| ultrastructure of the invasion of eimeria magna sporozoites into cultured cells. | monolayers of bovine kidney cells were overlaid with eimeria magna sporozoites and observed with phase-contrast optics until penetration of the cells by the parasites had begun. cells and penetrating parasites were fixed with glutaraldehyde and oso4-containing ruthenium red, dehydrated, and embedded in situ. cells being penetrated were selected for study in the electron microscope. the lack of intracellular staining with ruthenium red and intact plasmalemmas of cells being penetrated, was acc ... | 1975 | 51083 |
| further studies on the development of gamonts and oocysts of eimeria magna in cultured cells. | monolayer, cell-line cultures of embryonic bovine trachea, madin-darby bovine kidney (mdbk), and monolayers (rk-1) or aggregates of primary rabbit kidney cells were inoculated with merozoites obtained from rabbits that had been inoculated 3 to 5 1/2 days earlier with eimeria magna. merozoites obtained from from rabbits 3 days entered cells and underwent only merogony, whereas 3 1/2-5 1/2-day-old merozoites formed gamonts as well as meronts. merozoites arising from the first or second meront gene ... | 1979 | 574539 |
| life cycle studies with eimeria magna pérard, 1925. | excystation of eimeria magna required exposure to co2 at body temperature followed by trypsin and bile. incubation in the presence of co2 produced marked effects on the inner layer of the oocyst wall. the evidence suggests that co2 stimulates enzymic activity from within the oocyst rather than exerting a physico-chemical effect on the oocyst wall from the outside. released sporozoites measured 19.65 x 3.33 mum. in the rabbit, e. magna undergoes at least 5 cycles of asexual schizogony before game ... | 1976 | 997720 |
| possible secretory function of the rhoptries of eimeria magna during penetration of cultured cells. | ultrastructure of the penetration of eimeria magna sporozoites into embryonic bovine trachea cells demonstrated that the host cell membrane was not broken during entry of the parasite. this membrane did, however, undergo alterations characterized by blebbing of vesicles, thickening, and eventual disorganization once penetration was completed. concurrent with the entrance of the parasite into the cell, and the subsequent membrane alterations, was the appearance of empty membrane saccules, probabl ... | 1976 | 1003289 |
| effects of antiphagocytic agents on penetration of eimeria magna sporozoites into cultured cells. | madin-darby bovine kidney cells were treated with sodium flouride, iodoacetate, and 2-deosyglucose, reagents that block glycolysis, and thus reduce phagocytosis. sporozoites readily entered cells whose atp stores were largely depleted. they also entered cells treated with colchicine, colcemid, and vinblastine. these latter agents did not inhibit sporozite motility after 6 hr incubation. cytochalasin b prevented penetration of cells by inhibiting the motility of sporozoites. this effect was rever ... | 1976 | 1263027 |
| fine-structural aspects of microgametogenesis of eimeria magna in rabbits and in kidney cell cultures. | the fine-structural aspects of development of microgamonts of eimeria magna were studied in kidney cell cultures and in experimentally infected rabbits. spheroidal masses of gamont-like cytoplasm containing ribosomes, polyribosomes, and amylopectin granules were found within the parasitophorus vacuole; these bodies were apparently pinched off the surface of the gamont. nucleoli were present in the early stages of nuclear division but disappeared as development proceeded. | 1976 | 1271303 |
| ultrastructural changes following treatment with a microwave pulse in the oocyst of eimeria magna perard, 1925. | the ultrastructure of oocysts of eimeria magna was studied before and after their exposure to unique pulse of microwaves (2.450 mhz; 600 w) of different durations (10, 15 and 20 s). following treatment, the progressive destruction of the three layers of the oocyst wall was observed, the innermost being destroyed first. internal structures were also affected, resulting in swollen mitochondria, a loss of ribosomes and fragmentation of the rough endoplasmic reticulum; moreover, the wall-forming bod ... | 1991 | 1792228 |
| efficacy of diclazuril in the control of intestinal coccidiosis in rabbits. | the anticoccidial efficacy of diclazuril was studied in rabbits artificially infected with eimeria flavescens, eimeria intestinalis, eimeria magna and eimeria perforans. continuous administration at 1 and 2 ppm in pelleted feed proved to be highly efficacious in controlling oocyst output and faecal scores. the weight gain was comparable and the feed efficiency slightly improved compared with the non-infected, non-medicated controls, and clinical signs were fully prevented. medication of rabbits ... | 1989 | 2617823 |
| efficacy of diclazuril against robenidine resistant eimeria magna in rabbits. | | 1989 | 2773198 |
| efficacy of diclazuril in the prevention and cure of intestinal and hepatic coccidiosis in rabbits. | the efficacy of diclazuril against intestinal and hepatic coccidiosis was studied in artificially infected rabbits. prophylaxis against intestinal coccidiosis was evaluated using a mixed infection of eimeria intestinalis, eimeria magna and eimeria perforans. continuous medication in the feed at 1 p.p.m. was 100% effective in reducing oocyst output and faecal scores, and weight gain and feed efficiency were normal. hepatic coccidiosis induced by eimeria stiedai was prevented at 0.5 and 1 p.p.m. a ... | 1989 | 2773264 |
| identification of virus-like particles in eimeria stiedae. | when nucleic acid samples purified from sporozoites of eimeria stiedae were analyzed by agarose gel electrophoresis, an ethidium-stainable band with an apparent electrophoretic mobility of 6.5 kb was consistently observed. the band was readily degradable upon rnase treatment, and its susceptibility towards ribonuclease a on a decreasing ionic strength was suggestive of double-stranded rna (dsrna). electron microscopy revealed spherical, probably icosahedral, virus-like particles (vlp) with a dia ... | 1989 | 2797059 |
| evolution of coccidial infection in commercial and domestic rabbits between 1982 and 1986. | caecal samples were collected from 751 domestic rabbits of various origin and from 1229 diarrhoeic rabbits issued from 61 commercial rabbitries. they were screened for coccidiosis. in 1982, the year of introduction of the anticoccidial robenidine in commercial rabbit feeds, a dramatic decrease of coccidial infection ratio was detected in commercial rabbitries: only 6% of samples contained greater than 100 oocysts per gram against 85% in 1979, when sulphaquinoxaline/pyrimethamine was used. only e ... | 1988 | 3201707 |
| eimeria magna: resistance against robenidine in the rabbit. | | 1987 | 3445427 |
| [etiopathogenesis and epizootiology of coccidiosis in rabbits]. | studies were carried out on the etiology and epizootiology of rabbit coccidiosis. three experimental groups of rabbits were infected with intestinal, liver, and mixed intestinal and liver coccidia. the shedding of oocysts and the clinical course of the disease were followed up, with description of the morphologic changes and the results of the biochemical examinations of the blood. a total of eight species of coccidia were established in all cases--eimeria magna, e. intestinalis, e. piriformis, ... | 1986 | 3811201 |
| stages of merogony in multinucleate merozoites of eimeria magna pérard, 1925. | | 1972 | 4342298 |
| fine-structural aspects of macrogametogenesis in eimeria magna. | | 1973 | 4350904 |
| the ultrastructural cytology of the nucleolus of eimeria magna gamonts. | | 1972 | 4560437 |
| further asexual development of eimeria magna merozoites in cell cultures. | | 1973 | 4578978 |
| motility of macrogamonts of eimeria magna (coccidia) in cell culture. | amoeboid movement of spheroid or ovoid immature or mature macrogamonts within cultured madin-darby bovine cells usually began with the formation of a pseudopodium-like protrusion at the margin of the gamont. the protrusion enlarged as the gamont cytoplasm and nucleus moved into the protruded area. during movement, macrogamonts had an elongate shape. the body of the gamont was constricted as it moved through the cell cytoplasm or from one cell to an adjacent one. after movement had ceased, the ga ... | 1972 | 4673342 |
| development of first- and second-generation schizonts of eimeria magna from rabbits in cell cultures. | | 1971 | 4943567 |
| [ultrastructural study of merozoites and schizogony of the coccidia (eimeriina): eimeria magna (perard 1925) from the intestine of rabbits and e. tenella (railliet and lucet, 1891) from the cecums of chickens]. | | 1969 | 5806191 |
| acquired resistance to reinfection of rabbits with eimeria magna. | | 1967 | 6063583 |
| intestinal motor and transit disturbances associated with experimental coccidiosis (eimeria magna) in the rabbit. | digestive motility has been analyzed in three rabbits chronically fitted with intraparietal electrodes, during 6 days before and 18 days after infection with 100,000 oocysts of e. magna. the rate of flow and the transit time of digesta in the small intestine were determined during the same period by marker dilution techniques in three other rabbits fitted with an intraluminal catheter and a cannula at respectively 1.2 and 0.4 m from the ileo-caecal valve. the main disturbances of small intestine ... | 1982 | 7137853 |
| morphological factors influencing transepithelial conductance in a rabbit model of ileitis. | infection of rabbits with coccidia (eimeria magna) causes chronic ileal inflammation and diarrhea. inflamed ileum also shows decreased transmural conductance. the aim of this study was to characterize morphological factors known to affect paracellular permeability that may alter transmural conductance in inflamed ileum. | 1995 | 7797012 |
| eimeria magna: pathogenicity, immunogenicity and selection of a precocious line. | a precocious line (premag) of eimeria magna in rabbits was obtained by selecting for early development of oocysts. the prepatent period was shortened by 46 h. the pathogenicity of premag was substantially reduced and its reproductive potential was much lower (500 times) than that of the parent strain. rabbits given 2500 oocysts of premag were almost totally protected against a challenge with the parent strain. as in other precocious lines of coccidia from the rabbit, premag showed morphological ... | 1995 | 8644456 |
| eimeria magna pérard, 1925: study of the endogenous development of parental and precocious strains. | the endogenous development of a parental strain of e. magna and its deriving precocious line was studied after inoculation of coccidia-free rabbits with oocysts or sporocysts directly into the duodenum and using electron microscopy. four meront generations could be observed mainly in the jejunum and ileum for the parent strain. the first merogony began 24 h post-inoculation (h p.i.). and meronts were matured about 48 h p.i. the second and the third generation were complete at 66 and 84 h p.i. re ... | 1996 | 8983147 |
| vaccination against eimeria magna coccidiosis using spray dispersion of precocious line oocysts in the nest box. | coccidiosis mostly affects young rabbits just after weaning (5- to 6-week-old animals). prevention of this disease must therefore be initiated before weaning. 'precocious lines', derived from field species display good immunogenicity, though not pathogenic when administered at the right dose. in the present work, we tested the vaccination method of the whole litter at 25 days of age by spray dispersion of oocysts of a precocious line of eimeria magna in the nest box. three doses were tested, d1 ... | 1997 | 9195710 |
| acquired protection of the rabbit (oryctolagus cuniculus) against coccidiosis using a precocious line of eimeria magna: effect of vaccine dose and age at vaccination. | sucklings were vaccinated orally once at 25, 27 or 29 days of age with a precocious line of eimeria magna. each group received two doses varying from 3.5 x 10(2) to 3.5 x 10(4) oocysts. at 36 days of age, animals received a challenge inoculation with 10(4) oocysts of the wild strain of e.magna. vaccination reduced oocyst output 10 to 1000 times after the challenge inoculation and prevented the decrease in the weight gain observed in non vaccinated challenged animals. when the vaccination was per ... | 1997 | 9195729 |
| rabbit chronic ileitis leads to up-regulation of adenosine a1/a3 gene products, oxidative stress, and immune modulation. | a rabbit model of chronic ileitis has helped decipher the mechanism of alteration of multiple electrolyte and nutrient malabsorptions in inflammatory bowel disease (ibd). this study examined alterations in the adenosine a1/a3 receptor, oxidant, antioxidant, and immune-inflammatory pathways in chronic ileitis. chronic ileal inflammation was induced 13-15 days after infection with 10,000 eimeria magna oocytes. quantitative analysis in 16 rabbits was done for oxidants, antioxidants, a1 and a3 trans ... | 2003 | 12732366 |
| effect of subcutaneously administered diclazuril on the output of eimeria species oocysts by experimentally infected rabbits. | the effect of subcutaneously injected diclazuril on the output of eimeria species oocysts was studied in experimentally infected rabbits. diclazuril was administered either prophylactically at 0.5, 1 or 2 mg/kg bodyweight two days before each rabbit was inoculated with 20,000 oocysts of a mixed-species field isolate of eimeria or therapeutically at 1, 2 or 4 mg/kg bodyweight five days after they were inoculated. the prophylactic treatments significantly reduced (p<0.05) the output of oocysts in ... | 2008 | 18245747 |
| fecal occult blood manifestation of intestinal eimeria spp. infection in rabbit. | the relationship between oocysts production and fecal occult blood manifestation was studied in rabbits experimentally infected with a single species of eimeria. pure single eimeria species infection in non-infected rabbits for 6 species of eimeria, namely: eimeria media, eimeria magna, eimeria perforans, eimeria coecicola, eimeria piriformis, and eimeria exigua were produced using a single oocyst. fecal samples from these single eimeria species infected rabbits were collected daily from day 1 t ... | 2009 | 19233566 |
| comparison of toltrazuril and sulphadimethoxine in the treatment of intestinal coccidiosis in pet rabbits. | treatment of intestinal coccidiosis was studied in domestic pet rabbits. in 45 rabbits aged four months or more, coccidial oocysts were observed in the faeces of 35 rabbits at a mean density of 806 opg (range 50 to 6800 opg). eimeria magna was the dominant species, with eimeria media and eimeria intestinalis also being common. the presence of the hepatic species eimeria stiedae was not recorded. a single oral dose of 2.5 mg/kg or 5.0 mg/kg toltrazuril, or a single oral dose of 50 mg/kg sulphadim ... | 2010 | 20729515 |
| prevalence, infectivity and oocyst sporulation time of rabbit-coccidia in taiwan. | prevalence of eimeria species parasitizing rabbits (oryctolagus cuniculus) in taiwan were investigated. six eimeria species, namely: eimeria media (prevalence, 158/642; 24.6%), eimeria magna (101/642; 15.7%), eimeria perforans (58/642; 9.0%), eimeria coecicola (46/642; 7.2%), eimeria piriformis (16/642; 2.5%), and eimeria exigua (9/642; 1.4%) were observed. the overall prevalence of these coccidial infections in rabbits from pet shops and farms was 46.2% and 41.7%, respectively. concurrent infec ... | 2010 | 21399582 |
| eimeria species in wild rabbits (oryctolagus cuniculus) in fars province, iran. | this investigation was accomplished during february to november 2008. a total of 71 wild rabbits (oryctolagus cuniculus) of about 5 to 12 months age were collected alive from different parts of fars province, south of iran. faecal sampling was carried out directly from recti and the oocysts were isolated using sedimentation and floatation techniques and the sporulated oocyst were identified based on morphological and biological characteristics. all the rabbits were apparently healthy and showed ... | 2010 | 21399588 |
| vaccination of rabbits against coccidiosis using precocious lines of eimeria magna and eimeria media in benin. | three groups of twelve 35-day-old rabbits were used for the experiment. two groups were vaccinated with a mixture of precocious lines of eimeria magna and eimeria media originating from corresponding wild strains isolated in benin. one group benefited of a booster whereas the second one was kept without booster. a third non-vaccinated group was used as control. all groups were challenged per os with an equal mixture of the wild strains of e. magna and e. media at a dose of 104 oocysts per animal ... | 2011 | 21890276 |
| transgenic eimeria magna pérard, 1925 displays similar parasitological properties to the wild-type strain and induces an exogenous protein-specific immune response in rabbits (oryctolagus cuniculus l.). | rabbit coccidiosis causes great economic losses to world rabbitries. little work has been done considering genetic manipulation on the etiological agents, rabbit eimeria spp. in this study, we constructed a transgenic line of eimeria magna (emager) expressing enhanced yellow fluorescent protein (eyfp) and red fluorescent protein (rfp) using regulatory sequences of eimeria tenella and toxoplasma gondii. we observed the life cycle of emager and confirmed that the transgenic parasites express exoge ... | 2017 | 28167939 |
| high pathogenicity and strong immunogenicity of a chinese isolate of eimeria magna pérard, 1925. | coccidia infection of rabbits with one or several species of parasites of the genus eimeria causes coccidiosis, a disease leading to huge economic losses in the rabbit industry. eimeria magna, one of the causal agents of rabbit coccidiosis, was characterized as mildly pathogenic and moderately immunogenic in previous studies. in this study, we identified a chinese isolate of e. magna by testing its biological features (oocyst morphology and size, prepatent time) and sequencing its internal trans ... | 2017 | 28132801 |
| mast cell regulation of na-glutamine co-transporters b0at1 in villus and sn2 in crypt cells during chronic intestinal inflammation. | in the chronically inflamed rabbit small intestine, brush border membrane (bbm) na-glutamine co-transport is inhibited in villus cells (mediated by b0at1), while it is stimulated in crypt cells (mediated by sn2/snat5). how mast cells, known to be enhanced in the chronically inflamed intestine, may regulate b0at1 in villus and sn2/snat5 in crypt cell is unknown. thus, the aim of the present study is to determine the regulation of b0at1 and sn2/snat5 by mast cells during chronic enteritis. | 2015 | 25884559 |
| the complete mitochondrial genome sequence of eimeria magna (apicomplexa: coccidia). | in the present study, we determined the complete mitochondrial dna (mtdna) sequence of eimeria magna from rabbits for the first time, and compared its gene contents and genome organizations with that of seven eimeria spp. from domestic chickens. the size of the complete mt genome sequence of e. magna is 6249 bp, which consists of 3 protein-coding genes (cytb, cox1 and cox3), 12 gene fragments for the large subunit (lsu) rrna, and 7 gene fragments for the small subunit (ssu) rrna, without transfe ... | 2015 | 24328820 |
| unique regulation of na-glutamine cotransporter sn2/snat5 in rabbit intestinal crypt cells during chronic enteritis. | the only na-nutrient cotransporter described in mammalian small intestinal crypt cells is sn2/snat5, which facilitates glutamine uptake. in a rabbit model of chronic intestinal inflammation, sn2 stimulation is secondary to an increase in affinity of the cotransporter for glutamine. however, the immune regulation of sn2 in the crypt cells during chronic intestinal inflammation is unknown. we sought to determine the mechanism of regulation of na-nutrient cotransporter sn2 by arachidonic acid metab ... | 2017 | 29271063 |