| genetic divergence and classification of nonstructural protein 1 among porcine rotaviruses of species b. | porcine rotavirus b (rvb) has been often detected in diarrhea of suckling and weaned pigs. moreover, epidemiological studies using elisa demonstrated high antibody prevalence in sera from sows, indicating that rvb infections are widespread. because it is difficult to serially propagate rvbs in cell culture, genetic analysis of rna segments of porcine rvbs other than those encoding vp7 and nsp2 has been scarcely performed. we conducted sequence and phylogenetic analyses focusing on nonstructural ... | 2011 | 21832005 |
| sequence and phylogenetic analyses of nonstructural protein 2 genes of species b porcine rotaviruses detected in japan during 2001-2009. | porcine rotavirus b (rvb) has been often detected in diarrhea of suckling and weaned pigs. because it is difficult to serially cultivate rvbs in cell culture, the number of available sequence data for rna segments other than vp7 and nsp1 in especially porcine rvbs is still limited. we performed genetic analysis focusing on nonstructural protein 2 (nsp2) using several porcine rvb strains, which were detected in diarrheic feces collected around japan during 2001-2009. comparison of nsp2 nucleotide ... | 2012 | 22248642 |
| longitudinal surveillance of porcine rotavirus b strains from the united states and canada andin silicoidentification of antigenically important sites. | rotavirus b (rvb) is an important swine pathogen, but control and prevention strategies are limited without an available vaccine. to develop a subunit rvb vaccine with maximal effect, we characterized the amino acid sequence variability and predicted antigenicity of rvb viral protein 7 (vp7), a major neutralizing antibody target, from clinically infected pigs in the united states and canada. we identified genotype-specific antigenic sites that may be antibody neutralization targets. while some a ... | 2017 | 29207506 |
| phylogenetic analysis of nonstructural protein 5 (nsp5) gene sequences in porcine rotavirus b strains. | porcine rotavirus b (rvb) has frequently been detected in the diarrhea of suckling and weaned pigs. because it is difficult to propagate rvbs serially in cell culture, little genetic information about rna segments other than vp7, nsp1 and nsp2 is available for porcine rvbs. we conducted a phylogenetic analysis focusing on nonstructural protein 5 (nsp5) using 22 porcine rvb strains, which were identified in fecal samples collected around japan. sequence analysis showed that nsp5 genes of rvbs con ... | 2012 | 22871651 |