bois noir (bn), a phytoplasmosis caused by "candidatus phytoplasma solani" and spread in vineyards by the planthopper hyalesthes obsoletus (hemiptera: cixiidae). here we present the first full characterization of the bacterial community of this important disease vector collected from bn-contaminated areas in piedmont, italy. length heterogeneity pcr and denaturing gradient gel electrophoresis analysis targeting the 16s rrna gene revealed the presenc ...201021183640
impact of vector dispersal and host-plant fidelity on the dissemination of an emerging plant pathogen.dissemination of vector-transmitted pathogens depend on the survival and dispersal of the vector and the vector's ability to transmit the pathogen, while the host range of vector and pathogen determine the breath of transmission possibilities. in this study, we address how the interaction between dispersal and plant fidelities of a pathogen (stolbur phytoplasma tuf-a) and its vector (hyalesthes obsoletus: cixiidae) affect the emergence of the pathogen. using genetic markers, we analysed the geog ...201223284774
an abundant 'candidatus phytoplasma solani' tuf b strain is associated with grapevine, stinging nettle and hyalesthes obsoletus.bois noir (bn) associated with 'candidatus phytoplasma solani' (stolbur) is regularly found in austrian vine growing regions. investigations between 2003 and 2008 indicated sporadic presence of the confirmed disease vector hyalesthes obsoletus and frequent infections of bindweed and grapevine. infections of nettles were rare. in contrast present investigations revealed a mass occurrence of h. obsoletus almost exclusively on stinging nettle. the high population densities of h. obsoletus on urtica ...201425309042
genetic variability of stolbur phytoplasma in hyalesthes obsoletus (hemiptera: cixiidae) and its main host plants in vineyard agroecosystems.bois noir is an economically important grapevine yellows that is induced by 'candidatus phytoplasma solani' and principally vectored by the planthopper hyalesthes obsoletus signoret (hemiptera: cixiidae). this study explores the 'ca. p. solani' genetic variability associated to the nettle-h. obsoletus and bindweed-h. obsoletus systems in vineyard agroecosystems of the central-eastern italy. molecular characterization of 'ca. p. solani' isolates was carried out using polymerase chain reaction/res ...201526470289
identification and phenology of hyalesthes obsoletus (hemiptera: auchenorrhyncha: cixiidae) nymphal instars.urtica dioica and convolvulus arvensis are the main host plants of hyalesthes obsoletus and play an important role in the epidemiology of bois noir of grapevines. the earliest survey, which was carried out to compare the phenology of nymphal instars on u. dioica and c. arvensis, had highlighted some problems in the identification of the instars. therefore, the correct identification of nymphs to species and instar level became a preliminary aim of this research. adults and nymphs attributable to ...201222360966
bacterial associates of hyalesthes obsoletus (hemiptera: cixiidae), the insect vector of bois noir disease, with a focus on cultivable bacteria.the planthopper hyalesthes obsoletus (hemiptera: cixiidae) is an important vector of phytoplasma diseases in grapevine. in the current study, the bacterial community compositions of symbionts of this insect were examined. two dominant bacterial lineages were identified by mass sequencing: the obligate symbiont candidatus sulcia, and a facultative symbiont that is closely related to pectobacterium sp. and to bev, a cultivable symbiont of another phytoplasma vector, the leafhopper euscelidius vari ...201727602526
source of hyalesthes obsoletus signoret planthopper (hemiptera: cixiidae) in southern france and potential effects of landscape.cixiid planthoppers are considered of major economic importance, as they can transmit phytoplasmas responsible for many plant diseases. while thorougly studied in vineyards, the epidemiology of stolbur phytoplasma, transmitted by hyalesthes obsoletus signoret, was rarely investigated on minor crops as lavender, where it leads to 'yellow decline' disease and large economic losses. the objective of this paper is to understand the effect of the local landscape characteristics on the presence and de ...201728891460
survival relative to new and ancestral host plants, phytoplasma infection, and genetic constitution in host races of a polyphagous insect disease vector.dissemination of vectorborne diseases depends strongly on the vector's host range and the pathogen's reservoir range. because vectors interact with pathogens, the direction and strength of a vector's host shift is vital for understanding epidemiology and is embedded in the framework of ecological specialization. this study investigates survival in host-race evolution of a polyphagous insect disease vector, hyalesthes obsoletus, whether survival is related to the direction of the host shift (from ...201425247065
natural phytoplasma infection of four phloem-feeding auchenorrhyncha across vineyard agroecosystems in central-eastern italy.the seasonal variations of grapevine yellow phytoplasma were investigated in four phloem-feeding planthopper and leafhopper species that are vectors of plant disease agents. in total, 1,148 wild specimens were collected from three vineyard agroecosystems in the marche region (central-eastern italy), from may to september 2008, and analyzed using polymerase chain reaction-restriction fragment-length polymorphism methods. of 525 euscelis lineolatus brullé, 25.1% were positive for aster yellow phyt ...201323786045
sympatric diversification vs. immigration: deciphering host-plant specialization in a polyphagous insect, the stolbur phytoplasma vector hyalesthes obsoletus (cixiidae).the epidemiology of vector transmitted plant diseases is highly influenced by dispersal and the host-plant range of the vector. widening the vector's host range may increase transmission potential, whereas specialization may induce specific disease cycles. the process leading to a vector's host shift and its epidemiological outcome is therefore embedded in the frameworks of sympatric evolution vs. immigration of preadapted populations. in this study, we analyse whether a host shift of the stolbu ...201323452165
draft genome sequence of a dyella-like bacterium from the planthopper hyalesthes obsoletus.we report here the draft genome sequence of a dyella-like bacterium (dlb) isolated from hyalesthes obsoletus, the insect vector of the uncultivable mollicute bacterium "candidatus phytoplasma." this isolate inhibits spiroplasma melliferum, a cultivable mollicute. the draft genome of dlb consists of 4,196,214 bp, with a 68.6% g+c content, and 3,757 genes were predicted.201627445378
[transmission of stolbur virus of pepper and aubergines by grafting and the leaf-hopper hyalesthes obsoletus sign]. 20005619957
introduction of a putative biocontrol agent into a range of phytoplasma- and liberibacter-susceptible crop plants.phytoplasma, the causative agent of bois noir disease of grapevines, are vectored by the planthopper hyalesthes obsoletus (hemiptera: cixiidae). a dyella-like bacterium (dlb) isolated from h. obsoletus inhibits the growth of spiroplasma melliferum, a cultivable relative of phytoplasma. additional evidence suggests that dlb can reduce the symptoms of yellows disease in grapevine plantlets. the aim of this study was to test whether dlb could colonize a range of phytoplasma- and liberibacter-sensit ...201729072824
dyella-like bacterium isolated from an insect as a potential biocontrol agent against grapevine yellows.yellows diseases, caused by phytopathogenic bacteria of the genus phytoplasma, are a major threat to grapevines worldwide. because conventional applications against this pathogen are inefficient, and disease management is highly challenging, the use of beneficial bacteria has been suggested as a biocontrol solution. a dyella-like bacterium (dlb), isolated from the israeli insect vector of grapevine yellows hyalesthes obsoletus, was suggested to be an endophyte. to test this hypothesis, the bacte ...201728990480
molecular diversity of phytoplasmas associated with grapevine yellows disease in north-eastern italy.a 3-year survey was conducted in northern italy to verify the presence and diversity of phytoplasmas in selected vineyards showing symptoms of severe yellows. symptomatic and asymptomatic grapevines were sampled, and insects were collected using yellow sticky traps. the phytoplasmas detected in grapevine samples were different according to the years: "flavescence dorée" (16srv-c/d) was detected together with other phytoplasmas such as 16srxii-a ('candidatus phytoplasma solani'-related, bois noir ...201828945521
vineyard colonization by hyalesthes obsoletus (hemiptera: cixiidae) induced by stinging nettle cut along surrounding ditches.stinging nettle (urtica dioica l.) is the most important host plant for both phytoplasma associated with bois noir disease of the grapevine and its vector hyalesthes obsoletus signoret (hemiptera: cixiidae). vector abundance in vineyards is favored by stinging nettle growing in surrounding areas. nettle control by herbicides or cutting can reduce vector population in vineyards. however, chemical weeding can cause environmental problems. many authors suggest that stinging nettle control applied d ...201626352752
secondary spectral components of substrate-borne vibrational signals affect male preference.animal sexual signals contain information about both compatibility and quality of the signaller, but combined with influence of the signalling medium, the complexity of mate selection makes it difficult to separate different components of this process. we approached the problem of teasing apart different functions of sexual signals by using the planthopper hyalesthes obsoletus, which uses unimodal vibrational communication, as a model. vibrational signals are known to encode information about id ...201525735461
sex-dispersal differences of four phloem-feeding vectors and their relationship to wild-plant abundance in vineyard agroecosystems.sex-dispersal differences of four phloem-feeding vectors and their relationship to wild-plant abundance were analyzed over 2 yr in two vineyard agroecosystems. adults of euscelis lineolatus brullè, neoaliturus fenestratus (herrich-schäffer), psammotettix alienus (dahlbom), and hyalesthes obsoletus signoret were sampled by using yellow sticky traps at two heights (20 and 150 cm) above the ground in a regular geo-referenced grid. wild vegetation was surveyed around each sampling point. the most ab ...201324498727
comparative neuroanatomy of the antennal lobes of 2 homopteran species.we compared the morphology of the primary olfactory center, the antennal lobe (al), in 2 homopteran insects, hyalesthes obsoletus signoret (homoptera: cixiidae) and scaphoideus titanus ball (homoptera: cicadomorpha). the comparison between the als of the 2 species is particularly interesting considering that, although both use volatile cues to locate their host plants, their feeding behavior differs considerably: specifically, h. obsoletus is a highly polyphagous species, whereas s. titanus is s ...201424443423
influence of environment and climate on occurrence of the cixiid planthopper hyalesthes obsoletus, the vector of the grapevine disease 'bois noir'.species distribution models (sdms), which are well established in many fields of biological research, are still uncommon in the agricultural risk analysis of pest insects. to exemplify the use of sdms, we investigated the influence of environmental factors on the occurrence of hyalesthes obsoletus signoret (hemiptera: cixiidae). the planthopper is the only known vector of the grapevine yellows disease 'bois noir'. the study was conducted in 145 locations in the baden region of southwest germany. ...201323628148
the johnston's organ of three homopteran species: a comparative ultrastructural study.a transmission electron-microscopy study has been carried out on the pedicel of three homopteran species, with particular focus on the leafhopper scaphoideus titanus ball. the two other species, the planthoppers hyalesthes obsoletus signoret and metcalfa pruinosa say, were investigated in order to compare the ultrastructure of the johnston's organ (jo) among representatives of the auchenorrhyncha group. the results showed the presence of a well developed jo located within the pedicel. depending ...201323428838
perception of host plant volatiles in hyalesthes obsoletus: behavior, morphology, and electrophysiology.the palearctic planthopper hyalesthes obsoletus is the natural vector of the grapevine yellow disease bois noir. grapevine is an occasional host plant of this polyphagous planthopper. to deepen our knowledge of the role of plant volatile organic compounds for h. obsoletus host plant searching, we carried out behavioral, morphological, and electrophysiological studies. we tested the attraction of h. obsoletus to nettle, field bindweed, hedge bindweed, chaste tree, and grapevine by using a y-shape ...201222730108
[data on tomato blight in the crimea; biological data on the vector hyalesthes obsoletus]. 200614785559
'candidatus phytoplasma solani', a novel taxon associated with stolbur- and bois noir-related diseases of plants.phytoplasmas classified in group 16srxii infect a wide range of plants and are transmitted by polyphagous planthoppers of the family cixiidae. based on 16s rrna gene sequence identity and biological properties, group 16srxii encompasses several species, including 'candidatus phytoplasma australiense', 'candidatus phytoplasma japonicum' and 'candidatus phytoplasma fragariae'. other group 16srxii phytoplasma strains are associated with stolbur disease in wild and cultivated herbaceous and woody pl ...201323334879
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