| [oligonucleotide primers for detection and amplification of the emoa gene encoding bacterial ethylenediaminetetraacetate monooxygenase]. | a system of primers was designed on the basis of analysis of nucleotide sequences of the emoa gene encoding ethylenediaminetetraacetate (edta) monooxygenase, which are deposited in genbank. this system of primers makes it possible to obtain emoa gene fragments approximately 750 bp long for bacterial destructors of edta. polymerase chain reaction (pcr) of total dna isolated from enrichment and pure cultures showed that this system can be effectively used for detecting the emoa gene in representat ... | 2008 | 18924405 |
| chelativorans multitrophicus gen. nov., sp. nov. and chelativorans oligotrophicus sp. nov., aerobic edta-degrading bacteria. | two previously isolated strains (dsm 9103(t) and lpm-4(t)) able to grow with edta (facultatively and obligately, respectively) as the source of carbon, nitrogen and energy were investigated in order to clarify their taxonomic positions. the strains were strictly aerobic, gram-negative, asporogenous and non-motile rods that required biotin for growth. reproduction occurred by binary fission. the strains were mesophilic and neutrophilic. their major fatty acids were summed feature 7 (consisting of ... | 2010 | 19666787 |
| [metabolism characteristics of chelativorans oligotrophicus by two-phase growth on the mixture of edta and glucose]. | the obligate destructor of ethylene diamine tetraacetate--a culture of chelativorans oligotrophicus lpm-4--did not grow on a medium with glucose, but it was good to use it under cultivation on a mixture with edta after considerable decrease of the edta concentration in the medium (two-phase growth). strong inhibition of hexokinase and glucose 6-phosphate dehydrogenase in cell exracts 4 mm edta was revealed. using edta, cells accumulated polyphosphates whose rate decreased during glucose utilizat ... | 2009 | 19845287 |
| genetic and functional characterization of a yet-unclassified rhizobial dtr (dna-transfer-and-replication) region from a ubiquitous plasmid conjugal system present in sinorhizobium meliloti, in sinorhizobium medicae, and in other nonrhizobial gram-negative bacteria. | rhizobia are gram-negative bacteria that live in soils and associate with leguminous plants to establish nitrogen-fixing symbioses. the ability of these bacteria to undergo horizontal gene transfer (hgt) is thought to be one of the main features to explain both the origin of their symbiotic life-style and the plasticity and dynamics of their genomes. in our laboratory we have previously characterized at the species level the non-psym plasmid mobilome in sinorhizobium meliloti, the symbiont of me ... | 2012 | 22233546 |