| tomato chocolàte virus: a new plant virus infecting tomato and a proposed member of the genus torradovirus. | a new virus was isolated from a tomato plant from guatemala showing necrotic spots on the bases of the leaves and chocolate-brown patches on the fruits. structural and molecular analysis showed the virus to be clearly related to but distinct from the recently described tomato torrado virus (totv) and tomato marchitez virus (tomarv), both members of the genus torradovirus. the name tomato chocolàte virus is proposed for this new torradovirus. | 2010 | 20229114 |
| aphid transmission of lettuce necrotic leaf curl virus, a member of a tentative new subgroup within the genus torradovirus. | lettuce necrotic leaf curl virus (lnlcv) was described as the first non-tomato-infecting member of the genus torradovirus. until today, the virus was found only in the netherlands in two different areas in open field crops of lettuce. in 2015, lnlcv was accepted by the ictv as a new member of the genus torradovirus. the tomato-infecting (ti) torradoviruses tomato torrado virus (totv), tomato marchitez virus (tomarv) and tomato chocolàte virus (tochv) are transmitted by at least three whitefly sp ... | 2017 | 28223184 |
| torradoviruses are transmitted in a semi-persistent and stylet-borne manner by three whitefly vectors. | members of the genus torradovirus (family secoviridae, type species tomato torrado virus, totv) are spherical plant viruses transmitted by the whitefly species trialeurodes vaporariorum and bemisia tabaci. knowledge on the mode of vector transmission is lacking for torradoviruses. here, the mode of transmission was determined for tomato marchitez virus (tomarv). a minimal acquisition access period (aap) and inoculation access period (iap) of approximately 2h each was required for its transmissio ... | 2014 | 24342139 |
| two generic pcr primer sets for the detection of members of the genus torradovirus. | two degenerate primer pairs were designed for the universal detection of members of the genus torradovirus. primer pair torrado-1f/torrado-1r was designed based on the rna-dependent rna polymerase region located in rna1 and primer pair torrado-2f/torrado-2r based on a region overlapping the two first coat proteins vp35 and vp26 in rna2. the primers were used in two-step and one-step rt-pcr protocols. both primer pairs were able to detect 14 out of 15 isolates belonging to the two torradovirus sp ... | 2012 | 22771385 |
| identification of the cleavage sites of the rna2-encoded polyproteins for two members of the genus torradovirus by n-terminal sequencing of the virion capsid proteins. | torradoviruses, family secoviridae, are emergent bipartite rna plant viruses. rna1 is ca. 7kb and has one open reading frame (orf) encoding for the protease, helicase and rna-dependent rna polymerase (rdrp). rna2 is ca. 5kb and has two orfs. rna2-orf1 encodes for a putative protein with unknown function(s). rna2-orf2 encodes for a putative movement protein and three capsid proteins. little is known about the replication and polyprotein processing strategies of torradoviruses. here, the cleavage ... | 2016 | 27567259 |
| rna1-independent replication and gfp expression from tomato marchitez virus isolate m cloned cdna. | tomato marchitez virus (tomarv; synonymous with tomato apex necrosis virus) is a positive-strand rna virus in the genus torradovirus within the family secoviridae. tomarv is an emergent whitefly-transmitted virus that causes important diseases in tomato (solanum lycopersicum) in mexico. here, the genome sequence of the tomarv isolate m (tomarv-m) was determined. we engineered full-length cdna clones of the tomarv-m genomic rna (rna1 and rna2), separately, into a binary vector. coinfiltration of ... | 2016 | 26756828 |
| the nucleotide sequence of a polish isolate of tomato torrado virus. | a new virus was isolated from greenhouse tomato plants showing symptoms of leaf and apex necrosis in wielkopolska province in poland in 2003. the observed symptoms and the virus morphology resembled viruses previously reported in spain called tomato torrado virus (totv) and that in mexico called tomato marchitez virus (tomarv). the complete genome of a polish isolate wal'03 was determined using rt-pcr amplification using oligonucleotide primers developed against the totv sequences deposited in g ... | 2008 | 18781383 |
| tomato chocolate spot virus, a member of a new torradovirus species that causes a necrosis-associated disease of tomato in guatemala. | tomatoes in guatemala have been affected by a new disease, locally known as "mancha de chocolate" (chocolate spot). the disease is characterized by distinct necrotic spots on leaves, stems and petioles that eventually expand and cause a dieback of apical tissues. samples from symptomatic plants tested negative for infection by tomato spotted wilt virus, tobacco streak virus, tobacco etch virus and other known tomato-infecting viruses. a virus-like agent was sap-transmitted from diseased tissue t ... | 2010 | 20376682 |
| tomato marchitez virus, a new plant picorna-like virus from tomato related to tomato torrado virus. | a new virus was isolated from a tomato plant from the state of sinaloa in mexico. this plant showed symptoms locally known as 'marchitez disease': severe leaf necrosis, beginning at the base of the leaflets, and necrotic rings on the fruits. a virus was isolated from the infected plant consisting of isometric particles with a diameter of approximately 28 nm. the viral genome consists of two (+)ssrna molecules of 7221 (rna1) and 4898 nts (rna2). the viral capsid contains three coat proteins of 35 ... | 2008 | 17965923 |