| first molecular detection of piroplasm infection in pet dogs from gansu, china. | babesiosis, the hemolytic disease caused by babesia, which is a tick-transmitted obligate intraerythrocytic protozoan parasite. this disease is responsible for significant mortality and morbidity rates and enormous economic losses to the livestock industry in tropical and subtropical regions worldwide. in this study, blood samples were collected from 141 pet dogs from gansu, china, and analyzed for babesia or theileria spp. infection using specific pcr and sequencing based on 18s rrna gene fragm ... | 2017 | 28638376 |
| identification of 12 piroplasms infecting ten tick species in china using reverse line blot hybridization. | piroplasmosis, a disease of domestic and wild animals is caused by tick-borne protozoa in the genera of theileria and babesia. there was limited information available about the prevalence of piroplasmosis in ticks in china. to assess potential threat of piroplasmosis in china, we investigated the infections of ovine and bovine babesia and theileria species in ticks collected from cattle, yaks, sheep, horses and camels in several regions of china where tick-borne diseases have been reported. a to ... | 2017 | 28355109 |
| rapid identification and differentiation of theileria sergenti and theileria sinensis using a loop-mediated isothermal amplification (lamp) assay. | the present study developed and validated a species-specific loop-mediated isothermal amplification (lamp) assay for the rapid detection and discrimination of two benign bovine theileria species -t. sergenti and t. sinensis. the lamp assay is inexpensive and easy to perform and involves a rapid reaction-the amplification can be performed in 55 min or 50 min under isothermal conditions of 61°c or 63°c, respectively, by employing a set of four species-specific primer mixtures. the results can be c ... | 2013 | 22947247 |
| seroprevalence of bovine theileriosis in northern china. | bovine theileriosis is a common disease transmitted by ticks, and can cause loss of beef and dairy cattle worldwide. here, an indirect enzyme-linked immunosorbent assay (ielisa) based on theileria luwenshuni surface protein (tlsp) was developed and used to carry out a seroepidemiological survey of bovine theileriosis in northern china. | 2016 | 27863521 |
| detecting and differentiating theileria sergenti and theileria sinensis in cattle and yaks by pcr based on major piroplasm surface protein (mpsp). | theileria sergenti and theileria sinensis are closely related members of benign theileria species found in cattle and yaks in china. they are morphologically indistinguishable. a polymerase chain reaction (pcr) targeting major piroplasm surface protein of t. sergenti and t. sinensis was developed in this study. the newly developed oligonucleotide primer set was able to specifically amplify the dna of t. sinensis and in conjunction with primers for t. sergenti and these two species could be detec ... | 2010 | 20685208 |
| development and evaluation of a loop-mediated isothermal amplification method for diagnosis of tropical theileriosis. | a loop-mediated isothermal amplification (lamp) assay was developed and evaluated for diagnosis of tropical theileriosis. a set of six primers was designed based on the unique gene of theileria annulata (theileria annulata strain ankara hypothetical protein (genedb ta04795). the protocol for the reaction was setup and the specificity and sensitivity of the assay were established. the specificity experiment showed that lamp primers amplified t. annulata dna successfully, while no amplification wa ... | 2008 | 18666968 |
| phylogeny of theileria buffeli genotypes identified in the south african buffalo (syncerus caffer) population. | theileria buffeli/orientalis is a group of benign and mildly pathogenic species of cattle and buffalo in various parts of the world. in a previous study, we identified t. buffeli in blood samples originating from the african buffalo (syncerus caffer) in the hluhluwe-imfolozi game park (hip) and the addo elephant game park (aegp) in south africa. the aim of this study was to characterise the 18s rrna gene and complete internal transcribed spacer (its1-5.8s-its2) region of t. buffeli samples, and ... | 2014 | 25002308 |
| molecular detection and characterization of theileria infection in cattle and yaks from tibet plateau region, china. | theileriosis continues to threaten the livestock industry worldwide, but comprehensive epidemiological surveys for this disease have not been conducted in the tibet plateau region, china. in this study, we screened 154 cattle blood samples from the tibet plateau region (lhasa, lhoka, and tianzhu), china, for detection of theileria pathogens by polymerase chain reaction (pcr) with species-specific primers. the results revealed that the prevalence was 6.9 % (2/29) for theileria orientalis and 27.6 ... | 2016 | 27000088 |
| additional data for a new theileria sp. from china based on the sequences of ribosomal rna internal transcribed spacers. | theileria sinensis was recently isolated and named as an independent theileria species that infects cattle in china. to date, this parasite has been described based on its morphology, transmission and molecular studies, indicating that it should be classified as a distinct species. to test the validity of this taxon, the two internal transcribed spacers (its1 and its2) and the 5.8s rrna gene were cloned and sequenced from three t. sinensis isolates. the complete its sequences were compared with ... | 2013 | 23220031 |
| loop-mediated isothermal amplification (lamp) method based on two species-specific primer sets for the rapid identification of chinese babesia bovis and b. bigemina. | bovine babesiosis is a tick-transmitted hemoprotozoan disease that is mainly caused by babesia bovis and/or babesia bigemina and is characterized by significant morbidity and mortality worldwide. this disease is widespread in most parts of china. however, it is difficult to rapidly discriminate between the b. bovis and b. bigemina species. to detect and distinguish these species, a loop-mediated isothermal amplification (lamp) platform that targets specific sequences of the internal transcribed ... | 2012 | 22849831 |
| evaluating an indirect rmpsp enzyme-linked immunosorbent assay for the detection of bovine theileria infection in china. | bovine theileriosis, a tick-borne protozoan disease caused by theileria annulata, theileria orientalis and theileria sinensis, is widespread in china and is a serious economic problem for the chinese livestock industry. in this study, recombinant major piroplasma surface proteins (mpsp) of t. annulata, t. orientalis and t. sinensis based on mpsp genes were expressed in escherichia coli bl21(de3). the immunogenicity and specificity of the three purified recombinant mpsp proteins were evaluated wi ... | 2017 | 27942962 |
| molecular assays reveal the presence of theileria spp. and babesia spp. in asian water buffaloes (bubalus bubalis, linnaeus, 1758) in the amazon region of brazil. | approximately 50% of buffalo herds in brazil are located in pará state in northern brazil. there are several properties where cattle and buffalo live and graze together, and thus, buffalo pathogens may threaten the health of cattle and vice versa. therefore, knowledge of infectious agents of buffalo is essential for maintaining healthy livestock. clinical disease caused by theileria and babesia parasites in the asian water buffalo is not common, although these animals may act as reservoir hosts, ... | 2016 | 27344507 |