| a practical guide to build de-novo assemblies for single tissues of non-model organisms: the example of a neotropical frog. | whole genome sequencing (wgs) is a very valuable resource to understand the evolutionary history of poorly known species. however, in organisms with large genomes, as most amphibians, wgs is still excessively challenging and transcriptome sequencing (rna-seq) represents a cost-effective tool to explore genome-wide variability. non-model organisms do not usually have a reference genome and the transcriptome must be assembled de-novo. we used rna-seq to obtain the transcriptomic profile for oreoba ... | 2017 | 28879061 |