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quantification of bovine igg, igm and iga antibodies to clostridium perfringens b-toxin by enzyme immunoassay i. preparturient immunization for enhancement of passive transfer of immunity.a quantitative competitive binding "triple-sandwich" enzyme immunoassay was developed and used to evaluated pathogen/class-specific antibody responses in holstein-friesian cows vaccinated against clostridium perfringens b-toxin. vaccination of cows at six weeks and again at two weeks prepartum increased pathogen-specific igg levels in each dam's colostrum and respective calf's serum. pathogen-specific igg and igm concentrations in dams' sera and colostra were related to subsequent pathogen-speci ...19836310852
quantification of bovine igg, igm and iga antibodies to clostridium perfringens b-toxin by enzyme immunoassay ii systemic effects of maternally derived antibodies on immunization of newborn calves.a quantitative competitive binding "triple sandwich" enzyme immunoassay was used to evaluate pathogen/class-specific antibody responses in forty-eight holstein-friesian calves vaccinated against clostridium perfringens b-toxin at various ages postpartum. to assure a wide serum pathogen/class-specific immunoglobulin concentration range, one-half of all calves were from previously vaccinated dams. calves initially vaccinated at three days produced both a primary and secondary pathogen-specific ant ...19836310853
[action of penicillin and streptomycin on clostridium perfringens b, c and d and their toxin]. 195313089272
demonstration of passive protection in neonatal calves against colibacillosis following immunization of pregnant heifers at 3 months of gestation.enterotoxigenic escherichia coli is one of the primary etiologic agents for diarrhea in neonatal calves. immunization of dams can provide passive protection in neonatal calves; antibodies transferred through colostrum block colonization of bacteria, thereby preventing disease. in this study, healthy pregnant heifers were vaccinated at approximately 3 months of gestation with either a polyvalent oil-adjuvanted vaccine containing inactivated coronavirus, rotavirus, e. coli k99 subunit antigen, and ...200819177333
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