| molecular cloning and characterization of an insecticidal toxin from pseudomonas taiwanensis. | an insecticidal toxin gene, tccc, was cloned from the recently discovered novel species pseudomonas taiwanensis using degenerate pcr and genomic walking. the dna sequence of the tccc gene (2,940 bp) has an open reading frame encoding 980 amino acids with a calculated molecular weight of 107.93 kda. the amino acid sequence alignment showed the highest sequence identity (41.2%) with the insecticidal toxin from pseudomonas entomophila. to examine the insecticidal functionality of the tccc gene prod ... | 2010 | 21062004 |
| engineering of pseudomonas taiwanensis vlb120 for constitutive solvent tolerance and increased specific styrene epoxidation activity. | the application of whole cells as biocatalysts is often limited by the toxicity of organic solvents, which constitute interesting substrates/products or can be used as a second phase for in situ product removal and as tools to control multistep biocatalysis. solvent-tolerant bacteria, especially pseudomonas strains, are proposed as promising hosts to overcome such limitations due to their inherent solvent tolerance mechanisms. however, potential industrial applications suffer from tedious, unpro ... | 2014 | 25128338 |
| characterization of an insecticidal toxin and pathogenicity of pseudomonas taiwanensis against insects. | pseudomonas taiwanensis is a broad-host-range entomopathogenic bacterium that exhibits insecticidal activity toward agricultural pests plutella xylostella, spodoptera exigua, spodoptera litura, trichoplusia ni and drosophila melanogaster. oral infection with different concentrations (od = 0.5 to 2) of wild-type p. taiwanensis resulted in insect mortality rates that were not significantly different (92.7%, 96.4% and 94.5%). the tccc protein, a component of the toxin complex (tc), plays an essenti ... | 2014 | 25144637 |
| enhanced biodegradation of pyrene and indeno(1,2,3-cd)pyrene using bacteria immobilized in cinder beads in estuarine wetlands. | two strains (pseudomonas taiwanensis pyr1 and acinetobacter baumannii inp1) were isolated from pah-contaminated liaohe estuarine wetland using enrichment. the cells of pyr1 and inp1 were immobilized in cinder beads for pyrene and indeno(1,2,3-cd)pyrene biodegradation in wetland. biodegradation of pyrene and indeno(1,2,3-cd)pyrene in soils from wetland was carried out in pots using free cells as well as those immobilized in cinder beads to ascertain the role of bioaugmentation. supported by the c ... | 2016 | 26632525 |
| the application of constitutively solvent-tolerant p. taiwanensis vlb120δcδttgv for stereospecific epoxidation of toxic styrene alleviates carrier solvent use. | for whole-cell biotransformations involving toxic organic compounds, two-liquid phase setups are typically applied employing an apolar extractive phase. bis(2-ethylhexyl)phthalate (behp) has proven to be an ideal solvent for stereospecific styrene epoxidation with recombinant e. coli, providing excellent extractive properties and a high biocompatibility. in eco-efficiency evaluations, behp, however, has been identified as a critical factor regarding costs and environmental impact. in this study, ... | 2017 | 28345250 |
| anti-influenza virus activity of high-mannose binding lectins derived from genus pseudomonas. | lectin pfl binding high-mannose glycan derived from pseudomonas fluorescens and other homologous lectins: pml derived from pseudomonas mandelii and ptl derived from pseudomonas taiwanensis were examined for antiviral activity. the cdna of these lectin genes were synthesized, cloned, expressed in escherichia coli. the expressed lectins were purified by gel filtrations, and supplied to cultures infected with several strains of influenza virus. these three lectins have inhibited propagation of infl ... | 2016 | 27374061 |
| making variability less variable: matching expression system and host for oxygenase-based biotransformations. | variability in whole-cell biocatalyst performance represents a critical aspect for stable and productive bioprocessing. in order to investigate whether and how oxygenase-catalyzed reactions are affected by such variability issues in solvent-tolerant pseudomonas, different inducers, expression systems, and host strains were tested for the reproducibility of xylene and styrene monooxygenase catalyzed hydroxylation and epoxidation reactions, respectively. significantly higher activity variations we ... | 2015 | 25877162 |
| biosurfactant production from pseudomonas taiwanensis l1011 and its application in accelerating the chemical and biological decolorization of azo dyes. | dye dispersion and the interaction efficiency between azoreductases and dye molecules are rate-limiting steps for the decolorization of azo dyes. in this study, a biosurfactant-producing strain, pseudomonas taiwanensis l1011, was isolated from crude oil. to increase the yield of the biosurfactant bs-l1011 from p. taiwanensis l1011, culture conditions were optimized including temperature, initial ph, carbon source, nitrogen source and c/n ratio. a maximum yield of 1.12g/l of bs-l1011 was obtained ... | 2017 | 28689070 |
| variability in subpopulation formation propagates into biocatalytic variability of engineered pseudomonas putida strains. | pivotal challenges in industrial biotechnology are the identification and overcoming of cell-to-cell heterogeneity in microbial processes. while the development of subpopulations of isogenic cells in bioprocesses is well described (intra-population variability), a possible variability between genetically identical cultures growing under macroscopically identical conditions (clonal variability) is not. a high such clonal variability has been found for the recombinant expression of the styrene mon ... | 2015 | 26483771 |
| involvement of type vi secretion system in secretion of iron chelator pyoverdine in pseudomonas taiwanensis. | rice bacterial blight caused by xanthomonas oryzae pv. oryzae (xoo) is one of the most destructive rice diseases worldwide. therefore, in addition to breeding disease-resistant rice cultivars, it is desirable to develop effective biocontrol agents against xoo. here, we report that a soil bacterium pseudomonas taiwanensis displayed strong antagonistic activity against xoo. using matrix-assisted laser desorption/ionization imaging mass spectrometry, we identified an iron chelator, pyoverdine, secr ... | 2016 | 27605490 |
| pseudomonas taiwanensis sp. nov., isolated from soil. | a novel gram-negative, rod-shaped, motile, non-spore-forming bacterial strain, cms(t), isolated from soil was characterized using phenotypic and molecular taxonomic methods. 16s rrna gene sequence analysis revealed that the organism belongs phylogenetically to the genus pseudomonas. pseudomonas monteilii, p. plecoglossicida and p. mosselii were the most closely related species, with 16s rrna gene sequence similarities to the respective type strains of 99.79, 99.73 and 99.59 %. relatively low gyr ... | 2010 | 19854877 |
| d-xylose assimilation via the weimberg pathway by solvent-tolerant pseudomonas taiwanensis vlb120. | the natural ability of pseudomonas taiwanensis vlb120 to use xylose as sole carbon and energy source offers a high potential for sustainable industrial biotechnology. in general, three xylose assimilation routes are reported for bacteria. to elaborate the metabolic capacity of p. taiwanensis vlb120 and to identify potential targets for metabolic engineering, an in silico/in vivo experiment was designed, allowing for discrimination between these pathways. kinetics of glucose and xylose degradatio ... | 2015 | 24934825 |
| a three-step method for analysing bacterial biofilm formation under continuous medium flow. | for the investigation and comparison of microbial biofilms, a variety of analytical methods have been established, all focusing on different growth stages and application areas of biofilms. in this study, a novel quantitative assay for analysing biofilm maturation under the influence of continuous flow conditions was developed using the interesting biocatalyst pseudomonas taiwanensis vlb120. in contrast to other tubular-based assay systems, this novel assay format delivers three readouts using a ... | 2015 | 25936379 |
| trophic regulation of autoaggregation in pseudomonas taiwanensis vlb120. | five mutants of pseudomonas taiwanensis vlb120δcegfp showed significant autoaggregation when growing on defined carbohydrates or gluconate, while they grew as suspended cells on complex medium and on organic acids like citrate and succinate. surprisingly, the respective mutations affected very different genes, although all five strains exhibited the same behaviour of aggregate formation. to elucidate the mechanism of the aggregative behaviour, the microbial adhesion to hydrocarbons (math) assay ... | 2016 | 26428239 |
| draft genome sequence of a caprolactam degrader bacterium: pseudomonas taiwanensis strain sj9. | pseudomonas taiwanensis strain sj9 is a caprolactam degrader, isolated from industrial wastewater in south korea and considered to have the potential for caprolactam bioremediation. the genome of this strain is approximately 6.2 mb (g+c content, 61.75%) with 6,010 protein-coding sequences (cds), of which 46% are assigned to recognized functional genes. this draft genome of strain sj9 will provide insights into the genetic basis of its caprolactam-degradation ability. | 2017 | 26991304 |
| hyperadherence of pseudomonas taiwanensis vlb120δc increases productivity of (s)-styrene oxide formation. | the attachment strength of biofilm microbes is responsible for the adherence of the cells to surfaces and thus is a critical parameter in biofilm processes. in tubular microreactors, aqueous-air segmented flow ensures an optimal oxygen supply and prevents excessive biofilm growth. however, organisms growing in these systems depend on an adaptation phase of several days, before mature and strong biofilms can develop. this is due to strong interfacial forces. in this study, a hyperadherent mutant ... | 2017 | 27411543 |
| quantifying the metabolome of pseudomonas taiwanensis vlb120: evaluation of hot and cold combined quenching/extraction approaches. | absolute quantification of free intracellular metabolites is a valuable tool in both pathway discovery and metabolic engineering. in this study, we conducted a comprehensive examination of different hot and cold combined quenching/extraction approaches to extract and quantify intracellular metabolites of pseudomonas taiwanensis (p. taiwanensis) vlb120 to provide a useful reference data set of absolute intracellular metabolite concentrations. the suitability of commonly used metabolomics tools in ... | 2017 | 28727413 |
| biodegradation of 2-hydroxyl-1,4 naphthoquinone (lawsone) by pseudomonas taiwanensis lh-3 isolated from activated sludge. | 2-hydroxy-1,4 naphthoquinone (lawsone) is widely used and induces environmental pollutions during its production and application. in the present study, a lawsone-degrading bacterium strain, lh-3 was successfully isolated from the activated sludge. based on the 16s rrna gene analysis, the strain lh-3 phylogenetically belonged to the pseudomonas taiwanensis. it could degrade 200 mg l-1 lawsone completely in 9 h with an inoculum quantity of 1% (v/v). the effects of environmental conditions on the d ... | 2017 | 28754900 |
| n-terminus determines activity and specificity of styrene monooxygenase reductases. | styrene monooxygenases (smos) are two-enzyme systems that catalyze the enantioselective epoxidation of styrene to (s)-styrene oxide. the fadh2 co-substrate of the epoxidase component (stya) is supplied by an nadh-dependent flavin reductase (styb). the genome of rhodococcus opacus 1cp encodes two smo systems. one system, which we define as e1-type, displays homology to the smo from pseudomonas taiwanensis vlb120. the other system, originally reported as a fused system (rostya2b), is defined as e2 ... | 2017 | 28888693 |
| biocatalytic conversion of cycloalkanes to lactones using an in-vivo cascade in pseudomonas taiwanensis vlb120. | chemical synthesis of lactones from cycloalkanes is a multi-step process challenged by limitations in reaction efficiency (conversion and yield), atom economy (by-products) and environmental performance. a heterologous pathway comprising novel enzymes with compatible kinetics was designed in pseudomonas taiwanensis vlb120 enabling in-vivo cascade for synthesizing lactones from cycloalkanes. the respective pathway included cytochrome p450 monooxygenase (chx), cyclohexanol dehydrogenase (cdh), and ... | 2018 | 28986995 |
| structural characterization of pyoverdines produced by pseudomonas putida kt2440 and pseudomonas taiwanensis vlb120. | the previously unknown sequences of several pyoverdines (pvd) produced by a biotechnologically-relevant bacterium, namely, pseudomonas taiwanensis vlb120, were characterized by high performance liquid chromatography (hplc)-high resolution mass spectrometry (hrms). the same structural characterization scheme was checked before by analysis of pseudomonas sp. putida kt2440 samples with known pvds. a new sample preparation strategy based on solid-phase extraction was developed, requiring significant ... | 2017 | 28631237 |
| as(v) resistance and reduction by bacteria and their performances in as removal from as-contaminated soils. | bacteria capable of arsenate [as(v)] reduction can be used for remediation of as-contaminated soils via bio-extraction. in this study, as-resistant bacteria were isolated and their abilities to resist and reduce as(v) as well as as bio-extracted from soils naturally contaminated with as were studied. the results indicated that three isolates (2-2, 4-3, and 8-5) showed greater abilities to resist as(v) than other isolates. when the isolates were exposed to 10 mg l(-1) as(v), as(v) contents decrea ... | 2017 | 28676887 |
| continuous cyclohexane oxidation to cyclohexanol using a novel cytochrome p450 monooxygenase from acidovorax sp. chx100 in recombinant p. taiwanensis vlb120 biofilms. | the applications of biocatalysts in chemical industries are characterized by activity, selectivity, and stability. one key strategy to achieve high biocatalytic activity is the identification of novel enzymes with kinetics optimized for organic synthesis by nature. the isolation of novel cytochrome p450 monooxygenase genes from acidovorax sp. chx100 and their functional expression in recombinant pseudomonas taiwanensis vlb120 enabled efficient oxidation of cyclohexane to cyclohexanol. although i ... | 2016 | 26153144 |
| growth of pseudomonas taiwanensis vlb120∆c biofilms in the presence of n-butanol. | biocatalytic processes often encounter problems due to toxic reactants and products, which reduce biocatalyst viability. thus, robust organisms capable of tolerating or adapting towards such compounds are of high importance. this study systematically investigated the physiological response of pseudomonas taiwanensis vlb120∆c biofilms when exposed to n-butanol, one of the potential next generation biofuels as well as a toxic substance using microscopic and biochemical methods. initially p. taiwan ... | 2016 | 27696696 |
| continuous multistep synthesis of perillic acid from limonene by catalytic biofilms under segmented flow. | the efficiency of biocatalytic reactions involving industrially interesting reactants is often constrained by toxification of the applied biocatalyst. here, we evaluated the combination of biologically and technologically inspired strategies to overcome toxicity-related issues during the multistep oxyfunctionalization of (r)-(+)-limonene to (r)-(+)-perillic acid. pseudomonas putida gs1 catalyzing selective limonene oxidation via the p-cymene degradation pathway and recombinant pseudomonas taiwan ... | 2017 | 27530691 |
| pseudomonas guariconensis sp. nov., isolated from rhizospheric soil. | we isolated a bacterial strain designated pcavu11(t) in the course of a study of phosphate-solubilizing bacteria occurring in rhizospheric soil of vigna unguiculata (l.) walp. in guárico state, venezuela. the 16s rrna gene sequence had 99.2 % sequence similarity with respect to the most closely related species, pseudomonas taiwanensis, and 99.1 % with respect to pseudomonas entomophila, pseudomonas plecoglossicida and pseudomonas monteilii, on the basis of which pcavu11(t) was classified as repr ... | 2013 | 23847284 |