| molecular variability of sweet potato feathery mottle virus and other potyviruses infecting sweet potato in peru. | several potyviruses are found infecting sweet potato (ipomoea batatas) in peru, of which sweet potato feathery mottle virus (spfmv, genus potyvirus) is the most common. however, sequence data for these viruses are not available from peru. in this study, the 3'-terminal approximately 1,800 nucleotide sequences of 17 potyvirus samples collected from the six main sweet potato-producing areas of peru over the past 20 years were determined and analyzed. results of sequence comparisons and phylogeneti ... | 2008 | 18172571 |
| phylogenetic relationships of closely related potyviruses infecting sweet potato determined by genomic characterization of sweet potato virus g and sweet potato virus 2. | complete nucleotide sequences of sweet potato virus g (spvg) and sweet potato virus 2 (spv2) were determined to be 10,800 and 10,731 nucleotides, respectively, excluding the 3'-poly(a) tail. their genomic organizations are typical of potyviruses, encoding a polyprotein which is likely cleaved into 10 mature proteins by three viral proteinases. conserved motifs of orthologous proteins of viruses in the genus potyvirus are found in corresponding positions of both viruses. pairwise comparisons of i ... | 2012 | 22562225 |
| the current incidence of viral disease in korean sweet potatoes and development of multiplex rt-pcr assays for simultaneous detection of eight sweet potato viruses. | sweet potato is grown extensively from tropical to temperate regions and is an important food crop worldwide. in this study, we established detection methods for 17 major sweet potato viruses using single and multiplex rt-pcr assays. to investigate the current incidence of viral diseases, we collected 154 samples of various sweet potato cultivars showing virus-like symptoms from 40 fields in 10 korean regions, and analyzed them by rt-pcr using specific primers for each of the 17 viruses. of the ... | 2014 | 25506306 |
| detection of sweet potato virus c, sweet potato virus 2 and sweet potato feathery mottle virus in portugal. | field sweet potato plants showing virus-like symptoms, as stunting, leaf distortion, mosaic and chlorosis, were collected in southwest portugal and tested for the presence of four potyviruses, sweet potato virus c (spvc), sweet potato virus 2 (spv2), sweet potato feathery mottle virus (spfmv), sweet potato virus g (spvg), and the crinivirus sweet potato chlorotic stunt virus (spcsv). dsrna fractions were extracted from symptomatic leaves and used as templates in single and multiplex rt-pcr assay ... | 2015 | 26104336 |
| variation in virus symptom development and root architecture attributes at the onset of storage root initiation in 'beauregard' sweetpotato plants grown with or without nitrogen. | it has been shown that virus infections, often symptomless, significantly limit sweetpotato productivity, especially in regions characterized by low input agricultural systems. in sweetpotatoes, the successful emergence and development of lateral roots (lrs), the main determinant of root architecture, determines the competency of adventitious roots to undergo storage root initiation. this study aimed to investigate the effect of some plant viruses on root architecture attributes during the onset ... | 2014 | 25243579 |
| molecular characterization of five potyviruses infecting korean sweet potatoes based on analyses of complete genome sequences. | sweet potatoes (ipomea batatas l.) are grown extensively, in tropical and temperate regions, and are important food crops worldwide. in korea, potyviruses, including sweet potato feathery mottle virus (spfmv), sweet potato virus c (spvc), sweet potato virus g (spvg), sweet potato virus 2 (spv2), and sweet potato latent virus (splv), have been detected in sweet potato fields at a high (~95%) incidence. in the present work, complete genome sequences of 18 isolates, representing the five potyviruse ... | 2015 | 26673876 |
| population dynamics of three aphid species (hemiptera: aphididae) on four ipomoea spp. infected or noninfected with sweetpotato potyviruses. | three aphid species, aphis gossypii glover and myzus persicae (sulzer) (efficient sweetpotato potyvirus vectors) and rhopalosiphum padi (l.) (an inefficient vector), are commonly found in sweet potato, ipomoea batatas (l.), in louisiana. field-grown sweet potatoes are naturally infected with several potyviruses: sweet potato feathery mottle virus, sweet potato virus g, and sweet potato virus 2. thus, these aphids commonly encounter virus-infected hosts. what is not known is how each of these aph ... | 2013 | 24020267 |
| simultaneous detection and differentiation of four closely related sweet potato potyviruses by a multiplex one-step rt-pcr. | four closely related potyviruses, sweet potato feathery mottle virus (spfmv), sweet potato virus c (spvc), sweet potato virus g (spvg) and/or sweet potato virus 2 (spv2), are involved in sweet potato virus disease complexes worldwide. identification and detection of these viruses are complicated by high similarity among their genomic sequences, frequent occurrence as mixed infections and low titer in many sweet potato cultivars. a one-tube multiplex reverse transcription-pcr (mrt-pcr) assay was ... | 2012 | 22827958 |
| biological and molecular variability among geographically diverse isolates of sweet potato virus 2. | sweet potato virus 2 (spv2) is a tentative member of the genus potyvirus, family potyviridae. in addition to the type isolate of spv2 recently characterised in greater detail, twelve additional isolates of this virus were obtained from sweet potato clones originating from china, portugal, south africa and zambia. sequences of the coat protein (cp) gene and 3' non-translated region (ntr) were determined. comparisons of the cp gene sequences of these isolates revealed nucleotide and amino acid seq ... | 2007 | 17136503 |
| complete genome sequences of the potyvirus sweet potato virus 2 from east timor and australia. | we present here the first complete genome sequences of sweet potato virus 2 (spv2) from sweet potato in australia and east timor, and compare these with five complete spv2 genome sequences from south korea and one each from spain and the united states. both were closely related to spv2 genomes from south korea, spain, and the united states. | 2016 | 27257208 |