| rapid detection of antigenic diversity of akabane virus isolates by dot immunobinding assay using neutralizing monoclonal antibodies. | akabane (aka) virus is an arthropod-borne virus belonging to the simbu group of the genus bunyavirus. neutralizing monoclonal antibodies (mabs) against aka virus were prepared, and the neutralizing epitopes of the virus were defined by competitive binding assay. five distinct antigenic domains were identified and were designated a, b, c, d, and e. domains a and c consisted of two epitopes each. it was demonstrated that seven neutralizing epitopes exist on the g1 glycoprotein of aka virus. dot im ... | 1998 | 9521142 |